GB894387A - Improvements in and relating to the production of hyaluronic acid - Google Patents
Improvements in and relating to the production of hyaluronic acidInfo
- Publication number
- GB894387A GB894387A GB13971/60A GB1397160A GB894387A GB 894387 A GB894387 A GB 894387A GB 13971/60 A GB13971/60 A GB 13971/60A GB 1397160 A GB1397160 A GB 1397160A GB 894387 A GB894387 A GB 894387A
- Authority
- GB
- United Kingdom
- Prior art keywords
- hyaluronic acid
- incubation
- preferred
- acid
- broth
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
Abstract
Hyaluronic acid is produced by growing a hyoluronic-acid-producing streptococcus in an aqueous medium free from hyaluronidase-sensitive polysaccharide material and consisting essentially of casein hydrolysate and chemically defined nutrients, continuing the incubation at least to a point of maximum hyaluronic acid production, thereafter terminating the incubation and recovering the hyaluronic acid from the broth. The preferred medium is casein hydrolysate, inorganic salts yielding sodium, potassium magnesium, phosphate and sulphate ions, calcium -d-panthothenate, pyridoxine, riboflavin and glucose. In a preferred embodiment Streptococcus pyogenes is incubated at 37 DEG C. for 18 hours on the preferred medium, heated to boiling point, rapidly cooled and formalin then added. The crude broth is then treated with acetone, the lower layer is diluted with water and stirred with acetic acid and potassium acetate, and is precipitated by pouring into ethyl alcohol. It is purified by dialysis and freeze drying. Specification 676,564 is referred to.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US894387XA | 1959-05-21 | 1959-05-21 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| GB894387A true GB894387A (en) | 1962-04-18 |
Family
ID=22217649
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| GB13971/60A Expired GB894387A (en) | 1959-05-21 | 1960-04-21 | Improvements in and relating to the production of hyaluronic acid |
Country Status (1)
| Country | Link |
|---|---|
| GB (1) | GB894387A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0266578A2 (en) * | 1986-10-09 | 1988-05-11 | Mitsubishi Rayon Co., Ltd. | Method of producing hyaluronic acid |
| CN102154403A (en) * | 2010-12-22 | 2011-08-17 | 东辰控股集团有限公司 | Dissolving method and process of coarse sodium hyaluronate |
-
1960
- 1960-04-21 GB GB13971/60A patent/GB894387A/en not_active Expired
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0266578A2 (en) * | 1986-10-09 | 1988-05-11 | Mitsubishi Rayon Co., Ltd. | Method of producing hyaluronic acid |
| EP0266578A3 (en) * | 1986-10-09 | 1989-02-22 | Mitsubishi Rayon Co. Ltd. | Method of producing hyaluronic acid |
| CN102154403A (en) * | 2010-12-22 | 2011-08-17 | 东辰控股集团有限公司 | Dissolving method and process of coarse sodium hyaluronate |
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