IL301380A - A multivalent binder for nucleic acid analysis - Google Patents
A multivalent binder for nucleic acid analysisInfo
- Publication number
- IL301380A IL301380A IL301380A IL30138023A IL301380A IL 301380 A IL301380 A IL 301380A IL 301380 A IL301380 A IL 301380A IL 30138023 A IL30138023 A IL 30138023A IL 301380 A IL301380 A IL 301380A
- Authority
- IL
- Israel
- Prior art keywords
- nucleotide
- nucleic acid
- composition
- binding
- polymer
- Prior art date
Links
- 150000007523 nucleic acids Chemical class 0.000 title claims description 402
- 239000000203 mixture Substances 0.000 title claims description 267
- 102000039446 nucleic acids Human genes 0.000 title description 330
- 108020004707 nucleic acids Proteins 0.000 title description 330
- 238000009739 binding Methods 0.000 title description 293
- 230000027455 binding Effects 0.000 title description 292
- 238000004458 analytical method Methods 0.000 title description 15
- 239000002773 nucleotide Substances 0.000 claims description 609
- 125000003729 nucleotide group Chemical group 0.000 claims description 497
- 229920000642 polymer Polymers 0.000 claims description 117
- 238000006243 chemical reaction Methods 0.000 claims description 51
- 238000003384 imaging method Methods 0.000 claims description 50
- 108091028043 Nucleic acid sequence Proteins 0.000 claims description 36
- 239000007787 solid Substances 0.000 claims description 28
- -1 hydroxylamino group Chemical group 0.000 claims description 19
- 239000000412 dendrimer Substances 0.000 claims description 17
- 229920000736 dendritic polymer Polymers 0.000 claims description 17
- 239000012530 fluid Substances 0.000 claims description 15
- 230000003287 optical effect Effects 0.000 claims description 12
- 230000000903 blocking effect Effects 0.000 claims description 11
- 229920006037 cross link polymer Polymers 0.000 claims description 3
- 235000000346 sugar Nutrition 0.000 claims description 3
- 230000008878 coupling Effects 0.000 claims description 2
- 238000010168 coupling process Methods 0.000 claims description 2
- 238000005859 coupling reaction Methods 0.000 claims description 2
- 108091028732 Concatemer Proteins 0.000 claims 1
- 238000010348 incorporation Methods 0.000 description 152
- 238000000034 method Methods 0.000 description 127
- 238000012163 sequencing technique Methods 0.000 description 116
- 238000003199 nucleic acid amplification method Methods 0.000 description 70
- 230000003321 amplification Effects 0.000 description 69
- 239000013615 primer Substances 0.000 description 62
- 230000000295 complement effect Effects 0.000 description 61
- 239000000758 substrate Substances 0.000 description 59
- 239000002777 nucleoside Chemical class 0.000 description 56
- 239000000872 buffer Substances 0.000 description 50
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- 150000003833 nucleoside derivatives Chemical class 0.000 description 44
- 239000002245 particle Substances 0.000 description 44
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- 239000003153 chemical reaction reagent Substances 0.000 description 38
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- 125000005647 linker group Chemical group 0.000 description 32
- 102000053602 DNA Human genes 0.000 description 31
- 108020004414 DNA Proteins 0.000 description 31
- 238000001514 detection method Methods 0.000 description 31
- 210000004027 cell Anatomy 0.000 description 29
- 239000003446 ligand Substances 0.000 description 25
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 24
- 230000005284 excitation Effects 0.000 description 24
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- KHWCHTKSEGGWEX-RRKCRQDMSA-N 2'-deoxyadenosine 5'-monophosphate Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(O)=O)O1 KHWCHTKSEGGWEX-RRKCRQDMSA-N 0.000 description 7
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- XTWYTFMLZFPYCI-KQYNXXCUSA-N 5'-adenylphosphoric acid Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP(O)(=O)OP(O)(O)=O)[C@@H](O)[C@H]1O XTWYTFMLZFPYCI-KQYNXXCUSA-N 0.000 description 7
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- ZKHQWZAMYRWXGA-UHFFFAOYSA-N Adenosine triphosphate Natural products C1=NC=2C(N)=NC=NC=2N1C1OC(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)C(O)C1O ZKHQWZAMYRWXGA-UHFFFAOYSA-N 0.000 description 7
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- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 7
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 7
- AHCYMLUZIRLXAA-SHYZEUOFSA-N Deoxyuridine 5'-triphosphate Chemical compound O1[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)C[C@@H]1N1C(=O)NC(=O)C=C1 AHCYMLUZIRLXAA-SHYZEUOFSA-N 0.000 description 7
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- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 7
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Classifications
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- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
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- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
- C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
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- C12Q2521/00—Reaction characterised by the enzymatic activity
- C12Q2521/10—Nucleotidyl transfering
- C12Q2521/101—DNA polymerase
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
- C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
- C12Q2537/157—A reaction step characterised by the number of molecules incorporated or released
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- C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
- C12Q2563/107—Nucleic acid detection characterized by the use of physical, structural and functional properties fluorescence
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
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Landscapes
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- Chemical & Material Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
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- Genetics & Genomics (AREA)
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
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| US11287422B2 (en) | 2019-09-23 | 2022-03-29 | Element Biosciences, Inc. | Multivalent binding composition for nucleic acid analysis |
| US11408032B2 (en) | 2020-01-17 | 2022-08-09 | Element Biosciences, Inc. | Tube lens design for improved depth-of-field |
| US11198121B1 (en) | 2020-06-10 | 2021-12-14 | Element Biosciences, Inc. | Flow cell systems and devices |
| CN116615560A (zh) | 2020-10-30 | 2023-08-18 | 元素生物科学公司 | 用于大规模平行核酸测序的试剂 |
| KR20230153706A (ko) | 2022-04-29 | 2023-11-07 | 연세대학교 산학협력단 | 핵산의 검출 또는 분리용 조성물 및 이를 이용한 핵산의 검출 또는 분리 방법 |
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| WO2006097320A2 (de) * | 2005-03-17 | 2006-09-21 | Genovoxx Gmbh | Makromolekulare nukleotidverbindungen und methoden zu deren anwendung |
| US20090208957A1 (en) * | 2007-12-04 | 2009-08-20 | Pacific Biosciences Of California, Inc. | Alternate labeling strategies for single molecule sequencing |
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| CN113939601A (zh) | 2022-01-14 |
| WO2020243017A1 (en) | 2020-12-03 |
| EP3947731A1 (de) | 2022-02-09 |
| DE112020002516T5 (de) | 2022-03-24 |
| AU2020285657A1 (en) | 2021-11-18 |
| KR102607124B1 (ko) | 2023-11-29 |
| AU2022291540A1 (en) | 2023-02-02 |
| GB2597398A (en) | 2022-01-26 |
| IL287528A (en) | 2021-12-01 |
| AU2020285657B2 (en) | 2022-10-06 |
| JP2022535187A (ja) | 2022-08-05 |
| IL287528B2 (en) | 2023-08-01 |
| CA3137120A1 (en) | 2020-12-03 |
| GB2597398B (en) | 2024-03-06 |
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