GB768364A - A new antibiotic anisomycin - Google Patents

A new antibiotic anisomycin

Info

Publication number
GB768364A
GB768364A GB16032/54A GB1603254A GB768364A GB 768364 A GB768364 A GB 768364A GB 16032/54 A GB16032/54 A GB 16032/54A GB 1603254 A GB1603254 A GB 1603254A GB 768364 A GB768364 A GB 768364A
Authority
GB
United Kingdom
Prior art keywords
per cent
anisomycin
meal
chloroform
source
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB16032/54A
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Pfizer Inc
Original Assignee
Pfizer Inc
Charles Pfizer and Co Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pfizer Inc, Charles Pfizer and Co Inc filed Critical Pfizer Inc
Priority to GB16032/54A priority Critical patent/GB768364A/en
Publication of GB768364A publication Critical patent/GB768364A/en
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
    • C12P17/18—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms containing at least two hetero rings condensed among themselves or condensed with a common carbocyclic ring system, e.g. rifamycin
    • C12P17/188—Heterocyclic compound containing in the condensed system at least one hetero ring having nitrogen atoms and oxygen atoms as the only ring heteroatoms

Abstract

A new antibiotic anisomycin (also known by the Trade name "Flagecidin") is produced by cultivating Streptomyces griseolus ATCC 11796, or Streptomyces roseochromogenus isolate No. 14576-4, or mutants thereof, in an aqueous nutrient medium under submerged aerobic conditions. A temperature of 24 DEG to 30 DEG C. and a period of 1 to 4 days is suitable. The medium contains (a) a source of carbohydrate, <PICT:0768364/IV(b)/1> <PICT:0768364/IV(b)/2> e.g. sugars, starch, glycerol and corn meal; (b) a source of nitrogen, e.g. casein, soybean meal, peanut meal, wheat glutein, cottonseed meal, lactalbumin, enzymatic digest of casein and tryptone; (c) a source of growth substances, e.g. distillers' solubles, yeast extract and molasses fermentation residues; (d) mineral salts such as sodium chloride, potassium phosphate, sodium nitrate, magnesium sulphate; and (e) trace metals such as copper, zinc and iron. A buffer, e.g. calcium carbonate and antifoaming agent may be present. Anisomycin is isolated from the liquid broth by (A) solvent extraction, or (B) adsorption. Solvent extraction is at pH6 to 10 by means of waterimmiscible solvents such as benzene, toluene, ethyl acetate, butyl acetate, methyl isobutyl ketone butanol and chloroform. Purification is obtained by treating the extract with water of below pH2.5, re-extracting the solution with the organic solvent at pH6 to 10 and precipitating by the addition of a non-solvent such as cyclohexane, hexane and carbon tetrachloride. It may be recrystallized from butanol, ethyl acetate, benzene or ethylene dichloride. Adsorption is an (a) charcoal, (b) ion-exchange resins, or (c) alumina. Anisomycin is a white basic substance effective against pathogenic protozoa and fungi; soluble in water, methyl alcohol, ethyl alcohol, dioxane and chloroform; insoluble in hexane, cyclohexane and carbontetrachloride; forms salts with acids such as hydrochloric, sulphuric and phosphoric; base has melting point of 140-141 DEG C. and [a ]D25= -30 DEG (in methanol) [a ]D25 = - 45 DEG \sB3 DEG ; exhibits three peaks in the ultra-violet at 224, 277 and 283.5mm ; dissolved in chloroform shows peaks in the infra-red at 3545, 3450, 3320, 2890, 2800, 1725, 1610, 1582, 1515, 1470, 1447, 1380, 1320, 1302, 1242, 1178, 1036 and 962; contains 63.5 per cent carbon, 7.2 per cent hydrogen, 5.2 per cent nitrogen and 24.1 per cent oxygen.
GB16032/54A 1954-05-31 1954-05-31 A new antibiotic anisomycin Expired GB768364A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
GB16032/54A GB768364A (en) 1954-05-31 1954-05-31 A new antibiotic anisomycin

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB16032/54A GB768364A (en) 1954-05-31 1954-05-31 A new antibiotic anisomycin

Publications (1)

Publication Number Publication Date
GB768364A true GB768364A (en) 1957-02-13

Family

ID=22134475

Family Applications (1)

Application Number Title Priority Date Filing Date
GB16032/54A Expired GB768364A (en) 1954-05-31 1954-05-31 A new antibiotic anisomycin

Country Status (1)

Country Link
GB (1) GB768364A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110257449A (en) * 2019-06-25 2019-09-20 大邦(湖南)生物制药有限公司 A kind of method and culture medium of fermenting and producing anisomycin

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110257449A (en) * 2019-06-25 2019-09-20 大邦(湖南)生物制药有限公司 A kind of method and culture medium of fermenting and producing anisomycin

Similar Documents

Publication Publication Date Title
GB1083662A (en) A novel antibiotic, methods for its production and therapeutic compositions containing it
GB715362A (en) Improvements in or relating to antimicrobial agent
US2928844A (en) Celesticetin, its salts and method of preparation
GB768364A (en) A new antibiotic anisomycin
GB765886A (en) A new antibiotic ristocetin and a method of producing same
US4423218A (en) Antibiotic neplanocin A
US2840578A (en) 9alpha-hydroxyprogesterone
US3142618A (en) Antifungal antibiotic humidin and process of preparation
US3112341A (en) L-delta-hydroxy-gamma-oxonorvaline
GB764198A (en) The antibiotic vengicide and processes for the preparation of the antibiotics oxytetracycline and vengicide
US2985564A (en) Production of puromycin
US3658653A (en) Process for the preparation of ergotamine and ergocryptine
ES422834A1 (en) Metabolite a-27106 and process for its preparation and use
GB779257A (en) Improvements in or relating to antibiotics and the preparation thereof
GB732150A (en) Improvements in or relating to antibiotics
GB790521A (en) Improvements in or relating to antibiotics
GB1020967A (en) Improvements in or relating to the biochemical production of ribosides or ribotides
US3923979A (en) Antibiotic 60-6 and production thereof
GB817729A (en) A new antibiotic p.a. 371
GB729208A (en) Improvements in or relating to a new antibiotic p.a. 95 and the preparation thereof
US3655877A (en) Plurallin and production thereof
US3023145A (en) Protomycin and its production
GB796493A (en) Production of tetracycline by fermentation
GB841696A (en) A new antibiotic designated r 285
GB914683A (en) The new antibiotic x-5079c and a process for its manufacture