HU202581B - Process for producing valuable protein materials with utilizing recombinant dns of bomby mori polyhedrosis virus - Google Patents
Process for producing valuable protein materials with utilizing recombinant dns of bomby mori polyhedrosis virus Download PDFInfo
- Publication number
- HU202581B HU202581B HU852434A HU243485A HU202581B HU 202581 B HU202581 B HU 202581B HU 852434 A HU852434 A HU 852434A HU 243485 A HU243485 A HU 243485A HU 202581 B HU202581 B HU 202581B
- Authority
- HU
- Hungary
- Prior art keywords
- dna
- gene
- bmnpv
- structural gene
- plasmid
- Prior art date
Links
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 172
- 238000000034 method Methods 0.000 title claims abstract description 43
- 102000004169 proteins and genes Human genes 0.000 title claims abstract description 37
- 241000700605 Viruses Species 0.000 title abstract description 35
- 239000000463 material Substances 0.000 title description 36
- 230000008569 process Effects 0.000 title description 11
- 239000012634 fragment Substances 0.000 claims abstract description 84
- 238000004519 manufacturing process Methods 0.000 claims abstract description 43
- 238000011144 upstream manufacturing Methods 0.000 claims abstract description 32
- 108700039691 Genetic Promoter Regions Proteins 0.000 claims abstract description 17
- 239000013612 plasmid Substances 0.000 claims description 79
- 241000255789 Bombyx mori Species 0.000 claims description 30
- 108091008146 restriction endonucleases Proteins 0.000 claims description 16
- 241000255791 Bombyx Species 0.000 claims description 9
- 238000001890 transfection Methods 0.000 claims description 4
- 241000701366 unidentified nuclear polyhedrosis viruses Species 0.000 claims description 3
- 239000013603 viral vector Substances 0.000 claims description 3
- 238000006467 substitution reaction Methods 0.000 claims description 2
- 108020004414 DNA Proteins 0.000 abstract description 84
- 241000409811 Bombyx mori nucleopolyhedrovirus Species 0.000 abstract description 65
- 239000000126 substance Substances 0.000 abstract description 12
- 108091081024 Start codon Proteins 0.000 abstract description 9
- 238000005215 recombination Methods 0.000 abstract description 8
- 230000006798 recombination Effects 0.000 abstract description 8
- 102000053602 DNA Human genes 0.000 abstract description 3
- 230000001902 propagating effect Effects 0.000 abstract description 2
- 210000004748 cultured cell Anatomy 0.000 abstract 1
- 210000004027 cell Anatomy 0.000 description 27
- 239000000047 product Substances 0.000 description 22
- 108020005202 Viral DNA Proteins 0.000 description 18
- 239000000243 solution Substances 0.000 description 15
- 108091028043 Nucleic acid sequence Proteins 0.000 description 12
- 230000001174 ascending effect Effects 0.000 description 12
- 239000002609 medium Substances 0.000 description 11
- 239000013598 vector Substances 0.000 description 11
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 10
- 238000000338 in vitro Methods 0.000 description 9
- 238000003776 cleavage reaction Methods 0.000 description 8
- 230000007017 scission Effects 0.000 description 8
- 230000014616 translation Effects 0.000 description 8
- 241000588724 Escherichia coli Species 0.000 description 7
- 241001646716 Escherichia coli K-12 Species 0.000 description 7
- 238000013519 translation Methods 0.000 description 7
- JUJWROOIHBZHMG-UHFFFAOYSA-N Pyridine Chemical compound C1=CC=NC=C1 JUJWROOIHBZHMG-UHFFFAOYSA-N 0.000 description 6
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 6
- 238000004113 cell culture Methods 0.000 description 6
- 238000002360 preparation method Methods 0.000 description 6
- 238000011160 research Methods 0.000 description 6
- 238000003786 synthesis reaction Methods 0.000 description 6
- 102000004190 Enzymes Human genes 0.000 description 5
- 108090000790 Enzymes Proteins 0.000 description 5
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 5
- 108020004511 Recombinant DNA Proteins 0.000 description 5
- 125000003275 alpha amino acid group Chemical group 0.000 description 5
- 210000001124 body fluid Anatomy 0.000 description 5
- 239000010839 body fluid Substances 0.000 description 5
- 238000010367 cloning Methods 0.000 description 5
- 239000002299 complementary DNA Substances 0.000 description 5
- 238000007796 conventional method Methods 0.000 description 5
- 230000000694 effects Effects 0.000 description 5
- 238000010353 genetic engineering Methods 0.000 description 5
- 239000010410 layer Substances 0.000 description 5
- 108020004999 messenger RNA Proteins 0.000 description 5
- 230000000644 propagated effect Effects 0.000 description 5
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 4
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 4
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 4
- 229930006000 Sucrose Natural products 0.000 description 4
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 4
- 108020005038 Terminator Codon Proteins 0.000 description 4
- 230000027455 binding Effects 0.000 description 4
- 238000006243 chemical reaction Methods 0.000 description 4
- 238000010276 construction Methods 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 4
- 238000001727 in vivo Methods 0.000 description 4
- 208000015181 infectious disease Diseases 0.000 description 4
- 230000001418 larval effect Effects 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- 108090000765 processed proteins & peptides Proteins 0.000 description 4
- 239000005720 sucrose Substances 0.000 description 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- WFDIJRYMOXRFFG-UHFFFAOYSA-N Acetic anhydride Chemical compound CC(=O)OC(C)=O WFDIJRYMOXRFFG-UHFFFAOYSA-N 0.000 description 3
- WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 description 3
- 108020004638 Circular DNA Proteins 0.000 description 3
- 208000003322 Coinfection Diseases 0.000 description 3
- YMWUJEATGCHHMB-UHFFFAOYSA-N Dichloromethane Chemical compound ClCCl YMWUJEATGCHHMB-UHFFFAOYSA-N 0.000 description 3
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 3
- 102000003886 Glycoproteins Human genes 0.000 description 3
- 108090000288 Glycoproteins Proteins 0.000 description 3
- 108010047761 Interferon-alpha Proteins 0.000 description 3
- 102000006992 Interferon-alpha Human genes 0.000 description 3
- 108090000467 Interferon-beta Proteins 0.000 description 3
- 108010021757 Polynucleotide 5'-Hydroxyl-Kinase Proteins 0.000 description 3
- 102000008422 Polynucleotide 5'-hydroxyl-kinase Human genes 0.000 description 3
- 238000002105 Southern blotting Methods 0.000 description 3
- 238000000246 agarose gel electrophoresis Methods 0.000 description 3
- 230000015572 biosynthetic process Effects 0.000 description 3
- 238000005119 centrifugation Methods 0.000 description 3
- 230000029087 digestion Effects 0.000 description 3
- 230000008030 elimination Effects 0.000 description 3
- 238000003379 elimination reaction Methods 0.000 description 3
- 238000000605 extraction Methods 0.000 description 3
- 238000002513 implantation Methods 0.000 description 3
- 108020004707 nucleic acids Proteins 0.000 description 3
- 102000039446 nucleic acids Human genes 0.000 description 3
- 150000007523 nucleic acids Chemical class 0.000 description 3
- 239000002245 particle Substances 0.000 description 3
- UMJSCPRVCHMLSP-UHFFFAOYSA-N pyridine Natural products COC1=CC=CN=C1 UMJSCPRVCHMLSP-UHFFFAOYSA-N 0.000 description 3
- 239000011347 resin Substances 0.000 description 3
- 229920005989 resin Polymers 0.000 description 3
- 239000011780 sodium chloride Substances 0.000 description 3
- 235000019333 sodium laurylsulphate Nutrition 0.000 description 3
- 239000006228 supernatant Substances 0.000 description 3
- 239000000725 suspension Substances 0.000 description 3
- 238000012546 transfer Methods 0.000 description 3
- 230000009466 transformation Effects 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- 125000002103 4,4'-dimethoxytriphenylmethyl group Chemical group [H]C1=C([H])C([H])=C(C([H])=C1[H])C(*)(C1=C([H])C([H])=C(OC([H])([H])[H])C([H])=C1[H])C1=C([H])C([H])=C(OC([H])([H])[H])C([H])=C1[H] 0.000 description 2
- 229920000936 Agarose Polymers 0.000 description 2
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 2
- 108020004705 Codon Proteins 0.000 description 2
- 241000206602 Eukaryota Species 0.000 description 2
- 102000003996 Interferon-beta Human genes 0.000 description 2
- 108010076504 Protein Sorting Signals Proteins 0.000 description 2
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 description 2
- 239000007983 Tris buffer Substances 0.000 description 2
- 238000001042 affinity chromatography Methods 0.000 description 2
- 239000011543 agarose gel Substances 0.000 description 2
- 238000003556 assay Methods 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- 239000000872 buffer Substances 0.000 description 2
- 230000001413 cellular effect Effects 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 230000000120 cytopathologic effect Effects 0.000 description 2
- 238000000354 decomposition reaction Methods 0.000 description 2
- 230000007812 deficiency Effects 0.000 description 2
- 238000000432 density-gradient centrifugation Methods 0.000 description 2
- 239000012153 distilled water Substances 0.000 description 2
- 238000004128 high performance liquid chromatography Methods 0.000 description 2
- 238000009396 hybridization Methods 0.000 description 2
- 230000005764 inhibitory process Effects 0.000 description 2
- 238000003780 insertion Methods 0.000 description 2
- 230000037431 insertion Effects 0.000 description 2
- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 description 2
- 229960001388 interferon-beta Drugs 0.000 description 2
- 238000004255 ion exchange chromatography Methods 0.000 description 2
- 238000002955 isolation Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 239000002808 molecular sieve Substances 0.000 description 2
- 238000002703 mutagenesis Methods 0.000 description 2
- 231100000350 mutagenesis Toxicity 0.000 description 2
- 229940124276 oligodeoxyribonucleotide Drugs 0.000 description 2
- 239000012071 phase Substances 0.000 description 2
- 102000004196 processed proteins & peptides Human genes 0.000 description 2
- 238000000746 purification Methods 0.000 description 2
- 239000011541 reaction mixture Substances 0.000 description 2
- URGAHOPLAPQHLN-UHFFFAOYSA-N sodium aluminosilicate Chemical compound [Na+].[Al+3].[O-][Si]([O-])=O.[O-][Si]([O-])=O URGAHOPLAPQHLN-UHFFFAOYSA-N 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- YNJBWRMUSHSURL-UHFFFAOYSA-N trichloroacetic acid Chemical compound OC(=O)C(Cl)(Cl)Cl YNJBWRMUSHSURL-UHFFFAOYSA-N 0.000 description 2
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 2
- 229960005486 vaccine Drugs 0.000 description 2
- QBFPMEATQNQMEH-UHFFFAOYSA-N (n,n-dimethylcarbamimidoyl)-dimethylazanium;n-oxido-1-pyridin-2-ylmethanimine Chemical compound CN(C)C(=N)[NH+](C)C.[O-]N=CC1=CC=CC=N1 QBFPMEATQNQMEH-UHFFFAOYSA-N 0.000 description 1
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 1
- JKMHFZQWWAIEOD-UHFFFAOYSA-N 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid Chemical compound OCC[NH+]1CCN(CCS([O-])(=O)=O)CC1 JKMHFZQWWAIEOD-UHFFFAOYSA-N 0.000 description 1
- VKIGAWAEXPTIOL-UHFFFAOYSA-N 2-hydroxyhexanenitrile Chemical compound CCCCC(O)C#N VKIGAWAEXPTIOL-UHFFFAOYSA-N 0.000 description 1
- SFYDWLYPIXHPML-UHFFFAOYSA-N 3-nitro-1-(2,4,6-trimethylphenyl)sulfonyl-1,2,4-triazole Chemical compound CC1=CC(C)=CC(C)=C1S(=O)(=O)N1N=C([N+]([O-])=O)N=C1 SFYDWLYPIXHPML-UHFFFAOYSA-N 0.000 description 1
- 102000002260 Alkaline Phosphatase Human genes 0.000 description 1
- 108020004774 Alkaline Phosphatase Proteins 0.000 description 1
- VHUUQVKOLVNVRT-UHFFFAOYSA-N Ammonium hydroxide Chemical compound [NH4+].[OH-] VHUUQVKOLVNVRT-UHFFFAOYSA-N 0.000 description 1
- 241000972773 Aulopiformes Species 0.000 description 1
- 241001367049 Autographa Species 0.000 description 1
- 244000063299 Bacillus subtilis Species 0.000 description 1
- 235000014469 Bacillus subtilis Nutrition 0.000 description 1
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 1
- VEXZGXHMUGYJMC-UHFFFAOYSA-M Chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 description 1
- 102000007644 Colony-Stimulating Factors Human genes 0.000 description 1
- 108010071942 Colony-Stimulating Factors Proteins 0.000 description 1
- 230000004568 DNA-binding Effects 0.000 description 1
- 108010067770 Endopeptidase K Proteins 0.000 description 1
- 241000701959 Escherichia virus Lambda Species 0.000 description 1
- 108060002716 Exonuclease Proteins 0.000 description 1
- 108700007698 Genetic Terminator Regions Proteins 0.000 description 1
- 239000007995 HEPES buffer Substances 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 101000959820 Homo sapiens Interferon alpha-1/13 Proteins 0.000 description 1
- 102000002265 Human Growth Hormone Human genes 0.000 description 1
- 108010000521 Human Growth Hormone Proteins 0.000 description 1
- 239000000854 Human Growth Hormone Substances 0.000 description 1
- 108090000144 Human Proteins Proteins 0.000 description 1
- 102000003839 Human Proteins Human genes 0.000 description 1
- 101150103227 IFN gene Proteins 0.000 description 1
- 108090001061 Insulin Proteins 0.000 description 1
- 102000004877 Insulin Human genes 0.000 description 1
- 108090000723 Insulin-Like Growth Factor I Proteins 0.000 description 1
- 102100034343 Integrase Human genes 0.000 description 1
- 102100040019 Interferon alpha-1/13 Human genes 0.000 description 1
- 102100026720 Interferon beta Human genes 0.000 description 1
- 102000008070 Interferon-gamma Human genes 0.000 description 1
- 108010074328 Interferon-gamma Proteins 0.000 description 1
- 102000015696 Interleukins Human genes 0.000 description 1
- 108010063738 Interleukins Proteins 0.000 description 1
- 108091026898 Leader sequence (mRNA) Proteins 0.000 description 1
- 102000003960 Ligases Human genes 0.000 description 1
- 108090000364 Ligases Proteins 0.000 description 1
- 102000008072 Lymphokines Human genes 0.000 description 1
- 108010074338 Lymphokines Proteins 0.000 description 1
- 241000204801 Muraenidae Species 0.000 description 1
- 239000000020 Nitrocellulose Substances 0.000 description 1
- 108091034117 Oligonucleotide Proteins 0.000 description 1
- 241000283973 Oryctolagus cuniculus Species 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 101710182846 Polyhedrin Proteins 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 108091034057 RNA (poly(A)) Proteins 0.000 description 1
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 description 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 1
- 241000235070 Saccharomyces Species 0.000 description 1
- 241000831652 Salinivibrio sharmensis Species 0.000 description 1
- 229920005654 Sephadex Polymers 0.000 description 1
- 239000012507 Sephadex™ Substances 0.000 description 1
- 238000012300 Sequence Analysis Methods 0.000 description 1
- 102000013275 Somatomedins Human genes 0.000 description 1
- 241000256251 Spodoptera frugiperda Species 0.000 description 1
- 108700026226 TATA Box Proteins 0.000 description 1
- 239000004098 Tetracycline Substances 0.000 description 1
- 206010054094 Tumour necrosis Diseases 0.000 description 1
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- FTAJFJHHNHPSBP-UHFFFAOYSA-N [N+](=O)([O-])C1=[C-]N=NN1 Chemical compound [N+](=O)([O-])C1=[C-]N=NN1 FTAJFJHHNHPSBP-UHFFFAOYSA-N 0.000 description 1
- 230000003187 abdominal effect Effects 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 210000000577 adipose tissue Anatomy 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- QGZKDVFQNNGYKY-UHFFFAOYSA-N ammonia Natural products N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 1
- 210000004381 amniotic fluid Anatomy 0.000 description 1
- 229960000723 ampicillin Drugs 0.000 description 1
- AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 108010005774 beta-Galactosidase Proteins 0.000 description 1
- 102000005936 beta-Galactosidase Human genes 0.000 description 1
- 230000001588 bifunctional effect Effects 0.000 description 1
- 239000001110 calcium chloride Substances 0.000 description 1
- 229910001628 calcium chloride Inorganic materials 0.000 description 1
- 239000004202 carbamide Substances 0.000 description 1
- 239000006143 cell culture medium Substances 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 239000012461 cellulose resin Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 230000002759 chromosomal effect Effects 0.000 description 1
- 239000013599 cloning vector Substances 0.000 description 1
- 229940047120 colony stimulating factors Drugs 0.000 description 1
- 238000004440 column chromatography Methods 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- RGWHQCVHVJXOKC-SHYZEUOFSA-J dCTP(4-) Chemical compound O=C1N=C(N)C=CN1[C@@H]1O[C@H](COP([O-])(=O)OP([O-])(=O)OP([O-])([O-])=O)[C@@H](O)C1 RGWHQCVHVJXOKC-SHYZEUOFSA-J 0.000 description 1
- 230000006735 deficit Effects 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000000502 dialysis Methods 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 208000035475 disorder Diseases 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000012869 ethanol precipitation Methods 0.000 description 1
- 210000003527 eukaryotic cell Anatomy 0.000 description 1
- 108010052305 exodeoxyribonuclease III Proteins 0.000 description 1
- 102000013165 exonuclease Human genes 0.000 description 1
- 108010092809 exonuclease Bal 31 Proteins 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 239000012091 fetal bovine serum Substances 0.000 description 1
- 239000012894 fetal calf serum Substances 0.000 description 1
- 238000007667 floating Methods 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 238000002523 gelfiltration Methods 0.000 description 1
- 238000012215 gene cloning Methods 0.000 description 1
- 238000012224 gene deletion Methods 0.000 description 1
- 208000007565 gingivitis Diseases 0.000 description 1
- 229960000789 guanidine hydrochloride Drugs 0.000 description 1
- PJJJBBJSCAKJQF-UHFFFAOYSA-N guanidinium chloride Chemical compound [Cl-].NC(N)=[NH2+] PJJJBBJSCAKJQF-UHFFFAOYSA-N 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 208000005252 hepatitis A Diseases 0.000 description 1
- 208000002672 hepatitis B Diseases 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 238000010348 incorporation Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 229960003971 influenza vaccine Drugs 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 229940125396 insulin Drugs 0.000 description 1
- 229960003130 interferon gamma Drugs 0.000 description 1
- 229940047122 interleukins Drugs 0.000 description 1
- 210000000265 leukocyte Anatomy 0.000 description 1
- 210000004324 lymphatic system Anatomy 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- AUHZEENZYGFFBQ-UHFFFAOYSA-N mesitylene Substances CC1=CC(C)=CC(C)=C1 AUHZEENZYGFFBQ-UHFFFAOYSA-N 0.000 description 1
- 125000001827 mesitylenyl group Chemical group [H]C1=C(C(*)=C(C([H])=C1C([H])([H])[H])C([H])([H])[H])C([H])([H])[H] 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000010369 molecular cloning Methods 0.000 description 1
- 229920001220 nitrocellulos Polymers 0.000 description 1
- 239000002777 nucleoside Substances 0.000 description 1
- 150000003833 nucleoside derivatives Chemical class 0.000 description 1
- 230000007170 pathology Effects 0.000 description 1
- 239000008188 pellet Substances 0.000 description 1
- 239000008363 phosphate buffer Substances 0.000 description 1
- 229920002401 polyacrylamide Polymers 0.000 description 1
- 229920005990 polystyrene resin Polymers 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 210000001995 reticulocyte Anatomy 0.000 description 1
- 235000019515 salmon Nutrition 0.000 description 1
- 239000012047 saturated solution Substances 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 239000002356 single layer Substances 0.000 description 1
- 230000005783 single-strand break Effects 0.000 description 1
- 238000002741 site-directed mutagenesis Methods 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 229910000029 sodium carbonate Inorganic materials 0.000 description 1
- 238000010532 solid phase synthesis reaction Methods 0.000 description 1
- 238000007711 solidification Methods 0.000 description 1
- 230000008023 solidification Effects 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 239000007858 starting material Substances 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 125000000472 sulfonyl group Chemical group *S(*)(=O)=O 0.000 description 1
- YBRBMKDOPFTVDT-UHFFFAOYSA-N tert-butylamine Chemical compound CC(C)(C)N YBRBMKDOPFTVDT-UHFFFAOYSA-N 0.000 description 1
- 229960002180 tetracycline Drugs 0.000 description 1
- 229930101283 tetracycline Natural products 0.000 description 1
- 235000019364 tetracycline Nutrition 0.000 description 1
- 150000003522 tetracyclines Chemical class 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 238000003151 transfection method Methods 0.000 description 1
- 238000000844 transformation Methods 0.000 description 1
- AVBGNFCMKJOFIN-UHFFFAOYSA-N triethylammonium acetate Chemical compound CC(O)=O.CCN(CC)CC AVBGNFCMKJOFIN-UHFFFAOYSA-N 0.000 description 1
- 241000701447 unidentified baculovirus Species 0.000 description 1
- 108700026220 vif Genes Proteins 0.000 description 1
- 230000003612 virological effect Effects 0.000 description 1
- 239000002023 wood Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
- C12N15/86—Viral vectors
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/52—Cytokines; Lymphokines; Interferons
- C07K14/555—Interferons [IFN]
- C07K14/56—IFN-alpha
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
- C12N2710/00011—Details
- C12N2710/14011—Baculoviridae
- C12N2710/14111—Nucleopolyhedrovirus, e.g. autographa californica nucleopolyhedrovirus
- C12N2710/14141—Use of virus, viral particle or viral elements as a vector
- C12N2710/14143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Toxicology (AREA)
- Gastroenterology & Hepatology (AREA)
- Virology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Medicinal Chemistry (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Diaphragms For Electromechanical Transducers (AREA)
- Video Image Reproduction Devices For Color Tv Systems (AREA)
- Saccharide Compounds (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
- Transition And Organic Metals Composition Catalysts For Addition Polymerization (AREA)
- Casting Or Compression Moulding Of Plastics Or The Like (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP59128215A JPS619288A (ja) | 1984-06-21 | 1984-06-21 | ペプチド類の製法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| HUT38969A HUT38969A (en) | 1986-07-28 |
| HU202581B true HU202581B (en) | 1991-03-28 |
Family
ID=14979338
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| HU852434A HU202581B (en) | 1984-06-21 | 1985-06-20 | Process for producing valuable protein materials with utilizing recombinant dns of bomby mori polyhedrosis virus |
Country Status (17)
| Country | Link |
|---|---|
| US (1) | US5118616A (https=) |
| EP (1) | EP0175852B1 (https=) |
| JP (1) | JPS619288A (https=) |
| KR (1) | KR930000273B1 (https=) |
| AT (1) | ATE71655T1 (https=) |
| AU (1) | AU588236B2 (https=) |
| BG (1) | BG60254B1 (https=) |
| CA (1) | CA1284119C (https=) |
| DE (1) | DE3585194D1 (https=) |
| DK (1) | DK173054B1 (https=) |
| FI (1) | FI89184C (https=) |
| HU (1) | HU202581B (https=) |
| IE (1) | IE59420B1 (https=) |
| IL (1) | IL75584A (https=) |
| NO (1) | NO175215C (https=) |
| PH (1) | PH21679A (https=) |
| PL (1) | PL152355B1 (https=) |
Families Citing this family (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| IL80529A0 (en) * | 1985-11-14 | 1987-02-27 | Daiichi Seiyaku Co | Method of producing peptides |
| JPS63167797A (ja) * | 1985-12-18 | 1988-07-11 | マイクロジエネシス,インコ−ポレイテイド | 選択された昆虫宿主細胞中で選択されたポリペプチドを製造する方法 |
| US5071748A (en) * | 1986-09-09 | 1991-12-10 | Genetics Institute, Inc. | Mixed baculovirus compositions and uses thereof |
| JPS6474990A (en) * | 1987-09-17 | 1989-03-20 | Susumu Maeda | Vector, recombinant dna, virus and production of protein |
| US5145775A (en) * | 1989-02-28 | 1992-09-08 | Research Association For Biotechnology Of Agricultural Chemicals | Polyhedrin gene and genetic engineering thereof |
| CA2019803C (en) * | 1989-06-29 | 2000-04-25 | Akira Yanai | Feline interferon and process for production thereof |
| JP2525054B2 (ja) * | 1989-08-01 | 1996-08-14 | 株式会社トクヤマ | 成人t細胞白血病ウィルス感染診断薬 |
| EP0533469A1 (en) * | 1991-09-18 | 1993-03-24 | Hong Kong Tech Company Limited | Expression vector and silkworm larvae transformant containing the same |
| JPH05227967A (ja) * | 1992-02-17 | 1993-09-07 | Katakura Kogyo Kk | カイコからの有用タンパク質の製造方法 |
| US6575013B2 (en) * | 2001-02-26 | 2003-06-10 | Lucent Technologies Inc. | Electronic odor sensor |
| EP1613909B1 (en) | 2003-03-18 | 2013-03-06 | Air Products And Chemicals, Inc. | Integrated multiple-loop refrigeration process for gas liquefaction |
| JP5071740B2 (ja) * | 2006-05-19 | 2012-11-14 | 国立大学法人山口大学 | チョウ目昆虫用人工飼料及びその製造方法、チョウ目昆虫及びその製造方法、並びに生体物質 |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4745051A (en) * | 1983-05-27 | 1988-05-17 | The Texas A&M University System | Method for producing a recombinant baculovirus expression vector |
| EP0127839B1 (en) * | 1983-05-27 | 1992-07-15 | THE TEXAS A&M UNIVERSITY SYSTEM | Method for producing a recombinant baculovirus expression vector |
| ZA848495B (en) * | 1984-01-31 | 1985-09-25 | Idaho Res Found | Production of polypeptides in insect cells |
-
1984
- 1984-06-21 JP JP59128215A patent/JPS619288A/ja active Granted
-
1985
- 1985-06-11 FI FI852322A patent/FI89184C/fi not_active IP Right Cessation
- 1985-06-17 NO NO852433A patent/NO175215C/no unknown
- 1985-06-19 PH PH32421A patent/PH21679A/en unknown
- 1985-06-20 HU HU852434A patent/HU202581B/hu unknown
- 1985-06-20 IL IL7558485A patent/IL75584A/en not_active IP Right Cessation
- 1985-06-20 PL PL1985254100A patent/PL152355B1/pl unknown
- 1985-06-21 EP EP85107711A patent/EP0175852B1/en not_active Expired - Lifetime
- 1985-06-21 KR KR1019850004412A patent/KR930000273B1/ko not_active Expired - Lifetime
- 1985-06-21 IE IE154885A patent/IE59420B1/en not_active IP Right Cessation
- 1985-06-21 AT AT85107711T patent/ATE71655T1/de not_active IP Right Cessation
- 1985-06-21 AU AU43943/85A patent/AU588236B2/en not_active Expired
- 1985-06-21 BG BG070778A patent/BG60254B1/bg unknown
- 1985-06-21 DE DE8585107711T patent/DE3585194D1/de not_active Expired - Lifetime
- 1985-06-21 DK DK198502823A patent/DK173054B1/da not_active IP Right Cessation
- 1985-06-21 CA CA000484915A patent/CA1284119C/en not_active Expired - Lifetime
-
1991
- 1991-04-12 US US07/684,486 patent/US5118616A/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| PL254100A1 (en) | 1986-08-12 |
| AU588236B2 (en) | 1989-09-14 |
| CA1284119C (en) | 1991-05-14 |
| DK173054B1 (da) | 1999-12-06 |
| BG60254B2 (en) | 1994-03-24 |
| KR860000383A (ko) | 1986-01-28 |
| EP0175852A3 (en) | 1987-09-16 |
| NO175215B (https=) | 1994-06-06 |
| NO852433L (no) | 1985-12-23 |
| ATE71655T1 (de) | 1992-02-15 |
| FI852322L (fi) | 1985-12-22 |
| PL152355B1 (en) | 1990-12-31 |
| KR930000273B1 (ko) | 1993-01-14 |
| FI89184B (fi) | 1993-05-14 |
| EP0175852A2 (en) | 1986-04-02 |
| FI89184C (fi) | 1993-08-25 |
| DK282385D0 (da) | 1985-06-21 |
| IE59420B1 (en) | 1994-02-23 |
| HUT38969A (en) | 1986-07-28 |
| EP0175852B1 (en) | 1992-01-15 |
| JPH0573389B2 (https=) | 1993-10-14 |
| IL75584A0 (en) | 1985-10-31 |
| PH21679A (en) | 1988-01-13 |
| NO175215C (no) | 1994-09-14 |
| DK282385A (da) | 1985-12-22 |
| BG60254B1 (bg) | 1994-03-24 |
| IE851548L (en) | 1985-12-21 |
| IL75584A (en) | 1994-05-30 |
| FI852322A0 (fi) | 1985-06-11 |
| AU4394385A (en) | 1986-01-02 |
| DE3585194D1 (de) | 1992-02-27 |
| JPS619288A (ja) | 1986-01-16 |
| US5118616A (en) | 1992-06-02 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| FI88175B (fi) | Rekombinant-dna-molekyler och foerfaranden foer framstaellning av polypeptider liknande humant -interferon | |
| Armour et al. | Helper component for aphid transmission encoded by region II of cauliflower mosaic virus DNA | |
| EP0429483B1 (en) | Potyvirus coat protein genes and plants transformed therewith | |
| KR970000595B1 (ko) | 재조합 종두 비루스 | |
| HU202581B (en) | Process for producing valuable protein materials with utilizing recombinant dns of bomby mori polyhedrosis virus | |
| GB2110694A (en) | Stabilizing and selecting cells | |
| KR950001992B1 (ko) | 배양된 세포내에서 바쿨로바이러스 벡터를 이용한 펩타이드의 제조방법 | |
| Horiuchi et al. | High-level expression of the human-α-interferon gene through the use of an improved baculovirus vector in the silkworm, Bombyx mori | |
| KR970002670B1 (ko) | 고양이 인터페론 및 이의 제조방법 | |
| JPH0365191A (ja) | スフェロイジン単離dnaおよび組み換え昆虫ポックスウィルス発現ベクター | |
| WO1993025666A1 (en) | Recombinant entomopoxvirus | |
| EP0228036A2 (en) | Method for producing selected polypeptides in virally infected insect cells and polypeptides isolated therefrom | |
| RU2031122C1 (ru) | Способ встраивания гетерологичной днк в геном аденовируса celo и рекомбинантный аденовирусный вектор celo/рuc19 | |
| KR940001265B1 (ko) | 프로모터, 그를 함유하는 플라스미드 및 재조합 아비폭스비루스 | |
| US5145775A (en) | Polyhedrin gene and genetic engineering thereof | |
| CN85104701A (zh) | 生产有用物质的方法 | |
| JPH0573393B2 (https=) | ||
| KR100325394B1 (ko) | 신규한 진핵세포용 유전자 클로닝 전달운반체와 재조합 바이러스 운반체 및 이들을 이용한 재조합 단백질 제조방법 | |
| AU668734C (en) | Recombinant entomopoxvirus | |
| Davis et al. | Genes differentially expressed during pupal-adult neurogenesis in Manduca sexta | |
| Mesnard et al. | Characterization of different electrophoretic forms of cauliflower mosaic virus virions (strain Cabb-S) | |
| CN1062001A (zh) | 利用家蚕高效表达天花粉蛋白及其他有用蛋白的方法 | |
| HU204086B (en) | Process for producing non-human mammal animal interferons, dna sequemces coding form them and pharmaceutical compositions comprising sand interferons | |
| AU4297393A (en) | Recombinant entomopoxvirus | |
| KR19980072608A (ko) | 곤충세포를 이용한 인간 트롬보포이에틴의 제조방법 |