HRP20200717T1 - Postupak proizvodnje enzimskog koktela iz gljivičnog mošta - Google Patents
Postupak proizvodnje enzimskog koktela iz gljivičnog mošta Download PDFInfo
- Publication number
- HRP20200717T1 HRP20200717T1 HRP20200717TT HRP20200717T HRP20200717T1 HR P20200717 T1 HRP20200717 T1 HR P20200717T1 HR P20200717T T HRP20200717T T HR P20200717TT HR P20200717 T HRP20200717 T HR P20200717T HR P20200717 T1 HRP20200717 T1 HR P20200717T1
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- Croatia
- Prior art keywords
- separated
- enzyme
- cooling step
- liquid
- previous
- Prior art date
Links
- 238000004519 manufacturing process Methods 0.000 title claims 8
- 230000002255 enzymatic effect Effects 0.000 title 1
- 230000002538 fungal effect Effects 0.000 title 1
- 108090000790 Enzymes Proteins 0.000 claims 18
- 102000004190 Enzymes Human genes 0.000 claims 18
- 238000000034 method Methods 0.000 claims 15
- 238000001816 cooling Methods 0.000 claims 10
- 239000007788 liquid Substances 0.000 claims 7
- 244000005700 microbiome Species 0.000 claims 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims 2
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 claims 2
- 102000006995 beta-Glucosidase Human genes 0.000 claims 2
- 108010047754 beta-Glucosidase Proteins 0.000 claims 2
- 108010038658 exo-1,4-beta-D-xylosidase Proteins 0.000 claims 2
- 102000005575 Cellulases Human genes 0.000 claims 1
- 108010084185 Cellulases Proteins 0.000 claims 1
- 241000223259 Trichoderma Species 0.000 claims 1
- 241000499912 Trichoderma reesei Species 0.000 claims 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims 1
- 229910002092 carbon dioxide Inorganic materials 0.000 claims 1
- 239000001569 carbon dioxide Substances 0.000 claims 1
- 238000005119 centrifugation Methods 0.000 claims 1
- 230000001461 cytolytic effect Effects 0.000 claims 1
- 238000001914 filtration Methods 0.000 claims 1
- 239000007789 gas Substances 0.000 claims 1
- 108010002430 hemicellulase Proteins 0.000 claims 1
- 238000001471 micro-filtration Methods 0.000 claims 1
- 230000007935 neutral effect Effects 0.000 claims 1
- 229910052757 nitrogen Inorganic materials 0.000 claims 1
- 239000001301 oxygen Substances 0.000 claims 1
- 229910052760 oxygen Inorganic materials 0.000 claims 1
- 238000001556 precipitation Methods 0.000 claims 1
- 238000003825 pressing Methods 0.000 claims 1
- 238000000926 separation method Methods 0.000 claims 1
- 239000007787 solid Substances 0.000 claims 1
- 238000000108 ultra-filtration Methods 0.000 claims 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/06—Lysis of microorganisms
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
- C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
- C12N9/2445—Beta-glucosidase (3.2.1.21)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2477—Hemicellulases not provided in a preceding group
- C12N9/248—Xylanases
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01021—Beta-glucosidase (3.2.1.21)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01037—Xylan 1,4-beta-xylosidase (3.2.1.37)
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Mycology (AREA)
- Botany (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Claims (15)
1. Postupak za povećanje sadržaja β-glukozidaze i/ili β-ksilozidaze enzimskog koktela iz celulolitičkog mikroorganizma koji proizvodi celulaze i/ili hemi-celulaze, naznačen time što sadrži:
- korak za proizvodnju enzima s dobivenim medijem koji sadrži enzime i mošt mikroorganizma, navedeni mošt se odvaja, ili ne odvaja, iz tekućine koja sadrži navedene enzime,
- korak hlađenja navedenog mošta na temperaturu koja iznosi između 4 i 20°C, što je temperatura ispod temperature koraka proizvodnje enzima, tijekom vremena koje iznosi između 12 i 90 sati tako da:
- je koncentracija tekućine β-glukozidaze ili β-ksilozidaze koja potječe iz koraka hlađenja veća od koncentracije tekućine koja potječe iz koraka proizvodnje enzima,
i/ili
- omjer volumen krutine / ukupni volumen je manji od navedenog omjera za korak proizvodnje enzima,
- i enzimski koktel se dobije na kraju koraka hlađenja.
2. Postupak prema patentnom zahtjevu 1 naznačen time što trajanje koraka hlađenja iznosi između 24 i 72 sata.
3. Postupak prema bilo kojem od prethodnih patentnih zahtjeva naznačen time što se, na kraju koraka proizvodnje enzima, mošt odvaja od tekućine.
4. Postupak prema prethodnom patentnom zahtjevu naznačen time što se navedeni odvojeni mošt razrjeđuje vodom.
5. Postupak prema patentnom zahtjevu 1 ili 2 naznačen time što se mošt, na kraju koraka proizvodnje enzima, ne odvaja od tekućine.
6. Postupak prema jednom od prethodnih zahtjeva naznačen time što se, nakon koraka hlađenja, enzimski koktel koji potječe iz mošta odvaja i dobije se preostali mošt.
7. Postupak prema jednom od patentnih zahtjeva 3 ili 4 i 6 naznačen time što se odvajanje provodi pomoću barem jednog od centrifugiranja ili filtracije / prešanja ili mikrofiltracije, kojima proizvoljno prethodi taloženje.
8. Postupak prema patentnom zahtjevu 6 naznačen time što se enzimi odijeljene tekućine mogu koncentrirati, na primjer ultrafiltracijom.
9. Postupak prema jednom od prethodnih zahtjeva naznačen time što se korak hlađenja provodi pri pH koji se nalazi između 3,5 i 5,5.
10. Postupak prema jednom od prethodnih zahtjeva naznačen time što se korak hlađenja provodi u atmosferi osiromašenoj kisikom, poželjno u anaerobnoj atmosferi.
11. Postupak prema prethodnom patentnom zahtjevu naznačen time što se ubrizgava neutralni plin, na primjer ugljikov dioksid ili dušik.
12. Postupak prema jednom od prethodnih zahtjeva naznačen time što mikroorganizam pripada rodu Trichoderma, naročito Trichoderma reesei, a poželjno je soj CL847.
13. Postupak prema jednom od prethodnih zahtjeva naznačen time što se navedeni preostali mošt podvrgava koraku hlađenja i enzimski koktel, odvojen ili ne odvojen od preostalog mošta, dobiva se na kraju koraka hlađenja i poželjno navedeni enzimski koktel se odvaja od preostalog mošta iz navedenog koraka hlađenja.
14. Postupak prema jednom od prethodnih zahtjeva naznačen time što se miješaju enzimski kokteli koji potječu iz mošta.
15. Postupak prema jednom od prethodnih zahtjeva naznačen time što je najmanje jedan enzimski koktel pomiješan s odvojenom tekućinom koja sadrži enzime i koja potječe iz koraka proizvodnje enzima.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FR1457358A FR3024463B1 (fr) | 2014-07-30 | 2014-07-30 | Procede de production d'un cocktail enzymatique a partir de mout de champignon |
| PCT/EP2015/067139 WO2016016182A1 (fr) | 2014-07-30 | 2015-07-27 | Procede de production d'un cocktail enzymatique a partir de moût de champignon |
| EP15742261.9A EP3174979B8 (fr) | 2014-07-30 | 2015-07-27 | Procede de production d'un cocktail enzymatique a partir de moût de champignon |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| HRP20200717T1 true HRP20200717T1 (hr) | 2020-07-24 |
Family
ID=51787093
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| HRP20200717TT HRP20200717T1 (hr) | 2014-07-30 | 2020-05-05 | Postupak proizvodnje enzimskog koktela iz gljivičnog mošta |
Country Status (16)
| Country | Link |
|---|---|
| US (1) | US10214734B2 (hr) |
| EP (1) | EP3174979B8 (hr) |
| CN (1) | CN107075448B (hr) |
| BR (1) | BR112017001669B8 (hr) |
| CA (1) | CA2956372C (hr) |
| DK (1) | DK3174979T3 (hr) |
| ES (1) | ES2784253T3 (hr) |
| FR (1) | FR3024463B1 (hr) |
| HR (1) | HRP20200717T1 (hr) |
| HU (1) | HUE050216T2 (hr) |
| LT (1) | LT3174979T (hr) |
| PL (1) | PL3174979T3 (hr) |
| PT (1) | PT3174979T (hr) |
| RS (1) | RS60220B1 (hr) |
| SI (1) | SI3174979T1 (hr) |
| WO (1) | WO2016016182A1 (hr) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR3054141B1 (fr) * | 2016-07-22 | 2018-07-13 | Institut National De La Recherche Agronomique | Separation des enzymes issues de trichoderma reesei par filtre presse et filtration tangentielle sur membrane ceramique. |
| FR3071852A1 (fr) * | 2017-09-29 | 2019-04-05 | IFP Energies Nouvelles | Souche de trichoderma reesei hyperproductrice et presentant une activite beta-glucosidase amelioree |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4628029A (en) * | 1983-08-25 | 1986-12-09 | Parsons & Whittemore, Inc. | Method for the conversion of a cellulosic substrate to glucose using Microbispora bispora, strain Rutgers P&W |
| ES2810026T3 (es) * | 2004-03-25 | 2021-03-08 | Novozymes Inc | Métodos para la degradación o la conversión de polisacáridos de la pared celular vegetal |
-
2014
- 2014-07-30 FR FR1457358A patent/FR3024463B1/fr not_active Expired - Fee Related
-
2015
- 2015-07-27 ES ES15742261T patent/ES2784253T3/es active Active
- 2015-07-27 EP EP15742261.9A patent/EP3174979B8/fr active Active
- 2015-07-27 SI SI201531196T patent/SI3174979T1/sl unknown
- 2015-07-27 BR BR112017001669A patent/BR112017001669B8/pt active IP Right Grant
- 2015-07-27 PL PL15742261T patent/PL3174979T3/pl unknown
- 2015-07-27 CA CA2956372A patent/CA2956372C/fr active Active
- 2015-07-27 CN CN201580040436.6A patent/CN107075448B/zh active Active
- 2015-07-27 PT PT157422619T patent/PT3174979T/pt unknown
- 2015-07-27 DK DK15742261.9T patent/DK3174979T3/da active
- 2015-07-27 HU HUE15742261A patent/HUE050216T2/hu unknown
- 2015-07-27 RS RS20200521A patent/RS60220B1/sr unknown
- 2015-07-27 LT LTEP15742261.9T patent/LT3174979T/lt unknown
- 2015-07-27 US US15/500,299 patent/US10214734B2/en active Active
- 2015-07-27 WO PCT/EP2015/067139 patent/WO2016016182A1/fr active Application Filing
-
2020
- 2020-05-05 HR HRP20200717TT patent/HRP20200717T1/hr unknown
Also Published As
| Publication number | Publication date |
|---|---|
| FR3024463A1 (fr) | 2016-02-05 |
| BR112017001669B8 (pt) | 2024-02-06 |
| EP3174979A1 (fr) | 2017-06-07 |
| RS60220B1 (sr) | 2020-06-30 |
| WO2016016182A1 (fr) | 2016-02-04 |
| EP3174979B1 (fr) | 2020-02-19 |
| CA2956372C (fr) | 2023-10-03 |
| PL3174979T3 (pl) | 2020-11-02 |
| CA2956372A1 (fr) | 2016-02-04 |
| PT3174979T (pt) | 2020-04-08 |
| US20170211051A1 (en) | 2017-07-27 |
| US10214734B2 (en) | 2019-02-26 |
| FR3024463B1 (fr) | 2018-04-06 |
| CN107075448A (zh) | 2017-08-18 |
| DK3174979T3 (da) | 2020-05-11 |
| LT3174979T (lt) | 2020-05-25 |
| HUE050216T2 (hu) | 2020-11-30 |
| ES2784253T3 (es) | 2020-09-23 |
| BR112017001669A2 (pt) | 2018-02-14 |
| BR112017001669B1 (pt) | 2023-04-18 |
| EP3174979B8 (fr) | 2020-04-01 |
| CN107075448B (zh) | 2021-06-11 |
| SI3174979T1 (sl) | 2020-08-31 |
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