HUE033504T2 - Szív szövetbõl kinyert decellularizált extracelluláris mátrixot tartalmazó kompozíciók - Google Patents
Szív szövetbõl kinyert decellularizált extracelluláris mátrixot tartalmazó kompozíciók Download PDFInfo
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- HUE033504T2 HUE033504T2 HUE09818438A HUE09818438A HUE033504T2 HU E033504 T2 HUE033504 T2 HU E033504T2 HU E09818438 A HUE09818438 A HU E09818438A HU E09818438 A HUE09818438 A HU E09818438A HU E033504 T2 HUE033504 T2 HU E033504T2
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- Neurosurgery (AREA)
Claims (8)
- Szív szövetből kinyert decellularízált: extraceiluíáris mátrixot tartalmazó kompozíciók S zabadal m i i génypont ok1. Egy folyékony kompozíció, amely szív szövetből származó deeeliularizált extraceiiulárís mátrixot tartalmaz, ahol a kompozíció egy tts katéteren keresztül a katéter eltömítése nélkül történő bejuttatásra alkalmas, ahol a deeellulari/áh exiracelluláris mátrixot egy az alábbiakat tartalmazó eljárással állíthatjuk elő: a) feldolgozónk egy exiraeelluláris mátrix komponenst és egy nem-extraeeílníáris mátrix komponenst tartalmazó szív szöveti mintát a nem-extracellulárís mátrix komponens eltávolítására és így deceilufarizáft extraceiluíáris mátrix kinyerésére; b.) a deeeliularizált extraeelioláris mátrixot iiofilízáljuk és porrá alakítjuk; e) egy a deeeliularizált- extraceiiulárís mátrix port egy1 folyadékban tartalmazó kompozíciót készítünk; d) .a e) lépésben kapott deeeliularizált extraceiluíáris mátrixot egy mátrix lebontó enzimmel 1 -6 pM-érték mellett emésztjük; és e) a d) lépésben kapott., emésztett deeeliularizált cxlíacelluiáris mátrix pM-ját semlegesítjük a folyékony kompozíció előállítására; ahol a folyékony kompozíció testhőmérsékleten géllé aiakul.
- 2. Az 1, igénypont szerinti kompozíció, ahoi az említett folyékony kompozíció továbbá sejteket is tartalmaz. 3. A 2, igénypont szerinti kompozíció, ahol a sejtek exogen sejtek és az alábbi csoportból választottak vagy származtathatók ; indukált pluripotens őssejtek, kardiomhxiták, kardiáim progenitor sejtek, miohlasztok, csontváz sejtek, flhrohlasztok, endotefiáíls sejtek és nem-humán embrió őssejtek.
- 4. Az i. igénypont szerinti kompozíció, ahol az említett kompozíció továbbá egy szintetikus vagy természetben előforduló polimert tana lm az,
- 5. Az 1, igénypont szerinti kompozíció, ahol az említett kompozíció továbbá egy exogén terápiás szert is tartalmaz. ó. Az l. igénypont szerinti kompozíció, ahol a folyékony kompozíció egy neutralizálí oldat
- 7. Az í, igénypont 'szerinti kompozíció, ahol a katéter tője 27-es vagy ennél nagyobb űrméretű, 8; Az 1, igénypont szerinti kompozíció, ahol az extraeeiiuiáris mátrix koncentrációja a folyadék ban 1 és 20 mg/mi közötti.
- 9. Eljárás egy szív szövetből származó decelíuiarizált extraeeiiuiáris mátrix port tartalmazó kompozíció előállítására, amely eljárás az. alábbi lépéseket tartalmazza: a) egy extraeeiiuiáris mátrix komponenst és egy nem-extracellniárts mátrix komponenst tartalmazó szív szöveti mintái nátrium-dodeei 1-szu 1 lattal dolgozunk fel a nem-extraceliuláris mátrix komponens eltávolítására ás így dece Ily fari záít extraeeiiuiáris mátrix kinyerésére; b) a decelíuiarizált extraeeiiuiáris mátrixot líofílízáljuk és porrá alakítjuk; c) egy a decelíuiarizált extraeeiiuiáris mátrix port egy folyadékban tartalmazó kompozíciót készítünk; d) a c) lépésben kapott deceHuiarizált extraeeiiuiáris mátrixot egy mátrix lebontó enzimmel 1-6 pH-éríék mellett emésztjük; és e) a d) lépésben kapott, emésztett decelíuiarizált extraeeiiuiáris mátrix pH-ját semlegesítjük, 10. A 9. igénypont szerimi eljárás, amely további lépésként tartalmazza a deeellvilarizált extraeel-lulárís mátrix port tartalmazó folyékony kompozíció előállítását Π. A 10. igénypont szerinti eljárás, amely további lépésként tartalmazza a folyékony kompozíció pH-j ának boáiIttasát 12, í\ 10, igénypont szerinti eljárás, ahol az említett folyékony kompozíció a bejuttatás után gél formájúvá alakul. 13, A 10, igénypont szerinti eljárás, amely további lépésként tartalmazza a folyékony kompozíció hőmérsékletének beállítását.
- 14, Az 1-8. igénypontok bármelyike szerinti folyékony kompozíció terápiás alkalmazásra.
- 15, Az 1-8, igénypontok bármelyike szerinti folyékony kompozíció arriimía, míokardiális infarktus vagy egyéb, defektiv, beteg, károsodott vagy íszkémiás szív szövet, kezelésénél történő alkalmazásra.
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| US10133208P | 2008-09-30 | 2008-09-30 |
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| HUE033504T2 true HUE033504T2 (hu) | 2017-12-28 |
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| EP (2) | EP3246038B1 (hu) |
| CN (2) | CN102227225B (hu) |
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| CA (1) | CA2741770C (hu) |
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| GB (1) | GB2476624B (hu) |
| HU (1) | HUE033504T2 (hu) |
| PL (1) | PL2349292T3 (hu) |
| WO (1) | WO2010039823A2 (hu) |
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| CA2741770A1 (en) | 2010-04-08 |
| GB2476624A (en) | 2011-06-29 |
| US20110189140A1 (en) | 2011-08-04 |
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| US20210260134A1 (en) | 2021-08-26 |
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| PL2349292T3 (pl) | 2017-09-29 |
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| GB2476624B (en) | 2013-07-10 |
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| GB201107203D0 (en) | 2011-06-15 |
| CN106178118A (zh) | 2016-12-07 |
| WO2010039823A8 (en) | 2010-05-20 |
| WO2010039823A2 (en) | 2010-04-08 |
| EP2349292B1 (en) | 2017-04-12 |
| AU2009298560A1 (en) | 2010-04-08 |
| US20130251687A1 (en) | 2013-09-26 |
| EP2349292A4 (en) | 2012-07-11 |
| EP3246038B1 (en) | 2021-04-07 |
| CA2741770C (en) | 2018-01-23 |
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