IL280772B2 - Multiplex nucleic acid testing - Google Patents

Multiplex nucleic acid testing

Info

Publication number
IL280772B2
IL280772B2 IL280772A IL28077221A IL280772B2 IL 280772 B2 IL280772 B2 IL 280772B2 IL 280772 A IL280772 A IL 280772A IL 28077221 A IL28077221 A IL 28077221A IL 280772 B2 IL280772 B2 IL 280772B2
Authority
IL
Israel
Prior art keywords
loop
stem
target
oligonucleotide
closed
Prior art date
Application number
IL280772A
Other languages
English (en)
Hebrew (he)
Other versions
IL280772A (en
IL280772B1 (en
Inventor
Jane HASICK Nicole
Rae Kim Ryung
Lee LAWRENCE Andrea
Velyian Todd Alison
Original Assignee
Speedx Pty Ltd
Jane HASICK Nicole
Rae Kim Ryung
Lee LAWRENCE Andrea
Velyian Todd Alison
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from AU2018902915A external-priority patent/AU2018902915A0/en
Application filed by Speedx Pty Ltd, Jane HASICK Nicole, Rae Kim Ryung, Lee LAWRENCE Andrea, Velyian Todd Alison filed Critical Speedx Pty Ltd
Publication of IL280772A publication Critical patent/IL280772A/en
Publication of IL280772B1 publication Critical patent/IL280772B1/en
Publication of IL280772B2 publication Critical patent/IL280772B2/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/30—Phosphoric diester hydrolysing, i.e. nuclease
    • C12Q2521/345—DNAzyme
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/30—Oligonucleotides characterised by their secondary structure
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/30—Oligonucleotides characterised by their secondary structure
    • C12Q2525/301—Hairpin oligonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/101—Temperature
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
    • C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
    • C12Q2537/143—Multiplexing, i.e. use of multiple primers or probes in a single reaction, usually for simultaneously analyse of multiple analysis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/107—Nucleic acid detection characterized by the use of physical, structural and functional properties fluorescence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/10—Detection mode being characterised by the assay principle
    • C12Q2565/101—Interaction between at least two labels
    • C12Q2565/1015—Interaction between at least two labels labels being on the same oligonucleotide
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6432—Quenching
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Optics & Photonics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
IL280772A 2018-08-09 2019-08-09 Multiplex nucleic acid testing IL280772B2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
AU2018902915A AU2018902915A0 (en) 2018-08-09 Multiplex detection of nucleic acids
PCT/IB2019/056811 WO2020031156A1 (en) 2018-08-09 2019-08-09 Multiplex detection of nucleic acids

Publications (3)

Publication Number Publication Date
IL280772A IL280772A (en) 2021-04-29
IL280772B1 IL280772B1 (en) 2024-12-01
IL280772B2 true IL280772B2 (en) 2025-04-01

Family

ID=69413249

Family Applications (1)

Application Number Title Priority Date Filing Date
IL280772A IL280772B2 (en) 2018-08-09 2019-08-09 Multiplex nucleic acid testing

Country Status (12)

Country Link
US (1) US12163182B2 (enExample)
EP (1) EP3833778A4 (enExample)
JP (1) JP7423603B2 (enExample)
KR (1) KR102796204B1 (enExample)
CN (1) CN112823212B (enExample)
AU (1) AU2019316815B2 (enExample)
BR (1) BR112021002398A2 (enExample)
CA (1) CA3108430A1 (enExample)
IL (1) IL280772B2 (enExample)
MX (1) MX2021001385A (enExample)
SG (1) SG11202101140TA (enExample)
WO (1) WO2020031156A1 (enExample)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SG11202101140TA (en) 2018-08-09 2021-03-30 Speedx Pty Ltd Multiplex detection of nucleic acids
CN116018414A (zh) * 2020-06-30 2023-04-25 斯皮德斯私人有限公司 使用混合报告子的多重核酸检测
CN114941021B (zh) * 2022-05-12 2026-06-02 陕西师范大学 基于探针连接的miRNAs定量检测试剂盒及检测方法
KR20240028084A (ko) 2022-08-24 2024-03-05 주식회사 멀티렉스 표지자가 결합된 태그 프로브 및 이의 용도
CN120019163A (zh) * 2022-09-07 2025-05-16 贝克顿迪金森公司 古细菌聚合酶扩增
JP2025541744A (ja) * 2022-12-01 2025-12-23 ベクトン・ディキンソン・アンド・カンパニー 核酸検出のための非対称性ヘアピンプローブ
EP4665870A1 (en) * 2023-02-16 2025-12-24 Asecud SA High nucleic acid sequence discrimination using a multifaceted nucleic acid molecule

Family Cites Families (16)

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US5514546A (en) * 1993-09-01 1996-05-07 Research Corporation Technologies, Inc. Stem-loop oligonucleotides containing parallel and antiparallel binding domains
CN1327070A (zh) * 2000-06-03 2001-12-19 庄臣及庄臣研究股份有限公司 以具有催化活性的核酸为基础的诊断方法
DK1948822T3 (da) 2005-10-07 2011-10-17 Johnson & Johnson Res Pty Ltd Multikomponent-nukleinsyreenzymer og fremgangsmåder til deres anvendelse
MX2009003193A (es) * 2006-10-06 2009-08-13 Johnson & Johnson Res Pty Ltd Interruptores moleculares y metodos para su uso.
WO2008122084A1 (en) 2007-04-05 2008-10-16 Johnson & Johnson Research Pty Limited Nucleic acid enzymes and complexes and methods for their use
CN101241097B (zh) * 2007-09-18 2011-08-03 中国科学院上海应用物理研究所 一种采用茎环结构检测探针的电化学dna检测方法及其试剂盒
GB201017978D0 (en) * 2010-10-25 2010-12-08 Oxitec Ltd Multiplex amplification and detection
WO2012159089A1 (en) * 2011-05-19 2012-11-22 Sequenom, Inc. Products and processes for multiplex nucleic acid identification
NO2817421T3 (enExample) * 2012-02-20 2018-05-12
CN102559918B (zh) * 2012-02-28 2014-10-01 盛司潼 一种利用酶切延伸增加读长的测序方法
JP5958034B2 (ja) * 2012-04-11 2016-07-27 富士レビオ株式会社 分子ビーコン型プローブを用いた標的核酸の検出方法
AR091774A1 (es) * 2012-07-16 2015-02-25 Dow Agrosciences Llc Proceso para el diseño de las secuencias de adn repetidas, largas, divergentes de codones optimizados
EP4697022A3 (en) 2013-08-09 2026-06-03 Luminex Corporation Probes for improved melt discrimination and multiplexing in nucleic acid assays
EP3180449B1 (en) * 2014-08-11 2019-10-09 Luminex Corporation Probes for improved melt discrimination and multiplexing in nucleic acid assays
DK3512959T3 (da) 2016-09-15 2021-10-18 Hoffmann La Roche Fremgangsmåder til udførelse af multipleks PCR
SG11202101140TA (en) 2018-08-09 2021-03-30 Speedx Pty Ltd Multiplex detection of nucleic acids

Also Published As

Publication number Publication date
CA3108430A1 (en) 2020-02-13
EP3833778A4 (en) 2022-12-07
US20220056507A1 (en) 2022-02-24
CN112823212B (zh) 2025-09-02
NZ772437A (en) 2025-08-29
EP3833778A1 (en) 2021-06-16
WO2020031156A1 (en) 2020-02-13
SG11202101140TA (en) 2021-03-30
AU2019316815B2 (en) 2026-01-08
JP7423603B2 (ja) 2024-01-29
MX2021001385A (es) 2021-06-23
KR20210057028A (ko) 2021-05-20
IL280772A (en) 2021-04-29
JP2021533763A (ja) 2021-12-09
US12163182B2 (en) 2024-12-10
KR102796204B1 (ko) 2025-04-14
CN112823212A (zh) 2021-05-18
IL280772B1 (en) 2024-12-01
AU2019316815A1 (en) 2021-02-18
BR112021002398A2 (pt) 2021-05-04

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