IL282517B - Overexpression of n-glycosylation pathway regulators to modulate glycosylation of recombinant proteins - Google Patents

Overexpression of n-glycosylation pathway regulators to modulate glycosylation of recombinant proteins

Info

Publication number
IL282517B
IL282517B IL282517A IL28251721A IL282517B IL 282517 B IL282517 B IL 282517B IL 282517 A IL282517 A IL 282517A IL 28251721 A IL28251721 A IL 28251721A IL 282517 B IL282517 B IL 282517B
Authority
IL
Israel
Prior art keywords
cell
cells
culture
protein
host cell
Prior art date
Application number
IL282517A
Other languages
English (en)
Hebrew (he)
Other versions
IL282517A (en
Original Assignee
Amgen Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=53757626&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=IL282517(B) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Amgen Inc filed Critical Amgen Inc
Publication of IL282517A publication Critical patent/IL282517A/en
Publication of IL282517B publication Critical patent/IL282517B/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/005—Glycopeptides, glycoproteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01094—Protein N-acetylglucosaminyltransferase (2.4.1.94)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01101—Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase (2.4.1.101)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01143—Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase (2.4.1.143)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/40—Immunoglobulins specific features characterized by post-translational modification
    • C07K2317/41—Glycosylation, sialylation, or fucosylation

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Saccharide Compounds (AREA)
IL282517A 2014-01-29 2014-12-11 Overexpression of n-glycosylation pathway regulators to modulate glycosylation of recombinant proteins IL282517B (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201461933137P 2014-01-29 2014-01-29
PCT/US2014/069744 WO2015116315A1 (en) 2014-01-29 2014-12-11 Overexpression of n-glycosylation pathway regulators to modulate glycosylation of recombinant proteins

Publications (2)

Publication Number Publication Date
IL282517A IL282517A (en) 2021-06-30
IL282517B true IL282517B (en) 2022-07-01

Family

ID=53757626

Family Applications (3)

Application Number Title Priority Date Filing Date
IL282517A IL282517B (en) 2014-01-29 2014-12-11 Overexpression of n-glycosylation pathway regulators to modulate glycosylation of recombinant proteins
IL246914A IL246914B (en) 2014-01-29 2016-07-24 Overexpression of n-glycosylation pathway regulators to modulate glycosylation of recombinant proteins
IL270861A IL270861B (en) 2014-01-29 2019-11-24 Overexpression of n-glycosylation pathway regulators to modulate glycosylation of recombinant proteins

Family Applications After (2)

Application Number Title Priority Date Filing Date
IL246914A IL246914B (en) 2014-01-29 2016-07-24 Overexpression of n-glycosylation pathway regulators to modulate glycosylation of recombinant proteins
IL270861A IL270861B (en) 2014-01-29 2019-11-24 Overexpression of n-glycosylation pathway regulators to modulate glycosylation of recombinant proteins

Country Status (14)

Country Link
US (3) US10227627B2 (cg-RX-API-DMAC7.html)
EP (1) EP3099806A1 (cg-RX-API-DMAC7.html)
JP (4) JP6474420B2 (cg-RX-API-DMAC7.html)
KR (3) KR102410393B1 (cg-RX-API-DMAC7.html)
CN (2) CN106170554B (cg-RX-API-DMAC7.html)
AU (3) AU2014380174C1 (cg-RX-API-DMAC7.html)
BR (1) BR112016017606A2 (cg-RX-API-DMAC7.html)
CA (1) CA2938079C (cg-RX-API-DMAC7.html)
CL (1) CL2016001900A1 (cg-RX-API-DMAC7.html)
EA (2) EA037565B1 (cg-RX-API-DMAC7.html)
IL (3) IL282517B (cg-RX-API-DMAC7.html)
MX (2) MX377780B (cg-RX-API-DMAC7.html)
SG (1) SG10201912081RA (cg-RX-API-DMAC7.html)
WO (1) WO2015116315A1 (cg-RX-API-DMAC7.html)

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KR101936049B1 (ko) 2015-10-15 2019-01-08 (주)알테오젠 IgG Fc 도메인을 가지는 융합 단백질의 생산방법
EP3596225A1 (en) * 2017-03-14 2020-01-22 Amgen Inc. Control of total afucosylated glycoforms of antibodies produced in cell culture
IL321253A (en) 2018-05-01 2025-08-01 Amgen Inc Antibodies with a modulated glycan profile
CN109576212B (zh) * 2018-12-14 2021-06-22 杭州奕安济世生物药业有限公司 高密度接种培养中种子细胞的培养方法及其应用
KR102648479B1 (ko) * 2019-06-10 2024-03-15 초 플러스 인크. 단백질 기반 의약들 (protein-based pharmaceuticals) 의 고 수준 생산을 위한 세포주들 (cell lines)
BR112023021867A2 (pt) 2021-04-23 2023-12-19 Amgen Inc Composições de anticorpo anti-tslp e seus usos
TW202309087A (zh) * 2021-05-03 2023-03-01 德商百靈佳殷格翰國際股份有限公司 製備spesolimab之方法
CN113684322B (zh) * 2021-09-09 2024-01-23 上海药明生物技术有限公司 一种降低抗体类蛋白高聚甘露糖型水平的方法
CN114525252B (zh) * 2022-03-10 2023-12-01 广州源井生物科技有限公司 一种提高mda-mb-231单细胞克隆形成率的单克隆增强培养基与培养方法及其应用
UY40500A (es) 2022-10-26 2024-04-30 Amgen Inc Composiciones de anticuerpos anti-tslp y usos de las mismas

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WO2011134643A1 (en) * 2010-04-27 2011-11-03 Lonza Ltd. Improved glycosylation of proteins in host cells

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WO2011134643A1 (en) * 2010-04-27 2011-11-03 Lonza Ltd. Improved glycosylation of proteins in host cells
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Also Published As

Publication number Publication date
AU2014380174A1 (en) 2016-08-11
AU2018264100A1 (en) 2018-12-06
JP2017505620A (ja) 2017-02-23
BR112016017606A2 (pt) 2017-10-10
JP7788307B2 (ja) 2025-12-18
EA037565B1 (ru) 2021-04-14
US10655156B2 (en) 2020-05-19
CA2938079C (en) 2022-10-11
JP2022068365A (ja) 2022-05-09
MX377780B (es) 2025-03-11
CN106170554B (zh) 2021-08-17
SG10201912081RA (en) 2020-02-27
US20200277642A1 (en) 2020-09-03
KR20160111943A (ko) 2016-09-27
KR102301034B1 (ko) 2021-09-13
IL246914A0 (en) 2016-09-29
IL270861B (en) 2021-05-31
KR20220086703A (ko) 2022-06-23
CN106170554A (zh) 2016-11-30
IL270861A (en) 2020-01-30
MX2020012356A (es) 2021-01-29
US20190153496A1 (en) 2019-05-23
AU2018264100C1 (en) 2025-06-05
AU2020239681C1 (en) 2025-06-05
WO2015116315A1 (en) 2015-08-06
JP6474420B2 (ja) 2019-02-27
JP2021040638A (ja) 2021-03-18
CN113774084B (zh) 2024-04-16
AU2018264100B2 (en) 2020-07-23
EP3099806A1 (en) 2016-12-07
AU2014380174C1 (en) 2025-06-05
CN113774084A (zh) 2021-12-10
JP6797950B2 (ja) 2020-12-09
AU2020239681A1 (en) 2020-10-15
IL282517A (en) 2021-06-30
CA2938079A1 (en) 2015-08-06
EA202190244A3 (ru) 2021-08-31
US20190010532A1 (en) 2019-01-10
KR102519540B1 (ko) 2023-04-10
US10907186B2 (en) 2021-02-02
MX2016009911A (es) 2016-10-28
JP2019088305A (ja) 2019-06-13
EA201691532A1 (ru) 2017-01-30
IL246914B (en) 2019-12-31
AU2020239681B2 (en) 2022-06-23
CL2016001900A1 (es) 2017-05-12
EA202190244A2 (ru) 2021-05-31
AU2014380174B2 (en) 2018-08-30
KR102410393B1 (ko) 2022-06-16
JP7034236B2 (ja) 2022-03-11
US10227627B2 (en) 2019-03-12
KR20210114549A (ko) 2021-09-23

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