IL39063A - Water-insoluble enzyme products - Google Patents
Water-insoluble enzyme productsInfo
- Publication number
- IL39063A IL39063A IL39063A IL3906372A IL39063A IL 39063 A IL39063 A IL 39063A IL 39063 A IL39063 A IL 39063A IL 3906372 A IL3906372 A IL 3906372A IL 39063 A IL39063 A IL 39063A
- Authority
- IL
- Israel
- Prior art keywords
- enzyme
- insoluble
- product
- water
- process according
- Prior art date
Links
- 108090000790 Enzymes Proteins 0.000 title claims description 54
- 102000004190 Enzymes Human genes 0.000 title claims description 54
- 229940088598 enzyme Drugs 0.000 claims description 53
- 238000000034 method Methods 0.000 claims description 15
- 150000004676 glycans Chemical class 0.000 claims description 14
- 229920001282 polysaccharide Polymers 0.000 claims description 14
- 239000005017 polysaccharide Substances 0.000 claims description 14
- 238000006243 chemical reaction Methods 0.000 claims description 10
- 125000003172 aldehyde group Chemical group 0.000 claims description 9
- 239000007853 buffer solution Substances 0.000 claims description 8
- 239000001913 cellulose Substances 0.000 claims description 7
- 229920002678 cellulose Polymers 0.000 claims description 7
- 102000004142 Trypsin Human genes 0.000 claims description 4
- 108090000631 Trypsin Proteins 0.000 claims description 4
- 239000012588 trypsin Substances 0.000 claims description 4
- 108090000526 Papain Proteins 0.000 claims description 3
- 239000004365 Protease Substances 0.000 claims description 3
- 229940055729 papain Drugs 0.000 claims description 3
- 235000019834 papain Nutrition 0.000 claims description 3
- 125000003277 amino group Chemical group 0.000 claims description 2
- 238000004519 manufacturing process Methods 0.000 claims 2
- 239000000047 product Substances 0.000 description 21
- 230000000694 effects Effects 0.000 description 9
- 239000000243 solution Substances 0.000 description 6
- 239000000126 substance Substances 0.000 description 6
- 239000000758 substrate Substances 0.000 description 5
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 4
- 230000015572 biosynthetic process Effects 0.000 description 4
- 238000005119 centrifugation Methods 0.000 description 4
- 230000003647 oxidation Effects 0.000 description 4
- 238000007254 oxidation reaction Methods 0.000 description 4
- 238000000926 separation method Methods 0.000 description 4
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 description 3
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 3
- 238000001914 filtration Methods 0.000 description 3
- 239000011159 matrix material Substances 0.000 description 3
- 239000002609 medium Substances 0.000 description 3
- 229920000642 polymer Polymers 0.000 description 3
- 239000012429 reaction media Substances 0.000 description 3
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 239000001110 calcium chloride Substances 0.000 description 2
- 229910001628 calcium chloride Inorganic materials 0.000 description 2
- 238000004132 cross linking Methods 0.000 description 2
- 230000002255 enzymatic effect Effects 0.000 description 2
- 238000006911 enzymatic reaction Methods 0.000 description 2
- 125000002791 glucosyl group Chemical group C1([C@H](O)[C@@H](O)[C@H](O)[C@H](O1)CO)* 0.000 description 2
- 230000002779 inactivation Effects 0.000 description 2
- FYFFGSSZFBZTAH-UHFFFAOYSA-N methylaminomethanetriol Chemical compound CNC(O)(O)O FYFFGSSZFBZTAH-UHFFFAOYSA-N 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 229910052757 nitrogen Inorganic materials 0.000 description 2
- KHIWWQKSHDUIBK-UHFFFAOYSA-N periodic acid Chemical compound OI(=O)(=O)=O KHIWWQKSHDUIBK-UHFFFAOYSA-N 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 239000000376 reactant Substances 0.000 description 2
- JQWHASGSAFIOCM-UHFFFAOYSA-M sodium periodate Chemical compound [Na+].[O-]I(=O)(=O)=O JQWHASGSAFIOCM-UHFFFAOYSA-M 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- LWIHDJKSTIGBAC-UHFFFAOYSA-K tripotassium phosphate Chemical compound [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- QIJRTFXNRTXDIP-UHFFFAOYSA-N (1-carboxy-2-sulfanylethyl)azanium;chloride;hydrate Chemical compound O.Cl.SCC(N)C(O)=O QIJRTFXNRTXDIP-UHFFFAOYSA-N 0.000 description 1
- TYMLOMAKGOJONV-UHFFFAOYSA-N 4-nitroaniline Chemical compound NC1=CC=C([N+]([O-])=O)C=C1 TYMLOMAKGOJONV-UHFFFAOYSA-N 0.000 description 1
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 1
- QXNVGIXVLWOKEQ-UHFFFAOYSA-N Disodium Chemical class [Na][Na] QXNVGIXVLWOKEQ-UHFFFAOYSA-N 0.000 description 1
- VGGSQFUCUMXWEO-UHFFFAOYSA-N Ethene Chemical compound C=C VGGSQFUCUMXWEO-UHFFFAOYSA-N 0.000 description 1
- 239000005977 Ethylene Substances 0.000 description 1
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 1
- 239000004472 Lysine Substances 0.000 description 1
- 238000000862 absorption spectrum Methods 0.000 description 1
- WTHXTWHYLIZJBH-UHFFFAOYSA-N acetic acid;azane Chemical compound N.CC(O)=O.CC(O)=O.CC(O)=O.CC(O)=O WTHXTWHYLIZJBH-UHFFFAOYSA-N 0.000 description 1
- 125000003158 alcohol group Chemical group 0.000 description 1
- 125000000539 amino acid group Chemical group 0.000 description 1
- 239000012736 aqueous medium Substances 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 239000011324 bead Substances 0.000 description 1
- 230000004397 blinking Effects 0.000 description 1
- 229920001577 copolymer Polymers 0.000 description 1
- 229960001305 cysteine hydrochloride Drugs 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 229960001760 dimethyl sulfoxide Drugs 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 238000005538 encapsulation Methods 0.000 description 1
- 239000008187 granular material Substances 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 229910000160 potassium phosphate Inorganic materials 0.000 description 1
- 235000011009 potassium phosphates Nutrition 0.000 description 1
- 125000006239 protecting group Chemical group 0.000 description 1
- 230000035484 reaction time Effects 0.000 description 1
- RGHFKWPGWBFQLN-UHFFFAOYSA-M sodium;5,5-diethylpyrimidin-3-ide-2,4,6-trione Chemical compound [Na+].CCC1(CC)C([O-])=NC(=O)NC1=O RGHFKWPGWBFQLN-UHFFFAOYSA-M 0.000 description 1
- 235000000346 sugar Nutrition 0.000 description 1
- 150000008163 sugars Chemical class 0.000 description 1
- 238000004448 titration Methods 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 229920003176 water-insoluble polymer Polymers 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
- C12N11/10—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a carbohydrate
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CH468771A CH536860A (fr) | 1971-03-31 | 1971-03-31 | Procédé de préparation d'un produit doué d'activité enzymatique, insoluble en milieu aqueux |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| IL39063A0 IL39063A0 (en) | 1972-05-30 |
| IL39063A true IL39063A (en) | 1974-11-29 |
Family
ID=4280751
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| IL39063A IL39063A (en) | 1971-03-31 | 1972-03-23 | Water-insoluble enzyme products |
Country Status (10)
| Country | Link |
|---|---|
| JP (1) | JPS5622515B1 (enrdf_load_stackoverflow) |
| AT (1) | AT314451B (enrdf_load_stackoverflow) |
| BE (1) | BE780939A (enrdf_load_stackoverflow) |
| CA (1) | CA976098A (enrdf_load_stackoverflow) |
| CH (1) | CH536860A (enrdf_load_stackoverflow) |
| DE (1) | DE2215160C3 (enrdf_load_stackoverflow) |
| DK (1) | DK129460B (enrdf_load_stackoverflow) |
| FR (1) | FR2132080B1 (enrdf_load_stackoverflow) |
| GB (1) | GB1349498A (enrdf_load_stackoverflow) |
| IL (1) | IL39063A (enrdf_load_stackoverflow) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS54113492A (en) * | 1978-02-24 | 1979-09-05 | Sanyo Chem Ind Ltd | Preparation of glucoprotein derivative |
| CS206823B1 (en) * | 1979-04-28 | 1981-07-31 | Karel Filka | Sorbents for saccharides,glycoproteins and polymers,containing saccharides and method of their manufacture |
| DE3033029A1 (de) * | 1979-09-28 | 1981-04-23 | Vsesojuznyj kardiologičeskij naučnyj centr Akademii medicinskich Nauk SSSR,, Moskva | Urokinasederivate und verfahren zu deren herstellung |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DD83154A1 (de) * | 1970-05-22 | 1971-07-12 | Verfahren zur Herstellung wasserunlöslicher Proteine, Proteide und Peptide |
-
1971
- 1971-03-31 CH CH468771A patent/CH536860A/fr not_active IP Right Cessation
-
1972
- 1972-03-20 BE BE780939A patent/BE780939A/xx not_active IP Right Cessation
- 1972-03-23 IL IL39063A patent/IL39063A/en unknown
- 1972-03-24 GB GB1383872A patent/GB1349498A/en not_active Expired
- 1972-03-27 CA CA138,232A patent/CA976098A/en not_active Expired
- 1972-03-27 FR FR7210644A patent/FR2132080B1/fr not_active Expired
- 1972-03-28 DK DK151072AA patent/DK129460B/da not_active IP Right Cessation
- 1972-03-28 DE DE2215160A patent/DE2215160C3/de not_active Expired
- 1972-03-30 AT AT279172A patent/AT314451B/de not_active IP Right Cessation
- 1972-03-31 JP JP3182172A patent/JPS5622515B1/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| DE2215160C3 (de) | 1980-05-08 |
| DE2215160B2 (de) | 1979-08-09 |
| CA976098A (en) | 1975-10-14 |
| FR2132080A1 (enrdf_load_stackoverflow) | 1972-11-17 |
| DK129460B (da) | 1974-10-14 |
| GB1349498A (en) | 1974-04-03 |
| IL39063A0 (en) | 1972-05-30 |
| BE780939A (fr) | 1972-09-20 |
| JPS5622515B1 (enrdf_load_stackoverflow) | 1981-05-26 |
| DK129460C (enrdf_load_stackoverflow) | 1975-04-01 |
| CH536860A (fr) | 1973-05-15 |
| FR2132080B1 (enrdf_load_stackoverflow) | 1976-06-11 |
| DE2215160A1 (de) | 1972-10-12 |
| AT314451B (de) | 1974-04-10 |
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