IL236814A0 - The assembly of DNA segments in high yield - Google Patents

The assembly of DNA segments in high yield

Info

Publication number
IL236814A0
IL236814A0 IL236814A IL23681415A IL236814A0 IL 236814 A0 IL236814 A0 IL 236814A0 IL 236814 A IL236814 A IL 236814A IL 23681415 A IL23681415 A IL 23681415A IL 236814 A0 IL236814 A0 IL 236814A0
Authority
IL
Israel
Prior art keywords
dna fragment
fragment assembly
throughput dna
throughput
assembly
Prior art date
Application number
IL236814A
Other languages
English (en)
Hebrew (he)
Inventor
Gupta Manju
L Evans Steven
Sandeep Kumar
Original Assignee
Dow Agrosciences Llc
Gupta Manju
L Evans Steven
Sandeep Kumar
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dow Agrosciences Llc, Gupta Manju, L Evans Steven, Sandeep Kumar filed Critical Dow Agrosciences Llc
Publication of IL236814A0 publication Critical patent/IL236814A0/en

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67General methods for enhancing the expression
    • C12N15/68Stabilisation of the vector
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8209Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Enzymes And Modification Thereof (AREA)
IL236814A 2012-07-26 2015-01-20 The assembly of DNA segments in high yield IL236814A0 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201261675929P 2012-07-26 2012-07-26
PCT/US2013/051641 WO2014018512A1 (en) 2012-07-26 2013-07-23 High-throughput dna fragment assembly

Publications (1)

Publication Number Publication Date
IL236814A0 true IL236814A0 (en) 2015-03-31

Family

ID=48914467

Family Applications (1)

Application Number Title Priority Date Filing Date
IL236814A IL236814A0 (en) 2012-07-26 2015-01-20 The assembly of DNA segments in high yield

Country Status (13)

Country Link
US (1) US9752154B2 (cg-RX-API-DMAC10.html)
EP (1) EP2877581B1 (cg-RX-API-DMAC10.html)
JP (1) JP2015525567A (cg-RX-API-DMAC10.html)
KR (1) KR20150032306A (cg-RX-API-DMAC10.html)
CN (1) CN104619847A (cg-RX-API-DMAC10.html)
AU (1) AU2013293123B2 (cg-RX-API-DMAC10.html)
BR (1) BR112015001316A2 (cg-RX-API-DMAC10.html)
CA (1) CA2882143A1 (cg-RX-API-DMAC10.html)
IL (1) IL236814A0 (cg-RX-API-DMAC10.html)
IN (1) IN2014DN11250A (cg-RX-API-DMAC10.html)
RU (1) RU2015106549A (cg-RX-API-DMAC10.html)
WO (1) WO2014018512A1 (cg-RX-API-DMAC10.html)
ZA (1) ZA201409509B (cg-RX-API-DMAC10.html)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107002150B (zh) * 2015-01-09 2020-09-01 深圳华大基因研究院 一种dna合成产物的高通量检测方法
CN106318961B (zh) * 2015-06-30 2019-07-09 未名生物农业集团有限公司 一种多dna片段载体的构建方法及其应用

Family Cites Families (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4535060A (en) 1983-01-05 1985-08-13 Calgene, Inc. Inhibition resistant 5-enolpyruvyl-3-phosphoshikimate synthetase, production and use
US5380831A (en) 1986-04-04 1995-01-10 Mycogen Plant Science, Inc. Synthetic insecticidal crystal protein gene
US4761373A (en) 1984-03-06 1988-08-02 Molecular Genetics, Inc. Herbicide resistance in plants
US4940835A (en) 1985-10-29 1990-07-10 Monsanto Company Glyphosate-resistant plants
AU611080B2 (en) 1986-01-08 1991-06-06 Rhone-Poulenc Agrochimie Haloarylnitrile degrading gene, its use, and cells containing the same
US4810648A (en) 1986-01-08 1989-03-07 Rhone Poulenc Agrochimie Haloarylnitrile degrading gene, its use, and cells containing the gene
EP0242236B2 (en) 1986-03-11 1996-08-21 Plant Genetic Systems N.V. Plant cells resistant to glutamine synthetase inhibitors, made by genetic engineering
US4975374A (en) 1986-03-18 1990-12-04 The General Hospital Corporation Expression of wild type and mutant glutamine synthetase in foreign hosts
EP0333033A1 (en) 1988-03-09 1989-09-20 Meiji Seika Kaisha Ltd. Glutamine synthesis gene and glutamine synthetase
EP0528819A1 (en) 1990-04-18 1993-03-03 Plant Genetic Systems, N.V. Modified bacillus thuringiensis insecticidal-crystal protein genes and their expression in plant cells
US5633435A (en) 1990-08-31 1997-05-27 Monsanto Company Glyphosate-tolerant 5-enolpyruvylshikimate-3-phosphate synthases
TW261517B (cg-RX-API-DMAC10.html) 1991-11-29 1995-11-01 Mitsubishi Shozi Kk
US7351578B2 (en) * 1999-12-10 2008-04-01 Invitrogen Corp. Use of multiple recombination sites with unique specificity in recombinational cloning
US8877916B2 (en) * 2000-04-26 2014-11-04 Ceres, Inc. Promoter, promoter control elements, and combinations, and uses thereof
JP4394878B2 (ja) * 2000-12-08 2010-01-06 ライフ テクノロジーズ コーポレーション 複数の認識部位を用いた核酸分子合成のための方法および組成物
JP2007500514A (ja) 2003-04-29 2007-01-18 パイオニア ハイ−ブレッド インターナショナル, インコーポレイテッド 新規なグリホセートn−アセチルトランスフェラーゼ(gat)遺伝子
WO2004098530A2 (en) * 2003-05-05 2004-11-18 Dow Agrosciences Llc Stable immunoprophylactic and therapeutic compositions derived from transgenic plant cells and methods for production
DK1740039T3 (da) 2004-04-30 2012-08-20 Dow Agrosciences Llc Hidtil ukendte herbicidresistensgener
CA2628263C (en) 2005-10-28 2016-12-13 Dow Agrosciences Llc Aryloxyalkanoate auxin herbicide resistance genes and uses thereof

Also Published As

Publication number Publication date
BR112015001316A2 (pt) 2017-08-01
WO2014018512A1 (en) 2014-01-30
CN104619847A (zh) 2015-05-13
EP2877581A1 (en) 2015-06-03
US9752154B2 (en) 2017-09-05
CA2882143A1 (en) 2014-01-30
AU2013293123B2 (en) 2018-04-05
JP2015525567A (ja) 2015-09-07
US20150176015A1 (en) 2015-06-25
ZA201409509B (en) 2016-08-31
RU2015106549A (ru) 2016-09-20
AU2013293123A1 (en) 2015-01-22
KR20150032306A (ko) 2015-03-25
IN2014DN11250A (cg-RX-API-DMAC10.html) 2015-10-09
EP2877581B1 (en) 2017-11-29

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