HRP20171624T1 - Biotehnološka proizvodnja hondroitina - Google Patents
Biotehnološka proizvodnja hondroitina Download PDFInfo
- Publication number
- HRP20171624T1 HRP20171624T1 HRP20171624TT HRP20171624T HRP20171624T1 HR P20171624 T1 HRP20171624 T1 HR P20171624T1 HR P20171624T T HRP20171624T T HR P20171624TT HR P20171624 T HRP20171624 T HR P20171624T HR P20171624 T1 HRP20171624 T1 HR P20171624T1
- Authority
- HR
- Croatia
- Prior art keywords
- recombinant microorganism
- escherichia coli
- chondroitin
- seq
- protein
- Prior art date
Links
- 229920002567 Chondroitin Polymers 0.000 title claims 7
- DLGJWSVWTWEWBJ-HGGSSLSASA-N chondroitin Chemical compound CC(O)=N[C@@H]1[C@H](O)O[C@H](CO)[C@H](O)[C@@H]1OC1[C@H](O)[C@H](O)C=C(C(O)=O)O1 DLGJWSVWTWEWBJ-HGGSSLSASA-N 0.000 title claims 7
- 238000013452 biotechnological production Methods 0.000 title claims 4
- 244000005700 microbiome Species 0.000 claims 17
- 241000588724 Escherichia coli Species 0.000 claims 12
- 108090000623 proteins and genes Proteins 0.000 claims 12
- 102000004169 proteins and genes Human genes 0.000 claims 6
- 238000000034 method Methods 0.000 claims 5
- 239000002773 nucleotide Substances 0.000 claims 5
- 125000003729 nucleotide group Chemical group 0.000 claims 5
- IAJILQKETJEXLJ-UHFFFAOYSA-N Galacturonsaeure Natural products O=CC(O)C(O)C(O)C(O)C(O)=O IAJILQKETJEXLJ-UHFFFAOYSA-N 0.000 claims 4
- 108010042889 Inulosucrase Proteins 0.000 claims 4
- MBLBDJOUHNCFQT-UHFFFAOYSA-N N-acetyl-D-galactosamine Natural products CC(=O)NC(C=O)C(O)C(O)C(O)CO MBLBDJOUHNCFQT-UHFFFAOYSA-N 0.000 claims 4
- 125000003275 alpha amino acid group Chemical group 0.000 claims 4
- AEMOLEFTQBMNLQ-WAXACMCWSA-N alpha-D-glucuronic acid Chemical compound O[C@H]1O[C@H](C(O)=O)[C@@H](O)[C@H](O)[C@H]1O AEMOLEFTQBMNLQ-WAXACMCWSA-N 0.000 claims 4
- 230000000694 effects Effects 0.000 claims 4
- 238000012217 deletion Methods 0.000 claims 3
- 230000037430 deletion Effects 0.000 claims 3
- 238000006467 substitution reaction Methods 0.000 claims 3
- 241000894006 Bacteria Species 0.000 claims 2
- 238000003780 insertion Methods 0.000 claims 2
- 230000037431 insertion Effects 0.000 claims 2
- 101001123526 Ambrosia artemisiifolia Pectate lyase 4 Proteins 0.000 claims 1
- 241000589876 Campylobacter Species 0.000 claims 1
- 108090000790 Enzymes Proteins 0.000 claims 1
- 102000004190 Enzymes Human genes 0.000 claims 1
- 241000588722 Escherichia Species 0.000 claims 1
- 241001464427 Gloeocapsa Species 0.000 claims 1
- 229920002683 Glycosaminoglycan Polymers 0.000 claims 1
- 241000606790 Haemophilus Species 0.000 claims 1
- OVRNDRQMDRJTHS-CBQIKETKSA-N N-Acetyl-D-Galactosamine Chemical compound CC(=O)N[C@H]1[C@@H](O)O[C@H](CO)[C@H](O)[C@@H]1O OVRNDRQMDRJTHS-CBQIKETKSA-N 0.000 claims 1
- 241000607598 Vibrio Species 0.000 claims 1
- 150000001413 amino acids Chemical class 0.000 claims 1
- 239000000427 antigen Substances 0.000 claims 1
- 102000036639 antigens Human genes 0.000 claims 1
- 108091007433 antigens Proteins 0.000 claims 1
- 230000000295 complement effect Effects 0.000 claims 1
- BJHIKXHVCXFQLS-UYFOZJQFSA-N fructose group Chemical group OCC(=O)[C@@H](O)[C@H](O)[C@H](O)CO BJHIKXHVCXFQLS-UYFOZJQFSA-N 0.000 claims 1
- 229930027917 kanamycin Natural products 0.000 claims 1
- SBUJHOSQTJFQJX-NOAMYHISSA-N kanamycin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N SBUJHOSQTJFQJX-NOAMYHISSA-N 0.000 claims 1
- 229960000318 kanamycin Drugs 0.000 claims 1
- 229930182823 kanamycin A Natural products 0.000 claims 1
- 238000009629 microbiological culture Methods 0.000 claims 1
- 238000000746 purification Methods 0.000 claims 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/006—Heteroglycans, i.e. polysaccharides having more than one sugar residue in the main chain in either alternating or less regular sequence; Gellans; Succinoglycans; Arabinogalactans; Tragacanth or gum tragacanth or traganth from Astragalus; Gum Karaya from Sterculia urens; Gum Ghatti from Anogeissus latifolia; Derivatives thereof
- C08B37/0063—Glycosaminoglycans or mucopolysaccharides, e.g. keratan sulfate; Derivatives thereof, e.g. fucoidan
- C08B37/0069—Chondroitin-4-sulfate, i.e. chondroitin sulfate A; Dermatan sulfate, i.e. chondroitin sulfate B or beta-heparin; Chondroitin-6-sulfate, i.e. chondroitin sulfate C; Derivatives thereof
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1048—Glycosyltransferases (2.4)
- C12N9/1051—Hexosyltransferases (2.4.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y204/00—Glycosyltransferases (2.4)
- C12Y204/01—Hexosyltransferases (2.4.1)
- C12Y204/01099—Sucrose:sucrose fructosyltransferase (2.4.1.99)
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Materials Engineering (AREA)
- Dermatology (AREA)
- Polymers & Plastics (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
- Materials For Medical Uses (AREA)
- Enzymes And Modification Thereof (AREA)
Claims (14)
1. Rekombinantni mikroorganizam koji proizvodi hondroitin, definiran kao nerazgranati glikozaminoglikan, kojega čine izmjenjujući ostaci d-glukuronske kiseline (GlcUA) i N-acetil-d-galaktozamina (GalNAc), vezani vezama β1-3 (GlcUA → GalNAc) i β1-4 (GalNAc → GlcUA), naznačen time što je navedeni mikroorganizam izveden iz mikroorganizma koji pripada skupini s antigenom K, gdje je u navedenom mikroorganizmu izvorno prisutan gen kfoE, koji kodira enzim odgovoran za dodavanje fruktoznih ostataka na nerazgranatu okosnica hondroitina, inaktiviran potpunom ili djelomičnom delecijom ili supstitucijom navedenog gena ili disrupcijom insercijom dodatnog nukleotidnog slijeda, te gdje navedeni gen kfoE kodira protein koji se bira iz skupine koju čine:
(A) protein koji ima aminokiselinski slijed SEQ ID NO:2;
(B) protein koji ima aminokiselinski slijed SEQ ID NO:2 modificiran delecijom, supstitucijom ili insercijom jedne ili više aminokiselina, te ima aktivnost fruktozil-transferaze; i
(C) protein koji ima aminokiselinski slijed homologan najmanje 50% aminokiselinskom slijedu u SEQ ID NO:2 i ima aktivnost fruktozil-transferaze.
2. Rekombinantni mikroorganizam u skladu s patentnim zahtjevom 1, naznačen time što inaktiviran gen kfoE je a DNA koji se bira iz skupine koju čine:
(a) DNA koja ima nukleotidni slijed SEQ ID NO:1;
(b) DNA koja hibridizira s DNA koja ima nukleotidni slijed komplementaran onom u SEQ ID NO:1 i kodira protein s aktivnošću fruktozil-transferaze; i
(c) DNA koja ima nukleotidni slijed homologan najmanje 50% nukleotidu u SEQ ID NO:1 i kodira protein s aktivnošću fruktozil-transferaze.
3. Rekombinantni mikroorganizam u skladu s bilo kojim od patentnih zahtjeva 1 i 2, naznačen time što je gen kfoE inaktiviran njegovom potpunom ili djelomičnom supstitucijom kasetom za otpornost na kanamicin i njenim naknadni uklanjanjem, što rezultira potpunom ili djelomičnom delecijom gena kfoE.
4. Rekombinantni mikroorganizam u skladu s bilo kojim od patentnih zahtjeva 1 do 3, naznačen time što je izveden iz bakterije koja pripada rodu koji se bira između Escherichia, Haemophilus, Campylobacter, Gloeocapsa i Vibrio.
5. Rekombinantni mikroorganizam u skladu s patentnim zahtjevom 4, naznačen time što je izveden iz bakterije koja pripada vrsti Escherichia coli.
6. Rekombinantni mikroorganizam u skladu s patentnim zahtjevom 5, naznačen time što je izveden iz serotipa vrste Escherichia coli koji pripada skupini 2 antigena K.
7. Rekombinantni mikroorganizam u skladu s patentnim zahtjevom 6, naznačen time što je izveden iz soja Escherichia coli 05:K4:H4U1-41.
8. Rekombinantni mikroorganizam u skladu s bilo kojim od patentnih zahtjeva 1 do 7, naznačen time što je Escherichia coli DSM23578 ili Escherichia coli DSM23644.
9. Rekombinantni mikroorganizam u skladu s patentnim zahtjevom 8, naznačen time što je Escherichia coli DSM23644.
10. Postupak biotehnološke proizvodnje hondroitina, naznačen time što se upotrebljava rekombinantni mikroorganizam odabran između sojeva Escherichia coli DSM23578 i Escherichia coli DSM23644.
11. Postupak u skladu s patentnim zahtjevom 10, naznačen time što je rekombinantni mikroorganizam Escherichia coli DSM23644.
12. Postupak biotehnološke proizvodnje hondroitina, naznačen time što se sastoji u sljedećim koracima:
a. uzgoja u pogodnom mediju rekombinantnog mikroorganizma u skladu s bilo kojim od patentnih zahtjeva 1 do 9;
b. prikupljanja i pročišćavanja hondroitina prisutnog u mikrobiološkoj kulturi.
13. Postupak biotehnološke proizvodnje hondroitina u skladu s patentnim zahtjevom 12, naznačen time što je rekombinantni mikroorganizam Escherichia coli DSM23578 ili Escherichia coli DSM23644.
14. Postupak u skladu s patentnim zahtjevom 13, naznačen time što je rekombinantni mikroorganizam Escherichia coli DSM23644.
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| IT001264A ITMI20101264A1 (it) | 2010-07-09 | 2010-07-09 | Produzione biotecnologica di condroitina |
| IT001300A ITMI20101300A1 (it) | 2010-07-15 | 2010-07-15 | Produzione biotecnologica di condroitina |
| EP11722468.3A EP2591127B1 (en) | 2010-07-09 | 2011-06-01 | Biotechnological production of chondroitin |
| PCT/EP2011/059069 WO2012004063A1 (en) | 2010-07-09 | 2011-06-01 | Biotechnological production of chondroitin |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| HRP20171624T1 true HRP20171624T1 (hr) | 2017-12-01 |
Family
ID=45439053
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| HRP20171624TT HRP20171624T1 (hr) | 2010-07-09 | 2017-10-24 | Biotehnološka proizvodnja hondroitina |
Country Status (25)
| Country | Link |
|---|---|
| US (2) | US8609394B2 (hr) |
| EP (1) | EP2591127B1 (hr) |
| JP (1) | JP6030056B2 (hr) |
| KR (1) | KR101855062B1 (hr) |
| CN (2) | CN103228781B (hr) |
| AU (1) | AU2011275996B2 (hr) |
| BR (1) | BR112013000648A2 (hr) |
| CA (1) | CA2801843C (hr) |
| CY (1) | CY1119469T1 (hr) |
| DK (1) | DK2591127T3 (hr) |
| EA (1) | EA025126B1 (hr) |
| ES (1) | ES2644443T3 (hr) |
| GE (1) | GEP201706720B (hr) |
| HR (1) | HRP20171624T1 (hr) |
| IL (1) | IL223830B (hr) |
| LT (1) | LT2591127T (hr) |
| MX (1) | MX2013000100A (hr) |
| NO (1) | NO2591127T3 (hr) |
| NZ (1) | NZ603990A (hr) |
| PL (1) | PL2591127T3 (hr) |
| PT (1) | PT2591127T (hr) |
| SG (1) | SG186212A1 (hr) |
| SI (1) | SI2591127T1 (hr) |
| WO (1) | WO2012004063A1 (hr) |
| ZA (1) | ZA201209079B (hr) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2542686B1 (en) | 2010-03-01 | 2018-01-03 | Seikagaku Corporation | Compositions and methods for bacterial production of chondroitin |
| US8664196B2 (en) | 2011-05-20 | 2014-03-04 | Gnosis S.P.A. | Shark-like chondroitin sulphate and process for the preparation thereof |
| ITMI20120880A1 (it) | 2012-05-22 | 2013-11-23 | Gnosis Spa | Condroitin 6-solfato biotecnologico a basso peso molecolare dotato di attivita' antiinfiammatoria e antiartritica e suo uso nel trattamento e nella prevenzione dell'osteoartrite |
| JP6478115B2 (ja) | 2013-09-30 | 2019-03-06 | 生化学工業株式会社 | タンパク質の血中滞留性の増強方法 |
| CN106497845B (zh) * | 2016-12-14 | 2019-08-06 | 江南大学 | 一种高产软骨素的重组枯草芽孢杆菌及其应用 |
| US20220333144A1 (en) * | 2019-09-10 | 2022-10-20 | Rensselaer Polytechnic Institute | Recombinant microorganisms for in vivo production of sulfated glycosaminoglycans |
| IT202000004564A1 (it) | 2020-03-04 | 2021-09-04 | Lesaffre & Cie | Processo per la solfatazione diretta di polisaccaridi in solvente ecologicamente accettabile |
| CN112625147A (zh) * | 2020-12-22 | 2021-04-09 | 荣成万盈水产科技有限公司 | 一种利用鱿鱼软骨制备硫酸软骨素的方法 |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7642071B2 (en) | 1999-04-01 | 2010-01-05 | The Board Of Regents Of The University Of Oklahoma | Methods of expressing gram-negative glycosaminoglycan synthase genes in gram-positive hosts |
| AU2003100886B4 (en) | 2003-05-05 | 2004-07-29 | Agnes Chan | A multipurpose garment |
| WO2007069693A1 (ja) | 2005-12-15 | 2007-06-21 | Seikagaku Corporation | 長鎖コンドロイチン糖鎖及びその製造方法並びにコンドロイチン合成の促進方法 |
| US8283145B2 (en) * | 2007-04-24 | 2012-10-09 | Seikagaku Corporation | Chondroitin-producing bacterium and method of producing chondroitin |
| EP2542686B1 (en) | 2010-03-01 | 2018-01-03 | Seikagaku Corporation | Compositions and methods for bacterial production of chondroitin |
-
2011
- 2011-06-01 SI SI201131328T patent/SI2591127T1/sl unknown
- 2011-06-01 PL PL11722468T patent/PL2591127T3/pl unknown
- 2011-06-01 KR KR1020137003182A patent/KR101855062B1/ko active IP Right Grant
- 2011-06-01 EP EP11722468.3A patent/EP2591127B1/en active Active
- 2011-06-01 GE GEAP201112993A patent/GEP201706720B/en unknown
- 2011-06-01 ES ES11722468.3T patent/ES2644443T3/es active Active
- 2011-06-01 CA CA2801843A patent/CA2801843C/en active Active
- 2011-06-01 LT LTEP11722468.3T patent/LT2591127T/lt unknown
- 2011-06-01 NO NO11722468A patent/NO2591127T3/no unknown
- 2011-06-01 BR BR112013000648A patent/BR112013000648A2/pt not_active Application Discontinuation
- 2011-06-01 JP JP2013517135A patent/JP6030056B2/ja active Active
- 2011-06-01 CN CN201180033969.3A patent/CN103228781B/zh active Active
- 2011-06-01 MX MX2013000100A patent/MX2013000100A/es active IP Right Grant
- 2011-06-01 AU AU2011275996A patent/AU2011275996B2/en active Active
- 2011-06-01 SG SG2012089918A patent/SG186212A1/en unknown
- 2011-06-01 EA EA201291455A patent/EA025126B1/ru not_active IP Right Cessation
- 2011-06-01 PT PT117224683T patent/PT2591127T/pt unknown
- 2011-06-01 WO PCT/EP2011/059069 patent/WO2012004063A1/en active Application Filing
- 2011-06-01 DK DK11722468.3T patent/DK2591127T3/da active
- 2011-06-01 NZ NZ603990A patent/NZ603990A/en unknown
- 2011-06-01 CN CN201510177998.7A patent/CN104974972B/zh active Active
- 2011-06-24 US US13/168,289 patent/US8609394B2/en active Active
-
2012
- 2012-11-30 ZA ZA2012/09079A patent/ZA201209079B/en unknown
- 2012-12-24 IL IL223830A patent/IL223830B/en active IP Right Grant
-
2013
- 2013-11-13 US US14/078,809 patent/US8771992B2/en active Active
-
2017
- 2017-10-16 CY CY20171101081T patent/CY1119469T1/el unknown
- 2017-10-24 HR HRP20171624TT patent/HRP20171624T1/hr unknown
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