GB793797A - Improvements in or relating to antibiotics - Google Patents
Improvements in or relating to antibioticsInfo
- Publication number
- GB793797A GB793797A GB8795/55A GB879555A GB793797A GB 793797 A GB793797 A GB 793797A GB 8795/55 A GB8795/55 A GB 8795/55A GB 879555 A GB879555 A GB 879555A GB 793797 A GB793797 A GB 793797A
- Authority
- GB
- United Kingdom
- Prior art keywords
- per cent
- antibiotic
- solvent
- cms
- water
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
- C12P17/18—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms containing at least two hetero rings condensed among themselves or condensed with a common carbocyclic ring system, e.g. rifamycin
- C12P17/188—Heterocyclic compound containing in the condensed system at least one hetero ring having nitrogen atoms and oxygen atoms as the only ring heteroatoms
Abstract
<PICT:0793797/IV (b)/1> A new antibiotic designated E.129 is produced by culturing under aerobic conditions an antibiotic E129-producing-strain of Streptomyces E.129 nov. spec., especially Streptomyces ostreogriseus NCIB No. 8792 or NRRL No. 2558 in a nutrient medium containing an assimilable source of carbon, nitrogen and nutrient salts. It is preferred to use submerged aerobic fermentation for 16 to 28 hours at 28 DEG C. Specified sources of (a) nitrogen are ammonium sulphate and phosphate, soya bean meal and flour, maize corn meal, oat meal, corn steep liquor, distiller's solubles, casein hydrolysate and enzyme digest, peptone, tyrosine; (b) carbon are dextrin, starch and sugars such as dextrose, maltose, sucrose and glucose; (c) nutrient salts are sodium and magnesium chlorides, potassium hydrogen phosphate, calcium carbonate, calcium lactate, magnesium, zinc, ammonium and ferrous sulphates. In addition to the nutrient salts, a preferred medium contains 0.4 to 0.9, and preferably 0.7, per cent corn steep liquor (as solids) and 1.5 per cent carbohydrate especially glucose, as such, or with the further addition of 1 to 5, and especially 2.5, per cent oatmeal. Improved yields are obtained when the medium contains 30 to 135, and preferably 20 to 115 p.p.m. of a soluble salt such as ferrous sulphate, ferrous ammonium sulphate, ferric citrate and ferric chloride. Antibiotic E.129 is recovered from the filtered broth by (a) solvent extraction, or (b) adsorption. Extraction is at pH 7 to 9.5 with a water-immiscible solvent such as benzene, ethyl acetate, butyl acetate, chloroform or n-butanol. The solution so obtained is preferably washed with aqueous sodium bicarbonate, hydrochloric acid and water, dried over magnesium sulphate and the solvent removed. Alternatively, the solid obtained by removing the solvent without the above washing steps, is purified by treating with a petroleum hydrocarbon solvent such as light petroleum in order to remove impurities. Adsorption is an acidwashed charcoal or on magnesium trisilicate, followed by elution with a solvent such as n-butanol or aqueous acetone. Antibiotic E.129 is a broad spectrum antibiotic, containing 64 per cent carbon, 7 per cent hydrogen and 8 per cent nitrogen; it is readily soluble in methanol, ethanol, butanol, acetone, amyl acetate, chloroform and benzene, less soluble in ether and water and insoluble in petroleum; it is a neutral substance which does not form salts with either the common acids or common bases; it is stable over pH 2 to 10 at room temperatures, less so at higher temperatures; it melts at 121 DEG to 134 DEG C.; it exhibits no characteristic ultra-violet absorption spectrum in ethanol; it has an infra-red absorption spectrum with characteristic absorption bands at 810 cms-1, 1058 cms-1, 1022 cms-1 and 1575 cms-1, the characteristic relative intensites of these maxima being I810/I1022 greater than 0.5, I810/I1575 greater than 0.4, I1058/I1022 greater than 0.95 and I1058/I1575 greater than 0.7. R values on paper chromatogram: (1) Rf = 0.64 in 1 per cent glacial acetic acid; (2) Rf = 0.65 in 3 per cent ammonium chloride solution; (3) Rf = 0.78 in methanol/acetone/water (19 : 6 : 78). Antibiotic E.129 is distinguished from streptogramin and erythromycin. Specification 776,035 is referred to.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB8795/55A GB793797A (en) | 1955-03-25 | 1955-03-25 | Improvements in or relating to antibiotics |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB8795/55A GB793797A (en) | 1955-03-25 | 1955-03-25 | Improvements in or relating to antibiotics |
Publications (1)
Publication Number | Publication Date |
---|---|
GB793797A true GB793797A (en) | 1958-04-23 |
Family
ID=9859394
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
GB8795/55A Expired GB793797A (en) | 1955-03-25 | 1955-03-25 | Improvements in or relating to antibiotics |
Country Status (1)
Country | Link |
---|---|
GB (1) | GB793797A (en) |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US2948660A (en) * | 1957-01-10 | 1960-08-09 | Glaxo Lab Ltd | Antibiotics |
US3070505A (en) * | 1959-06-08 | 1962-12-25 | Glaxo Lab Ltd | Antibiotic process |
CN107055755A (en) * | 2016-10-11 | 2017-08-18 | 天津科技大学 | A kind of immobilized white rot fungus fluid bed dyeing waste water efficient decolorizing processing unit |
-
1955
- 1955-03-25 GB GB8795/55A patent/GB793797A/en not_active Expired
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US2948660A (en) * | 1957-01-10 | 1960-08-09 | Glaxo Lab Ltd | Antibiotics |
US3070505A (en) * | 1959-06-08 | 1962-12-25 | Glaxo Lab Ltd | Antibiotic process |
CN107055755A (en) * | 2016-10-11 | 2017-08-18 | 天津科技大学 | A kind of immobilized white rot fungus fluid bed dyeing waste water efficient decolorizing processing unit |
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