FR2523310B2 - Procede d'analyse et de determination, par sorption differentielle d'un colorant metachromatique et emission d'une lumiere fluorescente, des divers leucocytes du sang a leurs divers stades de developpement - Google Patents

Procede d'analyse et de determination, par sorption differentielle d'un colorant metachromatique et emission d'une lumiere fluorescente, des divers leucocytes du sang a leurs divers stades de developpement

Info

Publication number
FR2523310B2
FR2523310B2 FR8302264A FR8302264A FR2523310B2 FR 2523310 B2 FR2523310 B2 FR 2523310B2 FR 8302264 A FR8302264 A FR 8302264A FR 8302264 A FR8302264 A FR 8302264A FR 2523310 B2 FR2523310 B2 FR 2523310B2
Authority
FR
France
Prior art keywords
various
emission
determination
analysis
fluorescent light
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
FR8302264A
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English (en)
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FR2523310A2 (fr
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Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of FR2523310A2 publication Critical patent/FR2523310A2/fr
Application granted granted Critical
Publication of FR2523310B2 publication Critical patent/FR2523310B2/fr
Expired legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G01N21/6458—Fluorescence microscopy
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5094—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for blood cell populations
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • G01N2001/305—Fixative compositions

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Engineering & Computer Science (AREA)
  • Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Biomedical Technology (AREA)
  • Cell Biology (AREA)
  • Urology & Nephrology (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Microbiology (AREA)
  • Optics & Photonics (AREA)
  • Ecology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
FR8302264A 1982-03-09 1983-02-11 Procede d'analyse et de determination, par sorption differentielle d'un colorant metachromatique et emission d'une lumiere fluorescente, des divers leucocytes du sang a leurs divers stades de developpement Expired FR2523310B2 (fr)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US06/356,578 US4500509A (en) 1981-03-11 1982-03-09 Metachromatic dye sorption and fluorescent light emmisive means for differential determination of developmental stages of neutrophilic granulocytic cells and other leukocytes

Publications (2)

Publication Number Publication Date
FR2523310A2 FR2523310A2 (fr) 1983-09-16
FR2523310B2 true FR2523310B2 (fr) 1986-12-19

Family

ID=23402036

Family Applications (1)

Application Number Title Priority Date Filing Date
FR8302264A Expired FR2523310B2 (fr) 1982-03-09 1983-02-11 Procede d'analyse et de determination, par sorption differentielle d'un colorant metachromatique et emission d'une lumiere fluorescente, des divers leucocytes du sang a leurs divers stades de developpement

Country Status (7)

Country Link
US (1) US4500509A (fr)
JP (1) JPS58166261A (fr)
CA (1) CA1205365A (fr)
DE (1) DE3304795A1 (fr)
FR (1) FR2523310B2 (fr)
GB (1) GB2116712B (fr)
IT (1) IT1167077B (fr)

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4615878A (en) * 1980-03-12 1986-10-07 Lawrence Kass Metachromatic dye sorption means for differential determination of sub-populations of lymphocytes
EP0183716B1 (fr) * 1984-04-27 1989-01-04 Lawrence Kass Identification de myeloblastes et d'autres cellules granulocytiques immatures
US4714606A (en) * 1984-05-15 1987-12-22 Cytocolor Incorporated Method of staining and identifying cells and compositions thereof
WO1985005446A1 (fr) * 1984-05-15 1985-12-05 Merck Patent Gmbh Colorant unique de remplacement de compositions variables de romanowsky a colorant multiple pour identifications des composants d'echantillons de biopsie humaine
US4751179A (en) * 1984-05-31 1988-06-14 Coulter Electronics, Inc. Method and reagents for differential determination of four populations of leukocytes in blood
US4622291A (en) * 1984-06-11 1986-11-11 The United States Of America As Represented By The Department Of Health And Human Services Method and device for quantitative end point determination in immunofluorescence using microfluorophotometry
WO1986001293A1 (fr) * 1984-08-09 1986-02-27 Lawrence Kass Differenciation, identification et enumeration de neutrophiles, d'erythroblastes et de sous-populations de lymphocytes avec du bleu basique 141
US4707451A (en) * 1985-09-18 1987-11-17 Becton, Dickinson And Company Detection of reticulocytes
US4810487A (en) * 1986-01-02 1989-03-07 Cytocolor, Inc. Method of identifying polymethine stained lymphocyte subpopulations and compositions thereof
EP0430968B1 (fr) * 1988-05-02 1996-11-20 PHANOS TECHNOLOGIES, Inc. Composes, compositions et procede de liaison de substances de bio-affection sur des membranes superficielles de bio-particules
JP2683848B2 (ja) * 1991-02-15 1997-12-03 日本光電工業株式会社 血液モデル
US5407794A (en) * 1992-09-08 1995-04-18 Cytocolor Inc. Oxazine stained lymphocytes and method
DE4309328C2 (de) * 1993-03-18 1998-03-12 Volker Ost Verfahren zur Differenzierung, Konzentrationsbestimmung und Sortierung von Erythrozyten, Thrombozyten und Leukozyten
US5843644A (en) * 1994-03-01 1998-12-01 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Isolation of cellular material under microscopic visualization using an adhesive/extraction reagent tipped probe
US6251467B1 (en) 1994-03-01 2001-06-26 The United States Of America As Represented By The Department Of Health And Human Services Isolation of cellular material under microscopic visualization
US5843657A (en) 1994-03-01 1998-12-01 The United States Of America As Represented By The Department Of Health And Human Services Isolation of cellular material under microscopic visualization
DE19503434A1 (de) * 1995-02-03 1996-08-08 Beiersdorf Ag Verfahren zur Bestimmung der Adhäsion von Mikroorganismen und der antiadhäsiven Wirkung von Stoffen
WO1998045413A1 (fr) * 1997-04-08 1998-10-15 Pall Corporation Procede de prelevement de cellules rares dans des produits sanguins
US6567214B2 (en) * 1997-09-04 2003-05-20 Andrew E. Lorincz Microscope slide having culture media and method for use
DE10124340A1 (de) 2001-05-18 2002-12-05 Fraunhofer Ges Forschung Verfahren zur Analyse einer biologischen Probe
JP2011516833A (ja) * 2008-03-21 2011-05-26 アボット・ポイント・オブ・ケア 蛍光消光及び／又は蛍光退色を用いて個々の細胞又は粒状物質を分析するための方法及び装置
CN105051538B (zh) 2012-12-17 2018-06-15 伦珂德克斯有限公司 用于检测生物状况的系统和方法

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BE403179A (fr) * 1933-05-16
US3327119A (en) * 1964-03-26 1967-06-20 Ibm Method and apparatus for detecting cancer cells
JPS5239055B1 (fr) * 1966-05-24 1977-10-03
US3617185A (en) * 1968-08-19 1971-11-02 Gaf Corp Stable highly concentrated solutions of basic dyes
US3684377A (en) * 1970-07-13 1972-08-15 Bio Physics Systems Inc Method for analysis of blood by optical analysis of living cells
US3916205A (en) * 1973-05-31 1975-10-28 Block Engineering Differential counting of leukocytes and other cells
US4146604A (en) * 1973-05-31 1979-03-27 Block Engineering, Inc. Differential counting of leukocytes and other cells
CA1024048A (fr) * 1973-10-29 1978-01-10 Richard Sternheimer Colorant du sediment urinaire
US3883247A (en) * 1973-10-30 1975-05-13 Bio Physics Systems Inc Method for fluorescence analysis of white blood cells
US3916197A (en) * 1973-11-28 1975-10-28 Particle Technology Inc Method and apparatus for classifying biological cells
US3899297A (en) * 1973-12-19 1975-08-12 Block Engineering Biological staining technique and mixture thereof
US3985500A (en) * 1974-08-21 1976-10-12 Gaf Corporation Black dye formulation
DE2515966C3 (de) * 1975-04-11 1979-11-22 Boehringer Mannheim Gmbh, 6800 Mannheim Vorgefärbte Objektträger für die Blutunte rsuchung
EP0004061B1 (fr) * 1978-03-09 1982-05-19 MERCK PATENT GmbH Procédé pour la détermination de cellules leucémiques
US4581223A (en) * 1980-03-12 1986-04-08 Lawrence Kass Individual leukocyte determination by means of differential metachromatic dye sorption
US4400370A (en) * 1980-03-12 1983-08-23 Lawrence Kass Metachromatic dye sorption means for differential determination of leukocytes
CA1155041A (fr) * 1980-04-21 1983-10-11 Michael E. Jolley Colorants fluorescents des acides nucleiques

Also Published As

Publication number Publication date
GB2116712B (en) 1985-07-10
IT1167077B (it) 1987-05-06
GB8303897D0 (en) 1983-03-16
US4500509A (en) 1985-02-19
IT8347700A0 (it) 1983-02-11
DE3304795C2 (fr) 1992-06-04
GB2116712A (en) 1983-09-28
FR2523310A2 (fr) 1983-09-16
JPH0473104B2 (fr) 1992-11-19
CA1205365A (fr) 1986-06-03
JPS58166261A (ja) 1983-10-01
DE3304795A1 (de) 1983-09-22

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