GB736325A - Antibiotic and its production - Google Patents

Antibiotic and its production

Info

Publication number
GB736325A
GB736325A GB29978/52A GB2997852A GB736325A GB 736325 A GB736325 A GB 736325A GB 29978/52 A GB29978/52 A GB 29978/52A GB 2997852 A GB2997852 A GB 2997852A GB 736325 A GB736325 A GB 736325A
Authority
GB
United Kingdom
Prior art keywords
salt
butanol
soluble
solutions
sodium
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB29978/52A
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bristol Laboratories Inc
Original Assignee
Bristol Laboratories Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bristol Laboratories Inc filed Critical Bristol Laboratories Inc
Publication of GB736325A publication Critical patent/GB736325A/en
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
    • A01N63/28—Streptomyces
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/36—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Actinomyces; from Streptomyces (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/76—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Actinomyces; for Streptomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/465—Streptomyces

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Virology (AREA)
  • Biophysics (AREA)
  • Pest Control & Pesticides (AREA)
  • Agronomy & Crop Science (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Dentistry (AREA)
  • Environmental Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

A new antibiotic, amphomycin, is produced by cultivating Streptomyces canus, which is described, or a mutant thereof, in an aqueous, nutrient containing carbohydrate solution under submerged aerobic conditions at e.g. 24 DEG to 30 DEG C. for 2 to 7 days and pH 7.0 to 7.2. Specified carbohydrates are starch, sugars such as glucose, sucrose and lactose, glycerol, insulin and dextrin. The medium also contains (a) nitrogen source, e.g. casein and amino acids, fish meal, soybean meal, meat extracts, corn-steeping liquor, wheat steeping liquor, whey, brewers yeast, cottonseed meal, lactalbumin, distiller's solubles, liver cake, urea, nitrates, ammonium compounds and others; (b) mineral salts, e.g. sodium chloride, potassium phosphate, magnesium sulphate, sodium nitrate, and (c) buffers. The antibiotic is recovered by adjusting the filtered or unfiltered broth to approximately pH 2.0 by, e.g. hydrochloric acid and extracting with butanol, especially n-butanol, or pentanol. The sodium salt is recovered by extracting the organic solvent extract with water containing sodium hydroxide to pH 7.3 to 7.4. Further purification involves one or more of the following steps: (a) treatment of solutions especially organic solvent solutions with activated carbon; (b) formation of Reinecke's salt; (c) precipitation by means of a non-solvent, e.g. ethyl acetate; (d) precipitation at the isoelectric point namely pH 3.3 to 3.6. Amphomycin is effective against B. mycoides, B. aureus, M. tetragenus, Staph. aureus and B. subtilis; it is amphoteric forming salts with acids and metals; it is soluble in water except over the range pH 3.0 to 4.0; soluble in methanol in both acid and salt form, soluble in alcohols in acid form only; is extractable from water of pH 2.0 by butanol or pentanol but not by methyl isobutyl ketone, benzene, ether, ethyl acetate or amyl acetate; forms ammonium and Reinecke's salt; absorbs ultra-violet light only in the 210-230 m region; exhibits negative response to ninhydrin, Sakaguchi, Molisch and Ehrlich-Pauly tests; is stable for at least 10 days in aqueous solutions of pH 2 to 10; it contains carbon, hydrogen, oxygen and nitrogen.
GB29978/52A 1951-11-27 1952-11-26 Antibiotic and its production Expired GB736325A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US736325XA 1951-11-27 1951-11-27

Publications (1)

Publication Number Publication Date
GB736325A true GB736325A (en) 1955-09-07

Family

ID=22114862

Family Applications (1)

Application Number Title Priority Date Filing Date
GB29978/52A Expired GB736325A (en) 1951-11-27 1952-11-26 Antibiotic and its production

Country Status (1)

Country Link
GB (1) GB736325A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1355920A1 (en) * 2001-01-12 2003-10-29 Micrologix Biotech, Inc. Extractive purification of lipopeptide antibiotics

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7138487B2 (en) 2000-07-17 2006-11-21 Migenix Inc. Extractive purification of lipopeptide antibiotics
EP1355920A1 (en) * 2001-01-12 2003-10-29 Micrologix Biotech, Inc. Extractive purification of lipopeptide antibiotics
EP1355920A4 (en) * 2001-01-12 2005-04-20 Micrologix Biotech Inc Extractive purification of lipopeptide antibiotics

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