GB770065A - Production of tetracycline by fermentation - Google Patents

Production of tetracycline by fermentation

Info

Publication number
GB770065A
GB770065A GB30108/54A GB3010854A GB770065A GB 770065 A GB770065 A GB 770065A GB 30108/54 A GB30108/54 A GB 30108/54A GB 3010854 A GB3010854 A GB 3010854A GB 770065 A GB770065 A GB 770065A
Authority
GB
United Kingdom
Prior art keywords
antibiotic
aqueous
water
acid
butanol
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB30108/54A
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bristol Laboratories Ltd
Original Assignee
Bristol Laboratories Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bristol Laboratories Ltd filed Critical Bristol Laboratories Ltd
Publication of GB770065A publication Critical patent/GB770065A/en
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C237/00—Carboxylic acid amides, the carbon skeleton of the acid part being further substituted by amino groups
    • C07C237/24—Carboxylic acid amides, the carbon skeleton of the acid part being further substituted by amino groups having the carbon atom of at least one of the carboxamide groups bound to a carbon atom of a ring other than a six-membered aromatic ring of the carbon skeleton
    • C07C237/26—Carboxylic acid amides, the carbon skeleton of the acid part being further substituted by amino groups having the carbon atom of at least one of the carboxamide groups bound to a carbon atom of a ring other than a six-membered aromatic ring of the carbon skeleton of a ring being part of a condensed ring system formed by at least four rings, e.g. tetracycline
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C2603/00—Systems containing at least three condensed rings
    • C07C2603/02—Ortho- or ortho- and peri-condensed systems
    • C07C2603/40—Ortho- or ortho- and peri-condensed systems containing four condensed rings
    • C07C2603/42—Ortho- or ortho- and peri-condensed systems containing four condensed rings containing only six-membered rings
    • C07C2603/44—Naphthacenes; Hydrogenated naphthacenes
    • C07C2603/46—1,4,4a,5,5a,6,11,12a- Octahydronaphthacenes, e.g. tetracyclines
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/465—Streptomyces

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Tetracycline (omegamycin) is produced by cultivating Streptomyces viridifaciens ATCC-11989, or a mutant thereof, in an aqueous nitrogenous nutrient medium containing carbohydrate solution under submerged aerobic conditions and recovering the antibiotic therefrom. The initial pH of the medium is 6.0-6.2, the temperature of cultivation 24 DEG to 30 DEG C., and preferably 26 DEG to 28 DEG C., and duration of cultivation 2 to 3 days. The medium contains a source of (a) carbon, e.g. starch, sucrose, glucose, maltose, dextrose, glycerol, galactose, scylose, arabinose, rhamnose, fructose, lactose, insulin and dextrins; (b) nitrogen, e.g. amino acids, casein, fish meal, soybean meal, meat extracts, liver cake, urea, nitrates, ammonium compounds, distillers' solubles, corn steep liquor, wheat steep liquor, whey, corn or wheat gluten, peptone, offals, brewers' yeast, cottonseed meal, lactalbumin and tryptone; (c) growth substances; and (d) minerals, e.g. sodium chloride, potassium phosphate, magnesium sulphate, sodium nitrate and calcium carbonate. Tetracycline is recovered from the clarified broth by (a) solvent extraction with a water-immiscible solvent such as butanol or methyl isobutyl ketone; or (b) adsorption on e.g. silica. The extract from (a) is washed, concentrated by distillation and the antibiotic precipitated by the addition of petroleum ether. The antibiotic in the form of base or acid salt depending upon pH at which extraction is made, may be further purified by treating with ammonium hydroxide and adsorption on silica. The antibiotic is eluted from the silica by means of dilute acid. Salts with acids are formed by adding an organic or inorganic acid to a suspension of the antibiotic in water. Specified are the hydrochloride, sulphate, phosphate, citrate, tartrate and gluconate. The alkali metal salts are prepared by treating an aqueous suspension of the antibiotic with an alkali metal hydroxide. Rb values are given for the following solvent systems: (a) water; (b) aqueous sodium citrate; (c) butanol saturated with water; (d) dry methyl isobutyl ketone; (e) aqueous methanolpiperidine of pH 9.5; (f) aqueous methanolglacial acetic acid; (g) water saturated butanol, piperidine and p-toluene sulphonic acid; (h) saturated butanol, piperidine, p-toluene sulphonic acid and lauric acid; and (i) isoamyl alcohol, carbon tetrachloride and aqueous sodium citrate. Reference has been directed by the Comptroller to Specification 747,779.
GB30108/54A 1953-10-19 1954-10-19 Production of tetracycline by fermentation Expired GB770065A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US770065XA 1953-10-19 1953-10-19

Publications (1)

Publication Number Publication Date
GB770065A true GB770065A (en) 1957-03-13

Family

ID=22135561

Family Applications (1)

Application Number Title Priority Date Filing Date
GB30108/54A Expired GB770065A (en) 1953-10-19 1954-10-19 Production of tetracycline by fermentation

Country Status (1)

Country Link
GB (1) GB770065A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE1094739B (en) * 1958-04-14 1960-12-15 Chinoin Gyogyszer Es Vegyeszet Process for the production of longer-acting oxytetracycline citrate

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE1094739B (en) * 1958-04-14 1960-12-15 Chinoin Gyogyszer Es Vegyeszet Process for the production of longer-acting oxytetracycline citrate

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