GB307844A - Process for the manufacture of sexual hormones - Google Patents

Abstract

307,844. Schering-Kahlbaum Akt.- Ges. March 14, 1928, [Convention date]. Hormones.-Sexual hormones are obtained from faeces by extraction of the material with a water-insoluble organic solvent, or in the case of dried material, with a water soluble organic solvent, saponification of the residue from evaporation of the solvent, and extraction of the hormone from the unsaponifiable portion; or by extraction with a water soluble organic solvent, preferably in the presence of an alkaline earth, and separation of the hormone from the extract in the usual manner. In both processes an inert adsorbent such as kaolin, animal charcoal, or silica gel may be added, and the starting material may previously have been treated with water containing alkali to obtain a solution from which the hormones are extracted In obtaining female hormones material is employed from a woman or animal in a state of pregnancy or at or about the time of menstruation or heat. According to examples (1) dried faces are extracted with ether, the residue saponified with alcoholic potash, and the unsaponiflable residue extracted with ether: (2) faeces are extracted with methyl alcohol, the filtered extract evaporated and extracted with ether, the residue extracted with 40 per cent methyl alcohol with the addition of calcium hydroxide, the extract filtered, the alcohol evaporated, and the remaining aqueous solution extracted with ether; (3) faeces are worked up as in example 2 and the product obtained ground to a powder with barium hydroxide, extracted with boiling water, the barium precipitated by carbon-dioxide, and the aqueous solution filtered; (4) faeces are diluted with 0.4 per cent caustic soda lye, extracted with ether, and the etheral residue worked up as in example 2; (5) faeces are mixed with silica gel and calcium hydroxide, extracted with aqueous alcohol, the extract concentrated to expel the alcohol, and the remaining aqueous solution extracted with ether. The Specification as open to inspection under Sect. 91 (3) (a) refers to the use also of gall as a source of hormone, and to the preliminary treatment of the starting material with water containing acid, and contains additional examples according to one of which gall is used in place of faeces in example 4 (above), and according to the second of which ox-gall is heated with methyl alcohol and calcium hydroxide, the liquid filtered, the alcohol evaporated, the aqueous residue extracted with ether, the residue ground with calcium hydroxide, treated with 40 per cent methyl alcohol, the alcohol distilled off, the aqueous residue extracted with ether and the ether evaporated. This subject-matter does not appear in the Specification as accepted.