WO2002022883A1 - Indicateurs de transfert d'energie de fluorescence combinatoire et utilisations associees - Google Patents

Indicateurs de transfert d'energie de fluorescence combinatoire et utilisations associees Download PDF

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Publication number
WO2002022883A1
WO2002022883A1 PCT/US2001/028967 US0128967W WO0222883A1 WO 2002022883 A1 WO2002022883 A1 WO 2002022883A1 US 0128967 W US0128967 W US 0128967W WO 0222883 A1 WO0222883 A1 WO 0222883A1
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WO
WIPO (PCT)
Prior art keywords
composition
nucleic acid
oligonucleotide
matter
fluorophore
Prior art date
Application number
PCT/US2001/028967
Other languages
English (en)
Inventor
Jingyue Ju
Zengmin Li
Anthony Tong
James J. Russo
Original Assignee
The Trustees Of Columbia University In The City Of New York
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/658,077 external-priority patent/US6627748B1/en
Application filed by The Trustees Of Columbia University In The City Of New York filed Critical The Trustees Of Columbia University In The City Of New York
Priority to CA002421582A priority Critical patent/CA2421582A1/fr
Priority to AU8911101A priority patent/AU8911101A/xx
Priority to JP2002527323A priority patent/JP2004508838A/ja
Priority to EP01968905A priority patent/EP1322785A4/fr
Priority to US10/380,256 priority patent/US20060057565A1/en
Publication of WO2002022883A1 publication Critical patent/WO2002022883A1/fr

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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y20/00—Nanooptics, e.g. quantum optics or photonic crystals
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y10/00—Nanotechnology for information processing, storage or transmission, e.g. quantum computing or single electron logic
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y5/00—Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures

Definitions

  • This invention further provides the instant composition of matter, wherein m is .
  • This invention further provides the instant composition of matter, wherein m is .
  • This invention further provides the instant methods, wherein the isolation-permitting moiety is attached to the dideoxynucleotide via a linker molecule.
  • This invention further provides the instant methods, ' wherein the linker molecule has the structure:
  • spacers are advantageous in several aspects: (i) the spacer will not hybridize to any sequences within the DNA template and therefore false priming is avoided; (ii) the linkage of the spacer maintains the natural nucleic acid phosphate functionality, which avoids possible anomalies in electrophoretic mobility; and (iii) the elimination of the aromatic base groups on the deoxyribose rings in the spacer may reduce the likelihood of fluorescence quenching.
  • Primer 4L 3 ' -atagtagacctgggaaaagg-5 '
  • Template B 5 ' -tacactttgtgaacgccttctgtctgagcacccaGaattagaacatatca tctggacccttttccAgcacaccctgcagaatgagtatgaactcatgaga-3 ' (wild type) (SEQ ID NO: 16)
  • CFET tags can be used in combination with single chromophore/fluorophore tags and tags with multiple chromophores/fluorophores where no FET occurs.
  • fluorophores could be quantum dots, luminescent molecules of fluorescent dyes.
  • each tag could be used to detect a different SNP using the exemplified assays.

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  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Nanotechnology (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Wood Science & Technology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Medical Informatics (AREA)
  • Mathematical Physics (AREA)
  • Theoretical Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Optics & Photonics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)

Abstract

L'invention concerne un indicateur de transfert d'énergie de fluorescence combinatoire comprenant plusieurs molécules fluorescentes, un ou plusieurs donneurs de transfert d'énergie et un ou plusieurs accepteurs de transfert d'énergie, liés via un squelette moléculaire sur lequel les molécules fluorescentes sont espacées afin de produire une signature unique par émission de fluorescence. L'invention concerne aussi l'utilisation de ces indicateurs dans des analyses à composants multiples, y compris dans des analyses biologiques multiplex.
PCT/US2001/028967 2000-09-11 2001-09-11 Indicateurs de transfert d'energie de fluorescence combinatoire et utilisations associees WO2002022883A1 (fr)

Priority Applications (5)

Application Number Priority Date Filing Date Title
CA002421582A CA2421582A1 (fr) 2000-09-11 2001-09-11 Indicateurs de transfert d'energie de fluorescence combinatoire et utilisations associees
AU8911101A AU8911101A (en) 2000-09-11 2001-09-11 Combinatorial fluorescence energy transfer tags and uses thereof
JP2002527323A JP2004508838A (ja) 2000-09-11 2001-09-11 組合せ蛍光エネルギー移動タグ及びそれらの使用
EP01968905A EP1322785A4 (fr) 2000-09-11 2001-09-11 Indicateurs de transfert d'energie de fluorescence combinatoire et utilisations associees
US10/380,256 US20060057565A1 (en) 2000-09-11 2001-09-11 Combinatorial fluorescence energy transfer tags and uses thereof

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US09/658,077 US6627748B1 (en) 2000-09-11 2000-09-11 Combinatorial fluorescence energy transfer tags and their applications for multiplex genetic analyses
US09/658,077 2000-09-11
US30915601P 2001-07-31 2001-07-31
US60/309,156 2001-07-31

Publications (1)

Publication Number Publication Date
WO2002022883A1 true WO2002022883A1 (fr) 2002-03-21

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ID=26976642

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/028967 WO2002022883A1 (fr) 2000-09-11 2001-09-11 Indicateurs de transfert d'energie de fluorescence combinatoire et utilisations associees

Country Status (5)

Country Link
EP (1) EP1322785A4 (fr)
JP (1) JP2004508838A (fr)
AU (1) AU8911101A (fr)
CA (1) CA2421582A1 (fr)
WO (1) WO2002022883A1 (fr)

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US6664079B2 (en) 2000-10-06 2003-12-16 The Trustees Of Columbia University In The City Of New York Massive parallel method for decoding DNA and RNA
JP2004520031A (ja) * 2001-01-09 2004-07-08 クイーンメリー アンド ウエストフィールド カレッジ 薬学的活性物質に潜在性を提供する方法
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US6627748B1 (en) 2000-09-11 2003-09-30 The Trustees Of Columbia University In The City Of New York Combinatorial fluorescence energy transfer tags and their applications for multiplex genetic analyses
US10407459B2 (en) 2000-10-06 2019-09-10 The Trustees Of Columbia University In The City Of New York Massive parallel method for decoding DNA and RNA
US6664079B2 (en) 2000-10-06 2003-12-16 The Trustees Of Columbia University In The City Of New York Massive parallel method for decoding DNA and RNA
US10435742B2 (en) 2000-10-06 2019-10-08 The Trustees Of Columbia University In The City Of New York Massive parallel method for decoding DNA and RNA
US10407458B2 (en) 2000-10-06 2019-09-10 The Trustees Of Columbia University In The City Of New York Massive parallel method for decoding DNA and RNA
US9868985B2 (en) 2000-10-06 2018-01-16 The Trustees Of Columbia University In The City Of New York Massive parallel method for decoding DNA and RNA
US7345159B2 (en) 2000-10-06 2008-03-18 The Trustees Of Columbia University In The City Of New York Massive parallel method for decoding DNA and RNA
US10633700B2 (en) 2000-10-06 2020-04-28 The Trustees Of Columbia University In The City Of New York Massive parallel method for decoding DNA and RNA
US10428380B2 (en) 2000-10-06 2019-10-01 The Trustees Of Columbia University In The City Of New York Massive parallel method for decoding DNA and RNA
US7635578B2 (en) 2000-10-06 2009-12-22 The Trustees Of Columbia University In The City Of New York Massive parallel method for decoding DNA and RNA
US7713698B2 (en) 2000-10-06 2010-05-11 The Trustees Of Columbia University In The City Of New York Massive parallel method for decoding DNA and RNA
US10570446B2 (en) 2000-10-06 2020-02-25 The Trustee Of Columbia University In The City Of New York Massive parallel method for decoding DNA and RNA
US7790869B2 (en) 2000-10-06 2010-09-07 The Trustees Of Columbia University In The City Of New York Massive parallel method for decoding DNA and RNA
US10669582B2 (en) 2000-10-06 2020-06-02 The Trustees Of Columbia University In The City Of New York Massive parallel method for decoding DNA and RNA
US8088575B2 (en) 2000-10-06 2012-01-03 The Trustees Of Columbia University In The City Of New York Massive parallel method for decoding DNA and RNA
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US9718852B2 (en) 2000-10-06 2017-08-01 The Trustees Of Columbia University In The City Of New York Massive parallel method for decoding DNA and RNA
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US9708358B2 (en) 2000-10-06 2017-07-18 The Trustees Of Columbia University In The City Of New York Massive parallel method for decoding DNA and RNA
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US10577652B2 (en) 2000-10-06 2020-03-03 The Trustees Of Columbia University In The City Of New York Massive parallel method for decoding DNA and RNA
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US10669577B2 (en) 2000-10-06 2020-06-02 The Trustees Of Columbia University In The City Of New York Massive parallel method for decoding DNA and RNA
US10457984B2 (en) 2000-10-06 2019-10-29 The Trustees Of Columbia University In The City Of New York Massive parallel method for decoding DNA and RNA
JP2004520031A (ja) * 2001-01-09 2004-07-08 クイーンメリー アンド ウエストフィールド カレッジ 薬学的活性物質に潜在性を提供する方法
US10519496B2 (en) 2001-12-04 2019-12-31 Illumina Cambridge Limited Labelled nucleotides
US9605310B2 (en) 2001-12-04 2017-03-28 Illumina Cambridge Limited Labelled nucleotides
US9121062B2 (en) 2001-12-04 2015-09-01 Illumina Cambridge Limited Labelled nucleotides
US9410200B2 (en) 2001-12-04 2016-08-09 Illumina Cambridge Limited Labelled nucleotides
US9388463B2 (en) 2001-12-04 2016-07-12 Illumina Cambridge Limited Labelled nucleotides
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