HRP20200420T1 - Poboljšana alfa-v beta-8 protutijela - Google Patents
Poboljšana alfa-v beta-8 protutijela Download PDFInfo
- Publication number
- HRP20200420T1 HRP20200420T1 HRP20200420TT HRP20200420T HRP20200420T1 HR P20200420 T1 HRP20200420 T1 HR P20200420T1 HR P20200420T T HRP20200420T T HR P20200420TT HR P20200420 T HRP20200420 T HR P20200420T HR P20200420 T1 HRP20200420 T1 HR P20200420T1
- Authority
- HR
- Croatia
- Prior art keywords
- antibody
- main
- glycosylated
- amino acid
- antibodies
- Prior art date
Links
- 238000000034 method Methods 0.000 claims 5
- 150000001413 amino acids Chemical class 0.000 claims 3
- 230000013595 glycosylation Effects 0.000 claims 3
- 238000006206 glycosylation reaction Methods 0.000 claims 3
- 102100035361 Cerebellar degeneration-related protein 2 Human genes 0.000 claims 2
- 101000737796 Homo sapiens Cerebellar degeneration-related protein 2 Proteins 0.000 claims 2
- 108090001012 Transforming Growth Factor beta Proteins 0.000 claims 2
- 230000007423 decrease Effects 0.000 claims 2
- 238000004519 manufacturing process Methods 0.000 claims 2
- 239000000126 substance Substances 0.000 claims 2
- 125000003275 alpha amino acid group Chemical group 0.000 claims 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
- C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
- C07K16/2839—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the integrin superfamily
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/30—Immunoglobulins specific features characterized by aspects of specificity or valency
- C07K2317/34—Identification of a linear epitope shorter than 20 amino acid residues or of a conformational epitope defined by amino acid residues
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/40—Immunoglobulins specific features characterized by post-translational modification
- C07K2317/41—Glycosylation, sialylation, or fucosylation
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
- C07K2317/55—Fab or Fab'
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
- C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
- C07K2317/565—Complementarity determining region [CDR]
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/70—Immunoglobulins specific features characterized by effect upon binding to a cell or to an antigen
- C07K2317/76—Antagonist effect on antigen, e.g. neutralization or inhibition of binding
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Immunology (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Life Sciences & Earth Sciences (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Claims (5)
1. Postupak za proizvodnju rekombinantnog protutijela koje inhibira otpuštanje aktivnog, zrelog TGFβ peptida, koji obuhvaća:
identificiranje protutijela koje veže epitop u glavnoj i/ili hibridnim domenama β8;
rekombinantno modificiranje CDR2 sekvence teškog lanca za uvođenje mjesta glikozilacije; i
eksprimiranje protutijela, pri čemu, nakon vezanja na β8, te protutijelo uzrokuje konformacijsku promjenu koja smanjuje kut između glavne i hibridnih domena β8 u usporedbi s β8 koja nije bila u kontaktu s antitijelom.
2. Postupak za proizvodnju glikoziliranog protutijela koje inhibira otpuštanje aktivnog, zrelog TGFβ peptida, koji obuhvaća:
identificiranje protutijela koje veže epitop u glavnoj i/ili hibridnim domenama β8, i koje uključuje aminokiselinu koja se može glikozilirati u CDR2 sekvencu teškog lanca;
eksprimiranje protutijela; i
kemijsko glikoziliranje aminokiseline, pri čemu, nakon vezanja na β8, protutijelo uzrokuje konformacijsku promjenu koja smanjuje kut između glavne i hibridnih domena β8 u usporedbi s β8 koja je bila u kontaktu s protutijelom bez kemijske glikozilacije.
3. Postupak prema patentnom zahtjevu 1 ili 2, naznačen time što rekombinantno ili glikozilirano protutijelo veže epitop koji sadrži SEQ ID NO: 16.
4. Postupak prema bilo kojem od patentnih zahtjeva 1-3, naznačen time što, nakon vezanja β8, rekombinantno ili glikozilirano protutijelo smanjuje kut između glavne i hibridnih domena β8 za najmanje 5° u odnosu na β8 koja nije bila u kontaktu s protutijelom.
5. Postupak prema bilo kojem od patentnih zahtjeva 1-4, naznačen time što aminokiselina koja je glikozilirana odgovara položaju aminokiseline 10 u bilo kojoj od SEQ ID NO: 1-6.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201462013114P | 2014-06-17 | 2014-06-17 | |
| EP15809699.0A EP3157561B1 (en) | 2014-06-17 | 2015-06-17 | Improved alpha-v beta-8 antibodies |
| PCT/US2015/036284 WO2015195835A2 (en) | 2014-06-17 | 2015-06-17 | Improved alpha-v beta-8 antibodies |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| HRP20200420T1 true HRP20200420T1 (hr) | 2020-06-26 |
Family
ID=54936235
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| HRP20200420TT HRP20200420T1 (hr) | 2014-06-17 | 2020-03-16 | Poboljšana alfa-v beta-8 protutijela |
Country Status (16)
| Country | Link |
|---|---|
| US (2) | US20170129955A1 (hr) |
| EP (1) | EP3157561B1 (hr) |
| JP (2) | JP2017522289A (hr) |
| CN (1) | CN107405396A (hr) |
| CY (1) | CY1123219T1 (hr) |
| DK (1) | DK3157561T3 (hr) |
| ES (1) | ES2779412T3 (hr) |
| HR (1) | HRP20200420T1 (hr) |
| HU (1) | HUE048663T2 (hr) |
| LT (1) | LT3157561T (hr) |
| ME (1) | ME03678B (hr) |
| PL (1) | PL3157561T3 (hr) |
| PT (1) | PT3157561T (hr) |
| RS (1) | RS60061B1 (hr) |
| SI (1) | SI3157561T1 (hr) |
| WO (1) | WO2015195835A2 (hr) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3847194A1 (en) * | 2018-09-07 | 2021-07-14 | Pfizer Inc. | Anti-avb8 antibodies and compositions and uses thereof |
| TW202140554A (zh) | 2020-01-27 | 2021-11-01 | 英商梅迪繆思有限公司 | 用於治療腎臟疾病之抗αｖβ８整聯蛋白抗體 |
| CN117126282B (zh) * | 2023-10-26 | 2024-01-12 | 迈威(上海)生物科技股份有限公司 | 抗体及其在制备阻断αvβ8与Latent TGF-β的结合的药物中的应用 |
Family Cites Families (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2413974A1 (fr) | 1978-01-06 | 1979-08-03 | David Bernard | Sechoir pour feuilles imprimees par serigraphie |
| US4816567A (en) | 1983-04-08 | 1989-03-28 | Genentech, Inc. | Recombinant immunoglobin preparations |
| US4676980A (en) | 1985-09-23 | 1987-06-30 | The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services | Target specific cross-linked heteroantibodies |
| US4946778A (en) | 1987-09-21 | 1990-08-07 | Genex Corporation | Single polypeptide chain binding molecules |
| GB8823869D0 (en) | 1988-10-12 | 1988-11-16 | Medical Res Council | Production of antibodies |
| ES2096590T3 (es) | 1989-06-29 | 1997-03-16 | Medarex Inc | Reactivos biespecificos para la terapia del sida. |
| ATE204902T1 (de) | 1990-06-29 | 2001-09-15 | Large Scale Biology Corp | Melaninproduktion durch transformierte mikroorganismen |
| US5633425A (en) | 1990-08-29 | 1997-05-27 | Genpharm International, Inc. | Transgenic non-human animals capable of producing heterologous antibodies |
| US5661016A (en) | 1990-08-29 | 1997-08-26 | Genpharm International Inc. | Transgenic non-human animals capable of producing heterologous antibodies of various isotypes |
| US5625126A (en) | 1990-08-29 | 1997-04-29 | Genpharm International, Inc. | Transgenic non-human animals for producing heterologous antibodies |
| EP0546073B1 (en) | 1990-08-29 | 1997-09-10 | GenPharm International, Inc. | production and use of transgenic non-human animals capable of producing heterologous antibodies |
| US5545806A (en) | 1990-08-29 | 1996-08-13 | Genpharm International, Inc. | Ransgenic non-human animals for producing heterologous antibodies |
| WO1993008829A1 (en) | 1991-11-04 | 1993-05-13 | The Regents Of The University Of California | Compositions that mediate killing of hiv-infected cells |
| US5714350A (en) | 1992-03-09 | 1998-02-03 | Protein Design Labs, Inc. | Increasing antibody affinity by altering glycosylation in the immunoglobulin variable region |
| US7163681B2 (en) * | 2000-08-07 | 2007-01-16 | Centocor, Inc. | Anti-integrin antibodies, compositions, methods and uses |
| US20050025763A1 (en) * | 2003-05-08 | 2005-02-03 | Protein Design Laboratories, Inc. | Therapeutic use of anti-CS1 antibodies |
| SI2567976T1 (sl) * | 2005-03-23 | 2017-11-30 | Genmab A/S | Protitelesa usmerjena proti cd38 za zdravljenje multiplega mieloma |
| CA2660592C (en) * | 2006-05-26 | 2016-07-12 | Macrogenics, Inc. | Humanized fc.gamma.riib-specific antibodies and methods of use thereof |
| CN105315370A (zh) * | 2010-02-18 | 2016-02-10 | 加利福尼亚大学董事会 | 整合素αVβ8中和抗体 |
| WO2013026004A2 (en) * | 2011-08-17 | 2013-02-21 | The Regents Of The University Of California | Antibodies that bind integrin alpha-v beta-8 |
-
2015
- 2015-06-17 EP EP15809699.0A patent/EP3157561B1/en active Active
- 2015-06-17 US US15/319,147 patent/US20170129955A1/en not_active Abandoned
- 2015-06-17 RS RS20200311A patent/RS60061B1/sr unknown
- 2015-06-17 JP JP2016573732A patent/JP2017522289A/ja active Pending
- 2015-06-17 SI SI201531139T patent/SI3157561T1/sl unknown
- 2015-06-17 WO PCT/US2015/036284 patent/WO2015195835A2/en active Application Filing
- 2015-06-17 DK DK15809699.0T patent/DK3157561T3/da active
- 2015-06-17 ME MEP-2020-57A patent/ME03678B/me unknown
- 2015-06-17 CN CN201580044081.8A patent/CN107405396A/zh active Pending
- 2015-06-17 PL PL15809699T patent/PL3157561T3/pl unknown
- 2015-06-17 PT PT158096990T patent/PT3157561T/pt unknown
- 2015-06-17 LT LTEP15809699.0T patent/LT3157561T/lt unknown
- 2015-06-17 ES ES15809699T patent/ES2779412T3/es active Active
- 2015-06-17 HU HUE15809699A patent/HUE048663T2/hu unknown
-
2020
- 2020-03-16 HR HRP20200420TT patent/HRP20200420T1/hr unknown
- 2020-03-18 CY CY20201100249T patent/CY1123219T1/el unknown
- 2020-03-24 US US16/828,553 patent/US20200277381A1/en not_active Abandoned
- 2020-07-20 JP JP2020123886A patent/JP2020183425A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| LT3157561T (lt) | 2020-07-10 |
| EP3157561A2 (en) | 2017-04-26 |
| JP2017522289A (ja) | 2017-08-10 |
| CN107405396A (zh) | 2017-11-28 |
| PT3157561T (pt) | 2020-03-25 |
| HUE048663T2 (hu) | 2020-07-28 |
| PL3157561T3 (pl) | 2020-06-29 |
| EP3157561B1 (en) | 2019-12-18 |
| DK3157561T3 (da) | 2020-03-23 |
| ES2779412T3 (es) | 2020-08-17 |
| SI3157561T1 (sl) | 2020-08-31 |
| RS60061B1 (sr) | 2020-04-30 |
| ME03678B (me) | 2020-10-20 |
| US20170129955A1 (en) | 2017-05-11 |
| WO2015195835A3 (en) | 2016-03-10 |
| WO2015195835A2 (en) | 2015-12-23 |
| JP2020183425A (ja) | 2020-11-12 |
| EP3157561A4 (en) | 2018-02-28 |
| CY1123219T1 (el) | 2021-10-29 |
| US20200277381A1 (en) | 2020-09-03 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| HRP20200420T1 (hr) | Poboljšana alfa-v beta-8 protutijela | |
| AU2018256487A1 (en) | Antibodies that bind to TL1A and their uses | |
| BR112015003938A8 (pt) | Métodos de produção de polipeptídeos, de produção de aglutinantes multiespecíficos, de seleção de aglutinantes multiespecíficos, de seleção de anticorpos biespecíficos e de determinação de combinação, anticorpo biespecífico, formulação farmcêutica e uso de anticorpo biespecífico | |
| MX2018013738A (es) | Anticuerpos que comprenden dominios constantes quimericos. | |
| EA201700111A1 (ru) | Полипептидные конструкции и их применение | |
| BR112015004022A2 (pt) | aminoácidos modificados compreendendo um grupo azido | |
| WO2013055058A3 (ko) | 항체결합 펩타이드-페리틴 융합단백질 및 이의 용도 | |
| MX357231B (es) | Polipeptidos de anticuerpos que antagonizan el ligando cd40 (cd40l). | |
| WO2014177459A3 (en) | Fc-receptor binding modified asymmetric antibodies and methods of use | |
| NZ701444A (en) | Antibodies to matrix metalloproteinase 9 | |
| EP3929217A3 (en) | Specific sites for modifying antibodies to make immunoconjugates | |
| JP2014012688A5 (hr) | ||
| BR112014001573B8 (pt) | Molécula fv ao antígeno multivalente | |
| WO2014124258A3 (en) | Specific sites for modifying antibodies to make immunoconjugates | |
| IN2015DN00552A (hr) | ||
| NZ630847A (en) | Anti-sema4d antibodies and epitopes | |
| MX353464B (es) | Antigenos tpn47 de treponema pallidum, inmunoreactivos, solubles. | |
| MY177098A (en) | Novel anti-human tslp receptor antibody | |
| WO2015006337A3 (en) | Compositions and methods for increasing protein half-life in a serum | |
| WO2014144911A3 (en) | Hybrid immunoglobulin containing non-peptidyl linkage | |
| JP2017510627A5 (hr) | ||
| UA117493C2 (uk) | Гібридна конструкція, що включає антигенсполучний фрагмент, специфічний до сироваткового альбуміну, й ефекторний компонент, та способи її одержання | |
| BR112018009851A2 (pt) | polipeptídios mutados de hev e uso dos mesmos para a contagem de anticorpos anti-hev | |
| MX2017007209A (es) | Prueba y metodo para determinar anticuerpos de produccion de citotoxicidad dependiente del complemento (cdc). | |
| WO2016138526A3 (en) | Single chain antibody domains for detection of anti-botulinum neurotoxin domains and methods of their use |