IL60714A - Process for the preparation of e.coli phoa mutants and plasmids coding for alkaline phosphatase and products thereof - Google Patents
Process for the preparation of e.coli phoa mutants and plasmids coding for alkaline phosphatase and products thereofInfo
- Publication number
- IL60714A IL60714A IL60714A IL6071480A IL60714A IL 60714 A IL60714 A IL 60714A IL 60714 A IL60714 A IL 60714A IL 6071480 A IL6071480 A IL 6071480A IL 60714 A IL60714 A IL 60714A
- Authority
- IL
- Israel
- Prior art keywords
- plasmids
- alkaline phosphatase
- coli
- phoa
- products
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/034—Fusion polypeptide containing a localisation/targetting motif containing a motif for targeting to the periplasmic space of Gram negative bacteria as a soluble protein, i.e. signal sequence should be cleaved
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/185—Escherichia
- C12R2001/19—Escherichia coli
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/425—Serratia
- C12R2001/43—Serratia marcescens
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/82—Subcellular parts of microorganisms
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/8215—Microorganisms
- Y10S435/822—Microorganisms using bacteria or actinomycetales
- Y10S435/848—Escherichia
- Y10S435/849—Escherichia coli
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/8215—Microorganisms
- Y10S435/822—Microorganisms using bacteria or actinomycetales
- Y10S435/88—Serratia
- Y10S435/881—Serratia marcescens
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Molecular Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE19792931999 DE2931999A1 (de) | 1979-08-03 | 1979-08-03 | Herstellung und anwendung von neukombinierten plasmiden mit genen fuer alkalische phosphatasen |
Publications (1)
Publication Number | Publication Date |
---|---|
IL60714A true IL60714A (en) | 1983-12-30 |
Family
ID=6077852
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
IL60714A IL60714A (en) | 1979-08-03 | 1980-07-31 | Process for the preparation of e.coli phoa mutants and plasmids coding for alkaline phosphatase and products thereof |
Country Status (8)
Country | Link |
---|---|
US (1) | US4375514A (da) |
EP (1) | EP0023882B1 (da) |
JP (2) | JPS5648884A (da) |
AT (1) | ATE32101T1 (da) |
DE (2) | DE2931999A1 (da) |
DK (1) | DK329480A (da) |
HU (1) | HU193504B (da) |
IL (1) | IL60714A (da) |
Families Citing this family (26)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DK582381A (da) * | 1981-01-02 | 1982-07-03 | Univ New York | Remkombinat plasmid til anvendelse ved bakteriel kloning |
JPS5869897A (ja) * | 1981-10-20 | 1983-04-26 | Gakuzo Tamura | Dna遺伝子 |
US4532207A (en) * | 1982-03-19 | 1985-07-30 | G. D. Searle & Co. | Process for the preparation of polypeptides utilizing a charged amino acid polymer and exopeptidase |
US4880911A (en) * | 1982-03-19 | 1989-11-14 | G. D. Searle & Co. | Fused polypeptides and methods for their detection |
US5252482A (en) * | 1982-05-20 | 1993-10-12 | Suntory Limited | Precursor of a C-terminal amidated calcitonin |
JPS60145685A (ja) | 1984-01-09 | 1985-08-01 | Nec Corp | 分布帰還型半導体レ−ザ |
JPS592689A (ja) * | 1982-06-04 | 1984-01-09 | Handai Biseibutsubiyou Kenkyukai | 強力な遺伝子発現能を有する新規レプリコンの作成法 |
JPS5939899A (ja) * | 1982-08-27 | 1984-03-05 | Gakuzo Tamura | 新規ベクタ− |
JPS59198979A (ja) * | 1983-04-28 | 1984-11-10 | Agency Of Ind Science & Technol | 特定プラスミドを含む宿主菌体の安定化 |
US4963495A (en) * | 1984-10-05 | 1990-10-16 | Genentech, Inc. | Secretion of heterologous proteins |
FR2575760A1 (fr) * | 1985-01-04 | 1986-07-11 | Merieux Inst | Nouvelles souches d'escherichia coli excretrices de phosphatase alcaline, procede d'obtention de ces souches, procede de production de phosphatase alcaline et de proteines hybrides derivees |
US5047334A (en) * | 1987-06-30 | 1991-09-10 | Repligen Corporation | Novel prokaryotic expression and secretion system |
DE3731875A1 (de) * | 1987-09-18 | 1989-03-30 | Schering Ag | Gentechnische herstellung von neukombinierten plasmiden fuer die produktion von humanem adrenocorticotropen horman (acth) und davon abgeleiteten derivaten durch enzymatische spaltung primaer synthetisierter fusionsproteine |
US5081227A (en) * | 1988-03-04 | 1992-01-14 | La Jolla Cancer Research Foundation | Germ cell alkaline phosphatase |
WO1989008254A1 (en) * | 1988-03-04 | 1989-09-08 | La Jolla Cancer Research Foundation | Germ cell alkaline phosphatase gene and protein produced thereby |
FR2648713B1 (fr) * | 1989-06-26 | 1994-06-10 | Commissariat Energie Atomique | Proteines hybrides entre une enzyme extracytoplasmique et une autre proteine, leur procede de preparation, ainsi que leur application comme agent de diagnostic |
US6451572B1 (en) | 1998-06-25 | 2002-09-17 | Cornell Research Foundation, Inc. | Overexpression of phytase genes in yeast systems |
ES2248067T3 (es) | 1999-03-31 | 2006-03-16 | Cornell Research Foundation, Inc. | Fosfatasas con actividad de fitasa mejorada. |
US6841370B1 (en) | 1999-11-18 | 2005-01-11 | Cornell Research Foundation, Inc. | Site-directed mutagenesis of Escherichia coli phytase |
EP2335501B8 (en) * | 2001-10-31 | 2016-09-21 | Huvepharma Eood | Phytase-containing animal food and method |
EP1604008B1 (en) * | 2002-09-13 | 2010-12-22 | Cornell Research Foundation, Inc. | Using mutations to improve aspergillus phytases |
US7919297B2 (en) | 2006-02-21 | 2011-04-05 | Cornell Research Foundation, Inc. | Mutants of Aspergillus niger PhyA phytase and Aspergillus fumigatus phytase |
EP2069486A2 (en) * | 2006-08-03 | 2009-06-17 | Cornell Research Foundation, Inc. | Phytases with improved thermal stability |
US20080239709A1 (en) * | 2007-03-29 | 2008-10-02 | Thomas & Betts International, Inc. | Concealed Emergency Lighting Fixture with Full Rotation of Door |
US8192734B2 (en) | 2007-07-09 | 2012-06-05 | Cornell University | Compositions and methods for bone strengthening |
JP6893000B2 (ja) * | 2018-08-17 | 2021-06-23 | ボンタック バイオエンジニアリング(シェンゼン) カンパニー リミテッド | 酸性ホスファターゼ突然変異体及びニコチンアミドリボシドの調製方法 |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4237224A (en) * | 1974-11-04 | 1980-12-02 | Board Of Trustees Of The Leland Stanford Jr. University | Process for producing biologically functional molecular chimeras |
US4190495A (en) * | 1976-09-27 | 1980-02-26 | Research Corporation | Modified microorganisms and method of preparing and using same |
-
1979
- 1979-08-03 DE DE19792931999 patent/DE2931999A1/de not_active Ceased
-
1980
- 1980-07-28 DE DE8080730051T patent/DE3072070D1/de not_active Expired
- 1980-07-28 AT AT80730051T patent/ATE32101T1/de not_active IP Right Cessation
- 1980-07-28 EP EP80730051A patent/EP0023882B1/de not_active Expired
- 1980-07-31 DK DK329480A patent/DK329480A/da not_active Application Discontinuation
- 1980-07-31 IL IL60714A patent/IL60714A/xx unknown
- 1980-08-01 US US06/174,316 patent/US4375514A/en not_active Expired - Lifetime
- 1980-08-01 HU HU801932A patent/HU193504B/hu unknown
- 1980-08-04 JP JP10647980A patent/JPS5648884A/ja active Pending
-
1986
- 1986-04-24 JP JP61093478A patent/JPS6229966A/ja active Pending
Also Published As
Publication number | Publication date |
---|---|
DE3072070D1 (en) | 1988-02-25 |
EP0023882A2 (de) | 1981-02-11 |
EP0023882A3 (en) | 1981-12-09 |
ATE32101T1 (de) | 1988-02-15 |
DK329480A (da) | 1981-02-04 |
EP0023882B1 (de) | 1988-01-20 |
HU193504B (en) | 1987-10-28 |
JPS5648884A (da) | 1981-05-02 |
JPS6229966A (ja) | 1987-02-07 |
DE2931999A1 (de) | 1981-02-26 |
US4375514A (en) | 1983-03-01 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
IL60714A (en) | Process for the preparation of e.coli phoa mutants and plasmids coding for alkaline phosphatase and products thereof | |
Wolfe et al. | The isolation of homogeneous leader peptidase from a strain of Escherichia coli which overproduces the enzyme. | |
EP0799317B8 (en) | Method for secreting thrombopoietin polypeptides | |
GB2007676A (en) | Method and means for microbial polypeptide expression | |
BG60107B2 (bg) | Метод за получаване на еукариотен полипептид | |
AU4067985A (en) | Hosts and methods for producing recombinant products in high yields | |
EP0120551A3 (en) | Yeast expression systems with vectors having gapdh or pyk promoters, and synthesis of foreign proteins | |
Chen et al. | Construction of leaky strains and extracellular production of exogenous proteins in recombinant E scherichia coli | |
NO910797D0 (no) | Dna-fragment som koder for et polypeptid med nitrilhydratase-aktivitet, en transformant inneholdende genet og en fremgangsmaate for fremstilling av amider ved bruk av transformanten. | |
ES8306324A1 (es) | "proceso para la produccion microbiana de interferon fibroblasto humano". | |
UA27706C2 (uk) | Спосіб одержання гетерологічного поліпептиду | |
ES8202586A1 (es) | Un procedimiento para clonar una secuencia de desoxinucleo- tidos | |
Vasquez et al. | An expression system for trypsin | |
AU6569386A (en) | Eukaryotic fusion proteins, the preparation and use thereof and means for carrying out the process | |
EP0068693A3 (en) | Production of foot and mouth disease vaccine from microbially expressed antigens | |
ES2000278A6 (es) | Procedimiento para preparar una proteina de fusion y para preparar una proteina eucariotica. | |
ES548998A0 (es) | Un procedimiento para producir un polipeptido | |
ES8800344A1 (es) | Un metodo de produccion de actividad de isopenicilina n sintetasa en una celula huesped | |
KR950014309A (ko) | 진균성 프로테아제(Fungal protease) | |
KR840008697A (ko) | 상동 효모 신호를 이용한 이종단백질의 합성, 분비 및 표현매개체 제조방법 | |
Vasantha et al. | Secretion of a heterologous protein from Bacillus subtilis with the aid of protease signal sequences | |
IE57651B1 (en) | Expression plasmids for improved production of heterologous protein in bacteria | |
JPH074254B2 (ja) | ヒト成長ホルモンの製法 | |
WO1990014426A3 (en) | Saf polypeptide, gene, promoter and use for expression in streptomyces | |
Haezebrouck et al. | Stability effects associated with the introduction of a partial and a complete Ca2+-binding site into human lysozyme |