IL296889A - A method for breeding and genealogical description of quantitative skeletal muscle - Google Patents
A method for breeding and genealogical description of quantitative skeletal muscleInfo
- Publication number
- IL296889A IL296889A IL296889A IL29688922A IL296889A IL 296889 A IL296889 A IL 296889A IL 296889 A IL296889 A IL 296889A IL 29688922 A IL29688922 A IL 29688922A IL 296889 A IL296889 A IL 296889A
- Authority
- IL
- Israel
- Prior art keywords
- cell line
- meat product
- differentiation
- myogenic
- cultured meat
- Prior art date
Links
- 238000000034 method Methods 0.000 title claims description 37
- 210000002027 skeletal muscle Anatomy 0.000 title claims description 22
- 210000004027 cell Anatomy 0.000 claims description 147
- 230000004069 differentiation Effects 0.000 claims description 56
- 230000001114 myogenic effect Effects 0.000 claims description 53
- 230000014509 gene expression Effects 0.000 claims description 35
- 108091023040 Transcription factor Proteins 0.000 claims description 29
- 102000040945 Transcription factor Human genes 0.000 claims description 28
- 230000001939 inductive effect Effects 0.000 claims description 21
- 235000013622 meat product Nutrition 0.000 claims description 21
- 210000001519 tissue Anatomy 0.000 claims description 17
- 101000958865 Homo sapiens Myogenic factor 5 Proteins 0.000 claims description 16
- 102100032970 Myogenin Human genes 0.000 claims description 16
- 102100038380 Myogenic factor 5 Human genes 0.000 claims description 15
- 210000000107 myocyte Anatomy 0.000 claims description 15
- 230000001973 epigenetic effect Effects 0.000 claims description 14
- 230000030833 cell death Effects 0.000 claims description 11
- 210000001087 myotubule Anatomy 0.000 claims description 11
- 239000001963 growth medium Substances 0.000 claims description 10
- 210000004263 induced pluripotent stem cell Anatomy 0.000 claims description 9
- 230000004070 myogenic differentiation Effects 0.000 claims description 8
- 230000002068 genetic effect Effects 0.000 claims description 7
- 244000144972 livestock Species 0.000 claims description 7
- 108010077544 Chromatin Proteins 0.000 claims description 6
- 101000613490 Homo sapiens Paired box protein Pax-3 Proteins 0.000 claims description 6
- 102100040891 Paired box protein Pax-3 Human genes 0.000 claims description 6
- 230000004156 Wnt signaling pathway Effects 0.000 claims description 6
- 239000000556 agonist Substances 0.000 claims description 6
- 210000003483 chromatin Anatomy 0.000 claims description 6
- 230000007246 mechanism Effects 0.000 claims description 6
- 230000001105 regulatory effect Effects 0.000 claims description 6
- 230000033228 biological regulation Effects 0.000 claims description 5
- 235000005911 diet Nutrition 0.000 claims description 5
- 230000000378 dietary effect Effects 0.000 claims description 5
- 210000001671 embryonic stem cell Anatomy 0.000 claims description 5
- 230000029279 positive regulation of transcription, DNA-dependent Effects 0.000 claims description 5
- 108091028043 Nucleic acid sequence Proteins 0.000 claims description 4
- 239000003226 mitogen Substances 0.000 claims description 4
- 244000144977 poultry Species 0.000 claims description 4
- 241000283690 Bos taurus Species 0.000 claims description 3
- 101000958866 Homo sapiens Myogenic factor 6 Proteins 0.000 claims description 3
- 101000601661 Homo sapiens Paired box protein Pax-7 Proteins 0.000 claims description 3
- 102100038379 Myogenic factor 6 Human genes 0.000 claims description 3
- 102100037503 Paired box protein Pax-7 Human genes 0.000 claims description 3
- 239000003153 chemical reaction reagent Substances 0.000 claims description 3
- 210000001082 somatic cell Anatomy 0.000 claims description 3
- 230000003213 activating effect Effects 0.000 claims description 2
- 238000012258 culturing Methods 0.000 claims 3
- 101001023043 Homo sapiens Myoblast determination protein 1 Proteins 0.000 claims 1
- 101000589002 Homo sapiens Myogenin Proteins 0.000 claims 1
- 102100035077 Myoblast determination protein 1 Human genes 0.000 claims 1
- AQGNHMOJWBZFQQ-UHFFFAOYSA-N CT 99021 Chemical compound CC1=CNC(C=2C(=NC(NCCNC=3N=CC(=CC=3)C#N)=NC=2)C=2C(=CC(Cl)=CC=2)Cl)=N1 AQGNHMOJWBZFQQ-UHFFFAOYSA-N 0.000 description 39
- 230000011712 cell development Effects 0.000 description 36
- 229960003722 doxycycline Drugs 0.000 description 33
- XQTWDDCIUJNLTR-CVHRZJFOSA-N doxycycline monohydrate Chemical compound O.O=C1C2=C(O)C=CC=C2[C@H](C)[C@@H]2C1=C(O)[C@]1(O)C(=O)C(C(N)=O)=C(O)[C@@H](N(C)C)[C@@H]1[C@H]2O XQTWDDCIUJNLTR-CVHRZJFOSA-N 0.000 description 33
- 230000006698 induction Effects 0.000 description 22
- 235000013372 meat Nutrition 0.000 description 20
- 239000002609 medium Substances 0.000 description 20
- 241000894007 species Species 0.000 description 20
- 238000001262 western blot Methods 0.000 description 19
- 210000000130 stem cell Anatomy 0.000 description 18
- 241001465754 Metazoa Species 0.000 description 17
- 238000004519 manufacturing process Methods 0.000 description 17
- 108090000623 proteins and genes Proteins 0.000 description 16
- 108010056785 Myogenin Proteins 0.000 description 15
- 238000005516 engineering process Methods 0.000 description 14
- 230000004913 activation Effects 0.000 description 13
- 230000005283 ground state Effects 0.000 description 13
- 108010085895 Laminin Proteins 0.000 description 12
- 102000007547 Laminin Human genes 0.000 description 12
- 239000003112 inhibitor Substances 0.000 description 12
- 108010082117 matrigel Proteins 0.000 description 12
- 238000001514 detection method Methods 0.000 description 11
- 102100028914 Catenin beta-1 Human genes 0.000 description 10
- 101000916173 Homo sapiens Catenin beta-1 Proteins 0.000 description 10
- 239000004472 Lysine Substances 0.000 description 10
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 10
- 210000002242 embryoid body Anatomy 0.000 description 10
- 230000008569 process Effects 0.000 description 10
- 102000004169 proteins and genes Human genes 0.000 description 10
- 241001529936 Murinae Species 0.000 description 9
- 108700019146 Transgenes Proteins 0.000 description 9
- 230000008901 benefit Effects 0.000 description 9
- 230000007704 transition Effects 0.000 description 9
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 8
- 239000011575 calcium Substances 0.000 description 8
- 229910052791 calcium Inorganic materials 0.000 description 8
- 239000000306 component Substances 0.000 description 8
- 238000002372 labelling Methods 0.000 description 8
- VOXZDWNPVJITMN-ZBRFXRBCSA-N 17β-estradiol Chemical compound OC1=CC=C2[C@H]3CC[C@](C)([C@H](CC4)O)[C@@H]4[C@@H]3CCC2=C1 VOXZDWNPVJITMN-ZBRFXRBCSA-N 0.000 description 7
- NMUSYJAQQFHJEW-KVTDHHQDSA-N 5-azacytidine Chemical compound O=C1N=C(N)N=CN1[C@H]1[C@H](O)[C@H](O)[C@@H](CO)O1 NMUSYJAQQFHJEW-KVTDHHQDSA-N 0.000 description 7
- 108090000672 Annexin A5 Proteins 0.000 description 7
- 102000004121 Annexin A5 Human genes 0.000 description 7
- 102100036912 Desmin Human genes 0.000 description 7
- 108010044052 Desmin Proteins 0.000 description 7
- 230000001464 adherent effect Effects 0.000 description 7
- 210000005045 desmin Anatomy 0.000 description 7
- 230000004927 fusion Effects 0.000 description 7
- 230000005764 inhibitory process Effects 0.000 description 7
- 210000001778 pluripotent stem cell Anatomy 0.000 description 7
- 150000003384 small molecules Chemical class 0.000 description 7
- 241000272517 Anseriformes Species 0.000 description 6
- 102100029855 Caspase-3 Human genes 0.000 description 6
- 101000793880 Homo sapiens Caspase-3 Proteins 0.000 description 6
- 101001139134 Homo sapiens Krueppel-like factor 4 Proteins 0.000 description 6
- 101001094700 Homo sapiens POU domain, class 5, transcription factor 1 Proteins 0.000 description 6
- 102100020677 Krueppel-like factor 4 Human genes 0.000 description 6
- 102100035423 POU domain, class 5, transcription factor 1 Human genes 0.000 description 6
- 238000013459 approach Methods 0.000 description 6
- RYYVLZVUVIJVGH-UHFFFAOYSA-N caffeine Chemical compound CN1C(=O)N(C)C(=O)C2=C1N=CN2C RYYVLZVUVIJVGH-UHFFFAOYSA-N 0.000 description 6
- 230000008602 contraction Effects 0.000 description 6
- 230000000694 effects Effects 0.000 description 6
- 229960005309 estradiol Drugs 0.000 description 6
- 229930182833 estradiol Natural products 0.000 description 6
- 238000000338 in vitro Methods 0.000 description 6
- 238000002955 isolation Methods 0.000 description 6
- 238000011068 loading method Methods 0.000 description 6
- 210000003716 mesoderm Anatomy 0.000 description 6
- 210000002966 serum Anatomy 0.000 description 6
- 230000000638 stimulation Effects 0.000 description 6
- 239000013598 vector Substances 0.000 description 6
- NMUSYJAQQFHJEW-UHFFFAOYSA-N 5-Azacytidine Natural products O=C1N=C(N)N=CN1C1C(O)C(O)C(CO)O1 NMUSYJAQQFHJEW-UHFFFAOYSA-N 0.000 description 5
- -1 BIO Chemical compound 0.000 description 5
- 229960002756 azacitidine Drugs 0.000 description 5
- 230000000670 limiting effect Effects 0.000 description 5
- 210000004940 nucleus Anatomy 0.000 description 5
- 230000026731 phosphorylation Effects 0.000 description 5
- 238000006366 phosphorylation reaction Methods 0.000 description 5
- 239000000758 substrate Substances 0.000 description 5
- 230000004083 survival effect Effects 0.000 description 5
- 238000004114 suspension culture Methods 0.000 description 5
- 230000001052 transient effect Effects 0.000 description 5
- 108010010803 Gelatin Proteins 0.000 description 4
- 102000005604 Myosin Heavy Chains Human genes 0.000 description 4
- 108010084498 Myosin Heavy Chains Proteins 0.000 description 4
- BELBBZDIHDAJOR-UHFFFAOYSA-N Phenolsulfonephthalein Chemical compound C1=CC(O)=CC=C1C1(C=2C=CC(O)=CC=2)C2=CC=CC=C2S(=O)(=O)O1 BELBBZDIHDAJOR-UHFFFAOYSA-N 0.000 description 4
- 108010029485 Protein Isoforms Proteins 0.000 description 4
- 102000001708 Protein Isoforms Human genes 0.000 description 4
- 102000013814 Wnt Human genes 0.000 description 4
- 108050003627 Wnt Proteins 0.000 description 4
- 101150063416 add gene Proteins 0.000 description 4
- 229930189065 blasticidin Natural products 0.000 description 4
- 238000006243 chemical reaction Methods 0.000 description 4
- 238000011161 development Methods 0.000 description 4
- 230000018109 developmental process Effects 0.000 description 4
- 239000012636 effector Substances 0.000 description 4
- 230000002708 enhancing effect Effects 0.000 description 4
- 238000000684 flow cytometry Methods 0.000 description 4
- 239000012634 fragment Substances 0.000 description 4
- 229920000159 gelatin Polymers 0.000 description 4
- 239000008273 gelatin Substances 0.000 description 4
- 235000019322 gelatine Nutrition 0.000 description 4
- 235000011852 gelatine desserts Nutrition 0.000 description 4
- 238000012239 gene modification Methods 0.000 description 4
- 230000005017 genetic modification Effects 0.000 description 4
- 235000013617 genetically modified food Nutrition 0.000 description 4
- 230000001965 increasing effect Effects 0.000 description 4
- 239000003446 ligand Substances 0.000 description 4
- 150000007523 nucleic acids Chemical group 0.000 description 4
- 229960003531 phenolsulfonphthalein Drugs 0.000 description 4
- 239000000047 product Substances 0.000 description 4
- 230000000717 retained effect Effects 0.000 description 4
- 230000022379 skeletal muscle tissue development Effects 0.000 description 4
- 230000008685 targeting Effects 0.000 description 4
- 230000009495 transient activation Effects 0.000 description 4
- 241000282412 Homo Species 0.000 description 3
- LPHGQDQBBGAPDZ-UHFFFAOYSA-N Isocaffeine Natural products CN1C(=O)N(C)C(=O)C2=C1N(C)C=N2 LPHGQDQBBGAPDZ-UHFFFAOYSA-N 0.000 description 3
- 102100032352 Leukemia inhibitory factor Human genes 0.000 description 3
- 108090000581 Leukemia inhibitory factor Proteins 0.000 description 3
- 102000001068 Neural Cell Adhesion Molecules Human genes 0.000 description 3
- 108010069196 Neural Cell Adhesion Molecules Proteins 0.000 description 3
- OIPILFWXSMYKGL-UHFFFAOYSA-N acetylcholine Chemical compound CC(=O)OCC[N+](C)(C)C OIPILFWXSMYKGL-UHFFFAOYSA-N 0.000 description 3
- 229960004373 acetylcholine Drugs 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 3
- 230000025164 anoikis Effects 0.000 description 3
- 230000001640 apoptogenic effect Effects 0.000 description 3
- 230000015572 biosynthetic process Effects 0.000 description 3
- 229960001948 caffeine Drugs 0.000 description 3
- VJEONQKOZGKCAK-UHFFFAOYSA-N caffeine Natural products CN1C(=O)N(C)C(=O)C2=C1C=CN2C VJEONQKOZGKCAK-UHFFFAOYSA-N 0.000 description 3
- 230000022131 cell cycle Effects 0.000 description 3
- 230000001419 dependent effect Effects 0.000 description 3
- 238000001983 electron spin resonance imaging Methods 0.000 description 3
- 238000009472 formulation Methods 0.000 description 3
- 230000001976 improved effect Effects 0.000 description 3
- 108020001756 ligand binding domains Proteins 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 230000004048 modification Effects 0.000 description 3
- 238000012986 modification Methods 0.000 description 3
- 210000003365 myofibril Anatomy 0.000 description 3
- 102000039446 nucleic acids Human genes 0.000 description 3
- 108020004707 nucleic acids Proteins 0.000 description 3
- 239000013612 plasmid Substances 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 230000011664 signaling Effects 0.000 description 3
- 230000001225 therapeutic effect Effects 0.000 description 3
- FWBHETKCLVMNFS-UHFFFAOYSA-N 4',6-Diamino-2-phenylindol Chemical compound C1=CC(C(=N)N)=CC=C1C1=CC2=CC=C(C(N)=N)C=C2N1 FWBHETKCLVMNFS-UHFFFAOYSA-N 0.000 description 2
- 241000251468 Actinopterygii Species 0.000 description 2
- 241000269350 Anura Species 0.000 description 2
- 241000030939 Bubalus bubalis Species 0.000 description 2
- 241000282832 Camelidae Species 0.000 description 2
- 241000282472 Canis lupus familiaris Species 0.000 description 2
- 241000283707 Capra Species 0.000 description 2
- 108010076667 Caspases Proteins 0.000 description 2
- 102000011727 Caspases Human genes 0.000 description 2
- 241000238366 Cephalopoda Species 0.000 description 2
- 241000282994 Cervidae Species 0.000 description 2
- 241000283153 Cetacea Species 0.000 description 2
- 241000272201 Columbiformes Species 0.000 description 2
- 241000766026 Coregonus nasus Species 0.000 description 2
- 241000270722 Crocodylidae Species 0.000 description 2
- 241000238424 Crustacea Species 0.000 description 2
- 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 description 2
- 241000283086 Equidae Species 0.000 description 2
- 102000010834 Extracellular Matrix Proteins Human genes 0.000 description 2
- 108010037362 Extracellular Matrix Proteins Proteins 0.000 description 2
- 241000282326 Felis catus Species 0.000 description 2
- 241000287828 Gallus gallus Species 0.000 description 2
- 108700039691 Genetic Promoter Regions Proteins 0.000 description 2
- 108010033040 Histones Proteins 0.000 description 2
- 102000006947 Histones Human genes 0.000 description 2
- 101000882584 Homo sapiens Estrogen receptor Proteins 0.000 description 2
- 102100021291 Methyl-CpG-binding domain protein 3 Human genes 0.000 description 2
- 101710111894 Methyl-CpG-binding domain protein 3 Proteins 0.000 description 2
- 241000237852 Mollusca Species 0.000 description 2
- 241000699670 Mus sp. Species 0.000 description 2
- 108700026244 Open Reading Frames Proteins 0.000 description 2
- 241000283973 Oryctolagus cuniculus Species 0.000 description 2
- 241001494479 Pecora Species 0.000 description 2
- 241000286209 Phasianidae Species 0.000 description 2
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 2
- 241000288906 Primates Species 0.000 description 2
- RJKFOVLPORLFTN-LEKSSAKUSA-N Progesterone Chemical compound C1CC2=CC(=O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H](C(=O)C)[C@@]1(C)CC2 RJKFOVLPORLFTN-LEKSSAKUSA-N 0.000 description 2
- 241000700159 Rattus Species 0.000 description 2
- 241000283984 Rodentia Species 0.000 description 2
- 230000018199 S phase Effects 0.000 description 2
- 241000282887 Suidae Species 0.000 description 2
- NKANXQFJJICGDU-QPLCGJKRSA-N Tamoxifen Chemical compound C=1C=CC=CC=1C(/CC)=C(C=1C=CC(OCCN(C)C)=CC=1)/C1=CC=CC=C1 NKANXQFJJICGDU-QPLCGJKRSA-N 0.000 description 2
- 206010043276 Teratoma Diseases 0.000 description 2
- 241000270666 Testudines Species 0.000 description 2
- 230000000692 anti-sense effect Effects 0.000 description 2
- 238000009360 aquaculture Methods 0.000 description 2
- 244000144974 aquaculture Species 0.000 description 2
- 239000007640 basal medium Substances 0.000 description 2
- 238000003352 cell adhesion assay Methods 0.000 description 2
- 238000004113 cell culture Methods 0.000 description 2
- 239000002458 cell surface marker Substances 0.000 description 2
- 238000003776 cleavage reaction Methods 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 210000002744 extracellular matrix Anatomy 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 235000012041 food component Nutrition 0.000 description 2
- 230000009368 gene silencing by RNA Effects 0.000 description 2
- 238000010362 genome editing Methods 0.000 description 2
- 238000001727 in vivo Methods 0.000 description 2
- 238000003780 insertion Methods 0.000 description 2
- 230000037431 insertion Effects 0.000 description 2
- KWGKDLIKAYFUFQ-UHFFFAOYSA-M lithium chloride Chemical compound [Li+].[Cl-] KWGKDLIKAYFUFQ-UHFFFAOYSA-M 0.000 description 2
- 239000006166 lysate Substances 0.000 description 2
- 238000012423 maintenance Methods 0.000 description 2
- 239000003550 marker Substances 0.000 description 2
- 230000035800 maturation Effects 0.000 description 2
- 230000005012 migration Effects 0.000 description 2
- 238000013508 migration Methods 0.000 description 2
- 210000003098 myoblast Anatomy 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 230000037361 pathway Effects 0.000 description 2
- 238000002135 phase contrast microscopy Methods 0.000 description 2
- 102000040430 polynucleotide Human genes 0.000 description 2
- 108091033319 polynucleotide Proteins 0.000 description 2
- 239000002157 polynucleotide Substances 0.000 description 2
- XJMOSONTPMZWPB-UHFFFAOYSA-M propidium iodide Chemical compound [I-].[I-].C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CCC[N+](C)(CC)CC)=C1C1=CC=CC=C1 XJMOSONTPMZWPB-UHFFFAOYSA-M 0.000 description 2
- 238000005215 recombination Methods 0.000 description 2
- 230000002829 reductive effect Effects 0.000 description 2
- 230000008672 reprogramming Effects 0.000 description 2
- 230000007017 scission Effects 0.000 description 2
- 239000012679 serum free medium Substances 0.000 description 2
- 230000002269 spontaneous effect Effects 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- 238000013518 transcription Methods 0.000 description 2
- 230000035897 transcription Effects 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- 230000009466 transformation Effects 0.000 description 2
- 238000004627 transmission electron microscopy Methods 0.000 description 2
- HPTXLHAHLXOAKV-INIZCTEOSA-N (2S)-2-(1,3-dioxo-2-isoindolyl)-3-(1H-indol-3-yl)propanoic acid Chemical compound O=C1C2=CC=CC=C2C(=O)N1[C@H](C(=O)O)CC1=CNC2=CC=CC=C12 HPTXLHAHLXOAKV-INIZCTEOSA-N 0.000 description 1
- PTNZGHXUZDHMIQ-CVHRZJFOSA-N (4s,4ar,5s,5ar,6r,12ar)-4-(dimethylamino)-1,5,10,11,12a-pentahydroxy-6-methyl-3,12-dioxo-4a,5,5a,6-tetrahydro-4h-tetracene-2-carboxamide;hydrochloride Chemical compound Cl.C1=CC=C2[C@H](C)[C@@H]([C@H](O)[C@@H]3[C@](C(O)=C(C(N)=O)C(=O)[C@H]3N(C)C)(O)C3=O)C3=C(O)C2=C1O PTNZGHXUZDHMIQ-CVHRZJFOSA-N 0.000 description 1
- SYSZENVIJHPFNL-UHFFFAOYSA-N (alpha-D-mannosyl)7-beta-D-mannosyl-diacetylchitobiosyl-L-asparagine, isoform B (protein) Chemical compound COC1=CC=C(I)C=C1 SYSZENVIJHPFNL-UHFFFAOYSA-N 0.000 description 1
- FPIPGXGPPPQFEQ-UHFFFAOYSA-N 13-cis retinol Natural products OCC=C(C)C=CC=C(C)C=CC1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-UHFFFAOYSA-N 0.000 description 1
- DXYYSGDWQCSKKO-UHFFFAOYSA-N 2-methylbenzothiazole Chemical compound C1=CC=C2SC(C)=NC2=C1 DXYYSGDWQCSKKO-UHFFFAOYSA-N 0.000 description 1
- MDYDGUOQFUQOGE-UHFFFAOYSA-N 2-methylpropanethioic acid S-[7-oxo-7-[(4-phenyl-2-thiazolyl)amino]heptyl] ester Chemical compound S1C(NC(=O)CCCCCCSC(=O)C(C)C)=NC(C=2C=CC=CC=2)=C1 MDYDGUOQFUQOGE-UHFFFAOYSA-N 0.000 description 1
- JCSGFHVFHSKIJH-UHFFFAOYSA-N 3-(2,4-dichlorophenyl)-4-(1-methyl-3-indolyl)pyrrole-2,5-dione Chemical compound C12=CC=CC=C2N(C)C=C1C(C(NC1=O)=O)=C1C1=CC=C(Cl)C=C1Cl JCSGFHVFHSKIJH-UHFFFAOYSA-N 0.000 description 1
- MAUCONCHVWBMHK-UHFFFAOYSA-N 3-[(dimethylamino)methyl]-N-[2-[4-[(hydroxyamino)-oxomethyl]phenoxy]ethyl]-2-benzofurancarboxamide Chemical compound O1C2=CC=CC=C2C(CN(C)C)=C1C(=O)NCCOC1=CC=C(C(=O)NO)C=C1 MAUCONCHVWBMHK-UHFFFAOYSA-N 0.000 description 1
- ZKJAZFUFPPSFCO-UHFFFAOYSA-N 3-[2-(4-fluorophenyl)ethylamino]-1-methyl-4-(2-methyl-1H-indol-3-yl)pyrrole-2,5-dione Chemical compound CC=1NC2=CC=CC=C2C=1C=1C(=O)N(C)C(=O)C=1NCCC1=CC=C(F)C=C1 ZKJAZFUFPPSFCO-UHFFFAOYSA-N 0.000 description 1
- VPVLEBIVXZSOMQ-UHFFFAOYSA-N 3-[[6-(3-aminophenyl)-7H-pyrrolo[2,3-d]pyrimidin-4-yl]oxy]phenol Chemical compound NC1=CC=CC(C=2NC3=NC=NC(OC=4C=C(O)C=CC=4)=C3C=2)=C1 VPVLEBIVXZSOMQ-UHFFFAOYSA-N 0.000 description 1
- JHSXDAWGLCZYSM-UHFFFAOYSA-N 4-(4-chloro-2-methylphenoxy)-N-hydroxybutanamide Chemical compound CC1=CC(Cl)=CC=C1OCCCC(=O)NO JHSXDAWGLCZYSM-UHFFFAOYSA-N 0.000 description 1
- DVNYTAVYBRSTGK-UHFFFAOYSA-N 5-aminoimidazole-4-carboxamide Chemical compound NC(=O)C=1N=CNC=1N DVNYTAVYBRSTGK-UHFFFAOYSA-N 0.000 description 1
- XAUDJQYHKZQPEU-KVQBGUIXSA-N 5-aza-2'-deoxycytidine Chemical compound O=C1N=C(N)N=CN1[C@@H]1O[C@H](CO)[C@@H](O)C1 XAUDJQYHKZQPEU-KVQBGUIXSA-N 0.000 description 1
- RZWRYPGAUIOOMK-UHFFFAOYSA-N 5-nitroso-8-quinolinol Chemical compound C1=CN=C2C(O)=CC=C(N=O)C2=C1 RZWRYPGAUIOOMK-UHFFFAOYSA-N 0.000 description 1
- JTDYUFSDZATMKU-UHFFFAOYSA-N 6-(1,3-dioxo-2-benzo[de]isoquinolinyl)-N-hydroxyhexanamide Chemical compound C1=CC(C(N(CCCCCC(=O)NO)C2=O)=O)=C3C2=CC=CC3=C1 JTDYUFSDZATMKU-UHFFFAOYSA-N 0.000 description 1
- VUWMSJQZENJPHO-UHFFFAOYSA-N 7-oxo-7-(n-phenylanilino)heptanoic acid Chemical compound C=1C=CC=CC=1N(C(=O)CCCCCC(=O)O)C1=CC=CC=C1 VUWMSJQZENJPHO-UHFFFAOYSA-N 0.000 description 1
- HJCMDXDYPOUFDY-WHFBIAKZSA-N Ala-Gln Chemical compound C[C@H](N)C(=O)N[C@H](C(O)=O)CCC(N)=O HJCMDXDYPOUFDY-WHFBIAKZSA-N 0.000 description 1
- 108700028369 Alleles Proteins 0.000 description 1
- 241001550224 Apha Species 0.000 description 1
- 239000012583 B-27 Supplement Substances 0.000 description 1
- RFLHBLWLFUFFDZ-UHFFFAOYSA-N BML-210 Chemical compound NC1=CC=CC=C1NC(=O)CCCCCCC(=O)NC1=CC=CC=C1 RFLHBLWLFUFFDZ-UHFFFAOYSA-N 0.000 description 1
- 102000015735 Beta-catenin Human genes 0.000 description 1
- 108060000903 Beta-catenin Proteins 0.000 description 1
- 239000002028 Biomass Substances 0.000 description 1
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 1
- MDZCSIDIPDZWKL-UHFFFAOYSA-N CHIR-98014 Chemical compound C1=C([N+]([O-])=O)C(N)=NC(NCCNC=2N=C(C(=CN=2)N2C=NC=C2)C=2C(=CC(Cl)=CC=2)Cl)=C1 MDZCSIDIPDZWKL-UHFFFAOYSA-N 0.000 description 1
- 108091033409 CRISPR Proteins 0.000 description 1
- 238000010354 CRISPR gene editing Methods 0.000 description 1
- 101100328086 Caenorhabditis elegans cla-1 gene Proteins 0.000 description 1
- 108090000397 Caspase 3 Proteins 0.000 description 1
- 102000003952 Caspase 3 Human genes 0.000 description 1
- 102000016362 Catenins Human genes 0.000 description 1
- 108010067316 Catenins Proteins 0.000 description 1
- 108010009685 Cholinergic Receptors Proteins 0.000 description 1
- 108091026890 Coding region Proteins 0.000 description 1
- 108020004705 Codon Proteins 0.000 description 1
- KDXKERNSBIXSRK-RXMQYKEDSA-N D-lysine group Chemical group N[C@H](CCCCN)C(=O)O KDXKERNSBIXSRK-RXMQYKEDSA-N 0.000 description 1
- 108020004414 DNA Proteins 0.000 description 1
- 230000007067 DNA methylation Effects 0.000 description 1
- 102000016680 Dioxygenases Human genes 0.000 description 1
- 108010028143 Dioxygenases Proteins 0.000 description 1
- 102000008157 Histone Demethylases Human genes 0.000 description 1
- 108010074870 Histone Demethylases Proteins 0.000 description 1
- 102000011787 Histone Methyltransferases Human genes 0.000 description 1
- 108010036115 Histone Methyltransferases Proteins 0.000 description 1
- 102000003893 Histone acetyltransferases Human genes 0.000 description 1
- 108090000246 Histone acetyltransferases Proteins 0.000 description 1
- 102000003964 Histone deacetylase Human genes 0.000 description 1
- 108090000353 Histone deacetylase Proteins 0.000 description 1
- 101000942967 Homo sapiens Leukemia inhibitory factor Proteins 0.000 description 1
- 101000582994 Homo sapiens Myelin regulatory factor Proteins 0.000 description 1
- 101150088952 IGF1 gene Proteins 0.000 description 1
- 102000016397 Methyltransferase Human genes 0.000 description 1
- 108060004795 Methyltransferase Proteins 0.000 description 1
- 102100030372 Myelin regulatory factor Human genes 0.000 description 1
- 101710099061 Myogenic factor 5 Proteins 0.000 description 1
- HRNLUBSXIHFDHP-UHFFFAOYSA-N N-(2-aminophenyl)-4-[[[4-(3-pyridinyl)-2-pyrimidinyl]amino]methyl]benzamide Chemical compound NC1=CC=CC=C1NC(=O)C(C=C1)=CC=C1CNC1=NC=CC(C=2C=NC=CC=2)=N1 HRNLUBSXIHFDHP-UHFFFAOYSA-N 0.000 description 1
- 239000012580 N-2 Supplement Substances 0.000 description 1
- YALNUENQHAQXEA-UHFFFAOYSA-N N-[4-[(hydroxyamino)-oxomethyl]phenyl]carbamic acid [6-(diethylaminomethyl)-2-naphthalenyl]methyl ester Chemical compound C1=CC2=CC(CN(CC)CC)=CC=C2C=C1COC(=O)NC1=CC=C(C(=O)NO)C=C1 YALNUENQHAQXEA-UHFFFAOYSA-N 0.000 description 1
- WWGBHDIHIVGYLZ-UHFFFAOYSA-N N-[4-[3-[[[7-(hydroxyamino)-7-oxoheptyl]amino]-oxomethyl]-5-isoxazolyl]phenyl]carbamic acid tert-butyl ester Chemical compound C1=CC(NC(=O)OC(C)(C)C)=CC=C1C1=CC(C(=O)NCCCCCCC(=O)NO)=NO1 WWGBHDIHIVGYLZ-UHFFFAOYSA-N 0.000 description 1
- KPNNXHVGOKRBEF-UHFFFAOYSA-N N-hydroxy-7-(2-naphthalenylthio)heptanamide Chemical compound C1=CC=CC2=CC(SCCCCCCC(=O)NO)=CC=C21 KPNNXHVGOKRBEF-UHFFFAOYSA-N 0.000 description 1
- 240000007019 Oxalis corniculata Species 0.000 description 1
- 208000020584 Polyploidy Diseases 0.000 description 1
- 241001112090 Pseudovirus Species 0.000 description 1
- 238000012228 RNA interference-mediated gene silencing Methods 0.000 description 1
- 108091030071 RNAI Proteins 0.000 description 1
- 102100024837 SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily D member 3 Human genes 0.000 description 1
- 101710188227 SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily D member 3 Proteins 0.000 description 1
- MTCFGRXMJLQNBG-UHFFFAOYSA-N Serine Natural products OCC(N)C(O)=O MTCFGRXMJLQNBG-UHFFFAOYSA-N 0.000 description 1
- 108091027967 Small hairpin RNA Proteins 0.000 description 1
- 238000010459 TALEN Methods 0.000 description 1
- AYFVYJQAPQTCCC-UHFFFAOYSA-N Threonine Natural products CC(O)C(N)C(O)=O AYFVYJQAPQTCCC-UHFFFAOYSA-N 0.000 description 1
- 239000004473 Threonine Substances 0.000 description 1
- AUYYCJSJGJYCDS-LBPRGKRZSA-N Thyrolar Chemical class IC1=CC(C[C@H](N)C(O)=O)=CC(I)=C1OC1=CC=C(O)C(I)=C1 AUYYCJSJGJYCDS-LBPRGKRZSA-N 0.000 description 1
- 108010043645 Transcription Activator-Like Effector Nucleases Proteins 0.000 description 1
- 230000010632 Transcription Factor Activity Effects 0.000 description 1
- RTKIYFITIVXBLE-UHFFFAOYSA-N Trichostatin A Natural products ONC(=O)C=CC(C)=CC(C)C(=O)C1=CC=C(N(C)C)C=C1 RTKIYFITIVXBLE-UHFFFAOYSA-N 0.000 description 1
- 102000004243 Tubulin Human genes 0.000 description 1
- 108090000704 Tubulin Proteins 0.000 description 1
- FPIPGXGPPPQFEQ-BOOMUCAASA-N Vitamin A Natural products OC/C=C(/C)\C=C\C=C(\C)/C=C/C1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-BOOMUCAASA-N 0.000 description 1
- 108010017070 Zinc Finger Nucleases Proteins 0.000 description 1
- FKAWLXNLHHIHLA-YCBIHMBMSA-N [(2r,3r,5r,7r,8s,9s)-2-[(1s,3s,4s,5r,6r,7e,9e,11e,13z)-14-cyano-3,5-dihydroxy-1-methoxy-4,6,8,9,13-pentamethyltetradeca-7,9,11,13-tetraenyl]-9-[(e)-3-[2-[(2s)-4-[[(2s,3s,4s)-4-(dimethylamino)-2,3-dihydroxy-5-methoxypentanoyl]amino]butan-2-yl]-1,3-oxazol-4 Chemical compound O1C([C@@H](C)CCNC(=O)[C@@H](O)[C@@H](O)[C@H](COC)N(C)C)=NC(\C=C\C[C@H]2[C@H]([C@H](O)C[C@]3(O2)C([C@@H](OP(O)(O)=O)[C@@H]([C@H](C[C@H](O)[C@H](C)[C@H](O)[C@H](C)\C=C(/C)\C(\C)=C\C=C\C(\C)=C/C#N)OC)O3)(C)C)C)=C1 FKAWLXNLHHIHLA-YCBIHMBMSA-N 0.000 description 1
- 229950008805 abexinostat Drugs 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 102000034337 acetylcholine receptors Human genes 0.000 description 1
- QOMNQGZXFYNBNG-UHFFFAOYSA-N acetyloxymethyl 2-[2-[2-[5-[3-(acetyloxymethoxy)-2,7-difluoro-6-oxoxanthen-9-yl]-2-[bis[2-(acetyloxymethoxy)-2-oxoethyl]amino]phenoxy]ethoxy]-n-[2-(acetyloxymethoxy)-2-oxoethyl]-4-methylanilino]acetate Chemical compound CC(=O)OCOC(=O)CN(CC(=O)OCOC(C)=O)C1=CC=C(C)C=C1OCCOC1=CC(C2=C3C=C(F)C(=O)C=C3OC3=CC(OCOC(C)=O)=C(F)C=C32)=CC=C1N(CC(=O)OCOC(C)=O)CC(=O)OCOC(C)=O QOMNQGZXFYNBNG-UHFFFAOYSA-N 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- 238000004115 adherent culture Methods 0.000 description 1
- 230000001270 agonistic effect Effects 0.000 description 1
- FPIPGXGPPPQFEQ-OVSJKPMPSA-N all-trans-retinol Chemical compound OC\C=C(/C)\C=C\C=C(/C)\C=C\C1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-OVSJKPMPSA-N 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 150000001413 amino acids Chemical group 0.000 description 1
- 239000003098 androgen Substances 0.000 description 1
- 239000010868 animal carcass Substances 0.000 description 1
- 230000003042 antagnostic effect Effects 0.000 description 1
- 239000005557 antagonist Substances 0.000 description 1
- 230000006907 apoptotic process Effects 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- NCNRHFGMJRPRSK-MDZDMXLPSA-N belinostat Chemical compound ONC(=O)\C=C\C1=CC=CC(S(=O)(=O)NC=2C=CC=CC=2)=C1 NCNRHFGMJRPRSK-MDZDMXLPSA-N 0.000 description 1
- 229960003094 belinostat Drugs 0.000 description 1
- 238000001574 biopsy Methods 0.000 description 1
- 229940098773 bovine serum albumin Drugs 0.000 description 1
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 239000013592 cell lysate Substances 0.000 description 1
- 230000004663 cell proliferation Effects 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- HQSSEGBEYORUBY-WPWMEQJKSA-N chembl597845 Chemical compound C=1C=CC(\N=C\C=2C3=CC=CC=C3C=CC=2O)=CC=1NC(=O)C(C)C1=CC=CC=C1 HQSSEGBEYORUBY-WPWMEQJKSA-N 0.000 description 1
- 238000010367 cloning Methods 0.000 description 1
- 238000010835 comparative analysis Methods 0.000 description 1
- 238000004624 confocal microscopy Methods 0.000 description 1
- 230000034994 death Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 238000012217 deletion Methods 0.000 description 1
- 230000037430 deletion Effects 0.000 description 1
- 238000010494 dissociation reaction Methods 0.000 description 1
- 230000005593 dissociations Effects 0.000 description 1
- 238000007877 drug screening Methods 0.000 description 1
- INVTYAOGFAGBOE-UHFFFAOYSA-N entinostat Chemical compound NC1=CC=CC=C1NC(=O)C(C=C1)=CC=C1CNC(=O)OCC1=CC=CN=C1 INVTYAOGFAGBOE-UHFFFAOYSA-N 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 239000003797 essential amino acid Substances 0.000 description 1
- 235000020776 essential amino acid Nutrition 0.000 description 1
- 229940011871 estrogen Drugs 0.000 description 1
- 239000000262 estrogen Substances 0.000 description 1
- 102000015694 estrogen receptors Human genes 0.000 description 1
- 108010038795 estrogen receptors Proteins 0.000 description 1
- 230000001747 exhibiting effect Effects 0.000 description 1
- 239000013604 expression vector Substances 0.000 description 1
- 108020001507 fusion proteins Proteins 0.000 description 1
- 102000037865 fusion proteins Human genes 0.000 description 1
- 230000030279 gene silencing Effects 0.000 description 1
- 210000001654 germ layer Anatomy 0.000 description 1
- 239000003862 glucocorticoid Substances 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 102000046645 human LIF Human genes 0.000 description 1
- 229950011479 hyclate Drugs 0.000 description 1
- 238000003384 imaging method Methods 0.000 description 1
- 238000002513 implantation Methods 0.000 description 1
- 238000012606 in vitro cell culture Methods 0.000 description 1
- 238000010874 in vitro model Methods 0.000 description 1
- 238000011065 in-situ storage Methods 0.000 description 1
- 238000010348 incorporation Methods 0.000 description 1
- 230000002452 interceptive effect Effects 0.000 description 1
- 210000001161 mammalian embryo Anatomy 0.000 description 1
- 230000001404 mediated effect Effects 0.000 description 1
- 108091070501 miRNA Proteins 0.000 description 1
- 239000002679 microRNA Substances 0.000 description 1
- 230000000877 morphologic effect Effects 0.000 description 1
- 210000003205 muscle Anatomy 0.000 description 1
- 230000035772 mutation Effects 0.000 description 1
- YAEMHJKFIIIULI-UHFFFAOYSA-N n-(4-methoxybenzyl)-n'-(5-nitro-1,3-thiazol-2-yl)urea Chemical compound C1=CC(OC)=CC=C1CNC(=O)NC1=NC=C([N+]([O-])=O)S1 YAEMHJKFIIIULI-UHFFFAOYSA-N 0.000 description 1
- OYKBQNOPCSXWBL-SNAWJCMRSA-N n-hydroxy-3-[(e)-3-(hydroxyamino)-3-oxoprop-1-enyl]benzamide Chemical compound ONC(=O)\C=C\C1=CC=CC(C(=O)NO)=C1 OYKBQNOPCSXWBL-SNAWJCMRSA-N 0.000 description 1
- 239000002773 nucleotide Substances 0.000 description 1
- 125000003729 nucleotide group Chemical group 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 230000002018 overexpression Effects 0.000 description 1
- 238000004806 packaging method and process Methods 0.000 description 1
- 229960005184 panobinostat Drugs 0.000 description 1
- FWZRWHZDXBDTFK-ZHACJKMWSA-N panobinostat Chemical compound CC1=NC2=CC=C[CH]C2=C1CCNCC1=CC=C(\C=C\C(=O)NO)C=C1 FWZRWHZDXBDTFK-ZHACJKMWSA-N 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 239000013641 positive control Substances 0.000 description 1
- 230000004481 post-translational protein modification Effects 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 239000002243 precursor Substances 0.000 description 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 239000000186 progesterone Substances 0.000 description 1
- 229960003387 progesterone Drugs 0.000 description 1
- 230000002062 proliferating effect Effects 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 102000005962 receptors Human genes 0.000 description 1
- 108020003175 receptors Proteins 0.000 description 1
- 230000006798 recombination Effects 0.000 description 1
- 230000008844 regulatory mechanism Effects 0.000 description 1
- 238000007634 remodeling Methods 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 108091008146 restriction endonucleases Proteins 0.000 description 1
- 210000002235 sarcomere Anatomy 0.000 description 1
- 239000004017 serum-free culture medium Substances 0.000 description 1
- 230000007781 signaling event Effects 0.000 description 1
- 239000004055 small Interfering RNA Substances 0.000 description 1
- 210000001057 smooth muscle myoblast Anatomy 0.000 description 1
- MFBOGIVSZKQAPD-UHFFFAOYSA-M sodium butyrate Chemical compound [Na+].CCCC([O-])=O MFBOGIVSZKQAPD-UHFFFAOYSA-M 0.000 description 1
- 230000000392 somatic effect Effects 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
- 230000024642 stem cell division Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 230000009469 supplementation Effects 0.000 description 1
- 230000008093 supporting effect Effects 0.000 description 1
- VAZAPHZUAVEOMC-UHFFFAOYSA-N tacedinaline Chemical compound C1=CC(NC(=O)C)=CC=C1C(=O)NC1=CC=CC=C1N VAZAPHZUAVEOMC-UHFFFAOYSA-N 0.000 description 1
- 229960001603 tamoxifen Drugs 0.000 description 1
- 239000005495 thyroid hormone Substances 0.000 description 1
- 229940036555 thyroid hormone Drugs 0.000 description 1
- PMJIHLSCWIDGMD-UHFFFAOYSA-N tideglusib Chemical compound O=C1SN(C=2C3=CC=CC=C3C=CC=2)C(=O)N1CC1=CC=CC=C1 PMJIHLSCWIDGMD-UHFFFAOYSA-N 0.000 description 1
- 229950005284 tideglusib Drugs 0.000 description 1
- 230000026683 transduction Effects 0.000 description 1
- 238000010361 transduction Methods 0.000 description 1
- 238000001890 transfection Methods 0.000 description 1
- 239000012096 transfection reagent Substances 0.000 description 1
- 238000003146 transient transfection Methods 0.000 description 1
- RTKIYFITIVXBLE-QEQCGCAPSA-N trichostatin A Chemical compound ONC(=O)/C=C/C(/C)=C/[C@@H](C)C(=O)C1=CC=C(N(C)C)C=C1 RTKIYFITIVXBLE-QEQCGCAPSA-N 0.000 description 1
- 230000001960 triggered effect Effects 0.000 description 1
- 210000002993 trophoblast Anatomy 0.000 description 1
- GOVYBPLHWIEHEJ-UHFFFAOYSA-N tubastatin A Chemical compound C1N(C)CCC2=C1C1=CC=CC=C1N2CC1=CC=C(C(=O)NO)C=C1 GOVYBPLHWIEHEJ-UHFFFAOYSA-N 0.000 description 1
- 235000019155 vitamin A Nutrition 0.000 description 1
- 239000011719 vitamin A Substances 0.000 description 1
- 229940045997 vitamin a Drugs 0.000 description 1
- WAEXFXRVDQXREF-UHFFFAOYSA-N vorinostat Chemical compound ONC(=O)CCCCCCC(=O)NC1=CC=CC=C1 WAEXFXRVDQXREF-UHFFFAOYSA-N 0.000 description 1
- 229960000237 vorinostat Drugs 0.000 description 1
- 238000002689 xenotransplantation Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0658—Skeletal muscle cells, e.g. myocytes, myotubes, myoblasts
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L13/00—Meat products; Meat meal; Preparation or treatment thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/28—Bone marrow; Haematopoietic stem cells; Mesenchymal stem cells of any origin, e.g. adipose-derived stem cells
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/48—Reproductive organs
- A61K35/54—Ovaries; Ova; Ovules; Embryos; Foetal cells; Germ cells
- A61K35/545—Embryonic stem cells; Pluripotent stem cells; Induced pluripotent stem cells; Uncharacterised stem cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/10—Cells modified by introduction of foreign genetic material
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/30—Hormones
- C12N2501/38—Hormones with nuclear receptors
- C12N2501/39—Steroid hormones
- C12N2501/392—Sexual steroids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/40—Regulators of development
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/40—Regulators of development
- C12N2501/415—Wnt; Frizzeled
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/60—Transcription factors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/60—Transcription factors
- C12N2501/602—Sox-2
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/60—Transcription factors
- C12N2501/603—Oct-3/4
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/60—Transcription factors
- C12N2501/604—Klf-4
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/70—Enzymes
- C12N2501/72—Transferases [EC 2.]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/70—Enzymes
- C12N2501/72—Transferases [EC 2.]
- C12N2501/727—Kinases (EC 2.7.)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/999—Small molecules not provided for elsewhere
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
- C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
- C12N2506/45—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from artificially induced pluripotent stem cells
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- General Health & Medical Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Rheumatology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Immunology (AREA)
- Pharmacology & Pharmacy (AREA)
- Veterinary Medicine (AREA)
- Medicinal Chemistry (AREA)
- Reproductive Health (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Virology (AREA)
- Nutrition Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Gynecology & Obstetrics (AREA)
- Hematology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Fodder In General (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201361962068P | 2013-10-30 | 2013-10-30 | |
| PCT/US2014/063250 WO2015066377A1 (en) | 2013-10-30 | 2014-10-30 | Method for scalable skeletal muscle lineage specification and cultivation |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| IL296889A true IL296889A (en) | 2022-12-01 |
Family
ID=53005145
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| IL296889A IL296889A (en) | 2013-10-30 | 2014-10-30 | A method for breeding and genealogical description of quantitative skeletal muscle |
| IL245292A IL245292B2 (en) | 2013-10-30 | 2016-04-24 | Method for scalable skeletal muscle lineage specification and cultivation |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| IL245292A IL245292B2 (en) | 2013-10-30 | 2016-04-24 | Method for scalable skeletal muscle lineage specification and cultivation |
Country Status (10)
| Country | Link |
|---|---|
| US (3) | US20160251625A1 (enExample) |
| EP (1) | EP3071040B2 (enExample) |
| JP (2) | JP6728049B2 (enExample) |
| CN (2) | CN111154726A (enExample) |
| AU (2) | AU2014342180B2 (enExample) |
| BR (1) | BR112016009803B1 (enExample) |
| CA (1) | CA2928726A1 (enExample) |
| ES (1) | ES2782380T5 (enExample) |
| IL (2) | IL296889A (enExample) |
| WO (1) | WO2015066377A1 (enExample) |
Families Citing this family (39)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| BR112016009803B1 (pt) | 2013-10-30 | 2022-02-08 | The Curators Of The University Of Missouri | Método para especificação e cultivo de linhagem muscular esquelética escalonável |
| US11318167B2 (en) | 2015-12-11 | 2022-05-03 | The Johns Hopkins University | Isolation of fusion-competent myoblasts and therapeutic applications thereof related to muscular dystrophy |
| CA3011484A1 (en) * | 2016-01-14 | 2017-07-20 | Memphis Meats, Inc. | Methods for extending the replicative capacity of somatic cells during an ex vivo cultivation process |
| CN109714962A (zh) | 2016-07-11 | 2019-05-03 | 耶路撒冷希伯来大学的益生研究开发有限公司 | 用于在体外培养细胞的系统和方法 |
| ES2985769T3 (es) | 2017-05-06 | 2024-11-07 | Upside Foods Inc | Composiciones y métodos para aumentar la densidad de cultivo de una biomasa celular dentro de una infraestructura de cultivo |
| US20200140821A1 (en) * | 2017-06-07 | 2020-05-07 | Wild Type, Inc. | Ex vivo meat production |
| WO2019014652A1 (en) | 2017-07-13 | 2019-01-17 | Memphis Meats, Inc. | COMPOSITIONS AND METHODS FOR INCREASING THE EFFICACY OF CELL CULTURES USED IN FOOD PRODUCTION |
| BR112020000952A2 (pt) | 2017-07-15 | 2020-07-14 | Aleph Farms | composições de carne cultivada |
| GB2573327B (en) * | 2018-05-03 | 2021-05-12 | Mosa Meat Bv | Apparatus and process for production of tissue from cells |
| US12478571B2 (en) | 2018-12-17 | 2025-11-25 | Pontis Biologics, Inc. | Enzyme based compositions and methods for removing dental calculi |
| EP3969571A4 (en) | 2019-05-14 | 2023-05-24 | Aleph Farms Ltd. | AGGREGATES OF PLURIPOTENT CELLS AND THEIR USE |
| CA3200542A1 (en) | 2019-05-28 | 2020-12-03 | Upside Foods, Inc. | Apparatuses and methods for preparing a comestible meat product |
| IL292318B2 (en) | 2019-11-20 | 2023-04-01 | Upside Foods Inc | Devices and methods for preparing a meat product |
| CN115443330A (zh) * | 2019-12-02 | 2022-12-06 | 奥方特蛋白质科技有限公司 | 使用体外细胞培养生产肉类的方法 |
| US20210235733A1 (en) | 2020-02-04 | 2021-08-05 | Memphis Meats, Inc. | Characteristics of meat products |
| WO2021248141A1 (en) | 2020-06-05 | 2021-12-09 | Upside Foods, Inc. | Nutrient media for the production of slaughter-free meat |
| GB202008821D0 (en) * | 2020-06-10 | 2020-07-22 | Highersteaks Ltd | Systems and methods for cell conversion |
| US12252681B2 (en) | 2020-08-18 | 2025-03-18 | Upside Foods, Inc. | Systems, devices, and methods for sterilizing bioreactors and culture media |
| US20220195368A1 (en) * | 2020-12-21 | 2022-06-23 | Upside Foods, Inc. | Fluid conduit with radial expansion of fluid flow |
| US11912967B2 (en) | 2020-12-22 | 2024-02-27 | Upside Foods, Inc. | Systems and methods for cultivating tissue on porous substrates |
| WO2022156939A1 (en) * | 2021-01-22 | 2022-07-28 | HigherSteaks Limited | Products for cell conversion |
| EP4304383A4 (en) | 2021-03-10 | 2025-02-26 | Terasaki Institute For Biomedical Innovation | GROWTH FACTORS FOR LABORATORY-GROWN MEAT AND OTHER APPLICATIONS |
| AU2022254053A1 (en) | 2021-04-07 | 2023-10-12 | Upside Foods, Inc. | Generation of cell-based products for consumption that comprise proteins derived from exotic, endangered, and extinct species |
| CN117795058A (zh) | 2021-04-28 | 2024-03-29 | 阿普赛德食品公司 | 生产用于人类消费的基于细胞的产品 |
| BR112023022889A2 (pt) | 2021-05-06 | 2024-01-23 | Yeda Res & Dev | Método para induzir fibras musculares hipertróficas para produção de carne industrial |
| JP2024520498A (ja) * | 2021-05-28 | 2024-05-24 | エービーエス グローバル インコーポレイテッド | 家畜におけるインビトロ育種システム及び方法 |
| JP2024523133A (ja) | 2021-06-11 | 2024-06-28 | アップサイド フーズ， インコーポレイテッド | 乾燥細胞粉末を含む細胞ベースの肉製品、及びそのような製品の製造方法 |
| CA3221762A1 (en) | 2021-06-16 | 2022-12-22 | Upside Foods, Inc. | Plant fat-based scaffolds for the growth of cell-based meats and methods of making such products |
| EP4367219A1 (en) | 2021-07-09 | 2024-05-15 | Suprême | Foodstuffs comprising cells differentiated from engineered oligopotent stem cells |
| CA3231125A1 (en) | 2021-10-19 | 2023-04-27 | Joshua MARCH | Plant base/animal cell hybrid meat substitute |
| IL313347A (en) * | 2021-12-23 | 2024-08-01 | Upside Foods Inc | Myogenic tissue augmentation: late transition myogenicity |
| EP4292440A1 (en) | 2022-06-14 | 2023-12-20 | Upside Foods, Inc. | Suspension based chicken product formulation |
| EP4293109A1 (en) | 2022-06-14 | 2023-12-20 | Upside Foods, Inc. | Non-skeletal muscle-derived cells as a source of suspension capable myogenic cells for cultured foods |
| US11981884B2 (en) | 2022-10-17 | 2024-05-14 | Upside Foods, Inc. | Pipe-based bioreactors for producing comestible meat products and methods of using the same |
| WO2025011578A1 (zh) * | 2023-07-10 | 2025-01-16 | 中国农业大学 | 一种猪多能干细胞的肌源性分化方法及其用于细胞培养肉制备 |
| EP4497814A1 (de) | 2023-07-28 | 2025-01-29 | Green Elephant Biotech GmbH | Vorrichtung zur vermehrung von differenzierten und undifferenzierten zellen und verwendung der vorrichtung für die herstellung von kultivierten lebensmitteln |
| WO2025049891A1 (en) | 2023-09-01 | 2025-03-06 | Upside Foods, Inc. | Transposon system and methods of using the same |
| US20250120418A1 (en) * | 2023-10-12 | 2025-04-17 | Btl Healthcare Technologies A.S. | Pet food, methods and devices for producing pet food |
| WO2025141211A1 (en) * | 2023-12-29 | 2025-07-03 | Meatable B.V. | Cells for forward programming |
Family Cites Families (38)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5510254A (en) | 1986-04-18 | 1996-04-23 | Advanced Tissue Sciences, Inc. | Three dimensional cell and tissue culture system |
| AU642184B2 (en) * | 1989-12-21 | 1993-10-14 | University Of Georgia Research Foundation, Inc., The | Bovine myogenic factor gene |
| US5298422A (en) * | 1991-11-06 | 1994-03-29 | Baylor College Of Medicine | Myogenic vector systems |
| WO1999031222A1 (en) | 1997-12-18 | 1999-06-24 | Willem Frederik Van Eelen | Industrial scale production of meat from in vitro cell cultures |
| WO1999043786A2 (en) | 1998-02-27 | 1999-09-02 | Purdue Research Foundation | Submucosa gel compositions |
| US6537782B1 (en) | 1998-06-01 | 2003-03-25 | Chugai Seiyaku Kabushiki Kaisha | Media for culturing animal cells and process for producing protein by using the same |
| US6835390B1 (en) | 2000-11-17 | 2004-12-28 | Jon Vein | Method for producing tissue engineered meat for consumption |
| EP1639097B1 (en) * | 2003-06-25 | 2013-08-07 | Ottawa Health Research Institute | Methods and compositions for modulating stem cell growth and differentiation |
| DE102004017484B4 (de) | 2004-04-08 | 2006-07-06 | Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. | Verfahren zur Herstellung einer biologischen Materialzusammensetzung tierischen Ursprungs |
| US20060121006A1 (en) | 2004-09-10 | 2006-06-08 | Chancellor Michael B | Production of nutritional and therapeutic products from cultured animal cells |
| WO2006041429A2 (en) | 2004-09-17 | 2006-04-20 | Jon Vein | Tissue engineered meat for consumption and a method for producing tissue engineered meat for consumption |
| JP2009514824A (ja) * | 2005-11-02 | 2009-04-09 | ネステク ソシエテ アノニム | 雄の哺乳動物における体脂肪の蓄積を低減するためのイソフラボン組成物及びその使用方法 |
| KR20080108487A (ko) * | 2006-02-28 | 2008-12-15 | 트르스티스 오브 보스톤 유니버시티 | 대사 조절인자 및 그의 용도 |
| DE102007029699B4 (de) | 2007-06-27 | 2010-12-02 | Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. | Isolierte proliferierende Zellen mit Stammzelleigenschaften aus adultem Gewebe von wechselwarmen Wirbeltieren, stabile Zellkulturen davon und Verfahren zu deren Herstellung |
| CN101624570B (zh) | 2008-07-07 | 2011-04-13 | 刘志军 | 中药培养养殖肉灵芝方法及养殖肉灵芝保健品的制备方法 |
| WO2010068897A2 (en) | 2008-12-12 | 2010-06-17 | Kedar Challakere | Stem cell enhanced protein products and uses thereof |
| US20110091604A1 (en) | 2009-10-21 | 2011-04-21 | Seth Adrian Miller | Synthetic meat |
| WO2012034101A2 (en) | 2010-09-09 | 2012-03-15 | The Regents Of The University Of California | Expandable cell source of neuronal stem cell populations and methods for obtaining and using them |
| BR112013031770A2 (pt) * | 2011-06-10 | 2016-12-06 | Univ Georgia | população de células-tronco aviárias de pluripotência induzida, ave quimérica, método de reprogamação de uma célula embrionária aviária ou célula somática adulta, método de manipulação genética de espécies aviárias, método de instilar a resistência à doença em uma célula-tronco aviária pluripotente induzida, método de instilar a resistência à doença em uma população de célula-tronco aviária pluripotente, método para produzir um gameta de ave quimérica, método de produção de material imunogênico para uma vacina e vacina |
| US8900572B2 (en) * | 2011-06-14 | 2014-12-02 | UNIVERSITé LAVAL | Myogenic differentiation of stem cells and uses thereof |
| US8883503B2 (en) | 2011-06-23 | 2014-11-11 | Indian Institute Of Technology Kanpur | Hydrogel scaffolds for tissue engineering |
| US10039306B2 (en) | 2012-03-16 | 2018-08-07 | Impossible Foods Inc. | Methods and compositions for consumables |
| BR112014000614A2 (pt) | 2011-07-12 | 2017-07-11 | Maraxi Inc | métodos e composições para consumíveis |
| PL2736357T3 (pl) | 2011-07-26 | 2019-02-28 | The Curators Of The University Of Missouri | Poddawane inżynierii jadalne mięso |
| CA2792081C (en) * | 2011-10-11 | 2020-10-27 | Bond University Ltd | Layered compositions comprising 3d nanofibre webbing for tissue repair |
| JP6083877B2 (ja) * | 2011-11-18 | 2017-02-22 | 国立大学法人京都大学 | 多能性幹細胞から骨格筋細胞への分化誘導方法 |
| US9737875B2 (en) | 2012-03-16 | 2017-08-22 | Impossible Foods Inc. | Affinity reagents for protein purification |
| CN104603293A (zh) | 2012-03-28 | 2015-05-06 | 现代牧场有限公司 | 工程的皮革和其制造方法 |
| RU2672489C2 (ru) | 2013-01-11 | 2018-11-15 | Импоссибл Фудз Инк. | Немолочный аналог сыра, содержащий коацерват |
| EP2959005B1 (en) | 2013-02-22 | 2021-10-06 | The Board of Trustees of the Leland Stanford Junior University | Medical use relating to telomere extension |
| WO2015038988A1 (en) | 2013-09-13 | 2015-03-19 | Modern Meadow, Inc. | Edible and animal-product-free microcarriers for engineered meat |
| BR112016009803B1 (pt) | 2013-10-30 | 2022-02-08 | The Curators Of The University Of Missouri | Método para especificação e cultivo de linhagem muscular esquelética escalonável |
| US9526267B2 (en) | 2014-04-17 | 2016-12-27 | Savage River, Inc. | Nutrient-dense meat structured protein products |
| US20150296834A1 (en) | 2014-04-17 | 2015-10-22 | Savage River, Inc. dba Beyond Meat, Inc. | Plant based meat structured protein products |
| WO2017019125A1 (en) | 2015-07-24 | 2017-02-02 | Synthetic Genomics, Inc. | A protein rich food ingredient from biomass and methods of production |
| CA3011484A1 (en) | 2016-01-14 | 2017-07-20 | Memphis Meats, Inc. | Methods for extending the replicative capacity of somatic cells during an ex vivo cultivation process |
| ES2985769T3 (es) | 2017-05-06 | 2024-11-07 | Upside Foods Inc | Composiciones y métodos para aumentar la densidad de cultivo de una biomasa celular dentro de una infraestructura de cultivo |
| WO2019014652A1 (en) | 2017-07-13 | 2019-01-17 | Memphis Meats, Inc. | COMPOSITIONS AND METHODS FOR INCREASING THE EFFICACY OF CELL CULTURES USED IN FOOD PRODUCTION |
-
2014
- 2014-10-30 BR BR112016009803-0A patent/BR112016009803B1/pt active IP Right Grant
- 2014-10-30 US US15/033,207 patent/US20160251625A1/en not_active Abandoned
- 2014-10-30 IL IL296889A patent/IL296889A/en unknown
- 2014-10-30 JP JP2016552246A patent/JP6728049B2/ja active Active
- 2014-10-30 CA CA2928726A patent/CA2928726A1/en active Pending
- 2014-10-30 WO PCT/US2014/063250 patent/WO2015066377A1/en not_active Ceased
- 2014-10-30 EP EP14858383.4A patent/EP3071040B2/en active Active
- 2014-10-30 AU AU2014342180A patent/AU2014342180B2/en not_active Ceased
- 2014-10-30 ES ES14858383T patent/ES2782380T5/es active Active
- 2014-10-30 CN CN202010084479.7A patent/CN111154726A/zh active Pending
- 2014-10-30 CN CN201480061537.7A patent/CN105899079A/zh not_active Withdrawn
-
2016
- 2016-04-20 US US15/134,252 patent/US10920196B2/en active Active
- 2016-04-24 IL IL245292A patent/IL245292B2/en unknown
-
2019
- 2019-04-02 AU AU2019202244A patent/AU2019202244B2/en not_active Ceased
-
2020
- 2020-01-17 JP JP2020005873A patent/JP2020089381A/ja active Pending
-
2021
- 2021-02-12 US US17/175,324 patent/US20210171912A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| JP6728049B2 (ja) | 2020-07-22 |
| EP3071040B1 (en) | 2020-01-01 |
| ES2782380T3 (es) | 2020-09-14 |
| BR112016009803B1 (pt) | 2022-02-08 |
| CN111154726A (zh) | 2020-05-15 |
| EP3071040B2 (en) | 2022-12-28 |
| CA2928726A1 (en) | 2015-05-07 |
| AU2019202244A1 (en) | 2019-04-18 |
| IL245292A0 (en) | 2016-06-30 |
| AU2019202244B2 (en) | 2021-09-23 |
| IL245292B (en) | 2022-10-01 |
| US20160251625A1 (en) | 2016-09-01 |
| IL245292B2 (en) | 2023-02-01 |
| AU2014342180A1 (en) | 2016-05-19 |
| US20210171912A1 (en) | 2021-06-10 |
| CN105899079A (zh) | 2016-08-24 |
| EP3071040A1 (en) | 2016-09-28 |
| BR112016009803A2 (pt) | 2017-12-05 |
| EP3071040A4 (en) | 2017-06-21 |
| US10920196B2 (en) | 2021-02-16 |
| JP2020089381A (ja) | 2020-06-11 |
| ES2782380T5 (es) | 2023-05-08 |
| WO2015066377A1 (en) | 2015-05-07 |
| AU2014342180B2 (en) | 2019-01-03 |
| US20160227830A1 (en) | 2016-08-11 |
| BR112016009803A8 (pt) | 2018-01-30 |
| JP2016535601A (ja) | 2016-11-17 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| IL296889A (en) | A method for breeding and genealogical description of quantitative skeletal muscle | |
| Ren et al. | Maintenance of nucleolar homeostasis by CBX4 alleviates senescence and osteoarthritis | |
| Xu et al. | A zebrafish embryo culture system defines factors that promote vertebrate myogenesis across species | |
| EP3059308B1 (en) | Method of inducing kidney from pluripotent stem cells | |
| KR20160029115A (ko) | 비-다능성 세포를 다능성 줄기 세포가 되도록 재프로그래밍하기 위한 조성물 및 방법 | |
| EP2986715A1 (en) | Media compositions for neuronal cell culture | |
| WO2011004911A1 (en) | Method of inducing differentiation from pluripotent stem cells to skeletal muscle progenitor cells | |
| WO2013109763A2 (en) | Activation of innate immunity for nuclear reprogramming of somatic cells | |
| US11697798B2 (en) | Enhanced differentiation and maturation of pluripotent stem cell-derived myogenic cells | |
| US20240279613A1 (en) | Method of producing enteroendocrine cell, enteroendocrine cell derived from pluripotent stem cell, culture medium or culture medium kit, and use thereof | |
| US20240409903A1 (en) | Method of differentiating human pluripotent stem cells to podocytes | |
| US12054742B2 (en) | Compositions and methods to derive mesodermal lineage cells and mixed tissue organoids from embryonic stem cells | |
| Wang et al. | Characterization of embryonic stem-like cells derived from HEK293T cells through miR302/367 expression and their potentiality to differentiate into germ-like cells | |
| HK1229164A1 (en) | Method for scalable skeletal muscle lineage specification and cultivation | |
| HK1229164B (en) | Method for scalable skeletal muscle lineage specification and cultivation | |
| Shahriyari et al. | Human engineered skeletal muscle of hypaxial origin from pluripotent stem cells with advanced function and regenerative capacity | |
| Falco | Reprogramación directa de fibroblastos humanos para modelar la esclerosis lateral amiotrófica in vitro | |
| Bullen | An interrogation into the role of p62 in the pluripotent state | |
| Corbin | COMPLEXATION OF LIPIDS WITH CYCLODEXTRIN CARRIERS FOR FULLY DEFINED SUPPLEMENTATION OF CELL CULTURE | |
| Page et al. | Methods for Inducing Pluripotency | |
| Crossley | Modelling Neuromuscular Circuits in Vitro | |
| Robertson | Targeting the Hippo signalling pathway to enhance the protective effect of iPS cell derived cardiomyocytes |