GB821733A - Isolation process - Google Patents

Isolation process

Info

Publication number
GB821733A
GB821733A GB11154/57A GB1115457A GB821733A GB 821733 A GB821733 A GB 821733A GB 11154/57 A GB11154/57 A GB 11154/57A GB 1115457 A GB1115457 A GB 1115457A GB 821733 A GB821733 A GB 821733A
Authority
GB
United Kingdom
Prior art keywords
acetate
ethyl
fermentation medium
methyl
butanol
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB11154/57A
Inventor
Christopher Towers Calam
Philip John Curtis
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Imperial Chemical Industries Ltd
Original Assignee
Imperial Chemical Industries Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Imperial Chemical Industries Ltd filed Critical Imperial Chemical Industries Ltd
Priority to GB11154/57A priority Critical patent/GB821733A/en
Priority to FR1203766D priority patent/FR1203766A/en
Publication of GB821733A publication Critical patent/GB821733A/en
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
    • C12P17/18—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms containing at least two hetero rings condensed among themselves or condensed with a common carbocyclic ring system, e.g. rifamycin
    • C12P17/182—Heterocyclic compounds containing nitrogen atoms as the only ring heteroatoms in the condensed system
    • C12P17/183—Heterocyclic compounds containing nitrogen atoms as the only ring heteroatoms in the condensed system containing an indolo[4,3-F,G]quinoleine nucleus, e.g. compound containing the lysergic acid nucleus as well as the dimeric ergot nucleus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P27/00—Preparation of compounds containing a gibbane ring system, e.g. gibberellin

Landscapes

  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Gibberellic acid is isolated from an aqueous fermentation medium thereof in a process which comprises extracting the said aqueous fermentation medium with a water-immiscible or partially water-miscible organic solvent selected from the group consisting of esters, ketones, alcohols and ethers. Specified solvents are ethyl acetate, n-butyl acetate, isoamyl acetate, diethyl carbonate, ethyl propionate, ethyl n-butyrate, n-butyl lactate, isopropyl acetate, b -ethoxyethyl acetate, methoxybutyl acetate, methyl n-propyl ketone, cyclohexanone, diethylketone, methyl isobutyl ketone, methyl cyclohexanone, methyl ethyl ketone, benzyl alcohol, sec.-butanol, cyclohexanol, n-butanol, diethyl ether and 1:1-dimethoxyethane. Mixtures of solvents, e.g. n-butanol and n-butylacetate or isoamyl acetate and n-amyl acetate may also be employed. Prior to extraction the aqueous fermentation medium is preferably adjusted to a pH of 1.5-4.0. A suitable starting material is an aqueous filtrate obtained from a fermentation medium made by growing the fungus Gibberella fujikuroi. The organic solvent extract so obtained may be extracted with a buffer solution of pH 6-8 and the buffer extract acidified to a pH of 3-4, the gibberellic acid is then extracted with ethyl acetate. Examples describe the extraction of gibberillic acid from aqueous fermentation media employing the solvents listed above.
GB11154/57A 1957-04-05 1957-04-05 Isolation process Expired GB821733A (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
GB11154/57A GB821733A (en) 1957-04-05 1957-04-05 Isolation process
FR1203766D FR1203766A (en) 1957-04-05 1958-04-04 Method of isolating Gibberellic acid from aqueous fermentation media

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
GB11154/57A GB821733A (en) 1957-04-05 1957-04-05 Isolation process
GB2950288X 1957-04-05
GB1203766X 1957-04-05
GB1088911X 1957-04-05

Publications (1)

Publication Number Publication Date
GB821733A true GB821733A (en) 1959-10-14

Family

ID=34916416

Family Applications (1)

Application Number Title Priority Date Filing Date
GB11154/57A Expired GB821733A (en) 1957-04-05 1957-04-05 Isolation process

Country Status (2)

Country Link
FR (1) FR1203766A (en)
GB (1) GB821733A (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2005501864A (en) * 2001-08-31 2005-01-20 オーストラリアン バイオメディカル カンパニー ピーティーワイ エルティーディー Gibberellin preparation and use in diabetes
CN104672190A (en) * 2014-12-12 2015-06-03 江西新瑞丰生化有限公司 Method for recrystallizing gibberellin mother liquor

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE1268088B (en) * 1961-06-20 1968-05-16 Melle Bezons Process for extracting citric, itaconic or lactic acid produced by fermentation of technical sugary substances

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2005501864A (en) * 2001-08-31 2005-01-20 オーストラリアン バイオメディカル カンパニー ピーティーワイ エルティーディー Gibberellin preparation and use in diabetes
CN104672190A (en) * 2014-12-12 2015-06-03 江西新瑞丰生化有限公司 Method for recrystallizing gibberellin mother liquor
CN104672190B (en) * 2014-12-12 2017-04-19 江西新瑞丰生化有限公司 Method for recrystallizing gibberellin mother liquor

Also Published As

Publication number Publication date
FR1203766A (en) 1960-01-21

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