GB201007055D0 - Method and apparatus for the imaging of a labelled sample - Google Patents

Method and apparatus for the imaging of a labelled sample

Info

Publication number
GB201007055D0
GB201007055D0 GBGB1007055.5A GB201007055A GB201007055D0 GB 201007055 D0 GB201007055 D0 GB 201007055D0 GB 201007055 A GB201007055 A GB 201007055A GB 201007055 D0 GB201007055 D0 GB 201007055D0
Authority
GB
United Kingdom
Prior art keywords
imaging
labelled sample
labelled
sample
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
GBGB1007055.5A
Other languages
English (en)
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Katholieke Universiteit Leuven
Vlaams Instituut voor Biotechnologie VIB
Original Assignee
Katholieke Universiteit Leuven
Vlaams Instituut voor Biotechnologie VIB
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Katholieke Universiteit Leuven, Vlaams Instituut voor Biotechnologie VIB filed Critical Katholieke Universiteit Leuven
Priority to GBGB1007055.5A priority Critical patent/GB201007055D0/en
Publication of GB201007055D0 publication Critical patent/GB201007055D0/en
Priority to EP11717268A priority patent/EP2564257A1/en
Priority to PCT/EP2011/056784 priority patent/WO2011135049A1/en
Priority to JP2013506667A priority patent/JP5969463B2/ja
Priority to US13/661,364 priority patent/US9569828B2/en
Ceased legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T5/00—Image enhancement or restoration
    • G06T5/50—Image enhancement or restoration using two or more images, e.g. averaging or subtraction
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6408—Fluorescence; Phosphorescence with measurement of decay time, time resolved fluorescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G01N21/6458—Fluorescence microscopy
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/0052—Optical details of the image generation
    • G02B21/0076—Optical details of the image generation arrangements using fluorescence or luminescence
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/008—Details of detection or image processing, including general computer control
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
    • G02B21/365—Control or image processing arrangements for digital or video microscopes
    • G02B21/367—Control or image processing arrangements for digital or video microscopes providing an output produced by processing a plurality of individual source images, e.g. image tiling, montage, composite images, depth sectioning, image comparison
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B27/00—Optical systems or apparatus not provided for by any of the groups G02B1/00 - G02B26/00, G02B30/00
    • G02B27/58—Optics for apodization or superresolution; Optical synthetic aperture systems
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06V—IMAGE OR VIDEO RECOGNITION OR UNDERSTANDING
    • G06V20/00—Scenes; Scene-specific elements
    • G06V20/60—Type of objects
    • G06V20/69—Microscopic objects, e.g. biological cells or cellular parts
    • G06V20/693—Acquisition
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
    • G01N2021/6441—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks with two or more labels
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/10—Image acquisition modality
    • G06T2207/10064—Fluorescence image
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/20—Special algorithmic details
    • G06T2207/20212—Image combination
    • G06T2207/20224—Image subtraction
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/30—Subject of image; Context of image processing
    • G06T2207/30004—Biomedical image processing
    • G06T2207/30024—Cell structures in vitro; Tissue sections in vitro
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/30—Subject of image; Context of image processing
    • G06T2207/30004—Biomedical image processing
    • G06T2207/30096—Tumor; Lesion

Landscapes

  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Optics & Photonics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Biochemistry (AREA)
  • Multimedia (AREA)
  • Theoretical Computer Science (AREA)
  • Computer Vision & Pattern Recognition (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Microscoopes, Condenser (AREA)
GBGB1007055.5A 2010-04-28 2010-04-28 Method and apparatus for the imaging of a labelled sample Ceased GB201007055D0 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
GBGB1007055.5A GB201007055D0 (en) 2010-04-28 2010-04-28 Method and apparatus for the imaging of a labelled sample
EP11717268A EP2564257A1 (en) 2010-04-28 2011-04-28 Method and apparatus for the imaging of a labelled biological sample
PCT/EP2011/056784 WO2011135049A1 (en) 2010-04-28 2011-04-28 Method and apparatus for the imaging of a labelled biological sample
JP2013506667A JP5969463B2 (ja) 2010-04-28 2011-04-28 ラベル付けされた生体試料の画像化のための方法と装置
US13/661,364 US9569828B2 (en) 2010-04-28 2012-10-26 Method and apparatus for the imaging of a labeled biological sample

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GBGB1007055.5A GB201007055D0 (en) 2010-04-28 2010-04-28 Method and apparatus for the imaging of a labelled sample

Publications (1)

Publication Number Publication Date
GB201007055D0 true GB201007055D0 (en) 2010-06-09

Family

ID=42270933

Family Applications (1)

Application Number Title Priority Date Filing Date
GBGB1007055.5A Ceased GB201007055D0 (en) 2010-04-28 2010-04-28 Method and apparatus for the imaging of a labelled sample

Country Status (5)

Country Link
US (1) US9569828B2 (enExample)
EP (1) EP2564257A1 (enExample)
JP (1) JP5969463B2 (enExample)
GB (1) GB201007055D0 (enExample)
WO (1) WO2011135049A1 (enExample)

Families Citing this family (36)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102011077269A1 (de) * 2011-06-09 2012-12-13 Carl Zeiss Microimaging Gmbh Hochauflösende Lumineszenzmikroskopie
JP5714997B2 (ja) * 2011-07-14 2015-05-07 オリンパス株式会社 蛍光顕微鏡装置
JP6389763B2 (ja) * 2012-03-02 2018-09-12 ザ リージェンツ オブ ザ ユニヴァーシティー オブ カリフォルニアＴｈｅ Ｒｅｇｅｎｔｓ Ｏｆ Ｔｈｅ Ｕｎｉｖｅｒｓｉｔｙ Ｏｆ Ｃａｌｉｆｏｒｎｉａ 時間分解蛍光画像化およびパルス成形のためのシステムおよび方法
DE102012205032A1 (de) 2012-03-28 2013-10-02 Carl Zeiss Microscopy Gmbh Hochauflösende Fluoreszenzmikroskopie
JP6335160B2 (ja) * 2012-04-13 2018-05-30 バイオアキシアル エスエーエス 光学測定方法および光学測定装置
WO2014121003A1 (en) * 2013-02-04 2014-08-07 The General Hospital Corporation System and method for fluorescence detection
US9570487B2 (en) * 2013-02-11 2017-02-14 The United States of America, Naval Undersea Warfare Center Optical output photodetector
DE102014102425B4 (de) * 2014-02-25 2018-06-28 Carl Zeiss Meditec Ag Mikroskopsystem und Mikroskopieverfahren unter Verwendung digitaler Marker
JP6355961B2 (ja) * 2014-04-30 2018-07-11 オリンパス株式会社 標本観察装置
WO2016145633A1 (zh) * 2015-03-18 2016-09-22 国立清华大学 高速三维成像之光学系统
US10802262B2 (en) * 2015-10-29 2020-10-13 The Board Of Trustees Of The Leland Stanford Junior University Methods and systems for imaging a biological sample
WO2017090210A1 (ja) * 2015-11-27 2017-06-01 株式会社ニコン 顕微鏡、観察方法、及び画像処理プログラム
DE102015016353A1 (de) 2015-12-16 2017-06-22 Horst Wochnowski PALM-Verfahren mit mehreren sich nur marginal voneinander unterscheidenden Fluorophoren
WO2017110974A1 (ja) * 2015-12-24 2017-06-29 コニカミノルタ株式会社 画像処理装置及びプログラム
CA3032089A1 (en) * 2016-08-03 2018-02-08 Royal Melbourne Institute Of Technology A method for distinguishing between more than one fluorescent species present in a sample
CN107144951B (zh) * 2017-06-30 2023-03-21 中国计量大学 一种基于半球微结构的超分辨显微装置
WO2019135069A1 (en) * 2018-01-02 2019-07-11 King's College London Method and system for localisation microscopy
WO2019145767A1 (en) * 2018-01-25 2019-08-01 King Abdullah University Of Science And Technology Deep-learning based structure reconstruction method and apparatus
DE102018210606B4 (de) * 2018-06-28 2025-10-02 Carl Zeiss Microscopy Gmbh Mikroskopisches Abbildungsverfahren unter Verwendung eines Korrekturfaktors
US10724956B1 (en) * 2019-02-01 2020-07-28 Essen Instruments, Inc. Spectral unmixing
CN111913293B (zh) * 2019-12-12 2022-04-05 南开大学 一种荧光激发光谱超分辨显微系统和方法
EP4177333A4 (en) * 2020-07-02 2023-12-27 FUJIFILM Corporation INFORMATION PROCESSING DEVICE, INFORMATION PROCESSING METHOD, AND PROGRAM
EP3961194B1 (en) * 2020-08-25 2023-11-08 Korea Advanced Institute of Science and Technology Method and apparatus for multiplexed imaging of biomolecules through iterative unmixing of fluorophore signals
KR102655200B1 (ko) * 2020-08-25 2024-04-08 한국과학기술원 반복색분리를 통한 분자 다중 이미징 방법 및 장치
DE102020131047B4 (de) * 2020-11-24 2024-08-22 Abberior Instruments Gmbh Verfahren und Vorrichtung zur Aufnahme nanoskopischer Bilder mehrfach gefärbter Proben
EP4092466A1 (en) * 2021-05-21 2022-11-23 Leica Microsystems CMS GmbH Method for obtaining an operating value of an illumination parameter for examining a fluorescent sample, method for examining a fluorescent sample, microscope system and computer program
TWI832469B (zh) * 2021-10-06 2024-02-11 中央研究院 檢測系統
DE102022131450A1 (de) * 2022-11-28 2024-05-29 Carl Zeiss Microscopy Gmbh Verfahren und Vorrichtung zum Aufbereiten von Daten zum Identifizieren von Analyten
DE102022131451A1 (de) * 2022-11-28 2024-05-29 Carl Zeiss Microscopy Gmbh Verfahren und Vorrichtung zum Bestimmen einer Signal-Zusammensetzung von Signalfolgen einer Bildfolge
DE102022131449A1 (de) * 2022-11-28 2024-05-29 Carl Zeiss Microscopy Gmbh Verfahren und Vorrichtung zum Aufbereiten von Daten zum Identifizieren von Analyten
DE102022131444A1 (de) * 2022-11-28 2024-05-29 Carl Zeiss Microscopy Gmbh Verfahren zum Identifizieren von Analyten in einer Bildfolge
DE102022131447A1 (de) * 2022-11-28 2024-05-29 Carl Zeiss Microscopy Gmbh Verfahren und Vorrichtung zum Aufbereiten von Daten zum Identifizieren von Analyten
DE102022131445A1 (de) * 2022-11-28 2024-05-29 Carl Zeiss Microscopy Gmbh Verfahren und Vorrichtung zum Aufbereiten von Daten zum Identifizieren von Analyten
DE102022131446A1 (de) * 2022-11-28 2024-05-29 Carl Zeiss Microscopy Gmbh Verfahren und Vorrichtung zum Aufbereiten von Daten zum Identifizieren von Analyten
DE102022131448A1 (de) * 2022-11-28 2024-05-29 Carl Zeiss Microscopy Gmbh Verfahren und Vorrichtung zum Aufbereiten von Daten zum Identifizieren von Analyten
DE102023122213A1 (de) * 2023-08-18 2025-02-20 Carl Zeiss Microscopy Gmbh Verfahren zum Bereitstellen von Quelldaten zum Trainieren oder Validieren eines Verarbeitungsmodells zum Verarbeiten von Analyt-Bildfolgen

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3242372A (en) 1962-05-31 1966-03-22 Sylvania Electric Prod Incandescent lamp containing iodine, oxygen and inert gas
US4542459A (en) 1982-11-26 1985-09-17 General Electric Company Matched filter for x-ray hybrid subtraction
US6061476A (en) 1997-11-24 2000-05-09 Cognex Corporation Method and apparatus using image subtraction and dynamic thresholding
DE19908883A1 (de) * 1999-03-02 2000-09-07 Rainer Heintzmann Verfahren zur Erhöhung der Auflösung optischer Abbildung
JP2000325295A (ja) * 1999-05-21 2000-11-28 Fuji Photo Film Co Ltd 蛍光診断情報出力方法および装置
JP2001299676A (ja) * 2000-04-25 2001-10-30 Fuji Photo Film Co Ltd センチネルリンパ節検出方法および検出システム
JP4545337B2 (ja) * 2001-03-23 2010-09-15 オリンパス株式会社 顕微鏡
JP2004184379A (ja) * 2002-12-06 2004-07-02 Mitsubishi Rayon Co Ltd マイクロアレイの読取方法
US7262419B2 (en) * 2004-05-05 2007-08-28 1192062 Alberta Ltd. Fluorescence absorption microscopy
JP4339746B2 (ja) * 2004-05-17 2009-10-07 オリンパス株式会社 蛍光検出装置の励起光照射タイミングの決定方法
JP5028008B2 (ja) * 2004-12-08 2012-09-19 オリンパス株式会社 蛍光内視鏡装置
EP3203235A1 (en) 2005-05-23 2017-08-09 Harald F. Hess Optical microscopy with phototransformable optical labels
US20070057211A1 (en) * 2005-05-25 2007-03-15 Karsten Bahlman Multifocal imaging systems and method
ATE441103T1 (de) 2005-07-22 2009-09-15 Zeiss Carl Microimaging Gmbh Auflísungsgesteigerte lumineszenz-mikroskopie
JP2007222073A (ja) * 2006-02-23 2007-09-06 Yamaguchi Univ 画像処理により細胞運動特性を評価する方法、そのための画像処理装置及び画像処理プログラム
US7772569B2 (en) * 2008-04-01 2010-08-10 The Jackson Laboratory 3D biplane microscopy
GB0814297D0 (en) * 2008-08-05 2008-09-10 Ge Healthcare Uk Ltd Microscopy

Also Published As

Publication number Publication date
US20140118524A1 (en) 2014-05-01
US9569828B2 (en) 2017-02-14
JP5969463B2 (ja) 2016-08-17
JP2013531221A (ja) 2013-08-01
EP2564257A1 (en) 2013-03-06
WO2011135049A1 (en) 2011-11-03

Similar Documents

Publication Publication Date Title
GB201007055D0 (en) Method and apparatus for the imaging of a labelled sample
GB2493891B (en) Method and apparatus for determining location using signals-of-opportunity
PL2466322T3 (pl) Sposób i urządzenie diagnozowania transformatora
EP2546793A4 (en) METHOD AND APPARATUS FOR PROVIDING A LIST OF APPLICATIONS
GB201003363D0 (en) Measurement method and apparatus
GB201003599D0 (en) Measurement method and apparatus
EP2611359A4 (en) Apparatus and method for imaging feet
GB2483077A8 (en) Sample testing apparatus and method
EP2596320A4 (en) METHOD AND APPARATUS FOR IMAGING
GB201013896D0 (en) Apparatus and method for measuring distance
PL2630466T3 (pl) Urządzenie do pobierania próbki i sposób pobierania próbki
EP2657644A4 (en) POSITIONING DEVICE AND POSITIONING METHOD
EP2567247A4 (en) PROCESS AND DEVICE FOR QUICK TTFF
GB2486937B (en) Apparatus and method for sensing and identifying specimen slides
GB201005989D0 (en) A method and apparatus for determining missalignment
PL2619552T3 (pl) Urządzenie i sposób kontroli substancji
GB201117081D0 (en) Apparatus and method for radiation analysis
GB201019488D0 (en) Blood analysis apparatus and method
GB0905298D0 (en) Apparatus and method for ferromagnetic object detector
EP2590024A4 (en) METHOD AND APPARATUS FOR IMAGING
GB201008736D0 (en) Inspection apparatus and method
EP2623957A4 (en) IMAGE ANALYSIS METHOD AND IMAGE ANALYSIS DEVICE
EP2778659A4 (en) IMAGE ANALYSIS METHOD AND IMAGE ANALYSIS DEVICE
GB201014476D0 (en) Method and apparatus for the detection of plagiarised material
EP2623956A4 (en) IMAGE ANALYSIS METHOD AND IMAGE ANALYSIS DEVICE

Legal Events

Date Code Title Description
AT Applications terminated before publication under section 16(1)