IL125915A - A method for classifying pixels into groups according to their spectrum using a number of wide-range filters and a device for this purpose - Google Patents
A method for classifying pixels into groups according to their spectrum using a number of wide-range filters and a device for this purposeInfo
- Publication number
- IL125915A IL125915A IL12591598A IL12591598A IL125915A IL 125915 A IL125915 A IL 125915A IL 12591598 A IL12591598 A IL 12591598A IL 12591598 A IL12591598 A IL 12591598A IL 125915 A IL125915 A IL 125915A
- Authority
- IL
- Israel
- Prior art keywords
- filters
- fluorophores
- wide
- emission
- pixels
- Prior art date
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- General Physics & Mathematics (AREA)
- Optics & Photonics (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Pathology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US08/917,213 US5834203A (en) | 1997-08-25 | 1997-08-25 | Method for classification of pixels into groups according to their spectra using a plurality of wide band filters and hardwire therefore |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| IL125915A0 IL125915A0 (en) | 1999-04-11 |
| IL125915A true IL125915A (en) | 2001-11-25 |
Family
ID=25438492
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| IL12591598A IL125915A (en) | 1997-08-25 | 1998-08-25 | A method for classifying pixels into groups according to their spectrum using a number of wide-range filters and a device for this purpose |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US5834203A (fr) |
| EP (2) | EP0899558B1 (fr) |
| JP (1) | JP3130508B2 (fr) |
| DE (2) | DE69809161T2 (fr) |
| ES (1) | ES2186097T3 (fr) |
| IL (1) | IL125915A (fr) |
Families Citing this family (55)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5936731A (en) * | 1991-02-22 | 1999-08-10 | Applied Spectral Imaging Ltd. | Method for simultaneous detection of multiple fluorophores for in situ hybridization and chromosome painting |
| US6018587A (en) * | 1991-02-21 | 2000-01-25 | Applied Spectral Imaging Ltd. | Method for remote sensing analysis be decorrelation statistical analysis and hardware therefor |
| US6690817B1 (en) * | 1993-08-18 | 2004-02-10 | Applied Spectral Imaging Ltd. | Spectral bio-imaging data for cell classification using internal reference |
| US6165734A (en) * | 1995-12-12 | 2000-12-26 | Applied Spectral Imaging Ltd. | In-situ method of analyzing cells |
| US7498164B2 (en) | 1998-05-16 | 2009-03-03 | Applied Biosystems, Llc | Instrument for monitoring nucleic acid sequence amplification reaction |
| CN1664562A (zh) | 1998-05-16 | 2005-09-07 | 阿普尔拉公司 | 用于监测dna聚合酶链反应的仪器 |
| US6818437B1 (en) * | 1998-05-16 | 2004-11-16 | Applera Corporation | Instrument for monitoring polymerase chain reaction of DNA |
| US6642062B2 (en) | 1998-09-03 | 2003-11-04 | Trellis Bioinformatics, Inc. | Multihued labels |
| US6453060B1 (en) * | 1999-06-29 | 2002-09-17 | Tri Path Imaging, Inc. | Method and apparatus for deriving separate images from multiple chromogens in a branched image analysis system |
| EP1269162A4 (fr) * | 2000-02-25 | 2005-01-19 | Cambridge Res & Instrmnt Inc | Systeme et procede de dosage par polarisation de la fluorescence de plusieurs marqueurs |
| US6627748B1 (en) | 2000-09-11 | 2003-09-30 | The Trustees Of Columbia University In The City Of New York | Combinatorial fluorescence energy transfer tags and their applications for multiplex genetic analyses |
| US20060057565A1 (en) * | 2000-09-11 | 2006-03-16 | Jingyue Ju | Combinatorial fluorescence energy transfer tags and uses thereof |
| WO2002022883A1 (fr) * | 2000-09-11 | 2002-03-21 | The Trustees Of Columbia University In The City Of New York | Indicateurs de transfert d'energie de fluorescence combinatoire et utilisations associees |
| ATE356222T1 (de) | 2000-10-06 | 2007-03-15 | Univ Columbia | Massives parallelverfahren zur dekodierung von dna und rna |
| US9708358B2 (en) | 2000-10-06 | 2017-07-18 | The Trustees Of Columbia University In The City Of New York | Massive parallel method for decoding DNA and RNA |
| US20100279341A1 (en) * | 2000-10-24 | 2010-11-04 | Tissuegnostics Gmbh | Methods and system for analyzing cells |
| AT411066B (de) * | 2000-10-24 | 2003-09-25 | Steiner Georg E | Verfahren und anordnung zur untersuchung von zellen |
| DE10151217B4 (de) * | 2001-10-16 | 2012-05-16 | Carl Zeiss Microlmaging Gmbh | Verfahren zum Betrieb eines Laser-Scanning-Mikroskops |
| DE10206979A1 (de) * | 2002-02-20 | 2003-08-21 | Leica Microsystems | Verfahren zum Benutzertraining für ein Scanmikroskop, Scanmikroskop und Software zum Benutzertraining für ein Scanmikroskop |
| US7074597B2 (en) * | 2002-07-12 | 2006-07-11 | The Trustees Of Columbia University In The City Of New York | Multiplex genotyping using solid phase capturable dideoxynucleotides and mass spectrometry |
| AU2003273668A1 (en) * | 2002-10-02 | 2004-04-23 | Ifire Technology Corp.Lumen Health Innovations, Inc. | Apparatus and methods relating to high speed spectroscopy and excitation-emission matrices |
| US20050032081A1 (en) * | 2002-12-13 | 2005-02-10 | Jingyue Ju | Biomolecular coupling methods using 1,3-dipolar cycloaddition chemistry |
| US7321791B2 (en) * | 2003-09-23 | 2008-01-22 | Cambridge Research And Instrumentation, Inc. | Spectral imaging of deep tissue |
| US8634607B2 (en) | 2003-09-23 | 2014-01-21 | Cambridge Research & Instrumentation, Inc. | Spectral imaging of biological samples |
| US7315371B2 (en) * | 2004-01-23 | 2008-01-01 | P&P Optica Inc. | Multi-channel spectrum analyzer |
| US7622279B2 (en) | 2004-03-03 | 2009-11-24 | The Trustees Of Columbia University In The City Of New York | Photocleavable fluorescent nucleotides for DNA sequencing on chip constructed by site-specific coupling chemistry |
| EP1846869B1 (fr) | 2005-01-27 | 2011-10-05 | Cambridge Research & Instrumentation, Inc. | Classification de caracteristiques d'images |
| DE102005022880B4 (de) * | 2005-05-18 | 2010-12-30 | Olympus Soft Imaging Solutions Gmbh | Trennung spektral oder farblich überlagerter Bildbeiträge in einem Mehrfarbbild, insbesondere in transmissionsmikroskopischen Mehrfarbbildern |
| WO2007002204A2 (fr) | 2005-06-21 | 2007-01-04 | The Trustees Of Columbia University In The City Of New York | Procedes de pyrosequencage et compositions associees |
| JP2007010340A (ja) | 2005-06-28 | 2007-01-18 | Olympus Corp | カメラ装置 |
| DE102006052542B4 (de) * | 2006-11-06 | 2008-08-14 | Hochschule für angewandte Wissenschaft und Kunst (HAWK) Hildesheim | Bilderfassungseinrichtung |
| US7883869B2 (en) | 2006-12-01 | 2011-02-08 | The Trustees Of Columbia University In The City Of New York | Four-color DNA sequencing by synthesis using cleavable fluorescent nucleotide reversible terminators |
| DE102007014413B4 (de) * | 2007-03-17 | 2016-02-04 | DüRR DENTAL AG | Verfahren zum Auswerten von Fluoreszenzbildsätzen und Vorrichtung zu seiner Durchführung |
| US8330087B2 (en) * | 2007-10-16 | 2012-12-11 | Cambridge Research & Instrumentation, Inc. | Spectral imaging system with dynamic optical correction |
| US20110014611A1 (en) | 2007-10-19 | 2011-01-20 | Jingyue Ju | Design and synthesis of cleavable fluorescent nucleotides as reversible terminators for dna sequences by synthesis |
| US9115163B2 (en) | 2007-10-19 | 2015-08-25 | The Trustees Of Columbia University In The City Of New York | DNA sequence with non-fluorescent nucleotide reversible terminators and cleavable label modified nucleotide terminators |
| DE102008034008B4 (de) | 2008-07-21 | 2010-07-01 | Carl Zeiss Surgical Gmbh | Filtersatz zur Beobachtung von Fluoreszenzstrahlung in biologischem Gewebe |
| US7969568B2 (en) * | 2008-10-24 | 2011-06-28 | Applied Materials, Inc. | Spectrographic metrology of patterned wafers |
| US8774488B2 (en) | 2010-03-11 | 2014-07-08 | Cellscape Corporation | Method and device for identification of nucleated red blood cells from a maternal blood sample |
| JP5494351B2 (ja) * | 2010-08-24 | 2014-05-14 | ソニー株式会社 | 蛍光強度補正方法、蛍光強度算出方法及び蛍光強度算出装置並びに蛍光強度補正プログラム |
| US9624539B2 (en) | 2011-05-23 | 2017-04-18 | The Trustees Of Columbia University In The City Of New York | DNA sequencing by synthesis using Raman and infrared spectroscopy detection |
| US8682047B2 (en) * | 2011-12-05 | 2014-03-25 | Illinois Tool Works Inc. | Method and apparatus for machine vision counting and annotation |
| CN104271767B (zh) | 2012-02-03 | 2017-08-25 | 加州理工学院 | 多路生化测定中信号的编码和解码 |
| US9791372B2 (en) * | 2012-08-03 | 2017-10-17 | California Institute Of Technology | Multiplexing and quantification in PCR with reduced hardware and requirements |
| WO2014144883A1 (fr) | 2013-03-15 | 2014-09-18 | The Trustees Of Columbia University In The City Of New York | Molecules marquees par des amas de raman destinees a l'imagerie biologique |
| EP3329209B1 (fr) * | 2015-07-30 | 2022-11-09 | Technology Innovation Momentum Fund (Israel) Limited Partnership | Procédé et système d'imagerie spectrale |
| EP3251578A1 (fr) * | 2016-05-30 | 2017-12-06 | Leica Instruments (Singapore) Pte. Ltd. | Dispositif médical pour l'observation d'un objet partiellement fluorescent, à l'aide d'un système de filtre avec une fenêtre de transmission |
| US10724958B2 (en) * | 2017-11-06 | 2020-07-28 | Senors Unlimited, Inc. | Imaging devices, imaging arrangements, and imaging methods |
| JP7098146B2 (ja) * | 2018-07-05 | 2022-07-11 | 株式会社Iddk | 顕微観察装置、蛍光検出器及び顕微観察方法 |
| DE102019101773B9 (de) * | 2019-01-24 | 2021-11-25 | Carl Zeiss Meditec Ag | Mikroskopiesystem und Verfahren zum Betreiben eines Mikroskopiesystems |
| US11143587B2 (en) * | 2019-04-02 | 2021-10-12 | Becton, Dickinson And Company | Compensation editor |
| WO2021127019A1 (fr) * | 2019-12-17 | 2021-06-24 | Applied Materials, Inc. | Système et procédé d'acquisition et de traitement d'images d'hybridation in-situ de fluorescence multiplexées |
| JP7543824B2 (ja) * | 2020-10-09 | 2024-09-03 | セイコーエプソン株式会社 | 画像解析装置、画像解析方法、及び画像解析プログラム |
| WO2022149744A1 (fr) * | 2021-01-05 | 2022-07-14 | 한국과학기술원 | Procédé et dispositif de séparation de couleurs multiples par minimisation de quantité d'informations mutuelles répétitives concernant une structure de mise à jour de canal simultanée |
| EP4341670A1 (fr) * | 2021-05-19 | 2024-03-27 | Leica Microsystems CMS GmbH | Procédé et dispositif d'analyse d'un échantillon biologique |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1994001968A1 (fr) * | 1992-07-08 | 1994-01-20 | Oncor, Inc. | Systeme de prise d'images couleur a faible niveau de lumiere, utilisant un appareil photographique integrateur a refroidissement |
| US5556764A (en) * | 1993-02-17 | 1996-09-17 | Biometric Imaging, Inc. | Method and apparatus for cell counting and cell classification |
| US5863504A (en) * | 1995-03-16 | 1999-01-26 | Bio-Rad Laboratories, Inc. | Fluorescence imaging instrument utilizing fish |
| EP0879297A4 (fr) * | 1995-12-22 | 2003-04-16 | Biorad Lab Inc | Procede permettant de mener un hybridation in situ par fluorescence a parametres multiples et appareil correspondant |
-
1997
- 1997-08-25 US US08/917,213 patent/US5834203A/en not_active Expired - Lifetime
-
1998
- 1998-08-24 EP EP98306743A patent/EP0899558B1/fr not_active Revoked
- 1998-08-24 DE DE69809161T patent/DE69809161T2/de not_active Revoked
- 1998-08-24 ES ES98306743T patent/ES2186097T3/es not_active Expired - Lifetime
- 1998-08-24 EP EP02001413A patent/EP1207376A1/fr not_active Withdrawn
- 1998-08-24 DE DE29824467U patent/DE29824467U1/de not_active Expired - Lifetime
- 1998-08-25 IL IL12591598A patent/IL125915A/en not_active IP Right Cessation
- 1998-08-25 JP JP10279276A patent/JP3130508B2/ja not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| EP0899558A2 (fr) | 1999-03-03 |
| US5834203A (en) | 1998-11-10 |
| IL125915A0 (en) | 1999-04-11 |
| DE69809161D1 (de) | 2002-12-12 |
| JP3130508B2 (ja) | 2001-01-31 |
| ES2186097T3 (es) | 2003-05-01 |
| EP1207376A1 (fr) | 2002-05-22 |
| DE69809161T2 (de) | 2003-03-20 |
| EP0899558A3 (fr) | 1999-09-29 |
| DE29824467U1 (de) | 2001-03-29 |
| EP0899558B1 (fr) | 2002-11-06 |
| JPH11166893A (ja) | 1999-06-22 |
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