HK1161746A1 - Multiplex amplification and detection - Google Patents

Multiplex amplification and detection

Info

Publication number
HK1161746A1
HK1161746A1 HK12101720.1A HK12101720A HK1161746A1 HK 1161746 A1 HK1161746 A1 HK 1161746A1 HK 12101720 A HK12101720 A HK 12101720A HK 1161746 A1 HK1161746 A1 HK 1161746A1
Authority
HK
Hong Kong
Prior art keywords
detection
multiplex amplification
multiplex
amplification
Prior art date
Application number
HK12101720.1A
Other languages
English (en)
Chinese (zh)
Inventor
富國良
Original Assignee
奥西泰克有限公司
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB0814002A external-priority patent/GB0814002D0/en
Priority claimed from GB0817706A external-priority patent/GB0817706D0/en
Priority claimed from GBGB0909333.7A external-priority patent/GB0909333D0/en
Application filed by 奥西泰克有限公司 filed Critical 奥西泰克有限公司
Publication of HK1161746A1 publication Critical patent/HK1161746A1/xx

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6823—Release of bound markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
HK12101720.1A 2008-07-31 2012-02-21 Multiplex amplification and detection HK1161746A1 (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
GB0814002A GB0814002D0 (en) 2008-07-31 2008-07-31 Multiplex amplification and detection
GB0817706A GB0817706D0 (en) 2008-09-26 2008-09-26 Multiplex amplification and detection
GBGB0909333.7A GB0909333D0 (en) 2009-06-01 2009-06-01 Multiplex amplification and detection
PCT/GB2009/001897 WO2010013017A1 (fr) 2008-07-31 2009-07-30 Amplification et détection multiplex

Publications (1)

Publication Number Publication Date
HK1161746A1 true HK1161746A1 (en) 2012-08-03

Family

ID=41050341

Family Applications (1)

Application Number Title Priority Date Filing Date
HK12101720.1A HK1161746A1 (en) 2008-07-31 2012-02-21 Multiplex amplification and detection

Country Status (7)

Country Link
US (2) US9234232B2 (fr)
EP (1) EP2307574B1 (fr)
CN (1) CN102171366B (fr)
CA (1) CA2738792C (fr)
ES (1) ES2396044T3 (fr)
HK (1) HK1161746A1 (fr)
WO (1) WO2010013017A1 (fr)

Families Citing this family (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2582602T3 (es) 2007-03-28 2016-09-14 Signal Diagnostics Sistema y método de análisis de alta resolución de ácidos nucleicos para detectar variaciones de secuencia
US10669574B2 (en) 2008-11-18 2020-06-02 XCR Diagnostics, Inc. DNA amplification technology
WO2016007914A1 (fr) * 2014-07-10 2016-01-14 Fluoresentric, Inc. Technologie d'amplification d'adn
CN101871007B (zh) * 2010-05-07 2014-04-30 无锡锐奇基因生物科技有限公司 用标记探针进行检测和熔解曲线分析的方法
DE102010025161A1 (de) * 2010-06-25 2011-12-29 Qiagen Gmbh Verfahren zum Nachweis von Ribonukleinsäuren
GB201017978D0 (en) * 2010-10-25 2010-12-08 Oxitec Ltd Multiplex amplification and detection
KR20130101952A (ko) 2012-02-02 2013-09-16 주식회사 씨젠 Ｐｔｏ 절단과 연장-의존적 혼성화를 이용한 타겟 핵산서열의 검출
EP2809813B1 (fr) 2012-02-03 2018-08-08 California Institute of Technology Codage ou décodage de signaux dans des dosages biochimiques multiplexés
US9074249B2 (en) 2012-06-04 2015-07-07 New England Biolabs, Inc. Detection of amplification products
US9074243B2 (en) 2012-07-27 2015-07-07 New England Biolabs, Inc. Detection of amplification products
WO2014022827A1 (fr) 2012-08-03 2014-02-06 California Institute Of Technology Multiplexage et quantification dans pcr avec exigences et matériel réduits
US9222122B2 (en) 2012-11-20 2015-12-29 Src, Inc. System and method for rapid detection and identification of nucleic acid labeled tags
US10370707B2 (en) 2013-10-09 2019-08-06 Fluoresentric, Inc. Multiplex probes
WO2015147370A1 (fr) * 2014-03-28 2015-10-01 Seegene, Inc. Détection de séquences d'acide nucléique cible à l'aide de différentes températures de détection
US9982291B2 (en) 2014-08-11 2018-05-29 Luminex Corporation Probes for improved melt discrimination and multiplexing in nucleic acid assays
CN107208139B (zh) 2014-12-09 2020-11-10 Seegene株式会社 与靶核酸序列有关的信号的分辨
US11485998B2 (en) 2015-12-15 2022-11-01 Seegene, Inc. Signal extraction for a target nucleic acid sequence
CN109642252A (zh) * 2016-06-17 2019-04-16 加州理工学院 核酸反应及相关方法和组合物
US11034997B2 (en) * 2016-09-15 2021-06-15 Roche Molecular Systems, Inc. Methods for performing multiplexed real-time PCR
RU2665631C2 (ru) * 2017-01-25 2018-09-03 Общество с ограниченной ответственностью "Секвойя Дженетикс" Способ специфической идентификации последовательностей днк
KR101899371B1 (ko) * 2017-07-25 2018-10-29 (주)엔바이오텍 핵산 복합체 페어, 핵산 복합체 페어를 포함하는 pcr용 키트, 및 핵산 복합체 페어를 이용한 타겟 검출 방법
KR101919896B1 (ko) * 2018-05-03 2018-11-19 주식회사 유디피아 단일 형광 채널 유전자증폭기기를 이용한 다중 핵산 검출 방법
CN112852935B (zh) * 2020-01-30 2022-03-04 上海快灵生物科技有限公司 基于双标记寡核苷酸探针熔解曲线的多重检测靶核苷酸序列的方法及其试剂盒
CN117795093A (zh) * 2021-06-25 2024-03-29 豪夫迈·罗氏有限公司 具有增加的灵敏度的用于进行温度多重pcr的方法
CN116356075A (zh) * 2021-12-27 2023-06-30 迈克生物股份有限公司 一种用于检测的引物探针、引物探针组及其应用
WO2023212628A1 (fr) * 2022-04-27 2023-11-02 Exact Sciences Corporation Dosages d'amplification multiplex séquentielle à biais faible
CN116121241A (zh) * 2023-02-01 2023-05-16 广州臻富科技有限公司 探针、检测试剂盒及靶核酸甲基化的检测方法

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0232967B1 (fr) 1986-01-10 1993-04-28 Amoco Corporation Dosage homogène concomitant
US6140054A (en) 1998-09-30 2000-10-31 University Of Utah Research Foundation Multiplex genotyping using fluorescent hybridization probes
US6623919B1 (en) * 1999-02-03 2003-09-23 Ortho-Clinical Diagnostics, Inc Oligonucleotide primers for efficient multiplex detection of hepatitis C virus (HCV) and human immunodeficiency virus (HIV) and methods of use thereof
US6472156B1 (en) 1999-08-30 2002-10-29 The University Of Utah Homogeneous multiplex hybridization analysis by color and Tm
US20050053950A1 (en) 2003-09-08 2005-03-10 Enrique Zudaire Ubani Protocol and software for multiplex real-time PCR quantification based on the different melting temperatures of amplicons
CA2548205C (fr) 2003-12-03 2017-06-27 Abbott Laboratories Sonde d'acide nucleique lineaire bicatenaire et ses utilisations
AU2005287517A1 (en) * 2004-09-24 2006-03-30 Universitaet Bern Molecular beacons
ES2327956T3 (es) * 2006-10-12 2009-11-05 Bio-Rad Pasteur Sondas de doble hebra para la deteccion de acidos nucleicos, mediante fluorescencia.

Also Published As

Publication number Publication date
EP2307574B1 (fr) 2012-09-19
ES2396044T3 (es) 2013-02-18
CA2738792C (fr) 2016-11-22
CA2738792A1 (fr) 2010-02-04
CN102171366A (zh) 2011-08-31
CN102171366B (zh) 2014-11-26
US20110207131A1 (en) 2011-08-25
US20160115526A1 (en) 2016-04-28
EP2307574A1 (fr) 2011-04-13
WO2010013017A1 (fr) 2010-02-04
US9234232B2 (en) 2016-01-12

Similar Documents

Publication Publication Date Title
HK1161746A1 (en) Multiplex amplification and detection
HK1196358A1 (en) Substituted pyridin-2-ones and pyridazin-3-ones -2--3-
GB2456567B (en) Novelty detection
IL210804A (en) Benzylidenehydrazides and their uses
IL211042A0 (en) Imidazopyridin-2-one derivatives and uses thereof
EP2243036A4 (fr) Systèmes et procédés de détection d'activités
EP2351211A4 (fr) Détection de la saturation d un amplificateur de puissance
ZA201103635B (en) Azaquinolinone derivatives and uses thereof
EP2225360A4 (fr) Dispositifs et procédés de détection
GB0808575D0 (en) Compilign and linking
EP2212992A4 (fr) Dispositif d'amplification amélioré
GB0918787D0 (en) Multiplex amplification and detection
ZA201103456B (en) Leukolectins and uses thereof
EP2291377A4 (fr) Imidazopyrimidinones et leurs utilisations
IL213321A0 (en) Descenting systems and methods
GB0816452D0 (en) Isolation detection
GB201012210D0 (en) Connection methods and systems
EP2255218A4 (fr) Détection d'une entité en mouvement
PL2247601T3 (pl) Tiazopirymidynony i ich zastosowanie
GB201014933D0 (en) Angled-penetrator device and system
GB2465235B (en) Amplifier
GB0813359D0 (en) Halfin and/or platerfin
IL206951A0 (en) 3-alkyl-piperazine derivatives and uses thereof
EP2274294A4 (fr) 5-diméthoxy fumagillol et ses dérivés
GB0817706D0 (en) Multiplex amplification and detection

Legal Events

Date Code Title Description
PC Patent ceased (i.e. patent has lapsed due to the failure to pay the renewal fee)

Effective date: 20220730