IT8247950A0 - Sistema e composizione di coloranti metacromatici per la determinazione differenziale degli stadi di sviluppo di cellule granulocitiche neutrofile ed altri leucociti - Google Patents
Sistema e composizione di coloranti metacromatici per la determinazione differenziale degli stadi di sviluppo di cellule granulocitiche neutrofile ed altri leucocitiInfo
- Publication number
- IT8247950A0 IT8247950A0 IT8247950A IT4795082A IT8247950A0 IT 8247950 A0 IT8247950 A0 IT 8247950A0 IT 8247950 A IT8247950 A IT 8247950A IT 4795082 A IT4795082 A IT 4795082A IT 8247950 A0 IT8247950 A0 IT 8247950A0
- Authority
- IT
- Italy
- Prior art keywords
- neutrophile
- leukocytes
- composition
- development stages
- differential determination
- Prior art date
Links
- 210000004027 cell Anatomy 0.000 title 1
- 238000011161 development Methods 0.000 title 1
- 210000000265 leukocyte Anatomy 0.000 title 1
- 230000003458 metachromatic effect Effects 0.000 title 1
- 239000000203 mixture Substances 0.000 title 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5094—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for blood cell populations
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N2001/305—Fixative compositions
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Urology & Nephrology (AREA)
- Chemical & Material Sciences (AREA)
- Cell Biology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Hematology (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Ecology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Biotechnology (AREA)
- Analytical Chemistry (AREA)
- General Physics & Mathematics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US06/242,662 US4400370A (en) | 1980-03-12 | 1981-03-11 | Metachromatic dye sorption means for differential determination of leukocytes |
Publications (2)
Publication Number | Publication Date |
---|---|
IT8247950A0 true IT8247950A0 (it) | 1982-03-09 |
IT1148123B IT1148123B (it) | 1986-11-26 |
Family
ID=22915693
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
IT47950/82A IT1148123B (it) | 1981-03-11 | 1982-03-09 | Sistema e composizione di coloranti metacromatici per la determinazione differenziale degli stadi di sviluppo di cellule granulocitiche neutro file ed altri leucociti |
Country Status (7)
Country | Link |
---|---|
US (1) | US4400370A (it) |
JP (1) | JPS57161550A (it) |
CA (1) | CA1191079A (it) |
DE (1) | DE3208629A1 (it) |
FR (1) | FR2501865B2 (it) |
GB (1) | GB2095402B (it) |
IT (1) | IT1148123B (it) |
Families Citing this family (32)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4581223A (en) * | 1980-03-12 | 1986-04-08 | Lawrence Kass | Individual leukocyte determination by means of differential metachromatic dye sorption |
US4615878A (en) * | 1980-03-12 | 1986-10-07 | Lawrence Kass | Metachromatic dye sorption means for differential determination of sub-populations of lymphocytes |
US4500509A (en) * | 1981-03-11 | 1985-02-19 | Lawrence Kass | Metachromatic dye sorption and fluorescent light emmisive means for differential determination of developmental stages of neutrophilic granulocytic cells and other leukocytes |
DE3238353A1 (de) * | 1982-10-15 | 1984-04-19 | Max Planck Gesellschaft zur Förderung der Wissenschaften e.V., 3400 Göttingen | Verfahren zur simultanen quantitativen bestimmung der blutzellen und reagenz hierfuer |
US4508821A (en) * | 1983-07-05 | 1985-04-02 | Becton Dickinson And Company | Detection of white cell associated bacteria with a fluorescent dye |
WO1985005180A1 (en) * | 1984-04-27 | 1985-11-21 | Merck Patent Gmbh | Identification of myeloblasts and other immature granulocytic cells |
WO1985005446A1 (en) * | 1984-05-15 | 1985-12-05 | Merck Patent Gmbh | Single dye replacement for romanowsky plural dye variable compositions in human biopsy specimen constituent identifications |
US4714606A (en) * | 1984-05-15 | 1987-12-22 | Cytocolor Incorporated | Method of staining and identifying cells and compositions thereof |
EP0191850A1 (en) * | 1984-08-09 | 1986-08-27 | Lawrence Kass | Differentiation, identification and enumeration of neuthrophils, erythroblast and sub-populations of lymphopcytes with basic blue 141 |
US4810487A (en) * | 1986-01-02 | 1989-03-07 | Cytocolor, Inc. | Method of identifying polymethine stained lymphocyte subpopulations and compositions thereof |
JPH076979B2 (ja) * | 1986-09-10 | 1995-01-30 | 東亜医用電子株式会社 | フロ−サイトメトリ−による白血球分類に使用される試薬 |
US5175109A (en) * | 1986-09-10 | 1992-12-29 | Toa Medical Electronics Co., Ltd. | Reagent for classifying leukocytes by flow cytometry |
US4783401A (en) * | 1986-10-31 | 1988-11-08 | Smithkline Beckman Corporation | Viable cell labelling |
US5179026A (en) * | 1986-11-27 | 1993-01-12 | Toa Medical Electronics Co., Ltd. | Method of classifying leukocytes by flow cytometry and reagents used in the method |
US5039613A (en) * | 1986-11-27 | 1991-08-13 | Toa Medical Electronics Co., Ltd. | Reagents used in a method of classifying leukocytes by flow cytometry |
US4882284A (en) * | 1987-04-13 | 1989-11-21 | Ortho Pharmaceutical Corporation | Method for quantitating and differentiating white blood cells |
JP3038339B2 (ja) * | 1988-05-02 | 2000-05-08 | ザイナクシス・テクノロジーズ・インコーポレーテッド | バイオ粒子の表面膜に対してバイオアフェクティング物質を結合する化合物 |
US5407794A (en) * | 1992-09-08 | 1995-04-18 | Cytocolor Inc. | Oxazine stained lymphocytes and method |
DE4309328C2 (de) * | 1993-03-18 | 1998-03-12 | Volker Ost | Verfahren zur Differenzierung, Konzentrationsbestimmung und Sortierung von Erythrozyten, Thrombozyten und Leukozyten |
US5352613A (en) * | 1993-10-07 | 1994-10-04 | Tafas Triantafillos P | Cytological screening method |
US6136540A (en) * | 1994-10-03 | 2000-10-24 | Ikonisys Inc. | Automated fluorescence in situ hybridization detection of genetic abnormalities |
AU701948B2 (en) * | 1994-10-20 | 1999-02-11 | Sysmex Corporation | Reagent for analyzing solid components in urine and method for analyzing solid components by employing the same |
US6235536B1 (en) * | 1998-03-07 | 2001-05-22 | Robert A. Levine | Analysis of quiescent anticoagulated whole blood samples |
US6197593B1 (en) | 1998-10-20 | 2001-03-06 | Coulter International Corp. | Method for enumerating blood cells |
CA2441878A1 (en) * | 2001-03-19 | 2002-09-26 | Ikonisys, Inc. | System and method for increasing the contrast of an image produced by an epifluorescence microscope |
WO2008124589A2 (en) | 2007-04-06 | 2008-10-16 | California Institute Of Technology | Microfluidic device |
EP2331992A4 (en) | 2008-10-02 | 2014-12-17 | Pixcell Medical Technologies Ltd | VISCOELASTIC FOCUSING BASED OPTICAL IMAGING |
EP2450706B1 (en) * | 2009-07-03 | 2017-07-26 | Sysmex Corporation | Blood analyzer and blood analyzing method |
WO2013082029A1 (en) * | 2011-11-28 | 2013-06-06 | California Institute Of Technology | Compositions and methods for leukocyte differential counting |
US10610861B2 (en) | 2012-12-17 | 2020-04-07 | Accellix Ltd. | Systems, compositions and methods for detecting a biological condition |
US20140170678A1 (en) | 2012-12-17 | 2014-06-19 | Leukodx Ltd. | Kits, compositions and methods for detecting a biological condition |
EP3508848B1 (en) | 2012-12-17 | 2022-10-05 | Accellix Ltd | Systems and methods for determining a chemical state |
Family Cites Families (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
BE403179A (it) * | 1933-05-16 | |||
JPS5239055B1 (it) * | 1966-05-24 | 1977-10-03 | ||
US3617185A (en) * | 1968-08-19 | 1971-11-02 | Gaf Corp | Stable highly concentrated solutions of basic dyes |
DE2153473A1 (de) * | 1971-10-27 | 1973-05-03 | Reflecta G Junge | Filmleuchte |
US3883247A (en) * | 1973-10-30 | 1975-05-13 | Bio Physics Systems Inc | Method for fluorescence analysis of white blood cells |
US3985500A (en) * | 1974-08-21 | 1976-10-12 | Gaf Corporation | Black dye formulation |
DE2515966C3 (de) * | 1975-04-11 | 1979-11-22 | Boehringer Mannheim Gmbh, 6800 Mannheim | Vorgefärbte Objektträger für die Blutunte rsuchung |
FR2315251A1 (fr) * | 1975-06-27 | 1977-01-21 | Inst Nat Sante Rech Med | Procede et composition metachromatique pour la numeration des leucocytes, plus particulierement des basophiles |
DE2962866D1 (en) * | 1978-03-09 | 1982-07-08 | Merck Patent Gmbh | Method for determining leukaemic cells |
US4581223A (en) * | 1980-03-12 | 1986-04-08 | Lawrence Kass | Individual leukocyte determination by means of differential metachromatic dye sorption |
-
1981
- 1981-03-11 US US06/242,662 patent/US4400370A/en not_active Expired - Lifetime
-
1982
- 1982-03-01 CA CA000397322A patent/CA1191079A/en not_active Expired
- 1982-03-02 GB GB8206009A patent/GB2095402B/en not_active Expired
- 1982-03-09 IT IT47950/82A patent/IT1148123B/it active
- 1982-03-10 DE DE19823208629 patent/DE3208629A1/de active Granted
- 1982-03-10 FR FR8204053A patent/FR2501865B2/fr not_active Expired
- 1982-03-10 JP JP57036709A patent/JPS57161550A/ja active Granted
Also Published As
Publication number | Publication date |
---|---|
GB2095402B (en) | 1984-10-10 |
JPH0415416B2 (it) | 1992-03-17 |
FR2501865A2 (fr) | 1982-09-17 |
IT1148123B (it) | 1986-11-26 |
DE3208629A1 (de) | 1982-09-23 |
CA1191079A (en) | 1985-07-30 |
JPS57161550A (en) | 1982-10-05 |
FR2501865B2 (fr) | 1985-11-15 |
DE3208629C2 (it) | 1992-06-11 |
GB2095402A (en) | 1982-09-29 |
US4400370A (en) | 1983-08-23 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
TA | Fee payment date (situation as of event date), data collected since 19931001 |
Effective date: 19990331 |