HRP20151244T1 - Postupak za pripremu modificiranih elektroda, elektrode pripremljene tim postupkom i enzimski biosenzori koji sadrže te elektrode - Google Patents
Postupak za pripremu modificiranih elektroda, elektrode pripremljene tim postupkom i enzimski biosenzori koji sadrže te elektrode Download PDFInfo
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- HRP20151244T1 HRP20151244T1 HRP20151244TT HRP20151244T HRP20151244T1 HR P20151244 T1 HRP20151244 T1 HR P20151244T1 HR P20151244T T HRP20151244T T HR P20151244TT HR P20151244 T HRP20151244 T HR P20151244T HR P20151244 T1 HRP20151244 T1 HR P20151244T1
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- 238000000034 method Methods 0.000 title claims 21
- 238000002360 preparation method Methods 0.000 title claims 2
- 230000002255 enzymatic effect Effects 0.000 title 1
- 239000000243 solution Substances 0.000 claims 18
- 108090000790 Enzymes Proteins 0.000 claims 8
- 102000004190 Enzymes Human genes 0.000 claims 8
- 229940088598 enzyme Drugs 0.000 claims 8
- 239000004094 surface-active agent Substances 0.000 claims 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims 5
- CWYNVVGOOAEACU-UHFFFAOYSA-N iron (II) ion Substances [Fe+2] CWYNVVGOOAEACU-UHFFFAOYSA-N 0.000 claims 4
- DCYOBGZUOMKFPA-UHFFFAOYSA-N iron(2+);iron(3+);octadecacyanide Chemical group [Fe+2].[Fe+2].[Fe+2].[Fe+3].[Fe+3].[Fe+3].[Fe+3].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-] DCYOBGZUOMKFPA-UHFFFAOYSA-N 0.000 claims 4
- -1 polyoxyethylene Polymers 0.000 claims 4
- 229960003351 prussian blue Drugs 0.000 claims 4
- 239000013225 prussian blue Substances 0.000 claims 4
- 150000003839 salts Chemical class 0.000 claims 4
- VTLYFUHAOXGGBS-UHFFFAOYSA-N Fe3+ Chemical compound [Fe+3] VTLYFUHAOXGGBS-UHFFFAOYSA-N 0.000 claims 3
- 239000003929 acidic solution Substances 0.000 claims 3
- 238000001035 drying Methods 0.000 claims 3
- 238000005406 washing Methods 0.000 claims 3
- 229910021578 Iron(III) chloride Inorganic materials 0.000 claims 2
- 239000012491 analyte Substances 0.000 claims 2
- 239000013060 biological fluid Substances 0.000 claims 2
- YAGKRVSRTSUGEY-UHFFFAOYSA-N ferricyanide Chemical compound [Fe+3].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-] YAGKRVSRTSUGEY-UHFFFAOYSA-N 0.000 claims 2
- 150000002500 ions Chemical class 0.000 claims 2
- RBTARNINKXHZNM-UHFFFAOYSA-K iron trichloride Chemical compound Cl[Fe](Cl)Cl RBTARNINKXHZNM-UHFFFAOYSA-K 0.000 claims 2
- VCJMYUPGQJHHFU-UHFFFAOYSA-N iron(3+);trinitrate Chemical compound [Fe+3].[O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O VCJMYUPGQJHHFU-UHFFFAOYSA-N 0.000 claims 2
- 150000007522 mineralic acids Chemical class 0.000 claims 2
- 239000000203 mixture Substances 0.000 claims 2
- 239000002904 solvent Substances 0.000 claims 2
- QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 claims 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims 1
- FBPFZTCFMRRESA-FSIIMWSLSA-N D-Glucitol Natural products OC[C@H](O)[C@H](O)[C@@H](O)[C@H](O)CO FBPFZTCFMRRESA-FSIIMWSLSA-N 0.000 claims 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 claims 1
- 108010093096 Immobilized Enzymes Proteins 0.000 claims 1
- 229920003171 Poly (ethylene oxide) Polymers 0.000 claims 1
- 229920001213 Polysorbate 20 Polymers 0.000 claims 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims 1
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 claims 1
- 125000000129 anionic group Chemical group 0.000 claims 1
- 229960000686 benzalkonium chloride Drugs 0.000 claims 1
- CADWTSSKOVRVJC-UHFFFAOYSA-N benzyl(dimethyl)azanium;chloride Chemical compound [Cl-].C[NH+](C)CC1=CC=CC=C1 CADWTSSKOVRVJC-UHFFFAOYSA-N 0.000 claims 1
- 230000015572 biosynthetic process Effects 0.000 claims 1
- 125000002091 cationic group Chemical group 0.000 claims 1
- 239000010941 cobalt Substances 0.000 claims 1
- 229910017052 cobalt Inorganic materials 0.000 claims 1
- GUTLYIVDDKVIGB-UHFFFAOYSA-N cobalt atom Chemical compound [Co] GUTLYIVDDKVIGB-UHFFFAOYSA-N 0.000 claims 1
- 150000001875 compounds Chemical class 0.000 claims 1
- 235000014113 dietary fatty acids Nutrition 0.000 claims 1
- 239000012153 distilled water Substances 0.000 claims 1
- ICHUMRJJFKQBGK-UHFFFAOYSA-N dodecyl ethoxy sulfate Chemical compound CCCCCCCCCCCCOS(=O)(=O)OOCC ICHUMRJJFKQBGK-UHFFFAOYSA-N 0.000 claims 1
- 229930195729 fatty acid Natural products 0.000 claims 1
- 239000000194 fatty acid Substances 0.000 claims 1
- 239000008103 glucose Substances 0.000 claims 1
- 235000019420 glucose oxidase Nutrition 0.000 claims 1
- 125000002791 glucosyl group Chemical group C1([C@H](O)[C@@H](O)[C@H](O)[C@H](O1)CO)* 0.000 claims 1
- 229910002804 graphite Inorganic materials 0.000 claims 1
- 239000010439 graphite Substances 0.000 claims 1
- 159000000014 iron salts Chemical class 0.000 claims 1
- UETZVSHORCDDTH-UHFFFAOYSA-N iron(2+);hexacyanide Chemical compound [Fe+2].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-] UETZVSHORCDDTH-UHFFFAOYSA-N 0.000 claims 1
- RUTXIHLAWFEWGM-UHFFFAOYSA-H iron(3+) sulfate Chemical compound [Fe+3].[Fe+3].[O-]S([O-])(=O)=O.[O-]S([O-])(=O)=O.[O-]S([O-])(=O)=O RUTXIHLAWFEWGM-UHFFFAOYSA-H 0.000 claims 1
- 229910000360 iron(III) sulfate Inorganic materials 0.000 claims 1
- 239000000463 material Substances 0.000 claims 1
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 claims 1
- 229910052700 potassium Inorganic materials 0.000 claims 1
- 239000011591 potassium Substances 0.000 claims 1
- 238000002203 pretreatment Methods 0.000 claims 1
- 239000012487 rinsing solution Substances 0.000 claims 1
- 229910052708 sodium Inorganic materials 0.000 claims 1
- 239000011734 sodium Substances 0.000 claims 1
- 235000019333 sodium laurylsulphate Nutrition 0.000 claims 1
- 239000000600 sorbitol Substances 0.000 claims 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/001—Enzyme electrodes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/001—Enzyme electrodes
- C12Q1/002—Electrode membranes
- C12Q1/003—Functionalisation
-
- C—CHEMISTRY; METALLURGY
- C23—COATING METALLIC MATERIAL; COATING MATERIAL WITH METALLIC MATERIAL; CHEMICAL SURFACE TREATMENT; DIFFUSION TREATMENT OF METALLIC MATERIAL; COATING BY VACUUM EVAPORATION, BY SPUTTERING, BY ION IMPLANTATION OR BY CHEMICAL VAPOUR DEPOSITION, IN GENERAL; INHIBITING CORROSION OF METALLIC MATERIAL OR INCRUSTATION IN GENERAL
- C23C—COATING METALLIC MATERIAL; COATING MATERIAL WITH METALLIC MATERIAL; SURFACE TREATMENT OF METALLIC MATERIAL BY DIFFUSION INTO THE SURFACE, BY CHEMICAL CONVERSION OR SUBSTITUTION; COATING BY VACUUM EVAPORATION, BY SPUTTERING, BY ION IMPLANTATION OR BY CHEMICAL VAPOUR DEPOSITION, IN GENERAL
- C23C18/00—Chemical coating by decomposition of either liquid compounds or solutions of the coating forming compounds, without leaving reaction products of surface material in the coating; Contact plating
- C23C18/02—Chemical coating by decomposition of either liquid compounds or solutions of the coating forming compounds, without leaving reaction products of surface material in the coating; Contact plating by thermal decomposition
- C23C18/12—Chemical coating by decomposition of either liquid compounds or solutions of the coating forming compounds, without leaving reaction products of surface material in the coating; Contact plating by thermal decomposition characterised by the deposition of inorganic material other than metallic material
- C23C18/1204—Chemical coating by decomposition of either liquid compounds or solutions of the coating forming compounds, without leaving reaction products of surface material in the coating; Contact plating by thermal decomposition characterised by the deposition of inorganic material other than metallic material inorganic material, e.g. non-oxide and non-metallic such as sulfides, nitrides based compounds
-
- C—CHEMISTRY; METALLURGY
- C23—COATING METALLIC MATERIAL; COATING MATERIAL WITH METALLIC MATERIAL; CHEMICAL SURFACE TREATMENT; DIFFUSION TREATMENT OF METALLIC MATERIAL; COATING BY VACUUM EVAPORATION, BY SPUTTERING, BY ION IMPLANTATION OR BY CHEMICAL VAPOUR DEPOSITION, IN GENERAL; INHIBITING CORROSION OF METALLIC MATERIAL OR INCRUSTATION IN GENERAL
- C23C—COATING METALLIC MATERIAL; COATING MATERIAL WITH METALLIC MATERIAL; SURFACE TREATMENT OF METALLIC MATERIAL BY DIFFUSION INTO THE SURFACE, BY CHEMICAL CONVERSION OR SUBSTITUTION; COATING BY VACUUM EVAPORATION, BY SPUTTERING, BY ION IMPLANTATION OR BY CHEMICAL VAPOUR DEPOSITION, IN GENERAL
- C23C18/00—Chemical coating by decomposition of either liquid compounds or solutions of the coating forming compounds, without leaving reaction products of surface material in the coating; Contact plating
- C23C18/16—Chemical coating by decomposition of either liquid compounds or solutions of the coating forming compounds, without leaving reaction products of surface material in the coating; Contact plating by reduction or substitution, e.g. electroless plating
- C23C18/1601—Process or apparatus
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54366—Apparatus specially adapted for solid-phase testing
- G01N33/54373—Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
- G01N33/5438—Electrodes
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
- Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
- Y10T436/143333—Saccharide [e.g., DNA, etc.]
- Y10T436/144444—Glucose
Claims (23)
1. Postupak za pripremu tiskane mrežaste elektrode modificirane sa Prusko plavim, naznačen time, što se sastoji od uzastopnog nanošenja po površini spomenute tiskane mrežaste elektrode otopine koju čine ioni željeza(III) ili željeza(II) i najmanje jedan površinski aktivni agens, u odgovarajućem otapalu i otopini koju čine ioni ferocijanida(II) ili fericijanida(III) i najmanje jedan površinski aktivni agens, u odgovarajućem otapalu, a spomenute otopine imaju takve koncentracije da dolazi do stvaranja Prusko plavog direktno na površini elektrode; a pri tome se ove otopine nanose u jednakom volumenu i jednakoj koncentraciji; zatim se ovako modificirana elektroda ostavi u sušionici da se suši na temperaturi između 50°C i 200°C, tokom razdoblja između 10 min i 3 h; a spomenuti površinski aktivni agens se bira iz grupe koju čine natrij-lauril sulfat i lauril etoksi sulfat, benzalkonijev klorid, derivati polioksietilena i estera masnih kiselina sa sorbitolom, i njihove smjese.
2. Postupak prema Zahtjevu 1, naznačen time, što se spomenuta tiskana mrežasta elektroda ne podvrgava pred-tretmanu, prije spomenutog uzastopnog nanošenja otopine.
3. Postupak prema Zahtjevu 1, naznačen time, što je spomenuta otopina, koja sadrži ione željeza(III) ili željeza(II), u kiseloj otopini, sa pH između 0.5 i 6.0, a spoj se bira iz grupe koju čine soli željeza(III) ili željeza(II) sa neorganskim kiselinama.
4. Postupak prema Zahtjevu 3, naznačen time, što je spomenuta sol željeza(III) ili željeza(II) sol neorganskih kiselina, koja se bira između feri-klorida, feri-sulfata i feri-nitrata.
5. Postupak prema Zahtjevu 3, naznačen time, što spomenuta otopina predstavlja feri-klorid u 0,01 M HCI u vodi, koji ima pH 2.0.
6. Postupak prema Zahtjevu 1, naznačen time, što se spomenuta otopina, koja se sastoji od iona heksacijanoferata(III), predstavlja kiselu otopinu sa pH između pH 0.5 i 6.0, koji sadrži sol iona ferocijanida(II) ili fericijanida(III).
7. Postupak prema Zahtjevu 6, naznačen time, što se spomenuta sol bira između natrij-, kalij-, amonij- i kobalt-heksacijanoferata.
8. Postupak prema Zahtjevu 6, naznačen time, što spomenuta otopina predstavlja otopinu kalij-fericijanida, K3Fe(CN)6, u 0,01 M otopinu HCl u vodi, koja ima pH 2.0.
9. Postupak prema Zahtjevu 1, naznačen time, što spomenute otopine imaju jednaku koncentraciju, između 20 mM i 2 mM, a nanose se u jednakim volumenima, između 100 nL i 4 mL, po elektrodnoj površini koja je između 0.314 mm2 i 3.14 cm2, poželjno 3.14 mm2.
10. Postupak prema Zahtjevu 1, naznačen time, što je spomenuta tiskana mrežasta elektroda napravljena od inertnog materijala, čija je mrežica tiskana grafitnom tintom.
11. Postupak prema Zahtjevu 1, koji se sastoji još od koraka ostavljanja spomenutih nanesenih otopina da se odmaraju 10 min, ispiranja površine radne elektrode otopinom za ispiranje, koja se sastoji od neke kisele otopine u vodi, sa pH između 0.5 i 6.0, po potrebi ispiranja destiliranom vodom i ostavljanja u sušionici da se suši na temperaturi između 50°C i 200°C, tokom vremena između 10 min i 3 h.
12. Postupak prema Zahtjevu 11, naznačen time, što spomenuta otopina za ispiranje predstavlja 0,01 M otopinu HCl u vodi, koja ima pH 2.0.
13. Postupak prema Zahtjevu 12, naznačen time, što se elektroda ostavlja u sušionici da se suši na temperaturi 100°C, tokom vremena od 1 h 30 min.
14. Postupak prema Zahtjevu 1, naznačen time, što se spomenuti površinski aktivni agens bira između kationskih, anionskih, amfoternih površinski aktivnih agenasa i njihovih smjesa, a dodaje se svakoj otopini u sadržaju između 0.001 i 10 mas%, računano na ukupni volumen otopine.
15. Postupak prema Zahtjevu 1, naznačen time, što spomenuti površinski aktivni agens predstavlja polioksietilen (20) sorbitan monolaurat, u sadržaju od 0.05 mas%, računano na ukupni volumen otopine.
16. Tiskana mrežasta elektroda, modificirana Prusko plavim, pripremljena postupkom koji je određen u Zahtjevima 1-15, naznačena time, što količina Prusko plavog iznosi 100 nmol/cm2.
17. Enzimska elektroda, koja se sastoji od tiskane mrežaste elektrode, određene u Zahtjevu 16, i pogodnog enzima imobiliziranog na spomenutoj elektrodi, a bira se tako da se njen proizvod može elektrokemijski oksidirati ili reducirati na spomenutoj modificiranoj elektrodi.
18. Enzimska elektroda prema Zahtjevu 17, naznačena time, što spomenuti enzim predstavlja enzim oksidaze glukoze.
19. Enzimski biosenzor, koji se sastoji od enzimske elektrode određene Zahtjevima 17-18 kao radne elektrode, neke referentne elektrode, ukoliko je potrebno i neke kontra-elektrode i neke stanice, za prihvaćanje biološkog fluida koji treba analizirati, tako da ovaj posljednji dolazi u kontakt sa enzimom, imobiliziranim na spomenutoj enzimskoj elektrodi.
20. Biosenzor prema Zahtjevu 19, koji se sastoji od spomenute tiskane mrežaste elektrode i spomenute referentne elektrode, kao jedinih elektroda.
21. Postupak za određivanje količine nekog analita u nekom biološkom fluidu, koji se sastoji od zadavanja odgovarajuće vrijednosti potencijala između enzimske elektrode, određene Zahtjevima 17-18 i neke referentne elektrode, obuhvaćene u biosenzoru, koji je određen Zahtjevima 19-20, i očitavanja generiranog strujnog signala.
22. Postupak prema Zahtjevu 21, naznačen time, što spomenuti analit predstavlja glukoza, a spomenuti zadani potencijal je između -250 i +200 mV.
23. Postupak prema Zahtjevu 22, naznačen time, što je spomenuti zadani potencijal jednak -50 mV.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| IT000322A ITFI20060322A1 (it) | 2006-12-13 | 2006-12-13 | Processo per la preparazione di elettrodi modificati, elettrodi preparati con tale processo, e biosensori enzimatici che li comprendono. |
| EP07122884.5A EP1935331B1 (en) | 2006-12-13 | 2007-12-11 | Process for the preparation of modified electrodes, electrodes prepared with said process, and enzymatic biosensors comprising said electrodes |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| HRP20151244T1 true HRP20151244T1 (hr) | 2016-01-29 |
Family
ID=39272068
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| HRP20151244TT HRP20151244T1 (hr) | 2006-12-13 | 2015-11-18 | Postupak za pripremu modificiranih elektroda, elektrode pripremljene tim postupkom i enzimski biosenzori koji sadrže te elektrode |
Country Status (14)
| Country | Link |
|---|---|
| US (2) | US8309362B2 (hr) |
| EP (1) | EP1935331B1 (hr) |
| CY (1) | CY1117023T1 (hr) |
| DK (1) | DK1935331T3 (hr) |
| ES (1) | ES2555313T3 (hr) |
| HR (1) | HRP20151244T1 (hr) |
| HU (1) | HUE026692T2 (hr) |
| IT (1) | ITFI20060322A1 (hr) |
| ME (1) | ME02268B (hr) |
| PL (1) | PL1935331T3 (hr) |
| PT (1) | PT1935331E (hr) |
| RS (1) | RS54399B1 (hr) |
| RU (1) | RU2442130C2 (hr) |
| SI (1) | SI1935331T1 (hr) |
Families Citing this family (27)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7905134B2 (en) * | 2002-08-06 | 2011-03-15 | The Regents Of The University Of California | Biomarker normalization |
| US7810380B2 (en) | 2003-03-25 | 2010-10-12 | Tearlab Research, Inc. | Systems and methods for collecting tear film and measuring tear film osmolarity |
| GB0813200D0 (en) * | 2008-07-18 | 2008-08-27 | Univ Leeds | Hexacyanoferrate modified electrode |
| CN101825603A (zh) * | 2010-04-17 | 2010-09-08 | 上海交通大学 | 用于检测触酶阳性菌的电流型酶电极及其制备方法 |
| US9097661B2 (en) * | 2011-01-27 | 2015-08-04 | Caterpillar, Inc. | Electrochemical sulfur sensor and the method of making the same |
| RU2580288C2 (ru) * | 2013-04-29 | 2016-04-10 | Общество С Ограниченной Ответственностью "Русенс" | Способ изготовления микробиосенсора для определения глюкозы или лактата |
| CN104198554B (zh) * | 2014-09-03 | 2016-06-15 | 海南大学 | 一种工作电极及其制备方法、生物传感器 |
| US11536707B2 (en) | 2014-09-23 | 2022-12-27 | Tearlab Research, Inc. | Systems and methods for integration of microfluidic tear collection and lateral flow analysis of analytes of interest |
| KR101633057B1 (ko) | 2015-04-22 | 2016-06-23 | 재단법인 실감교류인체감응솔루션연구단 | 헤드 마운트 디스플레이 시스템을 위한 얼굴 모션 캡쳐 방법 |
| WO2017210465A1 (en) * | 2016-06-01 | 2017-12-07 | Autotelic Llc | Prussian blue screen-printed electrode for determining cation concentration in physiological samples |
| CN109312384B (zh) | 2016-06-15 | 2022-12-30 | 伊士曼化工公司 | 物理气相沉积的生物传感器组件 |
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| WO2018052711A1 (en) | 2016-09-16 | 2018-03-22 | Eastman Chemical Company | Biosensor electrodes prepared by physical vapor deposition |
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| RU2696499C1 (ru) * | 2018-02-08 | 2019-08-02 | Федеральное государственное бюджетное образовательное учреждение высшего образования "Московский государственный университет имени М.В. Ломоносова" (МГУ) | Биосенсор для одновременного определения глюкозы и лактата в крови |
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| US11536685B2 (en) | 2020-02-06 | 2022-12-27 | Trustees Of Boston University | High throughput assay for identifying microbial redox enzymes |
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| CN115072740B (zh) * | 2022-06-23 | 2023-10-10 | 齐齐哈尔大学 | 一种核壳结构普鲁士蓝纳米球的制备方法 |
| CN115266865B (zh) * | 2022-07-29 | 2024-04-16 | 北京大学 | 一种提高电化学传感器稳定性的方法 |
| CN115494130A (zh) * | 2022-09-19 | 2022-12-20 | 北京大学 | 一种牙刷电化学传感器构建方法 |
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| JPS5858288A (ja) | 1981-10-02 | 1983-04-06 | Seiko Instr & Electronics Ltd | ヘキサシアノ鉄酸鉄の合成法 |
| US4652530A (en) * | 1985-07-15 | 1987-03-24 | Rohm And Haas Company | Monitoring method for isothiazolones in aqueous systems |
| EP0231476A1 (en) * | 1985-12-23 | 1987-08-12 | Siddiqi, Iqbal W., Dr. | Selectively ion-permeable electrodes for analyzing selected ions in aqueous solution |
| EP1390733B1 (en) * | 2001-05-30 | 2010-05-19 | i-SENS, INC. | Biosensor |
| KR100475634B1 (ko) * | 2001-12-24 | 2005-03-15 | 주식회사 아이센스 | 일정 소량의 시료를 빠르게 도입할 수 있는 시료도입부를구비한 바이오 센서 |
| US20060205090A1 (en) * | 2005-03-14 | 2006-09-14 | Newton Michael W | Water-soluble conjugates for electrochemical detection |
| US20100000882A1 (en) * | 2006-10-04 | 2010-01-07 | Arizona Board Of Regents For And On Behalf Of Arizona State University | Detection of Explosive Materials |
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2006
- 2006-12-13 IT IT000322A patent/ITFI20060322A1/it unknown
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- 2007-12-11 HU HUE07122884A patent/HUE026692T2/en unknown
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| US20130001102A1 (en) | 2013-01-03 |
| RS54399B1 (en) | 2016-04-28 |
| ME02268B (me) | 2016-04-28 |
| PT1935331E (pt) | 2016-01-11 |
| US8309362B2 (en) | 2012-11-13 |
| EP1935331B1 (en) | 2015-09-09 |
| SI1935331T1 (sl) | 2016-04-29 |
| EP1935331A3 (en) | 2014-01-01 |
| CY1117023T1 (el) | 2017-04-05 |
| ES2555313T3 (es) | 2015-12-30 |
| PL1935331T3 (pl) | 2016-06-30 |
| US20080160625A1 (en) | 2008-07-03 |
| RU2007146458A (ru) | 2009-06-20 |
| ITFI20060322A1 (it) | 2008-06-14 |
| EP1935331A2 (en) | 2008-06-25 |
| DK1935331T3 (en) | 2015-12-14 |
| HUE026692T2 (en) | 2016-07-28 |
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