IE47021B1 - Method of purifying an insulin-containing aqueous ethanolic raw extract from pancreas glands - Google Patents
Method of purifying an insulin-containing aqueous ethanolic raw extract from pancreas glandsInfo
- Publication number
- IE47021B1 IE47021B1 IE1342/78A IE134278A IE47021B1 IE 47021 B1 IE47021 B1 IE 47021B1 IE 1342/78 A IE1342/78 A IE 1342/78A IE 134278 A IE134278 A IE 134278A IE 47021 B1 IE47021 B1 IE 47021B1
- Authority
- IE
- Ireland
- Prior art keywords
- insulin
- ethanol
- extract
- gel
- concentration
- Prior art date
Links
- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 title claims abstract description 157
- 102000004877 Insulin Human genes 0.000 title claims abstract description 78
- 108090001061 Insulin Proteins 0.000 title claims abstract description 78
- 229940125396 insulin Drugs 0.000 title claims abstract description 77
- 239000000284 extract Substances 0.000 title claims abstract description 32
- 238000000034 method Methods 0.000 title claims abstract description 25
- 210000004907 gland Anatomy 0.000 title claims abstract description 13
- 210000000496 pancreas Anatomy 0.000 title claims abstract description 12
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 64
- 238000002523 gelfiltration Methods 0.000 claims abstract description 10
- 238000000926 separation method Methods 0.000 claims abstract description 9
- 239000013078 crystal Substances 0.000 claims abstract description 4
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 claims description 6
- 230000007717 exclusion Effects 0.000 claims description 4
- 229920002307 Dextran Polymers 0.000 claims description 3
- BRLQWZUYTZBJKN-UHFFFAOYSA-N Epichlorohydrin Chemical compound ClCC1CO1 BRLQWZUYTZBJKN-UHFFFAOYSA-N 0.000 claims description 3
- 125000002791 glucosyl group Chemical group C1([C@H](O)[C@@H](O)[C@H](O)[C@H](O1)CO)* 0.000 claims description 3
- -1 hydroxypropyl groups Chemical group 0.000 claims description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 3
- 238000002425 crystallisation Methods 0.000 claims description 2
- 230000008025 crystallization Effects 0.000 claims description 2
- 239000000126 substance Substances 0.000 abstract description 11
- 229920005654 Sephadex Polymers 0.000 abstract description 5
- 239000012507 Sephadex™ Substances 0.000 abstract description 5
- 102000004169 proteins and genes Human genes 0.000 abstract description 5
- 108090000623 proteins and genes Proteins 0.000 abstract description 5
- 238000004040 coloring Methods 0.000 abstract description 4
- 238000001816 cooling Methods 0.000 abstract description 4
- 239000011324 bead Substances 0.000 abstract 1
- 239000007788 liquid Substances 0.000 abstract 1
- 238000000746 purification Methods 0.000 description 13
- 239000012535 impurity Substances 0.000 description 7
- 150000002500 ions Chemical class 0.000 description 6
- 238000011282 treatment Methods 0.000 description 6
- 239000007791 liquid phase Substances 0.000 description 5
- 238000001223 reverse osmosis Methods 0.000 description 5
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 4
- 239000012141 concentrate Substances 0.000 description 4
- 238000004810 partition chromatography Methods 0.000 description 4
- 238000002360 preparation method Methods 0.000 description 4
- 239000007787 solid Substances 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- 239000002904 solvent Substances 0.000 description 4
- 125000004432 carbon atom Chemical group C* 0.000 description 3
- 238000001556 precipitation Methods 0.000 description 3
- 150000001298 alcohols Chemical class 0.000 description 2
- 229910021529 ammonia Inorganic materials 0.000 description 2
- 239000003125 aqueous solvent Substances 0.000 description 2
- 150000001735 carboxylic acids Chemical class 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 238000005342 ion exchange Methods 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 238000011084 recovery Methods 0.000 description 2
- 230000008961 swelling Effects 0.000 description 2
- 239000004475 Arginine Substances 0.000 description 1
- 108010076181 Proinsulin Proteins 0.000 description 1
- 241000282887 Suidae Species 0.000 description 1
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 1
- PTFCDOFLOPIGGS-UHFFFAOYSA-N Zinc dication Chemical compound [Zn+2] PTFCDOFLOPIGGS-UHFFFAOYSA-N 0.000 description 1
- 239000008351 acetate buffer Substances 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 108700022849 desamido- insulin Proteins 0.000 description 1
- 239000000539 dimer Substances 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000010828 elution Methods 0.000 description 1
- 239000000469 ethanolic extract Substances 0.000 description 1
- 125000004494 ethyl ester group Chemical group 0.000 description 1
- 239000012467 final product Substances 0.000 description 1
- 239000012847 fine chemical Substances 0.000 description 1
- 239000007863 gel particle Substances 0.000 description 1
- 239000004026 insulin derivative Substances 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 229910021645 metal ion Inorganic materials 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000002808 molecular sieve Substances 0.000 description 1
- 150000007530 organic bases Chemical class 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 238000005192 partition Methods 0.000 description 1
- 239000002243 precursor Substances 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 230000006920 protein precipitation Effects 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- URGAHOPLAPQHLN-UHFFFAOYSA-N sodium aluminosilicate Chemical compound [Na+].[Al+3].[O-][Si]([O-])=O.[O-][Si]([O-])=O URGAHOPLAPQHLN-UHFFFAOYSA-N 0.000 description 1
- 235000011149 sulphuric acid Nutrition 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 230000001502 supplementing effect Effects 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/62—Insulins
- C07K14/625—Extraction from natural sources
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- Genetics & Genomics (AREA)
- Gastroenterology & Hepatology (AREA)
- Toxicology (AREA)
- Endocrinology (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Zoology (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Diabetes (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DK773114A DK311477A (da) | 1977-07-08 | 1977-07-08 | Fremgangsmaade ved fremstilling af rent insulin |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| IE781342L IE781342L (en) | 1979-01-08 |
| IE47021B1 true IE47021B1 (en) | 1983-11-30 |
Family
ID=8119244
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| IE1342/78A IE47021B1 (en) | 1977-07-08 | 1978-07-04 | Method of purifying an insulin-containing aqueous ethanolic raw extract from pancreas glands |
Country Status (6)
| Country | Link |
|---|---|
| EP (1) | EP0000439B1 (da) |
| AT (1) | AT368695B (da) |
| DE (1) | DE2860997D1 (da) |
| DK (1) | DK311477A (da) |
| IE (1) | IE47021B1 (da) |
| IT (1) | IT1096920B (da) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DK366380A (da) * | 1980-08-28 | 1982-03-01 | Novo Industri As | Anvendelsen af grp og salte deraf |
| DE3726655A1 (de) * | 1987-08-11 | 1989-02-23 | Hoechst Ag | Verfahren zur isolierung basischer proteine aus proteingemischen, welche solche basischen proteine enthalten |
| ES2557559T3 (es) | 2010-09-16 | 2016-01-27 | F. Hoffmann-La Roche Ag | Proceso para preparar un derivado de ciclohexanocarbonitrilo |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE2212695A1 (de) * | 1972-03-16 | 1973-09-20 | Hoechst Ag | Verfahren zur reinigung von insulin und insulinderivaten |
| US3876623A (en) * | 1973-05-09 | 1975-04-08 | Lilly Co Eli | Process for purifying insulin |
| GB1581824A (en) * | 1976-01-16 | 1980-12-31 | Leo Sa Lab | Preparation of insulin |
-
1977
- 1977-07-08 DK DK773114A patent/DK311477A/da not_active Application Discontinuation
-
1978
- 1978-07-04 IE IE1342/78A patent/IE47021B1/en unknown
- 1978-07-07 DE DE7878300127T patent/DE2860997D1/de not_active Expired
- 1978-07-07 IT IT25460/78A patent/IT1096920B/it active
- 1978-07-07 AT AT0494578A patent/AT368695B/de not_active IP Right Cessation
- 1978-07-07 EP EP78300127A patent/EP0000439B1/en not_active Expired
Also Published As
| Publication number | Publication date |
|---|---|
| IE781342L (en) | 1979-01-08 |
| IT1096920B (it) | 1985-08-26 |
| EP0000439B1 (en) | 1981-09-02 |
| DK311477A (da) | 1979-01-09 |
| DE2860997D1 (en) | 1981-11-26 |
| AT368695B (de) | 1982-10-25 |
| IT7825460A0 (it) | 1978-07-07 |
| EP0000439A1 (en) | 1979-01-24 |
| ATA494578A (de) | 1982-03-15 |
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