IN2014MN01822A - - Google Patents

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Publication number
IN2014MN01822A
IN2014MN01822A IN1822MUN2014A IN2014MN01822A IN 2014MN01822 A IN2014MN01822 A IN 2014MN01822A IN 1822MUN2014 A IN1822MUN2014 A IN 1822MUN2014A IN 2014MN01822 A IN2014MN01822 A IN 2014MN01822A
Authority
IN
India
Prior art keywords
endonuclease
sequence
residue
seq
enzymes
Prior art date
Application number
Other languages
English (en)
Inventor
Olav Lanes
Linda Havdalen
Terese Solstad
Marit Lorentzen
Bjørn Altermark
Ingar Leiros
Ronny Helland
Original Assignee
Biotec Pharmacon Asa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GBGB1202768.6A external-priority patent/GB201202768D0/en
Priority claimed from GBGB1216029.7A external-priority patent/GB201216029D0/en
Application filed by Biotec Pharmacon Asa filed Critical Biotec Pharmacon Asa
Publication of IN2014MN01822A publication Critical patent/IN2014MN01822A/en

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/16Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y301/00Hydrolases acting on ester bonds (3.1)
    • C12Y301/21Endodeoxyribonucleases producing 5'-phosphomonoesters (3.1.21)
    • C12Y301/21001Deoxyribonuclease I (3.1.21.1)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00Reaction characterised by the enzymatic activity
    • C12Q2521/30Phosphoric diester hydrolysing, i.e. nuclease
    • C12Q2521/301Endonuclease

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
IN1822MUN2014 2012-02-17 2013-02-18 IN2014MN01822A (enrdf_load_stackoverflow)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GBGB1202768.6A GB201202768D0 (en) 2012-02-17 2012-02-17 Endonucleases
GBGB1216029.7A GB201216029D0 (en) 2012-09-07 2012-09-07 Endonucleases
PCT/GB2013/050387 WO2013121228A1 (en) 2012-02-17 2013-02-18 Endonucleases

Publications (1)

Publication Number Publication Date
IN2014MN01822A true IN2014MN01822A (enrdf_load_stackoverflow) 2015-07-03

Family

ID=47741183

Family Applications (1)

Application Number Title Priority Date Filing Date
IN1822MUN2014 IN2014MN01822A (enrdf_load_stackoverflow) 2012-02-17 2013-02-18

Country Status (12)

Country Link
US (2) US9422595B2 (enrdf_load_stackoverflow)
EP (1) EP2814954B1 (enrdf_load_stackoverflow)
JP (2) JP6259775B2 (enrdf_load_stackoverflow)
KR (1) KR102111178B1 (enrdf_load_stackoverflow)
CN (1) CN104245928B (enrdf_load_stackoverflow)
AU (1) AU2013220102A1 (enrdf_load_stackoverflow)
CA (1) CA2863756C (enrdf_load_stackoverflow)
DK (1) DK2814954T3 (enrdf_load_stackoverflow)
ES (1) ES2622906T3 (enrdf_load_stackoverflow)
IN (1) IN2014MN01822A (enrdf_load_stackoverflow)
LT (1) LT2814954T (enrdf_load_stackoverflow)
WO (1) WO2013121228A1 (enrdf_load_stackoverflow)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB201414745D0 (en) 2014-08-19 2014-10-01 Articzymes As Exonucleases
US11246905B2 (en) 2016-08-15 2022-02-15 President And Fellows Of Harvard College Treating infections using IdsD from Proteus mirabilis
CN109207563A (zh) * 2017-06-30 2019-01-15 青岛科技大学 一种lamp防污染的方法
US20220251146A1 (en) * 2019-05-06 2022-08-11 President And Fellows Of Harvard College Dna-degrading proteins and uses thereof
CN112176028B (zh) * 2019-07-05 2024-04-16 深圳华大生命科学研究院 一种基于核酸内切酶的快速wgs建库方法
PL431144A1 (pl) * 2019-09-13 2021-03-22 Blirt Spółka Akcyjna Nowa, niespecyficzna termolabilna nukleaza aktywna w niskiej temperaturze, szerokim zakresie pH oraz wysokich stężeniach soli
US20240218438A1 (en) 2021-05-27 2024-07-04 Lumiradx Uk Ltd. Improvements in or relating to digestion of reaction products
JP2025502465A (ja) 2022-01-20 2025-01-24 シー‐レクタ・ゲゼルシャフト・ミット・ベシュレンクテル・ハフツング 改善された塩耐性および/または温度性能を有するヌクレアーゼ
CN119351373A (zh) * 2023-07-24 2025-01-24 北京志道生物科技有限公司 一种核酸内切酶及其应用
CN118165957B (zh) * 2024-03-18 2025-06-13 北京石油化工学院 一种重组核酸酶及其应用
CN120173915B (zh) * 2025-05-21 2025-08-26 翌圣生物科技(上海)股份有限公司 一种全能核酸酶突变体及其应用

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5006472A (en) 1988-06-03 1991-04-09 Miles Inc. Enzymatic purification process
US5700672A (en) 1992-07-23 1997-12-23 Stratagene Purified thermostable pyrococcus furiousus DNA ligase
GB9716664D0 (en) 1997-08-06 1997-10-15 Norwegian Inst Of Fisheries & A method of removing nucleic acid contamination reactions
US20020172972A1 (en) 2001-05-15 2002-11-21 President And Fellows Of Harvard College Use of a selectively inactivatable enzyme to digest contaminating nucleic acid
DE102005009479A1 (de) 2005-03-02 2006-09-07 Molzym Gmbh & Co. Kg Verwendung von Nukleasen zum Abbau von Nukleinsäure in Gegenwart von chaotropen Agenzien und/oder Tensiden
JP4857260B2 (ja) 2005-03-08 2012-01-18 タカラバイオ株式会社 低温性微生物由来エンドヌクレアーゼ
US20080044851A1 (en) 2006-06-02 2008-02-21 Epicentre Technologies Compositions and methods for removal of DNA from a sample
US20090311370A1 (en) 2006-06-08 2009-12-17 Agave Inc. And Universidad Autonoma De Guadalajara Highly water-soluble agave inulin, agave inulin-containing product, agave inulin-origin product, by-product and method for producing the same
US20110195486A1 (en) 2008-05-23 2011-08-11 Life Technologies Corporation Methods for removing nucleic acid contamination from reagents
GB2474225A (en) 2009-07-21 2011-04-13 Biotec Pharmacon Asa DNase for decontamination of reverse transcription and amplification reactions

Also Published As

Publication number Publication date
JP6259775B2 (ja) 2018-01-10
WO2013121228A1 (en) 2013-08-22
KR20140135962A (ko) 2014-11-27
EP2814954A1 (en) 2014-12-24
US9650618B2 (en) 2017-05-16
EP2814954B1 (en) 2017-04-05
US20160355798A1 (en) 2016-12-08
CN104245928B (zh) 2017-08-18
JP2018038414A (ja) 2018-03-15
JP2015507927A (ja) 2015-03-16
DK2814954T3 (en) 2017-05-15
CA2863756A1 (en) 2013-08-22
KR102111178B1 (ko) 2020-05-15
US20140370514A1 (en) 2014-12-18
ES2622906T3 (es) 2017-07-07
CN104245928A (zh) 2014-12-24
CA2863756C (en) 2018-03-06
AU2013220102A1 (en) 2014-08-21
LT2814954T (lt) 2017-05-10
US9422595B2 (en) 2016-08-23

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