IL212387A0 - Perfusion bioreactors, cell culture systems, and methods for production of cells and cell-derived products - Google Patents

Perfusion bioreactors, cell culture systems, and methods for production of cells and cell-derived products

Info

Publication number
IL212387A0
IL212387A0 IL212387A IL21238711A IL212387A0 IL 212387 A0 IL212387 A0 IL 212387A0 IL 212387 A IL212387 A IL 212387A IL 21238711 A IL21238711 A IL 21238711A IL 212387 A0 IL212387 A0 IL 212387A0
Authority
IL
Israel
Prior art keywords
cell
cells
production
methods
culture systems
Prior art date
Application number
IL212387A
Other languages
English (en)
Original Assignee
Biovest Int Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biovest Int Inc filed Critical Biovest Int Inc
Publication of IL212387A0 publication Critical patent/IL212387A0/en

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/42Integrated assemblies, e.g. cassettes or cartridges
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P17/00Drugs for dermatological disorders
    • A61P17/02Drugs for dermatological disorders for treating wounds, ulcers, burns, scars, keloids, or the like
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P19/00Drugs for skeletal disorders
    • A61P19/02Drugs for skeletal disorders for joint disorders, e.g. arthritis, arthrosis
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P21/00Drugs for disorders of the muscular or neuromuscular system
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12Antivirals
    • A61P31/14Antivirals for RNA viruses
    • A61P31/16Antivirals for RNA viruses for influenza or rhinoviruses
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/28Constructional details, e.g. recesses, hinges disposable or single use
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/44Multiple separable units; Modules
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M25/00Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/14Scaffolds; Matrices
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/10Perfusion

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Sustainable Development (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Clinical Laboratory Science (AREA)
  • Veterinary Medicine (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Public Health (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Animal Behavior & Ethology (AREA)
  • Virology (AREA)
  • Immunology (AREA)
  • Orthopedic Medicine & Surgery (AREA)
  • Physical Education & Sports Medicine (AREA)
  • Molecular Biology (AREA)
  • Dermatology (AREA)
  • Rheumatology (AREA)
  • Neurology (AREA)
  • Oncology (AREA)
  • Communicable Diseases (AREA)
  • Pulmonology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
IL212387A 2008-10-22 2011-04-14 Perfusion bioreactors, cell culture systems, and methods for production of cells and cell-derived products IL212387A0 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US10764408P 2008-10-22 2008-10-22
PCT/US2009/061700 WO2010048417A2 (en) 2008-10-22 2009-10-22 Perfusion bioreactors, cell culture systems, and methods for production of cells and cell-derived products

Publications (1)

Publication Number Publication Date
IL212387A0 true IL212387A0 (en) 2011-06-30

Family

ID=42119984

Family Applications (1)

Application Number Title Priority Date Filing Date
IL212387A IL212387A0 (en) 2008-10-22 2011-04-14 Perfusion bioreactors, cell culture systems, and methods for production of cells and cell-derived products

Country Status (7)

Country Link
US (1) US20110212493A1 (https=)
EP (1) EP2346984A4 (https=)
JP (1) JP2012506257A (https=)
AU (1) AU2009308354A1 (https=)
CA (1) CA2741481A1 (https=)
IL (1) IL212387A0 (https=)
WO (1) WO2010048417A2 (https=)

Families Citing this family (70)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2029722B1 (en) 2006-05-22 2019-10-16 Biovest International, Inc. Method and system for the production of cells
EP2609189A4 (en) * 2010-08-24 2015-08-05 Hawley & Hazel Chemical Co Zhongshan Ltd METHODS, DEVICES AND USES RELATED TO BIOFILMS
WO2012048276A2 (en) 2010-10-08 2012-04-12 Caridianbct, Inc. Customizable methods and systems of growing and harvesting cells in a hollow fiber bioreactor system
US9220810B2 (en) * 2010-12-10 2015-12-29 Florida State University Research Foundation, Inc. Mesenchymal stem cells (MSC) expansion methods and materials
WO2012163368A1 (en) * 2011-05-30 2012-12-06 Atech System Solutions Ltd. Bioreactor system for continuous cell cultivation
WO2012171026A2 (en) * 2011-06-10 2012-12-13 Biovest International, Inc. Methods for high yield virus production
WO2012171030A2 (en) 2011-06-10 2012-12-13 Biovest International, Inc. Method and apparatus for antibody production and purification
JP6095671B2 (ja) * 2011-10-07 2017-03-15 ポール テクノロジー ユーケイ リミテッドPall Technology Uk Limited 流体処理制御システムおよび対応する方法
US9683207B2 (en) 2012-05-01 2017-06-20 FiberCell Production Solutions LLC Method for growing cells in hollow fibers
CN104583386A (zh) * 2012-08-20 2015-04-29 泰尔茂比司特公司 浓缩穿过细胞生长腔室循环的流体的组分
EP2909302A4 (en) * 2012-08-28 2016-07-27 Biovest Int Inc BIOFABRICATION FILAMENT AND METHOD FOR LARGE-SCALE PRODUCTION OF CELLS, VIRUSES AND BIOMOLECULES
WO2014036488A1 (en) 2012-08-31 2014-03-06 Biovest International, Inc. Methods for producing high-fidelity autologous idiotype vaccines
EP2706112A1 (en) * 2012-09-06 2014-03-12 Université Catholique De Louvain Fluidic device for studying of surface-dwelling multicellular layers and microbial biofilms
RU2615179C2 (ru) 2012-09-06 2017-04-04 Плуристем Лтд. Устройства и способы культивирования клеток
ES2489315B1 (es) * 2013-01-16 2015-09-09 Fundación Para La Investigación Biomédica Del Hospital Universitario La Paz Cámara de cultivo celular sobre biomateriales
HUE042094T2 (hu) 2013-02-22 2019-06-28 Genzyme Corp Mikrokarrier-perfúziós tenyésztési eljárások és alkalmazásuk
WO2015027186A1 (en) 2013-08-22 2015-02-26 University Of Pittsburgh-Of The Commonwealth System Of Higher Education A modular, microfluidic, mechanically active bioreactor for 3d, multi-tissue, tissue culture
WO2015048712A2 (en) * 2013-09-30 2015-04-02 Weyerhaeuser Nr Company Independent ports in automated bioreactor system
WO2015073913A1 (en) 2013-11-16 2015-05-21 Terumo Bct, Inc. Expanding cells in a bioreactor
EP3105311A1 (en) 2014-02-10 2016-12-21 Univercells NV System, apparatus and method for biomolecules production
EP3110428A4 (en) * 2014-02-28 2017-11-22 Bdbc Sciences Corp. A system for tissue manipulation
US10039244B2 (en) * 2014-03-04 2018-08-07 Greenonyx Ltd Systems and methods for cultivating and distributing aquatic organisms
WO2015148704A1 (en) 2014-03-25 2015-10-01 Terumo Bct, Inc. Passive replacement of media
CN106232801A (zh) 2014-04-28 2016-12-14 维瓦生物细胞股份公司 自动化细胞培养和收获装置
TWI743024B (zh) * 2014-06-06 2021-10-21 美商健臻公司 灌注培養方法及其用途
EP3198006B1 (en) 2014-09-26 2021-03-24 Terumo BCT, Inc. Scheduled feed
JP6807749B2 (ja) * 2014-12-11 2021-01-06 株式会社アイカムス・ラボ 細胞培養方法及び細胞培養装置
WO2017004592A1 (en) 2015-07-02 2017-01-05 Terumo Bct, Inc. Cell growth with mechanical stimuli
EP3359642A4 (en) 2015-10-06 2019-05-08 University of Pittsburgh- Of the Commonwealth System of Higher Education MICROFLUIDIC TISSUE DEVELOPMENT SYSTEMS
CA3002810A1 (en) 2015-10-20 2017-04-27 Sharmistha MAZUMDER Apparatus, systems and methods for growing microorganisms
WO2017087535A1 (en) * 2015-11-17 2017-05-26 Oregon State University Bio-lamina bioreactors and methods of making and using the same
KR20240173213A (ko) * 2016-01-26 2024-12-10 베링거 인겔하임 인터내셔날 게엠베하 연결된 관류 대 연속-유동식 교반-탱크 반응기 세포 배양 시스템
US11965175B2 (en) 2016-05-25 2024-04-23 Terumo Bct, Inc. Cell expansion
US11685883B2 (en) 2016-06-07 2023-06-27 Terumo Bct, Inc. Methods and systems for coating a cell growth surface
US11104874B2 (en) 2016-06-07 2021-08-31 Terumo Bct, Inc. Coating a bioreactor
GB2554635A (en) * 2016-08-03 2018-04-11 Northwick Park Institute For Medical Res Ltd Bioreactors and methods for processing biological material
JP6942448B2 (ja) * 2016-08-08 2021-09-29 株式会社カネカ 細胞培養容器、これを用いた細胞培養システム、および細胞培養方法
US11624046B2 (en) 2017-03-31 2023-04-11 Terumo Bct, Inc. Cell expansion
CN117247899A (zh) 2017-03-31 2023-12-19 泰尔茂比司特公司 细胞扩增
US12234441B2 (en) 2017-03-31 2025-02-25 Terumo Bct, Inc. Cell expansion
TWI675696B (zh) * 2017-06-01 2019-11-01 美商Emd密理博公司 用於灌注應用之切向流過濾裝置
KR102176500B1 (ko) 2017-07-21 2020-11-09 포항공과대학교 산학협력단 관류식 바이오 리액터를 사용한 세포밖 소포체 분비량 향상 방법 및 이를 위한 시스템
US11680241B2 (en) 2018-02-08 2023-06-20 University Of Florida Research Foundation, Inc. Perfusion enabled bioreactors
WO2020013221A1 (ja) * 2018-07-12 2020-01-16 日本曹達株式会社 培養装置、担体、および培養対象回収方法
JP7133409B2 (ja) * 2018-09-18 2022-09-08 テルモ株式会社 バイオリアクタ、細胞培養システム及び細胞培養方法
JP7133410B2 (ja) * 2018-09-18 2022-09-08 テルモ株式会社 バイオリアクタ、細胞培養システム及び細胞培養方法
IL263127B (en) * 2018-11-19 2022-07-01 The Interdisciplinary Center Herzliya Cc A liquid-based biological system
KR20210108406A (ko) * 2018-12-21 2021-09-02 론자 워커스빌 아이엔씨. 바이러스 벡터의 자동화된 생산
WO2020163329A1 (en) 2019-02-05 2020-08-13 Corning Incorporated Woven cell culture substrates
IL288338B2 (en) 2019-05-28 2023-02-01 Upside Foods Inc Apparatuses and methods for preparing a comestible meat product
WO2021038997A1 (ja) * 2019-08-29 2021-03-04 ファナック株式会社 細胞製造装置
US11118151B2 (en) 2019-11-05 2021-09-14 Corning Incorporated Fixed bed bioreactor and methods of using the same
AU2020386085B2 (en) 2019-11-20 2022-08-11 Upside Foods, Inc. Apparatuses and systems for preparing a meat product
WO2021108089A1 (en) * 2019-11-27 2021-06-03 Corning Incorporated Radial flow fixed bed bioreactor and methods of using the same
KR102417445B1 (ko) * 2019-11-29 2022-07-06 주식회사 아모그린텍 세포배양장치
USD961115S1 (en) * 2020-01-20 2022-08-16 Adva Biotechnology Ltd. Bioreactor cabinet
KR20220166825A (ko) * 2020-04-10 2022-12-19 사우스웨스트 리서치 인스티튜트 바이러스 벡터 생산을 위한 3차원 생물 반응기
CN116194569A (zh) * 2020-08-17 2023-05-30 东北大学 用于金属纳米颗粒生产的微生物辅助灌流系统
EP4314244B1 (en) 2021-03-23 2025-07-23 Terumo BCT, Inc. Cell capture and expansion
US11752509B2 (en) 2021-06-17 2023-09-12 Upside Foods, Inc. Fluid dispenser for recovering material from a surface
US20230066039A1 (en) * 2021-09-01 2023-03-02 Global Life Sciences Solutions Usa Llc Perfusion filter membrane and method of use
US12606788B2 (en) 2021-09-01 2026-04-21 Global Life Sciences Solutions Usa Llc Perfusion filter membrane assembly
US12241050B2 (en) 2021-09-01 2025-03-04 Global Life Sciences Solutions Usa, Llc Perfusion filter assembly for increased cross flow
WO2023121655A1 (en) * 2021-12-21 2023-06-29 Bayer Aktiengesellschaft Improved cell culture method
US12209689B2 (en) 2022-02-28 2025-01-28 Terumo Kabushiki Kaisha Multiple-tube pinch valve assembly
WO2023164273A1 (en) * 2022-02-28 2023-08-31 University Of Georgia Research Foundation, Inc. Bioreactor for cell expansion and cultivation
USD1099116S1 (en) 2022-09-01 2025-10-21 Terumo Bct, Inc. Display screen or portion thereof with a graphical user interface for displaying cell culture process steps and measurements of an associated bioreactor device
WO2024073071A1 (en) * 2022-09-29 2024-04-04 The University Of Akron Chamber assembly for ex-vivo electroretinogram with filleted perfusion inlet and outlet
US11981884B2 (en) 2022-10-17 2024-05-14 Upside Foods, Inc. Pipe-based bioreactors for producing comestible meat products and methods of using the same
WO2026046948A1 (en) * 2024-08-26 2026-03-05 Qorium B.V. Perfusion bioreactor, scaffold cassette, and method for culturing cells

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4804628A (en) * 1984-10-09 1989-02-14 Endotronics, Inc. Hollow fiber cell culture device and method of operation
US5266476A (en) * 1985-06-18 1993-11-30 Yeda Research & Development Co., Ltd. Fibrous matrix for in vitro cell cultivation
US5786215A (en) * 1987-05-20 1998-07-28 Baxter International Inc. Method for culturing animal cells
DE3923279A1 (de) * 1989-07-14 1990-01-18 Will W Prof Dr Minuth Minusheets ist ein neues produkt, um zellen in beliebigen behaeltnissen in hochdifferenzierter form auf einer moeglichst natuerlichen unterlage zu kultivieren
US5126269A (en) * 1990-09-13 1992-06-30 Life Techologies, Inc. Spin filter perfusion bioreactor (sfpb) cell culture apparatus
BE1009306A5 (fr) * 1995-04-28 1997-02-04 Baxter Int Bioreacteur.
CA2279996A1 (en) * 1997-01-16 1998-07-16 Yissum Research Development Company Of The Hebrew University In Jerusale M A device and method for performing a biological modification of a fluid
WO1998050522A1 (en) * 1997-05-07 1998-11-12 Baxter International Inc. Method and apparatus for high volume production of viral particles
US6875605B1 (en) * 2002-08-21 2005-04-05 Florida State University Research Foundation, Inc. Modular cell culture bioreactor and associated methods
US6943008B1 (en) * 2002-08-21 2005-09-13 Florida State University Research Foundation, Inc. Bioreactor for cell culture
US7534601B2 (en) * 2002-08-27 2009-05-19 Vanderbilt University Capillary perfused bioreactors with multiple chambers
CN101120083B (zh) * 2003-10-08 2013-03-27 威尔森沃尔夫制造公司 利用透气性材料进行细胞培养的方法及装置
US7767446B2 (en) * 2004-09-16 2010-08-03 Becton, Dickinson And Company Perfusion bioreactors for culturing cells
JP4639799B2 (ja) * 2004-12-27 2011-02-23 カシオ計算機株式会社 カメラ装置、レンズ駆動方法
WO2007071072A1 (en) * 2005-12-22 2007-06-28 Corporation De L'ecole Polytechnique De Montreal High-rate perfusion bioreactor
EP2029722B1 (en) * 2006-05-22 2019-10-16 Biovest International, Inc. Method and system for the production of cells

Also Published As

Publication number Publication date
CA2741481A1 (en) 2010-04-29
EP2346984A4 (en) 2013-10-30
AU2009308354A1 (en) 2010-04-29
WO2010048417A2 (en) 2010-04-29
US20110212493A1 (en) 2011-09-01
EP2346984A2 (en) 2011-07-27
WO2010048417A3 (en) 2010-08-19
JP2012506257A (ja) 2012-03-15

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