MX2018008733A - Metodos para extender la capacidad replicativa de las celulas somaticas durante un proceso de cultivo ex vivo. - Google Patents

Metodos para extender la capacidad replicativa de las celulas somaticas durante un proceso de cultivo ex vivo.

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Publication number
MX2018008733A
MX2018008733A MX2018008733A MX2018008733A MX2018008733A MX 2018008733 A MX2018008733 A MX 2018008733A MX 2018008733 A MX2018008733 A MX 2018008733A MX 2018008733 A MX2018008733 A MX 2018008733A MX 2018008733 A MX2018008733 A MX 2018008733A
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Mexico
Prior art keywords
amendments
extending
replicative
somatic cells
replicative capacity
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MX2018008733A
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English (en)
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Genovese Nicholas
Nicole Desmet Danielle
Schulze Eric
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Memphis Meats Inc
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Application filed by Memphis Meats Inc filed Critical Memphis Meats Inc
Publication of MX2018008733A publication Critical patent/MX2018008733A/es

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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
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    • C12N5/0602—Vertebrate cells
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Abstract

Un producto y proceso para extender la capacidad replicativa de las células somáticas metazoas usando enmiendas genéticas dirigidas para anular la inhibición de la progresión del ciclo celular durante la senescencia replicativa y derivar líneas celulares clonales para aplicaciones escalables y producción industrial de biomasa de células metazoas. Se crea una mutación de inserción o deleción utilizando ARN guía dirigidos al primer exón de la transcripción que codifica cada proteína usando CRISPR/Cas9. Las enmiendas dirigidas dan como resultado la inactivación de las proteínas p15 y p16, lo que aumenta la capacidad proliferativa de las poblaciones de células modificadas en relación con sus poblaciones parentales no alteradas. La combinación de estas enmiendas con la actividad de la telomerasa auxiliar de una construcción genética que dirige la expresión de un homólogo de la proteína de la telomerasa a partir de un gen TERT, aumenta la capacidad replicativa de las poblaciones de células modificadas de forma indefinida. Una aplicación es fabricar músculo esquelético para consumo dietético usando células de la especie aviar Gallus gallus; y otro es de la especie de ganado Bos taurus.
MX2018008733A 2016-01-14 2017-01-17 Metodos para extender la capacidad replicativa de las celulas somaticas durante un proceso de cultivo ex vivo. MX2018008733A (es)

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US201662278869P 2016-01-14 2016-01-14
US201662361867P 2016-07-13 2016-07-13
PCT/US2017/013782 WO2017124100A1 (en) 2016-01-14 2017-01-17 Methods for extending the replicative capacity of somatic cells during an ex vivo cultivation process

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US (2) US20190024079A1 (es)
EP (1) EP3394246A4 (es)
JP (1) JP2019501657A (es)
KR (1) KR20180134847A (es)
CN (1) CN108779471A (es)
AU (1) AU2017208094A1 (es)
CA (1) CA3011484A1 (es)
MX (1) MX2018008733A (es)
SG (1) SG11201806002SA (es)
WO (1) WO2017124100A1 (es)

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CN108779471A (zh) 2018-11-09
WO2017124100A1 (en) 2017-07-20
US20190024079A1 (en) 2019-01-24
EP3394246A4 (en) 2019-05-22
KR20180134847A (ko) 2018-12-19
US20220251550A1 (en) 2022-08-11
CA3011484A1 (en) 2017-07-20
JP2019501657A (ja) 2019-01-24
AU2017208094A1 (en) 2018-08-09
EP3394246A1 (en) 2018-10-31
SG11201806002SA (en) 2018-08-30

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