HK1163165A1 - 具有增强的熱穩定性的新穎的 聚合酶變體 - Google Patents

具有增强的熱穩定性的新穎的 聚合酶變體

Info

Publication number
HK1163165A1
HK1163165A1 HK12103705.6A HK12103705A HK1163165A1 HK 1163165 A1 HK1163165 A1 HK 1163165A1 HK 12103705 A HK12103705 A HK 12103705A HK 1163165 A1 HK1163165 A1 HK 1163165A1
Authority
HK
Hong Kong
Prior art keywords
rna
novel
polymerase variants
enhanced thermostability
rna polymerase
Prior art date
Application number
HK12103705.6A
Other languages
English (en)
Inventor
Michael Greif
Rainer Mueller
Christian Rudolph
Armin Ruf
Manfred Schmidt
Harald Sobek
Johann-Peter Thalhofer
Original Assignee
Hoffmann La Roche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche
Publication of HK1163165A1 publication Critical patent/HK1163165A1/zh

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1247—DNA-directed RNA polymerase (2.7.7.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07006—DNA-directed RNA polymerase (2.7.7.6)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
HK12103705.6A 2010-04-16 2012-04-16 具有增强的熱穩定性的新穎的 聚合酶變體 HK1163165A1 (zh)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP10004059 2010-04-16

Publications (1)

Publication Number Publication Date
HK1163165A1 true HK1163165A1 (zh) 2012-09-07

Family

ID=42460013

Family Applications (1)

Application Number Title Priority Date Filing Date
HK12103705.6A HK1163165A1 (zh) 2010-04-16 2012-04-16 具有增强的熱穩定性的新穎的 聚合酶變體

Country Status (6)

Country Link
US (3) US9193959B2 (zh)
EP (1) EP2377928A3 (zh)
JP (1) JP5851709B2 (zh)
CN (1) CN102220294B (zh)
CA (1) CA2737014A1 (zh)
HK (1) HK1163165A1 (zh)

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP6280367B2 (ja) 2010-08-31 2018-02-14 グリーンライト バイオサイエンシーズ インコーポレーテッドＧｒｅｅｎｌｉｇｈｔ Ｂｉｏｓｃｉｅｎｃｅｓ，Ｉｎｃ． プロテアーゼ操作を介した代謝経路におけるフラックスの制御のための方法
US9045740B2 (en) * 2012-02-24 2015-06-02 University Of Massachusetts Modified T7-related RNA polymerases and methods of use thereof
AU2014306074B2 (en) 2013-08-05 2018-08-30 Greenlight Biosciences, Inc. Engineered proteins with a protease cleavage site
WO2016160936A1 (en) 2015-03-30 2016-10-06 Greenlight Biosciences, Inc. Cell-free production of ribonucleic acid
CN108779446B (zh) 2016-01-13 2023-04-21 新英格兰生物实验室公司 T7 rna聚合酶的热稳定变体
KR102536687B1 (ko) 2016-04-06 2023-05-25 그린라이트 바이오사이언시스, 아이엔씨. 리보핵산의 무세포 생산
CN107460177B (zh) * 2016-06-06 2021-08-17 张海生 可利用化学修饰核苷酸的rna聚合酶突变体
WO2018111967A1 (en) 2016-12-13 2018-06-21 Modernatx, Inc. Rna affinity purification
US10034951B1 (en) * 2017-06-21 2018-07-31 New England Biolabs, Inc. Use of thermostable RNA polymerases to produce RNAs having reduced immunogenicity
CN111032863A (zh) 2017-06-30 2020-04-17 科德克希思公司 T7 rna聚合酶变体
CN111417724A (zh) 2017-06-30 2020-07-14 科德克希思公司 T7 rna聚合酶变体
EP3668971B8 (en) 2017-08-18 2024-05-29 ModernaTX, Inc. Rna polymerase variants
KR20230130144A (ko) 2017-10-11 2023-09-11 그린라이트 바이오사이언시스, 아이엔씨. 뉴클레오시드 트리포스페이트 및 리보핵산 생산을 위한 방법 및 조성물
EP3768832B1 (en) 2018-03-21 2023-11-29 F. Hoffmann-La Roche AG Dna polymerases for efficient and effective incorporation of methylated-dntps
AU2019253693A1 (en) * 2018-04-10 2020-10-22 Greenlight Biosciences, Inc. T7 RNA polymerase variants
US11851694B1 (en) 2019-02-20 2023-12-26 Modernatx, Inc. High fidelity in vitro transcription
WO2020172239A1 (en) * 2019-02-20 2020-08-27 Modernatx, Inc. Rna polymerase variants for co-transcriptional capping
CA3188462A1 (en) * 2020-08-06 2022-02-10 Ting Zhu Chemical synthesis of large and mirror-image proteins and uses thereof
WO2022084748A1 (en) 2020-10-23 2022-04-28 Spindle Biotech, Inc. Compositions and methods for rna synthesis
CN112980811B (zh) * 2021-03-06 2022-02-25 苏州瀚源新酶生物科技有限公司 Rna聚合酶突变体及其应用、重组载体及其制备方法和应用、重组工程菌及其应用
CN114774453B (zh) * 2022-03-14 2023-04-21 湖北大学 一种运动发酵单胞菌基因表达严谨调控系统的构建方法和应用

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4952496A (en) 1984-03-30 1990-08-28 Associated Universities, Inc. Cloning and expression of the gene for bacteriophage T7 RNA polymerase
US5385834A (en) * 1993-08-13 1995-01-31 Georgia Tech Research Corporation Mutant T7 RNA polymerase GP1(lys222) exhibiting altered promoter recognition
US6524828B1 (en) 1998-12-11 2003-02-25 Akzo Nobel N.V. Mutant of RNA polymerases with increased stability
JP4399684B2 (ja) * 1999-08-17 2010-01-20 東洋紡績株式会社 改良されたｒｎａポリメラーゼ
ES2242733T3 (es) * 2000-03-07 2005-11-16 Biomerieux B.V. Mutante de arn polimerasa con termoestabilidad mejorada.
JP2003061683A (ja) * 2001-06-11 2003-03-04 Nippon Genetech Co Ltd 変異型ｒｎａポリメラーゼ
WO2009113718A1 (ja) 2008-03-11 2009-09-17 東ソー株式会社 機能改善したｒｎａポリメラーゼ変異体
KR20110044753A (ko) * 2008-08-08 2011-04-29 토소가부시키가이샤 기능 개선된 ｒｎａ 중합효소 변이체
JP5789949B2 (ja) * 2010-10-05 2015-10-07 東ソー株式会社 改良されたｒｎａポリメラーゼ変異体

Also Published As

Publication number Publication date
US20160032260A1 (en) 2016-02-04
US20110256589A1 (en) 2011-10-20
US9193959B2 (en) 2015-11-24
EP2377928A2 (en) 2011-10-19
CA2737014A1 (en) 2011-10-16
EP2377928A3 (en) 2012-06-27
JP2011224002A (ja) 2011-11-10
CN102220294A (zh) 2011-10-19
JP5851709B2 (ja) 2016-02-03
CN102220294B (zh) 2014-11-12
US20160032261A1 (en) 2016-02-04

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Legal Events

Date Code Title Description
PC Patent ceased (i.e. patent has lapsed due to the failure to pay the renewal fee)

Effective date: 20190418