HK1217536A1 - 種測定蛋白純化工藝過程中樣品的糖基化和末端修飾情況的方法 - Google Patents

種測定蛋白純化工藝過程中樣品的糖基化和末端修飾情況的方法

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Publication number
HK1217536A1
HK1217536A1 HK16105427.4A HK16105427A HK1217536A1 HK 1217536 A1 HK1217536 A1 HK 1217536A1 HK 16105427 A HK16105427 A HK 16105427A HK 1217536 A1 HK1217536 A1 HK 1217536A1
Authority
HK
Hong Kong
Prior art keywords
purification process
protein purification
states during
terminal modification
during protein
Prior art date
Application number
HK16105427.4A
Other languages
English (en)
Inventor
朱保國
彭育才
楊嘉明
Original Assignee
Livzon Mabpharm Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Livzon Mabpharm Inc filed Critical Livzon Mabpharm Inc
Publication of HK1217536A1 publication Critical patent/HK1217536A1/zh

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6848—Methods of protein analysis involving mass spectrometry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6854—Immunoglobulins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/04—Preparation or injection of sample to be analysed
    • G01N30/06—Preparation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/62—Detectors specially adapted therefor
    • G01N30/72—Mass spectrometers
    • G01N30/7233—Mass spectrometers interfaced to liquid or supercritical fluid chromatograph
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N2030/022—Column chromatography characterised by the kind of separation mechanism
    • G01N2030/027—Liquid chromatography
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/04—Preparation or injection of sample to be analysed
    • G01N30/06—Preparation
    • G01N2030/065—Preparation using different phases to separate parts of sample
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/04—Preparation or injection of sample to be analysed
    • G01N30/06—Preparation
    • G01N2030/067—Preparation by reaction, e.g. derivatising the sample
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
    • G01N2030/8809—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample
    • G01N2030/8813—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials
    • G01N2030/8831—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials involving peptides or proteins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2440/00—Post-translational modifications [PTMs] in chemical analysis of biological material
    • G01N2440/38—Post-translational modifications [PTMs] in chemical analysis of biological material addition of carbohydrates, e.g. glycosylation, glycation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2560/00—Chemical aspects of mass spectrometric analysis of biological material
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/24—Nuclear magnetic resonance, electron spin resonance or other spin effects or mass spectrometry

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Biophysics (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Peptides Or Proteins (AREA)
HK16105427.4A 2013-01-15 2016-05-12 種測定蛋白純化工藝過程中樣品的糖基化和末端修飾情況的方法 HK1217536A1 (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CN201310027640.7A CN103217489B (zh) 2013-01-15 2013-01-15 一种测定蛋白纯化工艺过程中样品的糖基化和末端修饰情况的方法
PCT/CN2013/074596 WO2014110874A1 (zh) 2013-01-15 2013-04-24 一种测定蛋白纯化工艺过程中样品的糖基化和末端修饰情况的方法

Publications (1)

Publication Number Publication Date
HK1217536A1 true HK1217536A1 (zh) 2017-01-13

Family

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Family Applications (1)

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HK16105427.4A HK1217536A1 (zh) 2013-01-15 2016-05-12 種測定蛋白純化工藝過程中樣品的糖基化和末端修飾情況的方法

Country Status (6)

Country Link
US (1) US9645156B2 (zh)
EP (1) EP2947454A4 (zh)
JP (1) JP6027258B2 (zh)
CN (1) CN103217489B (zh)
HK (1) HK1217536A1 (zh)
WO (1) WO2014110874A1 (zh)

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ES2861600T3 (es) 2014-04-04 2021-10-06 Mayo Found Medical Education & Res Isotipaje de inmunoglobulinas usando masa molecular precisa
AU2016326757B2 (en) * 2015-09-24 2022-09-01 Mayo Foundation For Medical Education And Research Identification of immunoglobulin free light chains by mass spectrometry
EP3523647B1 (en) 2016-09-07 2024-06-26 Mayo Foundation for Medical Education and Research Identification and monitoring of cleaved immunoglobulins by molecular mass
JOP20190100A1 (ar) * 2016-11-19 2019-05-01 Potenza Therapeutics Inc بروتينات ربط مولد ضد مضاد لـ gitr وطرق استخدامها
CN108333261A (zh) * 2017-01-20 2018-07-27 湖北生物医药产业技术研究院有限公司 检测蛋白电荷变异体的方法及确定生物制品生产工艺的方法
CN108956789A (zh) * 2017-05-17 2018-12-07 中国科学院大连化学物理研究所 一种血清中免疫球蛋白g糖肽的绝对定量分析方法
US11946937B2 (en) 2017-09-13 2024-04-02 Mayo Foundation For Medical Education And Research Identification and monitoring of apoptosis inhibitor of macrophage
WO2019197983A1 (en) * 2018-04-10 2019-10-17 Dh Technologies Development Pte. Ltd. Top-down analysis of antibodies in mass spectrometry
CN110865129B (zh) * 2018-08-27 2022-09-23 齐鲁制药有限公司 一种度拉鲁肽中多种修饰水平的检测方法
CN109932444B (zh) * 2019-03-19 2022-03-11 北京泰德制药股份有限公司 一种糖蛋白多种电荷异构体翻译后修饰的评价方法
CN110609078B (zh) * 2019-09-20 2022-03-11 南京谱利健生物技术有限公司 一种检测蛋白磷酸化和乙酰氨基葡萄糖化关联作用的方法
CN114740108B (zh) * 2022-03-28 2023-07-14 天津键凯科技有限公司 一种聚合物修饰抗体类药物的修饰度的测定方法

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DK2256134T3 (en) * 2003-11-13 2014-02-24 Hanmi Science Co Ltd IgG Fc fragment to a drug carrier and process for preparation thereof
WO2005073732A2 (en) * 2004-01-23 2005-08-11 Amgen Inc. Lc/ms method of analyzing high molecular weight proteins
AU2005216847B2 (en) * 2004-02-27 2010-04-01 Cytiva Bioprocess R&D Ab A process for the purification of antibodies
CN101177453B (zh) 2006-11-07 2010-09-29 旭华（上海）生物研发中心有限公司 抗人肿瘤坏死因子α的重组嵌合抗体
US7820399B2 (en) * 2007-06-01 2010-10-26 Medarex, Inc. Methods for characterizing glycosylation sites
US20090069549A1 (en) * 2007-09-12 2009-03-12 Elias Georges Protein Extraction buffer, a kit comprising it and method of its use
AU2008328370A1 (en) 2007-11-22 2009-05-28 Symphogen A/S A method for characterization of a recombinant polyclonal protein
WO2009080278A1 (en) * 2007-12-21 2009-07-02 F. Hoffmann-La Roche Ag Stability testing of antibodies
SG173589A1 (en) * 2009-02-09 2011-09-29 Roche Glycart Ag Immunoglobulin glycosylation pattern analysis
WO2010141249A2 (en) * 2009-06-02 2010-12-09 Merck Sharp & Dohme Corp. Generation, characterization and uses thereof of anti-notch3 antibodies
AU2010291498A1 (en) 2009-09-07 2012-02-02 F. Hoffmann-La Roche Ag ES-MS of glycopeptides for analysis of glycosylation
CN102675460B (zh) 2011-02-28 2015-08-19 珠海市丽珠单抗生物技术有限公司 抗肿瘤坏死因子α的人源化抗体

Also Published As

Publication number Publication date
US9645156B2 (en) 2017-05-09
WO2014110874A1 (zh) 2014-07-24
EP2947454A4 (en) 2016-08-03
EP2947454A1 (en) 2015-11-25
US20150362506A1 (en) 2015-12-17
JP2016502085A (ja) 2016-01-21
JP6027258B2 (ja) 2016-11-16
CN103217489B (zh) 2016-03-09
CN103217489A (zh) 2013-07-24

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