IL91762A - Process for re-oxidation of steroids and genetically engineered cells used for this purpose - Google Patents

Process for re-oxidation of steroids and genetically engineered cells used for this purpose

Info

Publication number
IL91762A
IL91762A IL9176289A IL9176289A IL91762A IL 91762 A IL91762 A IL 91762A IL 9176289 A IL9176289 A IL 9176289A IL 9176289 A IL9176289 A IL 9176289A IL 91762 A IL91762 A IL 91762A
Authority
IL
Israel
Prior art keywords
proteins
conversion
recombinant host
cells
oxidation
Prior art date
Application number
IL9176289A
Other languages
English (en)
Hebrew (he)
Other versions
IL91762A0 (en
Original Assignee
Roussel Uclaf
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Roussel Uclaf filed Critical Roussel Uclaf
Publication of IL91762A0 publication Critical patent/IL91762A0/xx
Publication of IL91762A publication Critical patent/IL91762A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y106/00—Oxidoreductases acting on NADH or NADPH (1.6)
    • C12Y106/02—Oxidoreductases acting on NADH or NADPH (1.6) with a heme protein as acceptor (1.6.2)
    • C12Y106/02004—NADPH-hemoprotein reductase (1.6.2.4), i.e. NADP-cytochrome P450-reductase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0036—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on NADH or NADPH (1.6)
    • C12N9/0038—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on NADH or NADPH (1.6) with a heme protein as acceptor (1.6.2)
    • C12N9/0042—NADPH-cytochrome P450 reductase (1.6.2.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0095—Oxidoreductases (1.) acting on iron-sulfur proteins as donor (1.18)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P33/00—Preparation of steroids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01053—3-Alpha (or 20-beta)-hydroxysteroid dehydrogenase (1.1.1.53)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y114/00—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
    • C12Y114/15—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14) with reduced iron-sulfur protein as one donor, and incorporation of one atom of oxygen (1.14.15)
    • C12Y114/15004—Steroid 11-beta-monooxygenase (1.14.15.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y114/00—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
    • C12Y114/15—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14) with reduced iron-sulfur protein as one donor, and incorporation of one atom of oxygen (1.14.15)
    • C12Y114/15006—Cholesterol monooxygenase (side-chain-cleaving) (1.14.15.6), i.e. cytochrome P450scc
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y114/00—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
    • C12Y114/99—Miscellaneous (1.14.99)
    • C12Y114/99009—Steroid 17-alpha-monooxygenase (1.14.99.9), i.e. cytochrome-P450-steroid-17-alpha-hydroxylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y118/00—Oxidoreductases acting on iron-sulfur proteins as donors (1.18)
    • C12Y118/01—Oxidoreductases acting on iron-sulfur proteins as donors (1.18) with NAD+ or NADP+ as acceptor (1.18.1)
    • C12Y118/01002—Ferredoxin-NADP+ reductase (1.18.1.2)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Steroid Compounds (AREA)
IL9176289A 1988-09-23 1989-09-24 Process for re-oxidation of steroids and genetically engineered cells used for this purpose IL91762A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP88202080 1988-09-23

Publications (2)

Publication Number Publication Date
IL91762A0 IL91762A0 (en) 1990-06-10
IL91762A true IL91762A (en) 1995-03-30

Family

ID=8199859

Family Applications (1)

Application Number Title Priority Date Filing Date
IL9176289A IL91762A (en) 1988-09-23 1989-09-24 Process for re-oxidation of steroids and genetically engineered cells used for this purpose

Country Status (14)

Country Link
EP (1) EP0360361A1 (fi)
JP (1) JPH03501445A (fi)
KR (1) KR900702029A (fi)
CN (1) CN1042567A (fi)
AU (1) AU638218B2 (fi)
CA (1) CA1340563C (fi)
DK (1) DK175631B1 (fi)
FI (1) FI101551B1 (fi)
HU (1) HU218111B (fi)
IE (1) IE71166B1 (fi)
IL (1) IL91762A (fi)
NZ (1) NZ230761A (fi)
PT (1) PT92543B (fi)
WO (1) WO1990003428A1 (fi)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5420027A (en) * 1991-01-10 1995-05-30 Board Of Regents, The University Of Texas System Methods and compositions for the expression of biologically active fusion proteins comprising a eukaryotic cytochrome P450 fused to a reductase in bacteria
US5240831A (en) * 1991-01-10 1993-08-31 Board Of Regents, The University Of Texas Methods and compositions for the expression of biologically active eukaryotic cytochrome p45os in bacteria
WO1993008260A1 (en) * 1991-10-22 1993-04-29 Board Of Regents, The University Of Texas System Yeasts containing multiple mammalian cytochromes for degrading complex chemical mixtures
US5705400A (en) * 1992-03-07 1998-01-06 Rsr Limited Assay for adrenal autoantigen
GB2256046A (en) * 1992-07-31 1992-11-25 R S R Limited Assay for adrenal autoantibodies useful in the diagnosis of addison's disease
IL116872A (en) * 1995-02-15 2004-12-15 Aventis Pharma Sa Delta 5,7-Strol Reductase from Arbidopsis Thaliana
FR2812884B1 (fr) 2000-08-08 2002-11-08 Aventis Pharma Sa Levures modifiees et utilisations, notamment pour la production de derives steroidiens
FR2820145B1 (fr) * 2001-01-31 2004-01-23 Aventis Pharma Sa Souche de levure produisant des steroides de facon autonome
FR2976949B1 (fr) * 2011-06-21 2015-08-28 Sanofi Sa Procede de preparation de levures genetiquement transformees capables de produire une molecule d'interet a haut titre
WO2024131933A1 (en) * 2022-12-23 2024-06-27 Hangzhou Enhe Biotechnology Co., Ltd. Engineered microbes for production of progesterone and ursodeoxycholic acid by fermentation

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4720454A (en) * 1984-04-18 1988-01-19 White Perrin C Genetic probe used in the detection of adrenal hyperplasia
PT90484B (pt) * 1988-05-06 1994-08-31 Roussel Uclaf Processo para a oxidacao bioquimica de esteroides e para a preparacao por engenharia genetica de celulas para esse fim

Also Published As

Publication number Publication date
IL91762A0 (en) 1990-06-10
AU638218B2 (en) 1993-06-24
FI101551B (fi) 1998-07-15
FI101551B1 (fi) 1998-07-15
IE71166B1 (en) 1997-01-29
DK108790D0 (da) 1990-05-02
WO1990003428A1 (en) 1990-04-05
PT92543B (pt) 1996-10-31
EP0360361A1 (en) 1990-03-28
PT92543A (pt) 1990-03-30
FI902024A0 (fi) 1990-04-23
AU4428289A (en) 1990-04-18
DK175631B1 (da) 2004-12-27
CN1042567A (zh) 1990-05-30
JPH03501445A (ja) 1991-04-04
KR900702029A (ko) 1990-12-05
IE893054L (en) 1990-03-23
NZ230761A (en) 1992-06-25
HUT53393A (en) 1990-10-28
CA1340563C (en) 1999-05-25
DK108790A (da) 1990-05-02
HU218111B (hu) 2000-06-28

Similar Documents

Publication Publication Date Title
Szczebara et al. Total biosynthesis of hydrocortisone from a simple carbon source in yeast
Duport et al. Self-sufficient biosynthesis of pregnenolone and progesterone in engineered yeast
WO2010079594A1 (ja) ステロール側鎖切断酵素蛋白質およびその利用
MXPA03006031A (es) Cepas de levaduras que producen esteroides de manera autonoma.
AU638218B2 (en) Process for the multiple oxidation of steroids and genetically engineered cells to be used therefor
CA1340616C (en) Process for the biochemical oxidation of steroids and genetically engineered cells to be used therefor
Karpov et al. Creation and functional analysis of Mycolicibacterium smegmatis recombinant strains carrying the bacillary cytochromes CYP106A1 and CYP106A2 genes
EP0340878B1 (en) Process for the biochemical oxidation of steroids and genetically engineered cells to be used therefor
CN115029368B (zh) 一种产双降醇的基因工程菌及其用途
US5869283A (en) Expression cassette operable in a recombinant host
JP2001524838A (ja) 中断されたａｔｆ２遺伝子を有する酵母株及びそれらの利用
Malik Aspects of biotechnology in steriod biotransformation
IE83307B1 (en) Process for the biochemical oxidation of steroids and genetically engineered cells to be used therefor
JPH0569511B2 (fi)
JP3577722B2 (ja) 人工融合酵素
US20030108982A1 (en) Process for oxidation of steroids and genetically engineered cells used therein
JPH07147975A (ja) 人工融合酵素
US20040067579A1 (en) Process for oxidation of steroids and genetically engineered cells used therein

Legal Events

Date Code Title Description
RH Patent void
HCE Change of name and address of proprietor(s)
KB Patent renewed
MM9K Patent not in force due to non-payment of renewal fees