HK1204340A1 - Protein and gene biomarkers for rejection of organ transplants - Google Patents

Protein and gene biomarkers for rejection of organ transplants

Info

Publication number
HK1204340A1
HK1204340A1 HK15104780.9A HK15104780A HK1204340A1 HK 1204340 A1 HK1204340 A1 HK 1204340A1 HK 15104780 A HK15104780 A HK 15104780A HK 1204340 A1 HK1204340 A1 HK 1204340A1
Authority
HK
Hong Kong
Prior art keywords
rejection
protein
organ transplants
gene biomarkers
biomarkers
Prior art date
Application number
HK15104780.9A
Other languages
English (en)
Chinese (zh)
Inventor
Minnie M Sarwal
Li Li
Tara Sigdel
Amit Kaushal
Wenzhong Xiao
Atul J Butte
Purvesh Khatri
Original Assignee
Univ Leland Stanford Junior
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Leland Stanford Junior filed Critical Univ Leland Stanford Junior
Publication of HK1204340A1 publication Critical patent/HK1204340A1/xx

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6893—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids related to diseases not provided for elsewhere
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2800/00—Detection or diagnosis of diseases
    • G01N2800/24—Immunology or allergic disorders
    • G01N2800/245—Transplantation related diseases, e.g. graft versus host disease

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Genetics & Genomics (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Pathology (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
HK15104780.9A 2010-03-25 2015-05-19 Protein and gene biomarkers for rejection of organ transplants HK1204340A1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US34107110P 2010-03-25 2010-03-25
US201161452288P 2011-03-14 2011-03-14

Publications (1)

Publication Number Publication Date
HK1204340A1 true HK1204340A1 (en) 2015-11-13

Family

ID=44673660

Family Applications (1)

Application Number Title Priority Date Filing Date
HK15104780.9A HK1204340A1 (en) 2010-03-25 2015-05-19 Protein and gene biomarkers for rejection of organ transplants

Country Status (6)

Country Link
US (3) US9535075B2 (xx)
EP (2) EP2803735B1 (xx)
CA (1) CA2794255C (xx)
ES (1) ES2798114T3 (xx)
HK (1) HK1204340A1 (xx)
WO (1) WO2011119980A1 (xx)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2767616A1 (en) 2009-07-09 2011-01-13 The Scripps Research Institute Gene expression profiles associated with chronic allograft nephropathy
CN102854306B (zh) * 2011-12-23 2013-08-07 中国人民解放军第三〇九医院 用于预警肾移植后排斥反应的试剂盒及其使用方法
CN102854323B (zh) * 2011-12-23 2013-08-07 中国人民解放军第三〇九医院 移植肾排斥反应早期诊断试剂盒及其使用方法
CN102854305B (zh) * 2011-12-23 2013-08-07 中国人民解放军第三〇九医院 移植肾排斥反应预警试剂盒及其使用方法
WO2013187556A1 (en) * 2012-06-14 2013-12-19 Scripps Korea Antibody Institute Novel antibody specific for clec14a and uses thereof
EP2698636A1 (en) 2012-08-13 2014-02-19 Fundació Institut d'Investigació Biomèdica de Bellvitge Methods and reagents for prevention and/or treatment of transplant rejection
GB2525568B (en) 2013-03-15 2020-10-14 Abvitro Llc Single cell barcoding for antibody discovery
US20160376652A1 (en) * 2013-09-06 2016-12-29 Immucor Gti Diagnostics, Inc. Compositions and methods for diagnosis and prediction of solid organ graft rejection
US20160348174A1 (en) * 2013-09-06 2016-12-01 Immucor Gti Diagnostics, Inc. Compositions and methods for assessing acute rejection in renal transplantation
CN103698508B (zh) * 2014-01-08 2016-05-18 陈娟 一种结缔组织病早期肺间质病变诊断试剂盒
WO2015138803A1 (en) * 2014-03-12 2015-09-17 Icahn School Of Medicine At Mount Sinai Method for identifying kidney allograft recipients at risk for chronic injury
US11104951B2 (en) 2014-05-22 2021-08-31 The Scripps Research Institute Molecular signatures for distinguishing liver transplant rejections or injuries
AU2015263998A1 (en) * 2014-05-22 2016-12-08 Northwestern University Gene expression profiles associated with sub-clinical kidney transplant rejection
US10443100B2 (en) 2014-05-22 2019-10-15 The Scripps Research Institute Gene expression profiles associated with sub-clinical kidney transplant rejection
CN113444783B (zh) 2014-06-26 2024-04-09 西奈山伊坎医学院 通过分析预示性基因集诊断亚临床和临床的急性排异的方法
CN104198691B (zh) * 2014-08-14 2016-08-17 上海睿康生物科技有限公司 一种稳定的α-淀粉酶检测试剂盒
ES2895750T3 (es) 2014-09-15 2022-02-22 Abvitro Llc Secuenciación de alto rendimiento de colecciones de nucleótidos
WO2016075232A1 (en) * 2014-11-12 2016-05-19 Institut National De La Sante Et De La Recherche Medicale Gene signature associated with tolerance to renal allograft
US20190250147A1 (en) * 2015-10-13 2019-08-15 The Trustees Of The University Of Pennsylvania Methods for using enriched exosomes as a platform for monitoring organ status
WO2017100259A1 (en) * 2015-12-08 2017-06-15 Icahn School Of Medicine At Mount Sinai Pretransplant prediction of post-transplant acute rejection
WO2019107673A1 (ko) * 2017-11-29 2019-06-06 서울대학교병원 Abo 혈액형 부적합 이식시의 항체-매개성 거부반응의 모니터링을 위한 바이오마커
CN112236137A (zh) 2018-04-16 2021-01-15 西奈山伊坎医学院 利用移植前接受者血液中转录组标志预测急性排斥反应和肾脏同种异体移植物丧失的方法和试剂盒
JP2022531660A (ja) * 2019-05-06 2022-07-08 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア 移植拒絶反応の非ｈｌａマーカー
EP4177608A1 (en) 2021-11-05 2023-05-10 Medizinische Hochschule Hannover Biomarker panel for diagnosing pulmonary dysfunction
WO2024068521A1 (en) * 2022-09-26 2024-04-04 Fundación Para La Formación E Investigación Sanitaria De La Región De Murcia In vitro method for predicting organ transplant rejection

Family Cites Families (60)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4591570A (en) 1983-02-02 1986-05-27 Centocor, Inc. Matrix of antibody-coated spots for determination of antigens
US6882301B2 (en) 1986-06-03 2005-04-19 Time Domain Corporation Time domain radio transmission system
US5143854A (en) 1989-06-07 1992-09-01 Affymax Technologies N.V. Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof
JP2000504575A (ja) 1996-02-08 2000-04-18 アフィメトリックス，インコーポレイテッド 微生物のチップベースの種分化および表現型特徴付け
DE69823206T2 (de) 1997-07-25 2004-08-19 Affymetrix, Inc. (a Delaware Corp.), Santa Clara Verfahren zur herstellung einer bio-informatik-datenbank
US20030104371A1 (en) 1997-09-24 2003-06-05 Strom Terry B. Methods of evaluating transplant rejection
DK1032662T3 (da) 1997-11-07 2006-07-03 Trillium Therapeutics Inc Fremgangsmåder og sammensætninger til immunmodulation
US6607879B1 (en) 1998-02-09 2003-08-19 Incyte Corporation Compositions for the detection of blood cell and immunological response gene expression
US6235311B1 (en) 1998-03-18 2001-05-22 Bristol-Myers Squibb Company Pharmaceutical composition containing a combination of a statin and aspirin and method
ATE396399T1 (de) 1998-03-31 2008-06-15 Asahi Kasei Pharma Corp Verfahren zur untersuchung der iga-nephropathie
US20050025769A1 (en) * 1999-01-22 2005-02-03 Fujisawa Pharmaceutical Company, Ltd. Method of suppressing ongoing acute allograft rejection
US7354721B2 (en) 2000-09-22 2008-04-08 Clontech Laboratories, Inc. Highly sensitive proteomic analysis methods, and kits and systems for practicing the same
CA2436139A1 (en) 2001-01-26 2002-08-01 Emory University Methods of inducing organ transplant tolerance and correcting hemoglobinopathies
US20030113744A1 (en) 2001-05-11 2003-06-19 O' Toole Margot M. Methods for diagnosing and treating ischemia and reperfusion injury and compositions thereof
US7235358B2 (en) 2001-06-08 2007-06-26 Expression Diagnostics, Inc. Methods and compositions for diagnosing and monitoring transplant rejection
US7026121B1 (en) 2001-06-08 2006-04-11 Expression Diagnostics, Inc. Methods and compositions for diagnosing and monitoring transplant rejection
AU2002318250A1 (en) 2001-07-13 2003-01-29 University Of Pittsburgh-Of The Commonwealth System Of Higher Education Method for identifying tolerance in graft recipients
US20070134261A1 (en) 2001-12-03 2007-06-14 Hancock Robert E Effectors of innate immunity
PT1567138E (pt) 2002-11-21 2011-04-11 Genzyme Corp Utilização de derivados de diamida para inibição de rejeição crónica de transplante
CA2516013C (en) 2003-02-14 2014-05-13 Beth Israel Deaconess Medical Center, Inc. Predicting graft rejection
US7892745B2 (en) 2003-04-24 2011-02-22 Xdx, Inc. Methods and compositions for diagnosing and monitoring transplant rejection
CA2528669A1 (en) 2003-06-09 2005-01-20 The Regents Of The University Of Michigan Compositions and methods for treating and diagnosing cancer
US7648825B2 (en) 2003-06-20 2010-01-19 University Of Florida Research Foundation, Inc. Biomarkers for differentiating between type 1 and type 2 diabetes
WO2005054503A2 (en) 2003-12-03 2005-06-16 Novartis Ag Biomarkers for graft rejection
US8932808B1 (en) 2004-01-21 2015-01-13 The Board Of Trustees Of The Leland Stanford Junior University Methods and compositions for determining a graft tolerant phenotype in a subject
WO2005074540A2 (en) 2004-01-30 2005-08-18 University Of Pennsylvania Novel predictors of transplant rejection determined by peripheral blood gene-expression profiling
WO2006033664A1 (en) 2004-03-08 2006-03-30 Avalon Pharmaceuticals Determining cancer-linked genes and therapeutic targets using molecular cytogenetic methods
US20070232658A1 (en) 2004-04-08 2007-10-04 Jurgen Wagner Protein Kinase C Inhibitors for the Treatment of Autoimmune Diseases and of Transplant Rejiction
EP1784509A2 (en) 2004-07-20 2007-05-16 Wyeth a Corporation of the State of Delaware Methods of identifying patients at risk of developing encephalitis following immunotherapy for alzheimer's disease
US7820382B2 (en) 2004-08-03 2010-10-26 Bauer A Robert Method for the early detection of breast cancer, lung cancer, pancreatic cancer and colon polyps, growths and cancers as well as other gastrointestinal disease conditions and the preoperative and postoperative monitoring of transplanted organs from the donor and in the recipient and their associated conditions related and unrelated to the organ transplantation
US20080280282A1 (en) 2004-08-03 2008-11-13 Bauer Jr A Robert Method for early detection of various cancers and gastrointestinal disease and monitoring of transplanted organs
GB0502042D0 (en) 2005-02-01 2005-03-09 Univ Glasgow Materials and methods for diagnosis and treatment of chronic fatigue syndrome
WO2006099421A2 (en) * 2005-03-14 2006-09-21 The Board Of Trustees Of The Leland Stanford Junior University Methods and compositions for evaluating graft survival in a solid organ transplant recipient
US7666596B2 (en) 2005-05-23 2010-02-23 University Of Alberta Tissue rejection
EP1909810B2 (en) 2005-06-07 2017-08-23 The Regents of the University of Colorado Inhibitors of serine protease activity and their use in methods and compositions for treatment of graft rejection and promotion of graft survival
EP1731620A1 (en) 2005-06-07 2006-12-13 Institut National De La Sante Et De La Recherche Medicale (Inserm) Method for the diagnosis of immune graft tolerance
JP5904569B2 (ja) 2005-06-08 2016-04-13 ミレニアム ファーマシューティカルズ， インコーポレイテッドＭｉｌｌｅｎｎｉｕｍ Ｐｈａｒｍａｃｅｕｔｉｃａｌｓ， Ｉｎｃ． 癌治療を受けている患者の同定、判定および処置のための方法
WO2007050588A2 (en) 2005-10-25 2007-05-03 Renovar Incorporated Systems and methods for characterizing conditions of organs for transplantation
CA2641079A1 (en) 2006-02-06 2007-08-16 Rappaport Family Institute For Research In The Medical Sciences Methods and kit for diagnosing t1dm
GB0605217D0 (en) * 2006-03-15 2006-04-26 Novartis Ag Method and compositions for assessing acute rejection
WO2007112097A2 (en) 2006-03-24 2007-10-04 Children's Medical Center Corporation Novel signature self renewal gene expression programs
GB0607943D0 (en) 2006-04-21 2006-05-31 Novartis Ag Biomarkers for chronic transplant dysfunction
CA2650507A1 (en) 2006-04-27 2007-11-08 Universite De Montreal Assessment and reduction of risk of graft-versus-host disease
WO2008084331A2 (en) 2006-06-21 2008-07-17 Hopitaux Universitaires De Geneve Biomarkers for renal disorders
US20090176656A1 (en) 2006-07-21 2009-07-09 Halloran Philip F Tissue rejection
CA2661487A1 (en) 2006-08-28 2008-03-06 Source Precision Medicine Gene expression profiling for identification, monitoring and treatment of transplant rejection
EP2506172A1 (en) 2006-11-03 2012-10-03 Baylor Research Institute Diagnosis of metastatic melanoma and monitoring indicators of immunosuppression through blood leukocyte microarray analysis
ES2324751B1 (es) 2007-01-04 2010-05-31 Institut D'investigacions Biomediques August Pi I Sunyer (Idibaps) Metodos y kits para diagnosticar y/o pronosticar el estado de tolerancia en el trasplante de higado.
WO2008091814A2 (en) 2007-01-22 2008-07-31 Wyeth Assessment of asthma and allergen-dependent gene expression
EP1958637A1 (en) 2007-02-14 2008-08-20 Revotar Biopharmaceuticals AG Pharmaceutical composition for the treatment of IL-8 mediated diseases
US20100234292A1 (en) 2007-04-16 2010-09-16 Ipsogen Methods of assessing a propensity of clinical outcome for a female mammal suffering from breast cancer
JPWO2009044899A1 (ja) 2007-10-03 2011-02-17 協和発酵キリン株式会社 細胞の増殖を制御する核酸
EP2283153A4 (en) 2008-04-09 2011-09-14 Univ British Columbia METHODS OF DIAGNOSING THE ACUTE REJECTION OF A CARDIAC ALLOGRAFT
WO2009143624A1 (en) 2008-05-30 2009-12-03 The University Of British Columbia Methods of diagnosing rejection of a kidney allograft using genomic or proteomic expression profiling
CN102124340B (zh) 2008-06-02 2014-12-17 卫材R&D管理有限公司 IgA肾病的检查方法和检查试剂盒
US20110201519A1 (en) 2008-08-18 2011-08-18 Sarwal Minnie M Methods and Compositions for Determining a Graft Tolerant Phenotype in a Subject
KR100928403B1 (ko) 2008-10-01 2009-11-26 경북대학교 산학협력단 티지비엠 신증의 진단용 조성물 및 키트
US9938579B2 (en) * 2009-01-15 2018-04-10 The Board Of Trustees Of The Leland Stanford Junior University Biomarker panel for diagnosis and prediction of graft rejection
US8734786B2 (en) 2009-09-16 2014-05-27 Northwestern University Use of ECDI-fixed cell tolerance as a method for preventing allograft rejection
JP2013528799A (ja) 2010-05-13 2013-07-11 ユニバーシティ オブ メディスン アンド デンティストリー オブ ニュー ジャージー 神経変性疾患の検知および診断のための診断用自己抗体プロファイル

Also Published As

Publication number Publication date
US9535075B2 (en) 2017-01-03
EP2550371A1 (en) 2013-01-30
ES2798114T3 (es) 2020-12-09
US20130143755A1 (en) 2013-06-06
EP2803735A1 (en) 2014-11-19
CA2794255C (en) 2020-01-14
CA2794255A1 (en) 2011-09-29
US20210199669A1 (en) 2021-07-01
EP2550371A4 (en) 2013-11-20
US11768208B2 (en) 2023-09-26
WO2011119980A1 (en) 2011-09-29
EP2803735B1 (en) 2020-03-25
US20170227549A1 (en) 2017-08-10

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