HRP20160395T1 - Postupci za odvajanje čestica korištenjem aparata s elementom odvajanja koji razlikuje veličine i ima produženi vodeći rub - Google Patents
Postupci za odvajanje čestica korištenjem aparata s elementom odvajanja koji razlikuje veličine i ima produženi vodeći rub Download PDFInfo
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- HRP20160395T1 HRP20160395T1 HRP20160395TT HRP20160395T HRP20160395T1 HR P20160395 T1 HRP20160395 T1 HR P20160395T1 HR P20160395T T HRP20160395T T HR P20160395TT HR P20160395 T HRP20160395 T HR P20160395T HR P20160395 T1 HRP20160395 T1 HR P20160395T1
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- 238000000926 separation method Methods 0.000 title claims 29
- 238000000034 method Methods 0.000 title claims 27
- 239000002245 particle Substances 0.000 title claims 18
- 208000005443 Circulating Neoplastic Cells Diseases 0.000 claims 20
- 206010028980 Neoplasm Diseases 0.000 claims 9
- 210000004369 blood Anatomy 0.000 claims 8
- 239000008280 blood Substances 0.000 claims 8
- 210000002358 circulating endothelial cell Anatomy 0.000 claims 8
- 238000002405 diagnostic procedure Methods 0.000 claims 6
- 238000011144 upstream manufacturing Methods 0.000 claims 6
- 210000000601 blood cell Anatomy 0.000 claims 4
- 239000012530 fluid Substances 0.000 claims 4
- 230000000747 cardiac effect Effects 0.000 claims 3
- 210000004027 cell Anatomy 0.000 claims 3
- 210000003743 erythrocyte Anatomy 0.000 claims 3
- 238000002560 therapeutic procedure Methods 0.000 claims 3
- 206010006187 Breast cancer Diseases 0.000 claims 2
- 208000026310 Breast neoplasm Diseases 0.000 claims 2
- 206010033128 Ovarian cancer Diseases 0.000 claims 2
- 206010061535 Ovarian neoplasm Diseases 0.000 claims 2
- 206010060862 Prostate cancer Diseases 0.000 claims 2
- 208000000236 Prostatic Neoplasms Diseases 0.000 claims 2
- 230000001419 dependent effect Effects 0.000 claims 2
- 230000001605 fetal effect Effects 0.000 claims 2
- 230000002611 ovarian Effects 0.000 claims 2
- 210000002307 prostate Anatomy 0.000 claims 2
- 239000011800 void material Substances 0.000 claims 2
- 241000251539 Vertebrata <Metazoa> Species 0.000 claims 1
- WYTGDNHDOZPMIW-RCBQFDQVSA-N alstonine Natural products C1=CC2=C3C=CC=CC3=NC2=C2N1C[C@H]1[C@H](C)OC=C(C(=O)OC)[C@H]1C2 WYTGDNHDOZPMIW-RCBQFDQVSA-N 0.000 claims 1
- 238000011001 backwashing Methods 0.000 claims 1
- 210000003040 circulating cell Anatomy 0.000 claims 1
- 210000000604 fetal stem cell Anatomy 0.000 claims 1
- 208000014674 injury Diseases 0.000 claims 1
- 208000010125 myocardial infarction Diseases 0.000 claims 1
- 150000007523 nucleic acids Chemical class 0.000 claims 1
- 102000039446 nucleic acids Human genes 0.000 claims 1
- 108020004707 nucleic acids Proteins 0.000 claims 1
- 230000002062 proliferating effect Effects 0.000 claims 1
- 238000011084 recovery Methods 0.000 claims 1
- 239000000725 suspension Substances 0.000 claims 1
- 238000012360 testing method Methods 0.000 claims 1
- 210000001519 tissue Anatomy 0.000 claims 1
- 230000008733 trauma Effects 0.000 claims 1
- 210000004881 tumor cell Anatomy 0.000 claims 1
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502753—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by bulk separation arrangements on lab-on-a-chip devices, e.g. for filtration or centrifugation
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0693—Tumour cells; Cancer cells
- C12N5/0694—Cells of blood, e.g. leukemia cells, myeloma cells
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/24—Methods of sampling, or inoculating or spreading a sample; Methods of physically isolating an intact microorganisms
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/02—Investigating particle size or size distribution
- G01N15/0272—Investigating particle size or size distribution with screening; with classification by filtering
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/483—Physical analysis of biological material
- G01N33/487—Physical analysis of biological material of liquid biological material
- G01N33/49—Blood
- G01N33/491—Blood by separating the blood components
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/06—Fluid handling related problems
- B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
- B01L2200/0652—Sorting or classification of particles or molecules
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/12—Specific details about manufacturing devices
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0809—Geometry, shape and general structure rectangular shaped
- B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
- B01L2300/0864—Configuration of multiple channels and/or chambers in a single devices comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/02—Investigating particle size or size distribution
- G01N2015/0288—Sorting the particles
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- Dispersion Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- General Engineering & Computer Science (AREA)
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- Clinical Laboratory Science (AREA)
- Medicinal Chemistry (AREA)
- Food Science & Technology (AREA)
- Ecology (AREA)
- Hospice & Palliative Care (AREA)
- Fluid Mechanics (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Separation Of Solids By Using Liquids Or Pneumatic Power (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Combined Means For Separation Of Solids (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Claims (27)
1. Uređaj za odvajanje manjih i većih čestica, uređaj obuhvaća
tijelo (2) i
poklopac (4) koji definira prazninu (50) tu između, praznina (50) obuhvaća
element (1) odvajanja koji razdvaja ulazno područje (52) i izlazno područje (58) praznine (50), element odvajanja (1) koji definira, zajedno sa površinom praznine (50), kanal koji fluidno povezuje područje ulaza i područje izlaza putem odvajajućeg dijela, kanal ima
ukupnu širinu na odvajajućem dijelu i
visinu definiranu rastojanjem između elementa (1) odvajanja i površine praznine (50),
najmanje jedno tijelo (2), poklopac (4), i element (1) odvajanja nosi korak odvajanja smješten unutra i ima vodeći rub koja se pruža potpuno preko odvajajućeg dijela kanala, pri čemu se kanal dijeli na uzvodni dio na ulaznoj strani vodećeg ruba i lamelarni nizvodni dio na izlaznoj strani vodećeg ruba,
visina uzvodnog dijela je dovoljna da kroz taj dio olakša prolaz i većim i manjim česticama,
visina nizvodnog dijela je dovoljno velika da olakša prolaz kroz njega manjim česticama i dovoljno mala da većim česticama spriječi prolaz kroz taj donji dio, i
dužina vodećeg ruba je najmanje 20 puta veća od ukupne širine kanala na području odvajanja,
pri čemu gornji dio kanala je lamelaran u području između ulaznog područja (52) i elementa (1) odvajanja , i
pri čemu vodeći rub ima valoviti oblik,
pri čemu čestice mogu biti odvojene propuštanjem kroz kanal, i ponovno dobivanje čestica na osnovu njihove sposobnosti da prelaze stupanj odvajanja.
2. Uređaj prema patentnom zahtjevu 1, pri čemu visina donjeg dijela sprečava prolaz cirkulirajućim stanicama tumora i olakšava prolaz crvenim krvnim zrncima čovjeka.
3. Uređaj prema patentnom zahtjevu 2, pri čemu cirkulirajuće stanice tumora jesu izvedene iz čvrstog tumora kao što je tumor jajnika, prostate ili tumor karcinoma dojke.
4. Uređaj prema jednom od patentnih zahtjeva 1 do 3, pri čemu visina donjeg dijela sprečava prolaz najmanje jedne od: ćelija ljudskog fetusa; ćelija sličnih matičnim ćelijama ljudskog fetusa; cirkulirajućih endotelnih stanica; i uvećanih cirkulirajućih endotelnih stanica kardijalnog porekla, i olakšava prolaz kroz eritrocite čovjeka.
5. Uređaj prema jednom od patentnih zahtjeva 1 do 4, pri čemu najmanje jedno tijelo (2), poklopac (4), i element (1) odvajanja nosi korak fokusiranja smješten unutra i potpuno se pruža preko kanala na ulaznoj strani koraka odvajanja, kanal ima veću visinu od ulazne strane koraka fokusiranja nego na izlaznoj strani; opcionalno, pri čemu se korak fokusiranja proširuje upravno preko kanala.
6. Uređaj prema jednom od patentnih zahtjeva 1 do 5, pri čemu najmanje jedno tijelo (2), poklopac (4), i element (1) odvajanja nosi više koraka odvajanja raspoređenih serijski unutar područja odvajanja, svakog koraka odvajanja:
a) ima vodeći rub koji se pruža preko područja odvajanja i ima dužinu veću od ukupne širine kanala na području odvajanja; i
b) dijeli kanal na uzvodni dio i lamelarni nizvodni dio u odnosu na vodeći rub koraka odvajanja, visina kanala u nizvodnom dijelu neposredno poslije koraka odvajanja je manja od visine kanala u uzvodnom dijelu i neposredno prethodi koraku odvajanja.
7. Uređaj prema jednom od patentnih zahtjeva 1 do 6, pri čemu vodeći rub ima oblik serpentine.
8. Uređaj prema jednom od patentnih zahtjeva 1 do 7, pri čemu stupanj odvajanja ima uzvodnu prednju stranu na svojoj ulaznoj strani koja je uspravna na dio stupnja koji definira nizvodni dio.
9. Uređaj prema jednom od patentnih zahtjeva 1 do 8, pri čemu je element (1) odvajanja integralan sa najmanje jednim od tijela i poklopcem.
10. Uređaj prema jednom od patentnih zahtjeva 1 do 9, da nadalje sadrži nosač za zadržavanje visine kanala, nosač je smješten unutar kanala i pruža se između elementa odvajanja i površine praznine.
11. Uređaj prema jednom od patentnih zahtjeva 1 do 10, pri čemu uređaj ima površinu približno jednaku uobičajenom slajdu mikroskopa.
12. Postupak odvajanja većih i manjih čestica, postupak obuhvaća osiguravanje fluidne suspenzije većih i manjih čestica na ulazu uređaja prema jednom od patentnih zahtjeva 1 do 11, koji potiskuje fluid kroz ovaj kanal i odvajanje čestica na osnovu njihove sposobnosti da prelaze stupanj odvajanja, i opcionalno ponovo dobiju veće čestice na taj način odvojene koje ne mogu proći u izlazno područje (58).
13. Postupak prema patentnom zahtjevu 12, pri čemu postupak još obuhvaća fluid povratnog ispiranja iz izlaznog područja u smjeru ulaza na uređaju da bi se ponovo dobile veće čestice.
14. Postupak prema patentnom zahtjevu 12 ili patentnom zahtjevu 13, pri čemu je fluid uzorak krvi.
15. Postupak prema bilo kom od patentnih zahtjeva 12 do 14, pri čemu veće čestice jesu cirkulirajuće stanice tumora (CTC) a manje čestice su stanice krvi.
16. Postupak prema bilo kom od patentnih zahtjeva 12 do 14, pri čemu veće čestice jesu stanice fetusa a manje čestice jesu stanice krvi.
17. Postupak prema bilo kom od patentnih zahtjeva 12 do 14, pri čemu veće čestice jesu cirkulirajuće endotelne stanice a manje čestice jesu stanice krvi.
18. Postupak prema patentnom zahtjevu 17, pri čemu cirkulirajuće stanice jesu uvećane cirkulirajuće endotelne stanice kardijalnog porijekla.
19. Postupak prema jednom od patentnih zahtjeva 15 do 17, pri čemu stanice krvi jesu crvena krvna zrnca.
20. Postupak procjene efikasnosti terapije tumora za subjekt zahvaćen tumorom, postupak obuhvaća izoliranje CTC iz uzoraka krvi dobivenih od subjekta prije i posle terapije korištenjem postupka prema patentnom zahtjevu 15 ili patentom zahtjevu 19 kada zavisi od patentnog zahtjeva 15 i uspoređivanje najmanje jedne karakteristike cirkulirajuće stanice tumora izolirane iz uzoraka, pri čemu razlika u karakteristikama cirkulirajućih stanica tumora izoliranih iz uzoraka krvi je indikacija efikasnosti terapije; opcionalno,
pri čemu ova karakteristika je koncentracija cirkulirajućih stanica tumora u uzorku.
21. Postupak dijagnosticiranja pojave tumora kod kičmenjaka, postupak sadrži odvajanje cirkulirajućih stanica tumora (CTC) iz uzorka krvi dobivene od subjekta korištenjem postupka prema patentnom zahtjevu 15 ili patentnom zahtjevu 19 kada je zavisan od patentnog zahtjeva 15,
pri čemu visina nizvodnog dijela sprečava prolaz za cirkulirajuće stanice tumora (CTC) kroz taj dio,
pri čemu prisustvo najmanje jedne odvojene cirkulirajuće stanice tumora je indikacija da tumor postoji kod subjekta.
22. Postupak prema patentnom zahtjevu 21, još obuhvaća ispitivanje dijela aparata u uzvodnom dijelu aparata vodeće ivice stupanja odvajanja za prisustvo najmanje jedne odvojene cirkulirajuće stanice tumora (CTC).
23. Postupak prema patentnom zahtjevu 21 ili patentnom zahtjevu 22, još obuhvaća naknadno izvođenje testa dijagnostike koji procjenjuje karakteristiku stanice tumora na barem jednoj odvojenoj cirkulirajućoj stanici tumora koja je bila prisutna.
24. Postupak za koji se zaštita patentom traži u patentnom zahtjevu 23, pri čemu se dijagnostičko ispitivanje vezuje sa antitijelom specifičnim za tkivo; I/ILI, pri čemu se dijagnostičko ispitivanje vezuje sa antitijelom specifičnim za tumor; I/ILI,
pri čemu dijagnostičko ispitivanje obuhvaća analiziranje nukleinskih kiselina dobivenih iz najmanje jedne odvojene cirkulirajuće stanice tumora koja je bila prisutna; I/ILI,
pri čemu dijagnostičko ispitivanje je procjena proliferativnog kapaciteta odvojene cirkulirajuće stanice tumora; I/ILI,
pri čemu dijagnostičko ispitivanje je mikroskopsko promatranje morfologije odvojene cirkulirajuće stanice tumora.
25. Postupak za koji se traži zaštita u jednom od patentnih zahtjeva 21 do 24, pri čemu je najmanje jedna cirkulirajuća stanica tumora izvedena iz čvrstog tumora kao što je tumor jajnika, prostate, ili tumor karcinoma dojke.
26. Postupak određivanja indikacije prisustva ili pojave traume kod pojedinca na osnovu prisustva cirkulirajućih endotelnih stanica u uzorku krvi, postupak obuhvaća odvajanje cirkulirajućih endotelnih stanica iz uzorka krvi dobivene od pojedinca korištenjem postupka prema patentnom zahtjevu 17 ili patentnom zahtjevu 19 kada je zavisan od patentnog zahtjeva 17.
27. Postupak određivanja indikacije da je pacijent nedavno bio izložen, ili je trenutno izložen, ili je u neposrednoj opasnosti za pojavu miokardijalnog infarkta na osnovu prisustva uvećanih cirkulirajućih endotelnih stanica kardijalnog porijekla u uzorku krvi, postupak obuhvaća odvajanje uvećanih cirkulirajućih endotelnih stanica iz uzorka krvi dobivene od pacijenta korištenjem postupka prema patentnom zahtjevu 18.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201361794468P | 2013-03-15 | 2013-03-15 | |
| US14/077,811 US9631179B2 (en) | 2013-03-15 | 2013-11-12 | Methods for segregating particles using an apparatus with a size-discriminating separation element having an elongate leading edge |
| EP14159725.2A EP2790020B9 (en) | 2013-03-15 | 2014-03-14 | Methods for segregating particles using an apparatus with a size-discriminating separation element having an elongate leading edge |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| HRP20160395T1 true HRP20160395T1 (hr) | 2016-07-01 |
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Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| HRP20160395TT HRP20160395T1 (hr) | 2013-03-15 | 2016-04-18 | Postupci za odvajanje čestica korištenjem aparata s elementom odvajanja koji razlikuje veličine i ima produženi vodeći rub |
| HRP20170360TT HRP20170360T1 (hr) | 2013-03-15 | 2017-03-06 | Postupci za odvajanje čestica korištenjem aparata s elementom odvajanja koji razlikuje veličine i ima produženi vodeći rub |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| HRP20170360TT HRP20170360T1 (hr) | 2013-03-15 | 2017-03-06 | Postupci za odvajanje čestica korištenjem aparata s elementom odvajanja koji razlikuje veličine i ima produženi vodeći rub |
Country Status (20)
| Country | Link |
|---|---|
| US (6) | US9631179B2 (hr) |
| EP (2) | EP3029465B1 (hr) |
| JP (2) | JP6066950B2 (hr) |
| CN (3) | CN107991476B (hr) |
| AU (2) | AU2014201576B2 (hr) |
| BR (1) | BR102014006329A2 (hr) |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CA2796237C (en) | 2010-04-27 | 2016-11-22 | Halliburton Energy Services, Inc. | Fracture analysis |
| US9631179B2 (en) * | 2013-03-15 | 2017-04-25 | Angle North America, Inc. | Methods for segregating particles using an apparatus with a size-discriminating separation element having an elongate leading edge |
| JP6726680B2 (ja) * | 2015-03-16 | 2020-07-22 | ルミネックス コーポレーション | 多段チャネル乳化のための装置及び方法 |
| GB201617723D0 (en) | 2016-10-19 | 2016-11-30 | Univ London Queen Mary | Method for predicting prostate cancer metastasis |
| GB201617722D0 (en) | 2016-10-19 | 2016-11-30 | Univ London Queen Mary | Method for determining prognosis of cancer |
| CN107854936B (zh) * | 2017-12-02 | 2023-06-20 | 西部新锆核材料科技有限公司 | 一种生物雾霾防治工作站 |
| WO2019131173A1 (ja) * | 2017-12-28 | 2019-07-04 | 株式会社村田製作所 | 分離回収システム及び分離回収方法 |
| FR3087792B1 (fr) * | 2018-10-29 | 2023-09-29 | Inovotion | Modele animal pour amplifier des cellules tumorales circulantes humaines ou animales |
| EP3897657A2 (en) | 2018-12-20 | 2021-10-27 | Universität Basel | <sup2/>? <sub2/>?+? ?na/k <ns1:sup>+</ns1:sup>?atpase inhibitors for use in the prevention or treatment of metastasis |
| CN109557304A (zh) * | 2019-01-11 | 2019-04-02 | 广州万孚生物技术股份有限公司 | 免疫层析检测装置 |
| WO2020178951A1 (en) * | 2019-03-04 | 2020-09-10 | Enplas Corporation | Fluid handling device |
| KR102569747B1 (ko) * | 2020-04-27 | 2023-08-25 | 고려대학교 산학협력단 | 유동채널 및 체액 내 입자 분리 장치 |
| CN114397141B (zh) * | 2021-12-10 | 2022-11-29 | 连云港市东方医院 | 一种神经内科临床用取液装置 |
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| US5744366A (en) | 1992-05-01 | 1998-04-28 | Trustees Of The University Of Pennsylvania | Mesoscale devices and methods for analysis of motile cells |
| US5486335A (en) | 1992-05-01 | 1996-01-23 | Trustees Of The University Of Pennsylvania | Analysis based on flow restriction |
| US5637469A (en) | 1992-05-01 | 1997-06-10 | Trustees Of The University Of Pennsylvania | Methods and apparatus for the detection of an analyte utilizing mesoscale flow systems |
| US5726026A (en) | 1992-05-01 | 1998-03-10 | Trustees Of The University Of Pennsylvania | Mesoscale sample preparation device and systems for determination and processing of analytes |
| US5296375A (en) | 1992-05-01 | 1994-03-22 | Trustees Of The University Of Pennsylvania | Mesoscale sperm handling devices |
| FR2824144B1 (fr) * | 2001-04-30 | 2004-09-17 | Metagenex S A R L | Methode de diagnostic prenatal sur cellule foetale isolee du sang maternel |
| US7993908B2 (en) * | 2001-07-17 | 2011-08-09 | Parsortix, Inc. | Microstructure for particle and cell separation, identification, sorting, and manipulation |
| EP1671101A1 (en) * | 2003-10-03 | 2006-06-21 | Vrije Universiteit Brussel | Method and device for size-separating particles present in a fluid |
| US7846743B2 (en) * | 2005-04-21 | 2010-12-07 | California Institute Of Technology | Uses of parylene membrane filters |
| GB0508983D0 (en) * | 2005-05-03 | 2005-06-08 | Oxford Gene Tech Ip Ltd | Cell analyser |
| EP1795894A1 (en) * | 2005-12-06 | 2007-06-13 | Roche Diagnostics GmbH | Plasma separation on a disk like device |
| US8036773B2 (en) * | 2006-05-10 | 2011-10-11 | Mckesson Automation Inc. | System, method and corresponding apparatus for storing, retrieving and delivering unit dose blisters |
| WO2008130032A1 (ja) * | 2007-04-19 | 2008-10-30 | The Ritsumeikan Trust | 液中粒子分級装置及び液中粒子分級方法、粒径測定装置及び粒径測定方法 |
| WO2011028483A2 (en) | 2009-08-24 | 2011-03-10 | Parsortix, Inc. | Methods and apparatus for segregation of particles, including additional sources of sample collection for separation of nucleated fetal cells |
| AU2010246381B2 (en) * | 2008-04-23 | 2013-08-15 | Angle North America, Inc. | Methods and apparatus for segregation of particles |
| CN102083997B (zh) * | 2008-04-23 | 2016-05-25 | 角度北美公司 | 用于分离颗粒的方法和设备 |
| EP2304414B1 (en) | 2008-07-24 | 2017-03-15 | The Trustees of Princeton University | Bump array device having asymmetric gaps for segregation of particles |
| WO2010129441A2 (en) | 2009-05-04 | 2010-11-11 | Gpb Scientific, Llc | Method for separating stem cells from their more differentiated progeny using microfluidic devices |
| NL1038359C2 (en) * | 2010-03-31 | 2012-06-27 | Aquamarijn Res B V | Device and method for separation of circulating tumor cells. |
| CA2833026A1 (en) | 2011-04-15 | 2012-10-18 | The University Of British Columbia | Method and apparatus for separation of particles |
| US9631179B2 (en) | 2013-03-15 | 2017-04-25 | Angle North America, Inc. | Methods for segregating particles using an apparatus with a size-discriminating separation element having an elongate leading edge |
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