IT1167077B - Sistema di assorbimento di colorane metacromatico ed emettitore di luce fluorescente per la determinazione differenziale degli stadi di sviluppo di cellule granulocitiche neutrofile ed altri leucociti - Google Patents

Sistema di assorbimento di colorane metacromatico ed emettitore di luce fluorescente per la determinazione differenziale degli stadi di sviluppo di cellule granulocitiche neutrofile ed altri leucociti

Info

Publication number
IT1167077B
IT1167077B IT47700/83A IT4770083A IT1167077B IT 1167077 B IT1167077 B IT 1167077B IT 47700/83 A IT47700/83 A IT 47700/83A IT 4770083 A IT4770083 A IT 4770083A IT 1167077 B IT1167077 B IT 1167077B
Authority
IT
Italy
Prior art keywords
neutrrophile
metacromatic
colorane
leukocytes
stages
Prior art date
Application number
IT47700/83A
Other languages
English (en)
Other versions
IT8347700A0 (it
Inventor
Lawrence Kass
Original Assignee
Lawrence Kass
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Lawrence Kass filed Critical Lawrence Kass
Publication of IT8347700A0 publication Critical patent/IT8347700A0/it
Application granted granted Critical
Publication of IT1167077B publication Critical patent/IT1167077B/it

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G01N21/6458—Fluorescence microscopy
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5094—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for blood cell populations
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • G01N2001/305—Fixative compositions

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Hematology (AREA)
  • Cell Biology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Ecology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Optics & Photonics (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
IT47700/83A 1982-03-09 1983-02-11 Sistema di assorbimento di colorane metacromatico ed emettitore di luce fluorescente per la determinazione differenziale degli stadi di sviluppo di cellule granulocitiche neutrofile ed altri leucociti IT1167077B (it)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US06/356,578 US4500509A (en) 1981-03-11 1982-03-09 Metachromatic dye sorption and fluorescent light emmisive means for differential determination of developmental stages of neutrophilic granulocytic cells and other leukocytes

Publications (2)

Publication Number Publication Date
IT8347700A0 IT8347700A0 (it) 1983-02-11
IT1167077B true IT1167077B (it) 1987-05-06

Family

ID=23402036

Family Applications (1)

Application Number Title Priority Date Filing Date
IT47700/83A IT1167077B (it) 1982-03-09 1983-02-11 Sistema di assorbimento di colorane metacromatico ed emettitore di luce fluorescente per la determinazione differenziale degli stadi di sviluppo di cellule granulocitiche neutrofile ed altri leucociti

Country Status (7)

Country Link
US (1) US4500509A (it)
JP (1) JPS58166261A (it)
CA (1) CA1205365A (it)
DE (1) DE3304795A1 (it)
FR (1) FR2523310B2 (it)
GB (1) GB2116712B (it)
IT (1) IT1167077B (it)

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4615878A (en) * 1980-03-12 1986-10-07 Lawrence Kass Metachromatic dye sorption means for differential determination of sub-populations of lymphocytes
DE3567280D1 (en) * 1984-04-27 1989-02-09 Lawrence Kass Identification of myeloblasts and other immature granulocytic cells
US4714606A (en) * 1984-05-15 1987-12-22 Cytocolor Incorporated Method of staining and identifying cells and compositions thereof
EP0183717B1 (en) * 1984-05-15 1989-11-23 Cytocolor Inc. Single dye replacement for romanowsky plural dye variable compositions in human biopsy specimen constituent identifications
US4751179A (en) * 1984-05-31 1988-06-14 Coulter Electronics, Inc. Method and reagents for differential determination of four populations of leukocytes in blood
US4622291A (en) * 1984-06-11 1986-11-11 The United States Of America As Represented By The Department Of Health And Human Services Method and device for quantitative end point determination in immunofluorescence using microfluorophotometry
EP0191850A1 (en) * 1984-08-09 1986-08-27 Lawrence Kass Differentiation, identification and enumeration of neuthrophils, erythroblast and sub-populations of lymphopcytes with basic blue 141
US4707451A (en) * 1985-09-18 1987-11-17 Becton, Dickinson And Company Detection of reticulocytes
US4810487A (en) * 1986-01-02 1989-03-07 Cytocolor, Inc. Method of identifying polymethine stained lymphocyte subpopulations and compositions thereof
DE68927479T2 (de) * 1988-05-02 1997-04-03 Phanos Tech Inc Verbindungen, zusammensetzungen und verfahren zum binden von bio-affektions-substanzen an oberflächenmembranen von bioteilchen
JP2683848B2 (ja) * 1991-02-15 1997-12-03 日本光電工業株式会社 血液モデル
US5407794A (en) * 1992-09-08 1995-04-18 Cytocolor Inc. Oxazine stained lymphocytes and method
DE4309328C2 (de) * 1993-03-18 1998-03-12 Volker Ost Verfahren zur Differenzierung, Konzentrationsbestimmung und Sortierung von Erythrozyten, Thrombozyten und Leukozyten
US5843657A (en) * 1994-03-01 1998-12-01 The United States Of America As Represented By The Department Of Health And Human Services Isolation of cellular material under microscopic visualization
US6251467B1 (en) 1994-03-01 2001-06-26 The United States Of America As Represented By The Department Of Health And Human Services Isolation of cellular material under microscopic visualization
US5843644A (en) * 1994-03-01 1998-12-01 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Isolation of cellular material under microscopic visualization using an adhesive/extraction reagent tipped probe
DE19503434A1 (de) * 1995-02-03 1996-08-08 Beiersdorf Ag Verfahren zur Bestimmung der Adhäsion von Mikroorganismen und der antiadhäsiven Wirkung von Stoffen
EP0973873B1 (en) * 1997-04-08 2006-06-07 Pall Corporation Method of harvesting rare cells from blood products
US6567214B2 (en) * 1997-09-04 2003-05-20 Andrew E. Lorincz Microscope slide having culture media and method for use
DE10124340A1 (de) * 2001-05-18 2002-12-05 Fraunhofer Ges Forschung Verfahren zur Analyse einer biologischen Probe
ES2588223T3 (es) * 2008-03-21 2016-10-31 Abbott Point Of Care, Inc. Procedimiento para el análisis de células individuales o partículas en la sangre usando fluorescencia y absorción de un colorante
CN105051538B (zh) 2012-12-17 2018-06-15 伦珂德克斯有限公司 用于检测生物状况的系统和方法

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BE403179A (it) * 1933-05-16
US3327119A (en) * 1964-03-26 1967-06-20 Ibm Method and apparatus for detecting cancer cells
JPS5239055B1 (it) * 1966-05-24 1977-10-03
US3617185A (en) * 1968-08-19 1971-11-02 Gaf Corp Stable highly concentrated solutions of basic dyes
US3684377A (en) * 1970-07-13 1972-08-15 Bio Physics Systems Inc Method for analysis of blood by optical analysis of living cells
US4146604A (en) * 1973-05-31 1979-03-27 Block Engineering, Inc. Differential counting of leukocytes and other cells
US3916205A (en) * 1973-05-31 1975-10-28 Block Engineering Differential counting of leukocytes and other cells
CA1024048A (en) * 1973-10-29 1978-01-10 Richard Sternheimer Urinary sediment stain
US3883247A (en) * 1973-10-30 1975-05-13 Bio Physics Systems Inc Method for fluorescence analysis of white blood cells
US3916197A (en) * 1973-11-28 1975-10-28 Particle Technology Inc Method and apparatus for classifying biological cells
US3899297A (en) * 1973-12-19 1975-08-12 Block Engineering Biological staining technique and mixture thereof
US3985500A (en) * 1974-08-21 1976-10-12 Gaf Corporation Black dye formulation
DE2515966C3 (de) * 1975-04-11 1979-11-22 Boehringer Mannheim Gmbh, 6800 Mannheim Vorgefärbte Objektträger für die Blutunte rsuchung
DE2962866D1 (en) * 1978-03-09 1982-07-08 Merck Patent Gmbh Method for determining leukaemic cells
US4400370A (en) * 1980-03-12 1983-08-23 Lawrence Kass Metachromatic dye sorption means for differential determination of leukocytes
US4581223A (en) * 1980-03-12 1986-04-08 Lawrence Kass Individual leukocyte determination by means of differential metachromatic dye sorption
CA1155041A (en) * 1980-04-21 1983-10-11 Michael E. Jolley Fluorescent nucleic acid stains

Also Published As

Publication number Publication date
CA1205365A (en) 1986-06-03
DE3304795A1 (de) 1983-09-22
GB8303897D0 (en) 1983-03-16
GB2116712A (en) 1983-09-28
JPS58166261A (ja) 1983-10-01
DE3304795C2 (it) 1992-06-04
IT8347700A0 (it) 1983-02-11
GB2116712B (en) 1985-07-10
FR2523310A2 (fr) 1983-09-16
US4500509A (en) 1985-02-19
FR2523310B2 (fr) 1986-12-19
JPH0473104B2 (it) 1992-11-19

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