HK1119737A1 - Mammalian expression vector comprising the mcmv promoter and first intron of hcmv major immediate early gene - Google Patents

Mammalian expression vector comprising the mcmv promoter and first intron of hcmv major immediate early gene

Info

Publication number
HK1119737A1
HK1119737A1 HK08111454.8A HK08111454A HK1119737A1 HK 1119737 A1 HK1119737 A1 HK 1119737A1 HK 08111454 A HK08111454 A HK 08111454A HK 1119737 A1 HK1119737 A1 HK 1119737A1
Authority
HK
Hong Kong
Prior art keywords
intron
expression vector
mammalian expression
immediate early
early gene
Prior art date
Application number
HK08111454.8A
Other languages
English (en)
Inventor
Robert Gay
Robert Kallmeier
Alison Norman
Stephan Kalwy
Original Assignee
Lonza Biologics Plc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=37045861&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=HK1119737(A1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Priority claimed from GB0508154A external-priority patent/GB0508154D0/en
Priority claimed from GB0508802A external-priority patent/GB0508802D0/en
Application filed by Lonza Biologics Plc filed Critical Lonza Biologics Plc
Publication of HK1119737A1 publication Critical patent/HK1119737A1/xx

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/10—Plasmid DNA
    • C12N2800/108—Plasmid DNA episomal vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/42—Vector systems having a special element relevant for transcription being an intron or intervening sequence for splicing and/or stability of RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
HK08111454.8A 2005-04-22 2008-10-16 Mammalian expression vector comprising the mcmv promoter and first intron of hcmv major immediate early gene HK1119737A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB0508154A GB0508154D0 (en) 2005-04-22 2005-04-22 Mammalian expression vector comprising the mCMV promoter and first intron of hCMV major immediate early gene
GB0508802A GB0508802D0 (en) 2005-04-29 2005-04-29 Mammalian expression vector comprising the mcmv promoter and first intron of hcmv major immediate early gene
PCT/EP2006/003641 WO2006111387A2 (fr) 2005-04-22 2006-04-20 Vecteur d'expression mammalien comprenant le promoteur mcmv et premier intron du gene precoce immediat majeur hcmv

Publications (1)

Publication Number Publication Date
HK1119737A1 true HK1119737A1 (en) 2009-03-13

Family

ID=37045861

Family Applications (1)

Application Number Title Priority Date Filing Date
HK08111454.8A HK1119737A1 (en) 2005-04-22 2008-10-16 Mammalian expression vector comprising the mcmv promoter and first intron of hcmv major immediate early gene

Country Status (8)

Country Link
US (1) US20090181424A1 (fr)
EP (1) EP1874929A2 (fr)
JP (1) JP2008536506A (fr)
KR (1) KR101302904B1 (fr)
AU (1) AU2006237193B2 (fr)
CA (1) CA2600986A1 (fr)
HK (1) HK1119737A1 (fr)
WO (1) WO2006111387A2 (fr)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101273829B1 (ko) 2005-05-20 2013-06-11 론자 바이올로직스 피엘씨 포유류 숙주 세포에서의 재조합 항체의 고-수준 발현
US8883493B2 (en) * 2007-01-30 2014-11-11 Cedars-Sinai Medical Center Adenoviral vector comprising herpes simplex virus type 1 thymidine kinase and a transgene for increasing the expression of the transgene
EP2300600B1 (fr) * 2008-06-13 2015-09-30 Janssen Biotech, Inc. Procédés pour améliorer la viabilité et la productivité dans une culture cellulaire
WO2011027575A1 (fr) 2009-09-04 2011-03-10 独立行政法人医薬基盤研究所 Activateur pour promoteur, et utilisation de celui-ci
ES2540753T3 (es) * 2012-09-24 2015-07-13 Lonza Biologics Plc. Vectores de expresión que comprenden secuencias quiméricas de promotor y amplificador de citomegalovirus
CN105658799A (zh) * 2013-10-07 2016-06-08 普雷斯蒂奇生物制药私人有限公司 用于抗体表达的双顺反子表达载体以及用其生产抗体的方法
KR102244434B1 (ko) * 2014-08-11 2021-04-23 삼성전자주식회사 재조합 벡터 및 이를 이용한 목적 폴리펩타이드의 생산 방법
CN104195173B (zh) * 2014-09-02 2017-03-08 北京比洋生物技术有限公司 具有双表达盒的谷氨酰胺合成酶高效表达载体
KR20160068558A (ko) 2014-12-05 2016-06-15 삼성바이오에피스 주식회사 마우스 cmv 프로모터를 포함하는 융합 폴리뉴클레오타이드 및 이를 이용한 목적 폴리펩타이드의 생산 방법
WO2018146205A1 (fr) * 2017-02-09 2018-08-16 Janssen Vaccines & Prevention B.V. Promoteur puissant et court pour l'expression de gènes hétérologues
CN110785494B (zh) 2017-06-16 2024-07-02 隆萨有限公司 用于生产生物制品的通用自调节哺乳动物细胞系平台
US11566262B2 (en) * 2021-03-24 2023-01-31 Dna Twopointo Inc. Tetracycline-inducible expression systems
WO2023059112A1 (fr) * 2021-10-06 2023-04-13 주식회사 지앤피바이오사이언스 Développement d'une construction d'expression recombinée optimale
CN114958914B (zh) * 2022-06-06 2023-09-26 新乡医学院 一种人类及哺乳动物细胞附着体表达载体、构建方法和应用

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4968615A (en) * 1985-12-18 1990-11-06 Ciba-Geigy Corporation Deoxyribonucleic acid segment from a virus
WO2001002607A1 (fr) * 1999-07-06 2001-01-11 Merck & Co., Inc. Vaccin contre le vih comprenant un adenovirus vehiculant le gene gag
EP1373524B1 (fr) 2000-10-13 2006-04-12 Chiron Corporation Fragments d'intron a de cytomegalovirus
GB0216648D0 (en) * 2002-07-18 2002-08-28 Lonza Biologics Plc Method of expressing recombinant protein in CHO cells
ATE399872T1 (de) 2003-03-11 2008-07-15 Serono Lab Expressionvektoren, die den promotor des ie2-gens des maus cytomegalovirus enthalten

Also Published As

Publication number Publication date
AU2006237193B2 (en) 2012-02-02
WO2006111387A3 (fr) 2007-03-29
CA2600986A1 (fr) 2006-10-26
WO2006111387A2 (fr) 2006-10-26
JP2008536506A (ja) 2008-09-11
KR101302904B1 (ko) 2013-09-06
AU2006237193A1 (en) 2006-10-26
EP1874929A2 (fr) 2008-01-09
US20090181424A1 (en) 2009-07-16
KR20080003384A (ko) 2008-01-07

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