HUP0302600A2 - Eljárás petefészektüszők in vitro tenyésztésére - Google Patents

Eljárás petefészektüszők in vitro tenyésztésére

Info

Publication number
HUP0302600A2
HUP0302600A2 HU0302600A HUP0302600A HUP0302600A2 HU P0302600 A2 HUP0302600 A2 HU P0302600A2 HU 0302600 A HU0302600 A HU 0302600A HU P0302600 A HUP0302600 A HU P0302600A HU P0302600 A2 HUP0302600 A2 HU P0302600A2
Authority
HU
Hungary
Prior art keywords
follicle
ovarian follicles
vitro culture
attachment
oil
Prior art date
Application number
HU0302600A
Other languages
English (en)
Inventor
Rita Cortvrindt
Johan Smitz
Original Assignee
Vrije Universiteit Brussel
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Vrije Universiteit Brussel filed Critical Vrije Universiteit Brussel
Publication of HUP0302600A2 publication Critical patent/HUP0302600A2/hu
Publication of HUP0302600A3 publication Critical patent/HUP0302600A3/hu

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0608—Germ cells
    • C12N5/0609—Oocytes, oogonia
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
    • C12N2500/24—Iron; Fe chelators; Transferrin
    • C12N2500/25—Insulin-transferrin; Insulin-transferrin-selenium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/11—Epidermal growth factor [EGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/31—Pituitary sex hormones, e.g. follicle-stimulating hormone [FSH], luteinising hormone [LH]; Chorionic gonadotropins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/24—Genital tract cells, non-germinal cells from gonads
    • C12N2502/243—Cells of the female genital tract, non-germinal ovarian cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2517/00—Cells related to new breeds of animals
    • C12N2517/10—Conditioning of cells for in vitro fecondation or nuclear transfer

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biochemistry (AREA)
  • Developmental Biology & Embryology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Mushroom Cultivation (AREA)

Abstract

A találmány biológiai meghatározási célokra emlős petefészektüszők invitro tenyésztésére alkalmas eljárásra vonatkozik, amely a következőlépéseket tartalmazza: - megfelelő tartály biztosítása az in vitrotenyészet számára - egy emlős petefészkéből tüsző kiválasztása, ahol atüsző legalább - egy hüvely- vagy pre-hüvelysejtet, - egygranulózasejtet és - egy oocitát tartalmaz, - egy adott esetbenvégzett első tenyésztési lépés, amelyhez olyan, kapcsolódást gátlóelső közeget használnak, amely olajtól mentes, - egy másodiktenyésztési lépés, amelyhez egy olyan, kapcsolódást elősegítő másodikközeget használnak, amely olajtól mentes, és - az érett tüszőkinyerése. Ó
HU0302600A 2000-12-13 2001-12-11 Method for in vitro culture of ovarian follicles HUP0302600A3 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP00870300A EP1215280A1 (en) 2000-12-13 2000-12-13 Method for in vitro culture of ovarian follicles
PCT/BE2001/000209 WO2002048316A1 (en) 2000-12-13 2001-12-11 Method for in vitro culture of ovarian follicles

Publications (2)

Publication Number Publication Date
HUP0302600A2 true HUP0302600A2 (hu) 2003-11-28
HUP0302600A3 HUP0302600A3 (en) 2004-10-28

Family

ID=8175874

Family Applications (1)

Application Number Title Priority Date Filing Date
HU0302600A HUP0302600A3 (en) 2000-12-13 2001-12-11 Method for in vitro culture of ovarian follicles

Country Status (18)

Country Link
US (1) US20040053407A1 (hu)
EP (2) EP1215280A1 (hu)
JP (1) JP2004514458A (hu)
KR (1) KR20030081350A (hu)
CN (2) CN1916165A (hu)
AT (1) ATE408668T1 (hu)
AU (2) AU2002224669B2 (hu)
CA (1) CA2429401A1 (hu)
CZ (1) CZ20031607A3 (hu)
DE (1) DE60135869D1 (hu)
DK (1) DK1341901T3 (hu)
HU (1) HUP0302600A3 (hu)
IL (1) IL156375A0 (hu)
NZ (1) NZ525925A (hu)
PL (1) PL362063A1 (hu)
RU (1) RU2286384C2 (hu)
WO (1) WO2002048316A1 (hu)
ZA (1) ZA200303864B (hu)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2005292362B2 (en) * 2004-09-30 2012-05-31 Merck Serono Sa Use of IL-17- for maturation of oocytes
AU2005292374A1 (en) * 2004-09-30 2006-04-13 Laboratoires Serono Sa Use of pregnancy specific glycoprotein for maturation of oocytes
US20070020755A1 (en) * 2005-07-07 2007-01-25 Teresa Woodruff Stage specific follicle maturation systems
WO2007103177A2 (en) * 2006-03-03 2007-09-13 O2Cyte, Llc Manipulation of oxygen tension during in vitro follicle culture
KR100793680B1 (ko) 2006-09-15 2008-01-10 재단법인서울대학교산학협력재단 새로운 오일 없는 수정란 배양 시스템
CN100999722B (zh) * 2007-01-08 2011-08-03 安徽省立医院 卵巢组织块内不成熟卵母细胞的体外培养成熟方法
CN100999723B (zh) * 2007-01-08 2011-08-31 安徽省立医院 经促排分离的或从卵巢组织内抽吸获得的未成熟卵母细胞体外培养成熟方法
CN100999724B (zh) * 2007-01-08 2011-04-20 安徽省立医院 应用在动物不成熟卵母细胞体外培养成熟中的饲养细胞
EP3275311B1 (en) * 2015-03-26 2020-12-30 Nippon Suisan Kaisha, Ltd. Method of maintaining ovaries of marine fish, method of adjusting culture medium, and method of producing eggs or fertilized eggs of marine fish
KR101788968B1 (ko) * 2016-04-14 2017-10-23 서울대학교산학협력단 안지오텐신 ⅱ 수용체 작용제를 이용한 복수 난포 배양 방법
RU2707252C1 (ru) * 2018-08-02 2019-11-25 общество с ограниченной ответственностью "АРТ БиоВет" Способ заморозки ооцитов крупного рогатого скота
CN109852533B (zh) * 2019-04-02 2022-02-25 泰山医学院 一种动物卵巢的高效率卵母细胞分离收集装置
CN110129402B (zh) * 2019-04-15 2023-08-01 宁夏医科大学 一种Rho-ROCK信号通路抑制剂Y27632对小鼠卵泡体外发育影响的研究方法
CN112980773B (zh) * 2021-03-15 2023-11-17 中山大学附属第六医院 一种体外培养小鼠次级卵泡的方法
CN113462635A (zh) * 2021-07-27 2021-10-01 河北农业大学 一种绵羊卵巢颗粒细胞分离、培养及鉴定方法
KR102690765B1 (ko) * 2021-12-16 2024-08-05 고려대학교 산학협력단 클로토 단백질 및 n-아세틸시스테인을 포함하는 난소 동결보존용 조성물
WO2024024742A1 (ja) * 2022-07-25 2024-02-01 国立大学法人京都大学 霊長類の胎児卵巣細胞から卵胞を誘導する体外培養法

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4987080A (en) * 1987-11-03 1991-01-22 Grob Howard S Method for in vitro maturation of oocytes

Also Published As

Publication number Publication date
CZ20031607A3 (cs) 2003-11-12
RU2286384C2 (ru) 2006-10-27
EP1215280A1 (en) 2002-06-19
DK1341901T3 (da) 2009-01-26
NZ525925A (en) 2005-12-23
AU2466902A (en) 2002-06-24
JP2004514458A (ja) 2004-05-20
ZA200303864B (en) 2004-04-05
HUP0302600A3 (en) 2004-10-28
DE60135869D1 (de) 2008-10-30
EP1341901B8 (en) 2008-12-24
EP1341901B1 (en) 2008-09-17
EP1341901A1 (en) 2003-09-10
WO2002048316A1 (en) 2002-06-20
CN1916165A (zh) 2007-02-21
CA2429401A1 (en) 2002-06-20
PL362063A1 (en) 2004-10-18
AU2002224669B2 (en) 2007-02-01
IL156375A0 (en) 2004-01-04
KR20030081350A (ko) 2003-10-17
US20040053407A1 (en) 2004-03-18
CN1481437A (zh) 2004-03-10
ATE408668T1 (de) 2008-10-15
CN100489089C (zh) 2009-05-20

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Legal Events

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FD9A Lapse of provisional protection due to non-payment of fees