GB684417A - A new antibiotic and a process for the production thereof - Google Patents
A new antibiotic and a process for the production thereofInfo
- Publication number
- GB684417A GB684417A GB4960/50A GB496050A GB684417A GB 684417 A GB684417 A GB 684417A GB 4960/50 A GB4960/50 A GB 4960/50A GB 496050 A GB496050 A GB 496050A GB 684417 A GB684417 A GB 684417A
- Authority
- GB
- United Kingdom
- Prior art keywords
- antibiotic
- butanol
- water
- salts
- per cent
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P29/00—Preparation of compounds containing a naphthacene ring system, e.g. tetracycline
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/465—Streptomyces
- C12R2001/59—Streptomyces rimosus
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Medicinal Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
An antibiotic terramycin is obtained by cultivating Streptomyces rimosus (the cultural characteristics of which are described) or a mutant thereof, in an aqueous nutrient-containing solution under aerobic conditions, preferably at a temperature of 24 DEG to 30 DEG C. for 2 to 7 days under submerged conditions. The nutrient medium consists of (a) a carbohydrate source such as a sugar, starch or glycerol, (b) a source of organic nitrogen, e.g. soybean meal, wheat gluten, cottonseed meal, lactalbumen, an enzymatic digest of casein and tryptone; (b) growth substances such as distillers solubles and yeast extract; (d) mineral salts such as sodium chloride, potassium phosphate, magnesium sulphate and sodium nitrate; (e) buffering agent, e.g. calcium carbonate and, if desired, an antifoaming agent such as an animal or vegetable oil. The fermentation broth is preferably acidified to a pH below 4, the mycelium filtered off and the antibiotic recovered from the filtrate by (a) absorption on activated carbon at a pH 7, elution therefrom with acidulated water of pH 1.5 saturated with butanol and subsequent evaporation in vacuo of the eluate previously adjusted to pH 6 to 9. Alternatively, the p eluate may be extracted with butanol, the butanol extracted with N/10 hydrochloric acid and the pH of the aqueous solution adjusted to 5 by means of a synthetic anion exchange resin; or (b) extraction by means of a water-immiscible or partially water-miscible organic solvent such as butanol, amyl alcohol and the mono-phenyl ether of ethylene glycol, either at a slightly basic pH, especially 9, or at a strongly acid pH, preferably below pH 3.5. The crystalline antibiotic may be obtained by (a) partially evaporating in vacuo an acidified aqueous solution of the antibiotic at a pH 2.8; (b) adding sodium chloride and butanol to an aqueous solution of the antibiotic at pH 2.5, redissolving in methanol the precipitate which forms and adding water; and (c) using water of pH 3 and butanol in the counter-current distribution process. The antibiotic may be purified from the fermentation broth by the formation of (a) picrate; (b) aryl azo sulphonate, e.g. Orange II at pH 2 and subsequent treatment with barium chloride and (c) b -naphthalene sulphonate. Salts of organic and inorganic acids may be prepared by adding the acid, e.g. hydrochloric, sulphuric, phosphoric, citric, tartaric, and gluconic, to an aqueous suspension of the antibiotic, generally at below pH 2.5. Salts of metals may be prepared, e.g. the alkali metal salts, by adding the alkali metal hydroxide to an aqueous suspension of the antibiotic to a pH 9.5. Examples are given at the preparation of the hydrochloride and sodium salts. Terramycin is effective against gram negative and gram positive bacteria; is amphoteric, forming acid and metal salts; sparingly soluble in methanol and substantially insoluble in water and ether; the dihydrate contains 53.05 per cent C, 5.91 per cent H. 5.64 per cent N and 35.4 per cent O (by difference), the refractive indices of six-sided plate crystals is a =1.634\sB0.004, b =1.646\sB0.004, g =greater than 1.700 and a suspension in mineral oil shows absorption in the infra-red of the spectrum at the following frequencies: 3580, 3470, 3350, 3060, 1652, 1625, 1592, 1318, 1280, 1242, 1122, 1090, 1076, 1054, 1033, 1007, 938, 863, 840, 775, 708 and 679.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US1019927XA | 1949-10-13 | 1949-10-13 | |
US684417XA | 1949-10-13 | 1949-10-13 |
Publications (1)
Publication Number | Publication Date |
---|---|
GB684417A true GB684417A (en) | 1952-12-17 |
Family
ID=34829993
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
GB4960/50A Expired GB684417A (en) | 1949-10-13 | 1950-02-27 | A new antibiotic and a process for the production thereof |
Country Status (1)
Country | Link |
---|---|
GB (1) | GB684417A (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102690778A (en) * | 2012-06-06 | 2012-09-26 | 河北圣雪大成制药有限责任公司 | Method for preparing streptomyces rimosus spores |
-
1950
- 1950-02-27 GB GB4960/50A patent/GB684417A/en not_active Expired
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102690778A (en) * | 2012-06-06 | 2012-09-26 | 河北圣雪大成制药有限责任公司 | Method for preparing streptomyces rimosus spores |
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