HK1220733A1 - 2’-终止子有关的焦磷酸解作用激活的聚合 - Google Patents

2’-终止子有关的焦磷酸解作用激活的聚合 Download PDF

Info

Publication number
HK1220733A1
HK1220733A1 HK16108797.0A HK16108797A HK1220733A1 HK 1220733 A1 HK1220733 A1 HK 1220733A1 HK 16108797 A HK16108797 A HK 16108797A HK 1220733 A1 HK1220733 A1 HK 1220733A1
Authority
HK
Hong Kong
Prior art keywords
polymerase
nucleic acid
nucleotide
oligonucleotide
primer
Prior art date
Application number
HK16108797.0A
Other languages
English (en)
Chinese (zh)
Inventor
Harrow Gelfand David
A. Bauer Keith
P. Gupta Amar
Bodepudi Veeraiah
Niemiec John
Original Assignee
F. Hoffmann-La Roche Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by F. Hoffmann-La Roche Ag filed Critical F. Hoffmann-La Roche Ag
Publication of HK1220733A1 publication Critical patent/HK1220733A1/zh

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Pathology (AREA)
  • Hospice & Palliative Care (AREA)
  • Oncology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Polymers With Sulfur, Phosphorus Or Metals In The Main Chain (AREA)
HK16108797.0A 2006-10-18 2016-07-22 2’-终止子有关的焦磷酸解作用激活的聚合 HK1220733A1 (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US11/583606 2006-10-18
US11/583,606 US7745125B2 (en) 2004-06-28 2006-10-18 2′-terminator related pyrophosphorolysis activated polymerization

Publications (1)

Publication Number Publication Date
HK1220733A1 true HK1220733A1 (zh) 2017-05-12

Family

ID=39283915

Family Applications (1)

Application Number Title Priority Date Filing Date
HK16108797.0A HK1220733A1 (zh) 2006-10-18 2016-07-22 2’-终止子有关的焦磷酸解作用激活的聚合

Country Status (9)

Country Link
US (1) US7745125B2 (enExample)
EP (1) EP2076606B1 (enExample)
JP (1) JP5421112B2 (enExample)
CN (2) CN101528945A (enExample)
AT (1) ATE542922T1 (enExample)
CA (1) CA2664229C (enExample)
ES (1) ES2381505T3 (enExample)
HK (1) HK1220733A1 (enExample)
WO (1) WO2008046602A2 (enExample)

Families Citing this family (38)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7399590B2 (en) 2002-02-21 2008-07-15 Asm Scientific, Inc. Recombinase polymerase amplification
DK1499738T3 (da) 2002-02-21 2008-11-17 Asm Scient Inc Recombinase-polymerease-amplifikation
US8030000B2 (en) 2002-02-21 2011-10-04 Alere San Diego, Inc. Recombinase polymerase amplification
US7572581B2 (en) * 2003-06-30 2009-08-11 Roche Molecular Systems, Inc. 2′-terminator nucleotide-related methods and systems
US7947817B2 (en) * 2003-06-30 2011-05-24 Roche Molecular Systems, Inc. Synthesis and compositions of 2'-terminator nucleotides
US7745125B2 (en) * 2004-06-28 2010-06-29 Roche Molecular Systems, Inc. 2′-terminator related pyrophosphorolysis activated polymerization
US7928207B2 (en) * 2004-06-28 2011-04-19 Roche Molecular Systems, Inc Synthesis and compositions of nucleic acids comprising 2′-terminator nucleotides
ES2420831T3 (es) 2005-07-25 2013-08-27 Alere San Diego, Inc. Procedimientos para multiplexar la ampliación de la recombinasa polimerasa
WO2007075967A2 (en) * 2005-12-21 2007-07-05 Roche Diagnostics Gmbh Sequencing and genotyping using reversibly 2'-modified nucleotides
US8071308B2 (en) 2006-05-04 2011-12-06 Alere San Diego, Inc. Recombinase polymerase amplification
US8962293B2 (en) 2006-10-18 2015-02-24 Roche Molecular Systems, Inc. DNA polymerases and related methods
JP5596554B2 (ja) * 2007-11-28 2014-09-24 エフ．ホフマン−ラ ロシュ アーゲー 向上したピロリン酸分解活性化重合（ｐａｐ）能力を有する変異ｄｎａポリメラーゼ
US10150990B2 (en) 2008-04-21 2018-12-11 Roche Molecular Systems, Inc. Ribonucleotide tag nucleic acid detection
JP5847076B2 (ja) 2009-05-20 2016-01-20 バイオサイト インコーポレイテッド Ｄｎａグリコシラーゼ／リアーゼおよびａｐエンドヌクレアーゼ基質
ES3022638T3 (en) 2009-06-05 2025-05-28 Abbott Diagnostics Scarborough Inc Recombinase polymerase amplification reagents
NZ598166A (en) * 2009-08-11 2014-03-28 Response Genetics Inc Methods, primers, probes and kits useful for the detection of braf mutations
US8409802B2 (en) 2009-08-14 2013-04-02 Roche Molecular Systems, Inc. Format of probes to detect nucleic acid differences
EP2652152A4 (en) * 2010-12-13 2014-06-25 Life Technologies Corp POLYMERIZATION OF NUCLEIC ACIDS BY ACTIVATION BY POLYPHOSPHOROLYSIS REACTIONS (APP)
JP5961247B2 (ja) 2011-04-07 2016-08-02 アリーア サン ディエゴ， インコーポレイテッド リコンビナーゼポリメラーゼ増幅混合物のモニタリング
CN110016499B (zh) 2011-04-15 2023-11-14 约翰·霍普金斯大学 安全测序系统
GEP201706656B (en) 2011-11-11 2017-04-25 Pfizer 2-thiopyrimidinones
KR101545848B1 (ko) * 2012-04-09 2015-08-21 (주)바이오니아 핵산중합효소로 핵산을 검출하는데 사용되는 고민감도 핵산준비방법
PL2912468T3 (pl) 2012-10-29 2019-04-30 Univ Johns Hopkins Test papanicolaou pod kątem raka jajnika i endometrium
US11384377B2 (en) 2013-04-02 2022-07-12 Molecular Assemblies, Inc. Reusable initiators for synthesizing nucleic acids
US9771613B2 (en) 2013-04-02 2017-09-26 Molecular Assemblies, Inc. Methods and apparatus for synthesizing nucleic acid
US10683536B2 (en) 2013-04-02 2020-06-16 Molecular Assemblies, Inc. Reusable initiators for synthesizing nucleic acids
US11331643B2 (en) 2013-04-02 2022-05-17 Molecular Assemblies, Inc. Reusable initiators for synthesizing nucleic acids
US9279149B2 (en) 2013-04-02 2016-03-08 Molecular Assemblies, Inc. Methods and apparatus for synthesizing nucleic acids
US8808989B1 (en) * 2013-04-02 2014-08-19 Molecular Assemblies, Inc. Methods and apparatus for synthesizing nucleic acids
WO2016178113A1 (en) 2015-05-05 2016-11-10 Pfizer Inc. 2-thiopyrimidinones
CN104862401B (zh) * 2015-05-29 2017-10-31 沈阳优吉诺生物科技有限公司 检测kras基因热点突变位点的引物、试剂盒及其pcr方法
WO2017027653A1 (en) 2015-08-11 2017-02-16 The Johns Hopkins University Assaying ovarian cyst fluid
BR112020002555A2 (pt) 2017-08-07 2020-08-11 The Johns Hopkins University métodos e materiais para avaliar e tratar câncer
WO2020016590A1 (en) 2018-07-19 2020-01-23 Biofidelity Ltd Improved polynucleotide sequence detection method
EP4004230A4 (en) * 2019-07-31 2023-08-09 Bioskryb Genomics, Inc. GENE MUTATION ANALYSIS
GB201919186D0 (en) * 2019-12-23 2020-02-05 Biofidelity Ltd Simplified polynucleotide sequence detection method
CA3170345A1 (en) 2020-02-14 2021-08-19 The Johns Hopkins University Methods and materials for assessing nucleic acids
JP7760607B2 (ja) * 2021-04-15 2025-10-27 バイオフィデリティー 核酸の濃縮及び検出

Family Cites Families (59)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NL41678C (enExample) 1931-07-27
US3338882A (en) * 1965-06-28 1967-08-29 Upjohn Co 2'-and 3'-phosphates of 1-beta-d-arabinofuranosylcytosine
JPS5290693A (en) 1977-02-21 1977-07-30 Kyowa Hakko Kogyo Co Ltd Preparation of guanine nucleotide by fermentation
US4458066A (en) * 1980-02-29 1984-07-03 University Patents, Inc. Process for preparing polynucleotides
US4469863A (en) * 1980-11-12 1984-09-04 Ts O Paul O P Nonionic nucleic acid alkyl and aryl phosphonates and processes for manufacture and use thereof
CA1219824A (en) 1981-04-17 1987-03-31 David C. Ward Modified nucleotides and methods of preparing and using same
JPS5985298A (ja) * 1982-11-04 1984-05-17 Yamasa Shoyu Co Ltd ３′−デオキシグアノシンの製造法
US5235033A (en) * 1985-03-15 1993-08-10 Anti-Gene Development Group Alpha-morpholino ribonucleoside derivatives and polymers thereof
US5034506A (en) * 1985-03-15 1991-07-23 Anti-Gene Development Group Uncharged morpholino-based polymers having achiral intersubunit linkages
DE3529478A1 (de) * 1985-08-16 1987-02-19 Boehringer Mannheim Gmbh 7-desaza-2'desoxyguanosin-nukleotide, verfahren zu deren herstellung und deren verwendung zur nukleinsaeure-sequenzierung
US5795762A (en) 1986-08-22 1998-08-18 Roche Molecular Systems, Inc. 5' to 3' exonuclease mutations of thermostable DNA polymerases
US5618711A (en) 1986-08-22 1997-04-08 Hoffmann-La Roche Inc. Recombinant expression vectors and purification methods for Thermus thermophilus DNA polymerase
US5374553A (en) 1986-08-22 1994-12-20 Hoffmann-La Roche Inc. DNA encoding a thermostable nucleic acid polymerase enzyme from thermotoga maritima
US5466591A (en) 1986-08-22 1995-11-14 Hoffmann-La Roche Inc. 5' to 3' exonuclease mutations of thermostable DNA polymerases
US4889818A (en) 1986-08-22 1989-12-26 Cetus Corporation Purified thermostable enzyme
US5455170A (en) 1986-08-22 1995-10-03 Hoffmann-La Roche Inc. Mutated thermostable nucleic acid polymerase enzyme from Thermus species Z05
DE3803021A1 (de) 1988-02-02 1989-08-10 Geiger Reinhard Atp-derivate
US5216141A (en) * 1988-06-06 1993-06-01 Benner Steven A Oligonucleotide analogs containing sulfur linkages
JPH0778074B2 (ja) 1989-09-29 1995-08-23 堺化学工業株式会社 ヌクレオシド―２’，５’‐及び３’，５’‐二リン酸の製造方法
ATE190981T1 (de) * 1989-10-24 2000-04-15 Isis Pharmaceuticals Inc 2'-modifizierte nukleotide
US5914396A (en) * 1990-01-11 1999-06-22 Isis Pharmaceuticals, Inc. 2'-O-modified nucleosides and phosphoramidites
US6005087A (en) * 1995-06-06 1999-12-21 Isis Pharmaceuticals, Inc. 2'-modified oligonucleotides
US5660985A (en) * 1990-06-11 1997-08-26 Nexstar Pharmaceuticals, Inc. High affinity nucleic acid ligands containing modified nucleotides
US5602240A (en) 1990-07-27 1997-02-11 Ciba Geigy Ag. Backbone modified oligonucleotide analogs
US5386023A (en) * 1990-07-27 1995-01-31 Isis Pharmaceuticals Backbone modified oligonucleotide analogs and preparation thereof through reductive coupling
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
US5994056A (en) 1991-05-02 1999-11-30 Roche Molecular Systems, Inc. Homogeneous methods for nucleic acid amplification and detection
US5644048A (en) * 1992-01-10 1997-07-01 Isis Pharmaceuticals, Inc. Process for preparing phosphorothioate oligonucleotides
WO1994006815A1 (en) * 1992-09-11 1994-03-31 Isis Pharmaceuticals, Inc. Oligonucleotide and nucleotide amine analogs, methods of synthesis and use
DE69433811T2 (de) * 1993-01-07 2005-06-23 Sequenom, Inc., San Diego Dns - sequenzierung durch massenspektronomie
US5532130A (en) * 1993-07-20 1996-07-02 Dyad Pharmaceutical Corporation Methods and compositions for sequence-specific hybridization of RNA by 2'-5' oligonucleotides
US5798340A (en) * 1993-09-17 1998-08-25 Gilead Sciences, Inc. Nucleotide analogs
US5637684A (en) * 1994-02-23 1997-06-10 Isis Pharmaceuticals, Inc. Phosphoramidate and phosphorothioamidate oligomeric compounds
DE59510742D1 (de) * 1994-04-27 2003-08-14 Novartis Ag Nukleoside und Oligonukleotide mit 2'-Ethergruppen
JPH09316093A (ja) 1996-03-28 1997-12-09 Sankyo Co Ltd ポリリン酸誘導体
CZ293215B6 (cs) * 1996-08-06 2004-03-17 F. Hoffmann-La Roche Ag Rekombinantní tepelně stálá DNA polymeráza, způsob její přípravy a prostředek, který ji obsahuje
US6699668B1 (en) * 1997-01-15 2004-03-02 Xzillion Gmbh & Co. Mass label linked hybridisation probes
GB9718921D0 (en) 1997-09-05 1997-11-12 Brax Genomics Ltd Catalytically generated mass labels
US20030083273A1 (en) 1999-04-20 2003-05-01 Tod M. Woolf Antisense oligomers
US6147200A (en) * 1999-08-19 2000-11-14 Isis Pharmaceuticals, Inc. 2'-O-acetamido modified monomers and oligomers
EP1259646B1 (en) 2000-02-23 2009-09-02 City of Hope Pyrophosphorolysis activated polymerization (pap): application to allele-specific amplification and nucleic acid sequence determination
US7033763B2 (en) * 2000-02-23 2006-04-25 City Of Hope Pyrophosphorolysis activated polymerization (PAP)
US7179590B2 (en) 2000-04-18 2007-02-20 Roche Molecular Systems, Inc High temperature reverse transcription using mutant DNA polymerases
MY164523A (en) 2000-05-23 2017-12-29 Univ Degli Studi Cagliari Methods and compositions for treating hepatitis c virus
FR2810322B1 (fr) * 2000-06-14 2006-11-10 Pasteur Institut PRODUCTION COMBINATOIRE D'ANALOGUES DE NUCLEOTIDES ET NUCLEOTIDES (XiTP)
SK286630B6 (sk) 2001-01-22 2009-02-05 Merck & Co., Inc. Nukleozidové deriváty, farmaceutický prostriedok s ich obsahom a ich použitie
US6921812B1 (en) * 2001-07-03 2005-07-26 Isis Pharmaceuticals, Inc. Methods of modulating pharmacokinetics of oligonucleotides
EP1409731A2 (en) 2001-07-03 2004-04-21 ISIS Pharmaceuticals, Inc. Methods of modulating pharmacokinetics of oligonucleotides
US7148049B2 (en) 2002-04-02 2006-12-12 Roche Molecular Systems, Inc. Thermostable or thermoactive DNA polymerase molecules with attenuated 3′-5′ exonuclease activity
AU2003241401B8 (en) * 2002-05-10 2009-08-06 City Of Hope Pyrophosphorolysis activated polymerization (PAP)
JP3827629B2 (ja) 2002-08-30 2006-09-27 住友大阪セメント株式会社 光変調器
JP4898119B2 (ja) 2002-11-22 2012-03-14 エフ．ホフマン−ラ ロシュ アーゲー 検出できるラベルされたヌクレオシド類似体及びその使用方法
US7947817B2 (en) 2003-06-30 2011-05-24 Roche Molecular Systems, Inc. Synthesis and compositions of 2'-terminator nucleotides
US7572581B2 (en) 2003-06-30 2009-08-11 Roche Molecular Systems, Inc. 2′-terminator nucleotide-related methods and systems
EP1753774B1 (en) 2004-06-02 2008-09-10 ASM Scientific, Inc. 2"-nitrobenzyl-modified ribonucleotides
US7745125B2 (en) 2004-06-28 2010-06-29 Roche Molecular Systems, Inc. 2′-terminator related pyrophosphorolysis activated polymerization
JP4753066B2 (ja) 2005-06-29 2011-08-17 独立行政法人産業技術総合研究所 イオン複合型粘土組成物およびその固化物
ATE519863T1 (de) 2005-10-05 2011-08-15 Hoffmann La Roche Nicht-fluoreszente energieübertragung
WO2007075967A2 (en) 2005-12-21 2007-07-05 Roche Diagnostics Gmbh Sequencing and genotyping using reversibly 2'-modified nucleotides

Also Published As

Publication number Publication date
JP5421112B2 (ja) 2014-02-19
JP2010506573A (ja) 2010-03-04
CA2664229A1 (en) 2008-04-24
WO2008046602A2 (en) 2008-04-24
CN105274169A (zh) 2016-01-27
ATE542922T1 (de) 2012-02-15
US7745125B2 (en) 2010-06-29
CA2664229C (en) 2012-12-18
EP2076606A2 (en) 2009-07-08
WO2008046602A3 (en) 2008-06-26
EP2076606B1 (en) 2012-01-25
ES2381505T3 (es) 2012-05-28
CN101528945A (zh) 2009-09-09
US20070154914A1 (en) 2007-07-05

Similar Documents

Publication Publication Date Title
EP2076606B1 (en) 2'-terminator related pyrophosphorolysis activated polymerization
CA2740913C (en) Improved allele-specific amplification
CN102119226B (zh) 校对引物延伸
EP2195327A2 (en) Synthesis and compositions of nucleic acids comprising 2'-terminator nucleotides
CA2858209C (en) Methods and reagents for reducing non-specific amplification
CN104838015B (zh) 在等位基因特异性pcr中具有经修饰的磷酸酯和碱基的引物
JP5483403B2 (ja) リボヌクレオチド標識核酸検出
EP3234180A1 (en) Allele-specific amplification of nucleic acids using blocking oligonucleotides for wild type suppression
EP2510118B1 (en) Preferential amplification of mrna over dna using chemically modified primers
HK1136606A (en) 2'-terminator related pyrophosphorolysis activated polymerization
HK1154388A (en) Synthesis and compositions of nucleic acids comprising 2'-terminator nucleotides
HK1159192B (en) Proofreading primer extension
HK1141559B (en) Ribonucleotide tag nucleic acid detection