GR3022094T3 - Recombinant DNA expression vectors and DNA compounds that encode deacetoxycephalosporin C synthetase and deacetylcephalosporin C synthetase - Google Patents

Recombinant DNA expression vectors and DNA compounds that encode deacetoxycephalosporin C synthetase and deacetylcephalosporin C synthetase

Info

Publication number
GR3022094T3
GR3022094T3 GR960403542T GR960403542T GR3022094T3 GR 3022094 T3 GR3022094 T3 GR 3022094T3 GR 960403542 T GR960403542 T GR 960403542T GR 960403542 T GR960403542 T GR 960403542T GR 3022094 T3 GR3022094 T3 GR 3022094T3
Authority
GR
Greece
Prior art keywords
synthetase
deacetylcephalosporin
deacetoxycephalosporin
encode
expression vectors
Prior art date
Application number
GR960403542T
Other languages
English (en)
Inventor
Thomas Dominick Ingolia
Stephen Wyatt Queener
Suellen Mary Samson
Paul Luther Skatrud
Original Assignee
Lilly Co Eli
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Lilly Co Eli filed Critical Lilly Co Eli
Publication of GR3022094T3 publication Critical patent/GR3022094T3/el

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Cephalosporin Compounds (AREA)
GR960403542T 1987-03-04 1996-12-18 Recombinant DNA expression vectors and DNA compounds that encode deacetoxycephalosporin C synthetase and deacetylcephalosporin C synthetase GR3022094T3 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US2183687A 1987-03-04 1987-03-04

Publications (1)

Publication Number Publication Date
GR3022094T3 true GR3022094T3 (en) 1997-03-31

Family

ID=21806425

Family Applications (1)

Application Number Title Priority Date Filing Date
GR960403542T GR3022094T3 (en) 1987-03-04 1996-12-18 Recombinant DNA expression vectors and DNA compounds that encode deacetoxycephalosporin C synthetase and deacetylcephalosporin C synthetase

Country Status (17)

Country Link
US (1) US6180361B1 (el)
EP (1) EP0281391B1 (el)
JP (1) JPS63301790A (el)
KR (1) KR960015264B1 (el)
CN (1) CN1026015C (el)
AU (1) AU608572B2 (el)
BG (1) BG50281A3 (el)
CA (1) CA1327170C (el)
DE (1) DE3855633T2 (el)
DK (1) DK102088A (el)
ES (1) ES2095204T3 (el)
FI (1) FI880888A (el)
GR (1) GR3022094T3 (el)
HU (1) HU205387B (el)
IL (1) IL85535A (el)
RU (1) RU1838413C (el)
SU (1) SU1739856A3 (el)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8728811D0 (en) * 1987-12-09 1988-01-27 Beecham Group Plc Novel substance
US6258555B1 (en) 1987-12-09 2001-07-10 Beecham Group P.L.C. DNA encoding ACV synthetase
US5162228A (en) * 1988-12-28 1992-11-10 Takeda Chemical Industries, Ltd. Gylceraldehyde-3-phosphate dehydrogenase gene and promoter
US7049102B1 (en) 1989-09-22 2006-05-23 Board Of Trustees Of Leland Stanford University Multi-gene expression profile
ES2133140T3 (es) * 1990-01-12 1999-09-01 Glaxo Group Ltd Secuencia de adn que codifica una enzima de biosintesis de antibioticos.
JPH03254684A (ja) * 1990-03-02 1991-11-13 Asahi Chem Ind Co Ltd 新規ｃＤＮＡ化合物
CA2045951A1 (en) * 1990-07-06 1992-01-07 Steven Kovacevic Recombinant dna expression vectors and dna compounds that encode deacetylcephalosporin c synthase activity
KR100227711B1 (ko) * 1991-10-15 1999-12-01 한스 발터 라벤 7-아미노세팔로스포란산 및 7-아미노데아세틸세팔로스포란산 제조를 위한 신규한 생물학적 공정
WO2004113508A1 (en) * 2003-06-20 2004-12-29 Dsm Ip Assets B.V. Production of cephalosporin intermediates in penicillium chrysogenum
WO2006061425A2 (en) * 2004-12-10 2006-06-15 Dsm Ip Assets B.V. Production of beta-lactam antibiotics by genetically modified non-naturally producing microorganisms
ES2643476T3 (es) 2012-01-10 2017-11-23 Orchid Chemicals And Pharmaceuticals Ltd Cefalosporina hidroxilasa mutada y su aplicación en la síntesis del ácido desacetilcefalosporánico
CN103421727B (zh) * 2012-05-22 2016-03-30 上海医药工业研究院 一种转化头孢菌素c的基因工程菌及其制备方法
CN107557348B (zh) 2016-07-01 2022-07-08 百瑞全球有限公司 头孢菌素羟化酶突变体、编码该突变体的dna、使用该突变体的方法及其应用
CN111424030A (zh) * 2020-03-13 2020-07-17 常州艾米能斯生物科技有限公司 一种大规模回收线性化质粒的方法
CN112852907B (zh) * 2020-03-16 2021-11-30 中国科学院天津工业生物技术研究所 一种头孢菌素c生产菌及其制备方法与应用

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4178210A (en) 1977-03-07 1979-12-11 Massachusetts Institute Of Technology Accellular synthesis of cephalosporins
US4307192A (en) * 1979-05-17 1981-12-22 Massachusetts Institute Of Technology Cell-free synthesis of deacetoxycephalosporin C
US4762786A (en) 1984-09-27 1988-08-09 Eli Lilly And Company Vectors and conditions which allow genetic transformation of cephalosporium
EP0191221A1 (en) 1984-10-24 1986-08-20 The Regents Of The University Of California Vectors for transformation of ascomycetes
DE3683080D1 (de) * 1985-04-22 1992-02-06 Lilly Co Eli Rekombinante dns-expressionsvektoren und dns-verbindungen.
RU1780542C (ru) 1985-04-22 1992-12-07 Эли Лилли Энд Компани Способ конструировани рекомбинантной плазмидной ДНК pPS 20, кодирующей изопенициллин-N-синтетазу, способ получени штамма СернаLоSроRIUм асRемоNIUм, обладающего активностью изопенициллин-N-синтетазы
US4874703A (en) * 1985-08-26 1989-10-17 Eli Lilly And Company Expression vectors for use in E. coli
US4892819A (en) * 1985-11-25 1990-01-09 Eli Lilly And Company Recombinant DNA expression vectors and DNA compounds that encode isopenicillin N synthetase from penicillium chrysogenum
US4753881A (en) 1986-09-10 1988-06-28 Eli Lilly And Company Purified enzyme and process therefor

Also Published As

Publication number Publication date
FI880888A0 (fi) 1988-02-25
SU1739856A3 (ru) 1992-06-07
DE3855633D1 (de) 1996-12-05
IL85535A0 (en) 1988-08-31
HU205387B (en) 1992-04-28
CA1327170C (en) 1994-02-22
AU1255788A (en) 1988-09-15
US6180361B1 (en) 2001-01-30
CN1026015C (zh) 1994-09-28
KR880011337A (ko) 1988-10-27
DK102088D0 (da) 1988-02-26
ES2095204T3 (es) 1997-02-16
EP0281391B1 (en) 1996-10-30
JPS63301790A (ja) 1988-12-08
FI880888A (fi) 1988-09-05
AU608572B2 (en) 1991-04-11
HUT46359A (en) 1988-10-28
DE3855633T2 (de) 1997-03-20
BG50281A3 (en) 1992-06-15
IL85535A (en) 1994-02-27
RU1838413C (ru) 1993-08-30
EP0281391A1 (en) 1988-09-07
DK102088A (da) 1988-12-16
KR960015264B1 (ko) 1996-11-07
CN88101644A (zh) 1988-11-23

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