IL164182A0 - Zymogenic nucleic acid detection methods, and related molecules and kits - Google Patents

Zymogenic nucleic acid detection methods, and related molecules and kits

Info

Publication number
IL164182A0
IL164182A0 IL16418299A IL16418299A IL164182A0 IL 164182 A0 IL164182 A0 IL 164182A0 IL 16418299 A IL16418299 A IL 16418299A IL 16418299 A IL16418299 A IL 16418299A IL 164182 A0 IL164182 A0 IL 164182A0
Authority
IL
Israel
Prior art keywords
nucleic acid
kits
zymogenic
target
detection methods
Prior art date
Application number
IL16418299A
Other languages
English (en)
Original Assignee
Johnson & Johnson Res Pty Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Johnson & Johnson Res Pty Ltd filed Critical Johnson & Johnson Res Pty Ltd
Publication of IL164182A0 publication Critical patent/IL164182A0/xx

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6823—Release of bound markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6867—Replicase-based amplification, e.g. using Q-beta replicase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
IL16418299A 1998-03-05 1999-02-22 Zymogenic nucleic acid detection methods, and related molecules and kits IL164182A0 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US7689998P 1998-03-05 1998-03-05
PCT/IB1999/000754 WO1999045146A1 (fr) 1998-03-05 1999-02-22 Methodes de detection d'acide nucleique utilisant des zymogenes, molecules et trousses associees

Publications (1)

Publication Number Publication Date
IL164182A0 true IL164182A0 (en) 2005-12-18

Family

ID=22134859

Family Applications (2)

Application Number Title Priority Date Filing Date
IL13812699A IL138126A0 (en) 1998-03-05 1999-02-22 Zymogenic nucleic acid detection methods and related molecules and kits
IL16418299A IL164182A0 (en) 1998-03-05 1999-02-22 Zymogenic nucleic acid detection methods, and related molecules and kits

Family Applications Before (1)

Application Number Title Priority Date Filing Date
IL13812699A IL138126A0 (en) 1998-03-05 1999-02-22 Zymogenic nucleic acid detection methods and related molecules and kits

Country Status (13)

Country Link
US (3) US6140055A (fr)
EP (1) EP1062367B1 (fr)
JP (1) JP4318856B2 (fr)
KR (1) KR100748863B1 (fr)
CN (1) CN1167811C (fr)
AT (1) ATE414169T1 (fr)
AU (1) AU768627B2 (fr)
BR (1) BR9908567A (fr)
CA (1) CA2321948A1 (fr)
DE (1) DE69939896D1 (fr)
IL (2) IL138126A0 (fr)
WO (1) WO1999045146A1 (fr)
ZA (1) ZA991772B (fr)

Families Citing this family (40)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6261808B1 (en) * 1992-08-04 2001-07-17 Replicon, Inc. Amplification of nucleic acid molecules via circular replicons
WO1994003624A1 (fr) * 1992-08-04 1994-02-17 Auerbach Jeffrey I Procedes d'amplification isothermique de molecules d'acide nucleique
US6140055A (en) 1998-03-05 2000-10-31 Johnson & Johnson Research Pty Limited Zymogenic nucleic acid detection methods and related kits
DE19811618C1 (de) * 1998-03-17 2000-08-31 Andreas Jenne Ribozym codierende DNA und ein Oligonucleotidsubstrat enthaltende Zusammensetzung und Verfahren zur Messung von Transkriptionsraten
AUPQ495700A0 (en) * 2000-01-05 2000-02-03 Johnson & Johnson Research Pty. Limited Method for concurrent amplification and real time detection of polymorphic nucleic acid sequences
US7205129B1 (en) * 2000-02-28 2007-04-17 Qiagen Gmbh Method for reducing artifacts in nucleic acid amplification
JP2004515219A (ja) * 2000-06-15 2004-05-27 ボード・オブ・リージェンツ，ザ・ユニヴァーシティ・オヴ・テキサス・システム 調節可能な触媒活性な核酸
US7125660B2 (en) 2000-09-13 2006-10-24 Archemix Corp. Nucleic acid sensor molecules and methods of using same
WO2003014375A2 (fr) * 2001-08-09 2003-02-20 Archemix Corporation Molecules de sonde d'acide nucleique et methodes d'utilisation de ces dernieres
US7297485B2 (en) * 2001-10-15 2007-11-20 Qiagen Gmbh Method for nucleic acid amplification that results in low amplification bias
US6617137B2 (en) * 2001-10-15 2003-09-09 Molecular Staging Inc. Method of amplifying whole genomes without subjecting the genome to denaturing conditions
US7955795B2 (en) * 2003-06-06 2011-06-07 Qiagen Gmbh Method of whole genome amplification with reduced artifact production
US9487823B2 (en) 2002-12-20 2016-11-08 Qiagen Gmbh Nucleic acid amplification
MXPA05010429A (es) 2003-03-31 2005-11-04 Hoffmann La Roche Composiciones y metodos para detectar ciertos flavivirus que incluyen miembros del serogrupo del virus de la encefalitis japonesa.
US8043834B2 (en) 2003-03-31 2011-10-25 Qiagen Gmbh Universal reagents for rolling circle amplification and methods of use
US7667024B2 (en) * 2003-11-19 2010-02-23 Allelogic Biosciences Corp. Oligonucleotides labeled with a plurality of fluorophores
WO2005085474A2 (fr) * 2004-03-02 2005-09-15 Clontech Laboratories Inc. Substrats d'acides nucleiques etiquetes a transfert d'energie fluorescente et procedes d'utilisation de ceux-ci
US7939251B2 (en) 2004-05-06 2011-05-10 Roche Molecular Systems, Inc. SENP1 as a marker for cancer
US20060211018A1 (en) * 2005-02-08 2006-09-21 Applera Corporation Nucleozymes and methods of use
EP1863908B1 (fr) 2005-04-01 2010-11-17 Qiagen GmbH Transcription inverse et amplification d'arn avec degradation simultanee d'adn
EP1880021A2 (fr) * 2005-05-02 2008-01-23 Stratagene California Compositions de sondes/amorces oligonucleotidiques et procedes de detection de polynucleotides
EP1762627A1 (fr) 2005-09-09 2007-03-14 Qiagen GmbH Procédé pour l'activation d'acides nucléiques pour effectuer une réaction d'une polymérase
DK1948822T3 (da) 2005-10-07 2011-10-17 Johnson & Johnson Res Pty Ltd Multikomponent-nukleinsyreenzymer og fremgangsmåder til deres anvendelse
WO2007056312A2 (fr) * 2005-11-07 2007-05-18 Clontech Laboratories, Inc. Marqueur rapporteur universel pour essais bases sur un nucleozyme
CN101365808B (zh) * 2005-12-07 2012-04-25 泰博特克药品有限公司 用于评估hiv病毒适应度的方法、质粒载体和引物
WO2008001376A2 (fr) * 2006-06-28 2008-01-03 Yissum Research Development Company Of The Hebrew University Of Jerusalem Détection d'analytes dans un milieu
DE102006035600B3 (de) * 2006-07-27 2007-11-08 Epigenomics Ag Verfahren zum Nachweis eines Methylierungsmusters
EP1882746A3 (fr) * 2006-07-27 2008-03-19 Epigenomics AG Procédé permettand de determiner le modèle de méthylation
JP5688507B2 (ja) 2006-10-06 2015-03-25 スピーデクス ピーティーワイ リミテッド 分子スイッチおよびそれらの使用のための方法
US9938641B2 (en) * 2006-12-18 2018-04-10 Fluidigm Corporation Selection of aptamers based on geometry
ES2425066T3 (es) 2007-04-05 2013-10-11 Speedx Pty Ltd Enzimas y complejos de ácidos nucleicos y métodos para su uso
EP2071035A1 (fr) 2007-12-13 2009-06-17 Epigenomics AG Facilitateur et procédé d'amplification
WO2010147848A2 (fr) 2009-06-15 2010-12-23 Rd Biosciences, Inc. Coffrets et procédés pour une amplification et une détection sélective de cibles d'acide nucléique
CN101907573B (zh) * 2010-08-17 2012-08-22 复旦大学 一种高灵敏度的集成cmos光学生物芯片
WO2012034130A2 (fr) 2010-09-10 2012-03-15 Qiagen Procédés et compositions pour la détection d'acides nucléiques
RU2690374C2 (ru) 2011-04-15 2019-06-03 Бектон, Дикинсон Энд Компани Сканирующий в режиме реального времени микрожидкостный термоциклер и способы синхронизированных термоциклирования и сканирующего оптического обнаружения
CN104040238B (zh) 2011-11-04 2017-06-27 汉迪拉布公司 多核苷酸样品制备装置
AU2013202354A1 (en) * 2012-06-18 2014-01-16 Speedx Pty Ltd Target detection and signal amplification
CN103013953B (zh) * 2012-10-19 2014-09-17 中国科学院成都生物研究所 一种剪切dna的dna酶
CN105518156A (zh) * 2013-09-05 2016-04-20 日本电气方案创新株式会社 用于制备样品的方法和用于分析靶标的方法

Family Cites Families (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4000159A (en) * 1974-06-28 1976-12-28 Phillips Petroleum Company Preparation of n,n-disubstituted thioamides
US4965188A (en) * 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US5176995A (en) * 1985-03-28 1993-01-05 Hoffmann-La Roche Inc. Detection of viruses by amplification and hybridization
US4683195A (en) * 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4800159A (en) 1986-02-07 1989-01-24 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences
JP3276955B2 (ja) * 1988-09-30 2002-04-22 ジーン−トラック・システムス Ｒｎａの鋳型の末端に結合したプローブ構造およびその使用方法
US5112734A (en) * 1989-05-26 1992-05-12 Gene-Trak Systems Target-dependent synthesis of an artificial gene for the synthesis of a replicatable rna
NZ314629A (en) * 1991-01-17 2000-08-25 Gen Hospital Corp Use trans-splicing ribozymes to prepare medicaments for gene therapies
GB9115909D0 (en) * 1991-07-23 1991-09-04 Nickerson Int Seed Recombinant dna
EP1195442A3 (fr) * 1992-12-04 2002-07-31 Innovir Laboratories, Inc. Amplification des signaux par ribosyme dans une méthode diagnostique
US5516664A (en) * 1992-12-23 1996-05-14 Hyman; Edward D. Enzymatic synthesis of repeat regions of oligonucleotides
US5436143A (en) * 1992-12-23 1995-07-25 Hyman; Edward D. Method for enzymatic synthesis of oligonucleotides
KR100218726B1 (ko) * 1992-12-30 1999-09-01 김영환 고집적 반도체 소자의 접속장치 및 그 제조방법
IL105894A (en) * 1993-06-03 1997-02-18 Asher Nathan Method for the detection of nucleic acid sequence by using a detection ensemble and an amplification ensemble
US5582988A (en) * 1994-09-15 1996-12-10 Johnson & Johnson Clinical Diagnostics, Inc. Methods for capture and selective release of nucleic acids using weakly basic polymer and amplification of same
IL111857A (en) * 1994-12-02 1999-09-22 Intelligene Ltd Detection of nucleic acid sequences
US5807718A (en) * 1994-12-02 1998-09-15 The Scripps Research Institute Enzymatic DNA molecules
JPH11500917A (ja) * 1995-02-27 1999-01-26 インテリジェン・リミテッド 生体分子の検出
AUPN245295A0 (en) * 1995-04-13 1995-05-11 Johnson & Johnson Research Pty. Limited Assay for genetic abnormalities
EP0833945B1 (fr) * 1995-06-07 2002-01-30 Merck & Co., Inc. Procede de detection par extension catalysee par l'adn polymerase
BRPI9809433B1 (pt) * 1997-04-29 2015-09-08 Scripps Research Inst moléculas de dna enzimáticas
DE19805593A1 (de) * 1998-02-12 1999-08-19 Voith Sulzer Papiertech Patent Eindicker
US6140055A (en) 1998-03-05 2000-10-31 Johnson & Johnson Research Pty Limited Zymogenic nucleic acid detection methods and related kits

Also Published As

Publication number Publication date
AU3340999A (en) 1999-09-20
WO1999045146A1 (fr) 1999-09-10
EP1062367B1 (fr) 2008-11-12
JP4318856B2 (ja) 2009-08-26
KR20010041591A (ko) 2001-05-25
ZA991772B (en) 2000-10-12
AU768627B2 (en) 2003-12-18
EP1062367A1 (fr) 2000-12-27
CN1167811C (zh) 2004-09-22
US6365724B2 (en) 2002-04-02
BR9908567A (pt) 2000-12-12
US6201113B1 (en) 2001-03-13
US20010001063A1 (en) 2001-05-10
CN1319142A (zh) 2001-10-24
IL138126A0 (en) 2001-10-31
DE69939896D1 (de) 2008-12-24
EP1062367A4 (fr) 2003-07-30
JP2002505117A (ja) 2002-02-19
CA2321948A1 (fr) 1999-09-10
KR100748863B1 (ko) 2007-08-13
US6140055A (en) 2000-10-31
ATE414169T1 (de) 2008-11-15

Similar Documents

Publication Publication Date Title
IL164182A0 (en) Zymogenic nucleic acid detection methods, and related molecules and kits
FI950487A (fi) Menetelmä, reagenssi ja testipakkaus nukleiinihapposekvenssien totamiseksi ja monistamiseksi
CO4870792A1 (es) Compuestos y conjunto de medios de diagnostico para detectar y vigilar la progresion de cancer de prostata
AU4969996A (en) Methods for the isothermal amplification of nucleic acid molecules
WO2003060163A3 (fr) Discrimination et detection de sequences nucleotidiques cibles utilisant la spectrometrie de masse
ES2173670T3 (es) Procedimiento de identificacion de acidos nucleicos mediante espectrometria de masas laser de desorcion/ionizacion asistidas por matriz.
DE50312579D1 (de) Vorrichtung zur Detektion von Targetmolekülen
WO2005010494A3 (fr) Detection d'analytes multiplexee
AU2001282920A1 (en) Methods for detecting and assaying nucleic acid sequences
WO2003062462A3 (fr) Methode permettant d'isoler des acides nucleiques et des proteines contenus dans un echantillon unique
WO2001027624A3 (fr) Compositions et procedes permettant de detecter une modification de proteine et une activite enzymatique
CY1106724T1 (el) Εμβαπτιζομενος χαρτης αποξηραμενων αντιδραστηριων και μεθοδος για την ανιχνευση ή και προσδιορισμο ειδικων αλληλουχιων νουκλεϊκων οξεων
EP1288313A3 (fr) Système et méthode pour analyser des molécules d'acide nucléique
WO2000031305A3 (fr) Methodes d'extension d'amorces permettant de detecter des acides nucleiques au moyen de molecules donneuses et receveuses
SG153624A1 (en) Isometric primer extension method and kit for detection and quantification of specific nucleic acid
EP0843019A3 (fr) Méthode pour la détermination d'une séquence d'acide nucléique spécifique, et un réactif correspondant
ATE449868T1 (de) Methoden, testsätze und kompositionen zur detektion und quantifizierung von zielsequenzen
DK1546354T3 (da) Analytdetektion
AU781702C (en) Method and nucleic acids for determining the presence of micro-organisms specific to the brewing process
ATE476526T1 (de) Verfahren zur geno- und pathotypisierung von pseudomonas aeruginosa
WO2000055363A3 (fr) Analyse de l'expression genique differentielle
SE9500341D0 (sv) Method for detecting DNA sequence variations
DE60121547D1 (de) Electrochemisches verfahren zum nachweis von nukleinsäuren
ATE455864T1 (de) Verfahren zum nachweis von in einer polymerase- kettenreaktion amplifizierten nukleinsäuremolekülen
WO1999058971A3 (fr) Procedes servant a quantifier de bas niveaux de modifications de sequences de nucleotides