GB1603772A - Antibody immobilized in a acrylamide copolymer matrix - Google Patents
Antibody immobilized in a acrylamide copolymer matrix Download PDFInfo
- Publication number
- GB1603772A GB1603772A GB16822/78A GB1682278A GB1603772A GB 1603772 A GB1603772 A GB 1603772A GB 16822/78 A GB16822/78 A GB 16822/78A GB 1682278 A GB1682278 A GB 1682278A GB 1603772 A GB1603772 A GB 1603772A
- Authority
- GB
- United Kingdom
- Prior art keywords
- acrylamide
- antibody
- immobilised
- copolymer
- polymer matrix
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 239000011159 matrix material Substances 0.000 title claims description 40
- 229920006322 acrylamide copolymer Polymers 0.000 title claims description 11
- HRPVXLWXLXDGHG-UHFFFAOYSA-N Acrylamide Chemical compound NC(=O)C=C HRPVXLWXLXDGHG-UHFFFAOYSA-N 0.000 claims description 28
- 229920000642 polymer Polymers 0.000 claims description 28
- CERQOIWHTDAKMF-UHFFFAOYSA-N Methacrylic acid Chemical group CC(=C)C(O)=O CERQOIWHTDAKMF-UHFFFAOYSA-N 0.000 claims description 17
- 229920001577 copolymer Polymers 0.000 claims description 16
- 239000000178 monomer Substances 0.000 claims description 13
- SMZOUWXMTYCWNB-UHFFFAOYSA-N 2-(2-methoxy-5-methylphenyl)ethanamine Chemical compound COC1=CC=C(C)C=C1CCN SMZOUWXMTYCWNB-UHFFFAOYSA-N 0.000 claims description 12
- NIXOWILDQLNWCW-UHFFFAOYSA-N 2-Propenoic acid Natural products OC(=O)C=C NIXOWILDQLNWCW-UHFFFAOYSA-N 0.000 claims description 12
- 238000000034 method Methods 0.000 claims description 12
- 239000002253 acid Substances 0.000 claims description 8
- 150000003839 salts Chemical class 0.000 claims description 8
- FQPSGWSUVKBHSU-UHFFFAOYSA-N methacrylamide Chemical compound CC(=C)C(N)=O FQPSGWSUVKBHSU-UHFFFAOYSA-N 0.000 claims description 6
- -1 and N Inorganic materials 0.000 claims description 5
- 229910052757 nitrogen Inorganic materials 0.000 claims description 5
- 229910052783 alkali metal Inorganic materials 0.000 claims description 3
- 229910052784 alkaline earth metal Inorganic materials 0.000 claims description 3
- 150000002148 esters Chemical class 0.000 claims description 3
- 125000004433 nitrogen atom Chemical group N* 0.000 claims description 3
- 238000004519 manufacturing process Methods 0.000 claims description 2
- 239000003795 chemical substances by application Substances 0.000 claims 2
- 239000000243 solution Substances 0.000 description 16
- 229940088597 hormone Drugs 0.000 description 8
- 239000005556 hormone Substances 0.000 description 8
- 239000000126 substance Substances 0.000 description 8
- 229940034208 thyroxine Drugs 0.000 description 8
- XUIIKFGFIJCVMT-GFCCVEGCSA-N D-thyroxine Chemical compound IC1=CC(C[C@@H](N)C(O)=O)=CC(I)=C1OC1=CC(I)=C(O)C(I)=C1 XUIIKFGFIJCVMT-GFCCVEGCSA-N 0.000 description 7
- 229920002401 polyacrylamide Polymers 0.000 description 7
- XUIIKFGFIJCVMT-UHFFFAOYSA-N thyroxine-binding globulin Natural products IC1=CC(CC([NH3+])C([O-])=O)=CC(I)=C1OC1=CC(I)=C(O)C(I)=C1 XUIIKFGFIJCVMT-UHFFFAOYSA-N 0.000 description 7
- 239000003814 drug Substances 0.000 description 6
- 230000000694 effects Effects 0.000 description 6
- JYGXADMDTFJGBT-VWUMJDOOSA-N hydrocortisone Chemical compound O=C1CC[C@]2(C)[C@H]3[C@@H](O)C[C@](C)([C@@](CC4)(O)C(=O)CO)[C@@H]4[C@@H]3CCC2=C1 JYGXADMDTFJGBT-VWUMJDOOSA-N 0.000 description 6
- 239000000203 mixture Substances 0.000 description 6
- 210000002966 serum Anatomy 0.000 description 6
- 229940088594 vitamin Drugs 0.000 description 6
- 239000011782 vitamin Substances 0.000 description 6
- 229930003231 vitamin Natural products 0.000 description 6
- 235000013343 vitamin Nutrition 0.000 description 6
- 230000002860 competitive effect Effects 0.000 description 5
- 230000009260 cross reactivity Effects 0.000 description 5
- 230000015572 biosynthetic process Effects 0.000 description 4
- 238000006243 chemical reaction Methods 0.000 description 4
- 229960001348 estriol Drugs 0.000 description 4
- 229960000890 hydrocortisone Drugs 0.000 description 4
- 239000008363 phosphate buffer Substances 0.000 description 4
- 239000000700 radioactive tracer Substances 0.000 description 4
- 238000011156 evaluation Methods 0.000 description 3
- 239000011521 glass Substances 0.000 description 3
- 238000002649 immunization Methods 0.000 description 3
- 238000011534 incubation Methods 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 159000000000 sodium salts Chemical class 0.000 description 3
- 150000003431 steroids Chemical class 0.000 description 3
- 238000003786 synthesis reaction Methods 0.000 description 3
- PROQIPRRNZUXQM-UHFFFAOYSA-N (16alpha,17betaOH)-Estra-1,3,5(10)-triene-3,16,17-triol Natural products OC1=CC=C2C3CCC(C)(C(C(O)C4)O)C4C3CCC2=C1 PROQIPRRNZUXQM-UHFFFAOYSA-N 0.000 description 2
- 102000009027 Albumins Human genes 0.000 description 2
- 108010088751 Albumins Proteins 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 2
- 241000283973 Oryctolagus cuniculus Species 0.000 description 2
- 102000057297 Pepsin A Human genes 0.000 description 2
- 108090000284 Pepsin A Proteins 0.000 description 2
- RJKFOVLPORLFTN-LEKSSAKUSA-N Progesterone Chemical compound C1CC2=CC(=O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H](C(=O)C)[C@@]1(C)CC2 RJKFOVLPORLFTN-LEKSSAKUSA-N 0.000 description 2
- AUNGANRZJHBGPY-SCRDCRAPSA-N Riboflavin Chemical compound OC[C@@H](O)[C@@H](O)[C@@H](O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-SCRDCRAPSA-N 0.000 description 2
- MUMGGOZAMZWBJJ-DYKIIFRCSA-N Testostosterone Chemical compound O=C1CC[C@]2(C)[C@H]3CC[C@](C)([C@H](CC4)O)[C@@H]4[C@@H]3CCC2=C1 MUMGGOZAMZWBJJ-DYKIIFRCSA-N 0.000 description 2
- AUYYCJSJGJYCDS-LBPRGKRZSA-N Thyrolar Chemical compound IC1=CC(C[C@H](N)C(O)=O)=CC(I)=C1OC1=CC=C(O)C(I)=C1 AUYYCJSJGJYCDS-LBPRGKRZSA-N 0.000 description 2
- ZORQMBLUMWNJEQ-ZXXIGWHRSA-N [(8r,9s,13s,14s,16r,17r)-16,17-dihydroxy-13-methyl-6,7,8,9,11,12,14,15,16,17-decahydrocyclopenta[a]phenanthren-3-yl] hydrogen sulfate Chemical compound OS(=O)(=O)OC1=CC=C2[C@H]3CC[C@](C)([C@H]([C@H](O)C4)O)[C@@H]4[C@@H]3CCC2=C1 ZORQMBLUMWNJEQ-ZXXIGWHRSA-N 0.000 description 2
- 150000001340 alkali metals Chemical class 0.000 description 2
- 238000003556 assay Methods 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 230000008878 coupling Effects 0.000 description 2
- 238000010168 coupling process Methods 0.000 description 2
- 238000005859 coupling reaction Methods 0.000 description 2
- 229940088598 enzyme Drugs 0.000 description 2
- PROQIPRRNZUXQM-ZXXIGWHRSA-N estriol Chemical compound OC1=CC=C2[C@H]3CC[C@](C)([C@H]([C@H](O)C4)O)[C@@H]4[C@@H]3CCC2=C1 PROQIPRRNZUXQM-ZXXIGWHRSA-N 0.000 description 2
- UZKIAJMSMKLBQE-JRSYHJKYSA-N estriol 3-O-(beta-D-glucuronide) Chemical compound C([C@@H]1[C@@H](C2=CC=3)CC[C@]4([C@H]1C[C@@H](O)[C@@H]4O)C)CC2=CC=3O[C@@H]1O[C@H](C(O)=O)[C@@H](O)[C@H](O)[C@H]1O UZKIAJMSMKLBQE-JRSYHJKYSA-N 0.000 description 2
- 230000002209 hydrophobic effect Effects 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 229940111202 pepsin Drugs 0.000 description 2
- 239000011148 porous material Substances 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 238000011084 recovery Methods 0.000 description 2
- 229910052708 sodium Inorganic materials 0.000 description 2
- 239000011734 sodium Substances 0.000 description 2
- 238000000638 solvent extraction Methods 0.000 description 2
- 229940036555 thyroid hormone Drugs 0.000 description 2
- 239000005495 thyroid hormone Substances 0.000 description 2
- 229940035722 triiodothyronine Drugs 0.000 description 2
- VOXZDWNPVJITMN-ZBRFXRBCSA-N 17β-estradiol Chemical compound OC1=CC=C2[C@H]3CC[C@](C)([C@H](CC4)O)[C@@H]4[C@@H]3CCC2=C1 VOXZDWNPVJITMN-ZBRFXRBCSA-N 0.000 description 1
- YPEMKASELPCGPB-UHFFFAOYSA-N 2-methylprop-2-enoic acid;prop-2-enamide Chemical compound NC(=O)C=C.CC(=C)C(O)=O YPEMKASELPCGPB-UHFFFAOYSA-N 0.000 description 1
- 229920001817 Agar Polymers 0.000 description 1
- AUNGANRZJHBGPY-UHFFFAOYSA-N D-Lyxoflavin Natural products OCC(O)C(O)C(O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-UHFFFAOYSA-N 0.000 description 1
- WDJUZGPOPHTGOT-OAXVISGBSA-N Digitoxin Natural products O([C@H]1[C@@H](C)O[C@@H](O[C@@H]2C[C@@H]3[C@@](C)([C@@H]4[C@H]([C@]5(O)[C@@](C)([C@H](C6=CC(=O)OC6)CC5)CC4)CC3)CC2)C[C@H]1O)[C@H]1O[C@@H](C)[C@H](O[C@H]2O[C@@H](C)[C@@H](O)[C@@H](O)C2)[C@@H](O)C1 WDJUZGPOPHTGOT-OAXVISGBSA-N 0.000 description 1
- LTMHDMANZUZIPE-AMTYYWEZSA-N Digoxin Natural products O([C@H]1[C@H](C)O[C@H](O[C@@H]2C[C@@H]3[C@@](C)([C@@H]4[C@H]([C@]5(O)[C@](C)([C@H](O)C4)[C@H](C4=CC(=O)OC4)CC5)CC3)CC2)C[C@@H]1O)[C@H]1O[C@H](C)[C@@H](O[C@H]2O[C@@H](C)[C@H](O)[C@@H](O)C2)[C@@H](O)C1 LTMHDMANZUZIPE-AMTYYWEZSA-N 0.000 description 1
- 101150064205 ESR1 gene Proteins 0.000 description 1
- 229920013685 Estron Polymers 0.000 description 1
- DNXHEGUUPJUMQT-CBZIJGRNSA-N Estrone Chemical compound OC1=CC=C2[C@H]3CC[C@](C)(C(CC4)=O)[C@@H]4[C@@H]3CCC2=C1 DNXHEGUUPJUMQT-CBZIJGRNSA-N 0.000 description 1
- KWYHDKDOAIKMQN-UHFFFAOYSA-N N,N,N',N'-tetramethylethylenediamine Chemical compound CN(C)CCN(C)C KWYHDKDOAIKMQN-UHFFFAOYSA-N 0.000 description 1
- 239000004952 Polyamide Substances 0.000 description 1
- QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 description 1
- VEUACKUBDLVUAC-UHFFFAOYSA-N [Na].[Ca] Chemical compound [Na].[Ca] VEUACKUBDLVUAC-UHFFFAOYSA-N 0.000 description 1
- 150000007513 acids Chemical class 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 150000001342 alkaline earth metals Chemical class 0.000 description 1
- 239000012670 alkaline solution Substances 0.000 description 1
- 229940035676 analgesics Drugs 0.000 description 1
- 239000000730 antalgic agent Substances 0.000 description 1
- 239000003146 anticoagulant agent Substances 0.000 description 1
- 229940127219 anticoagulant drug Drugs 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 230000003139 buffering effect Effects 0.000 description 1
- 159000000007 calcium salts Chemical class 0.000 description 1
- 150000001718 carbodiimides Chemical class 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 239000007979 citrate buffer Substances 0.000 description 1
- 230000037029 cross reaction Effects 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- DOBMPNYZJYQDGZ-UHFFFAOYSA-N dicoumarol Chemical compound C1=CC=CC2=C1OC(=O)C(CC=1C(OC3=CC=CC=C3C=1O)=O)=C2O DOBMPNYZJYQDGZ-UHFFFAOYSA-N 0.000 description 1
- 229960001912 dicoumarol Drugs 0.000 description 1
- HIZKPJUTKKJDGA-UHFFFAOYSA-N dicumarol Natural products O=C1OC2=CC=CC=C2C(=O)C1CC1C(=O)C2=CC=CC=C2OC1=O HIZKPJUTKKJDGA-UHFFFAOYSA-N 0.000 description 1
- 229960000648 digitoxin Drugs 0.000 description 1
- WDJUZGPOPHTGOT-XUDUSOBPSA-N digitoxin Chemical compound C1[C@H](O)[C@H](O)[C@@H](C)O[C@H]1O[C@@H]1[C@@H](C)O[C@@H](O[C@@H]2[C@H](O[C@@H](O[C@@H]3C[C@@H]4[C@]([C@@H]5[C@H]([C@]6(CC[C@@H]([C@@]6(C)CC5)C=5COC(=O)C=5)O)CC4)(C)CC3)C[C@@H]2O)C)C[C@@H]1O WDJUZGPOPHTGOT-XUDUSOBPSA-N 0.000 description 1
- 229960005156 digoxin Drugs 0.000 description 1
- LTMHDMANZUZIPE-PUGKRICDSA-N digoxin Chemical compound C1[C@H](O)[C@H](O)[C@@H](C)O[C@H]1O[C@@H]1[C@@H](C)O[C@@H](O[C@@H]2[C@H](O[C@@H](O[C@@H]3C[C@@H]4[C@]([C@@H]5[C@H]([C@]6(CC[C@@H]([C@@]6(C)[C@H](O)C5)C=5COC(=O)C=5)O)CC4)(C)CC3)C[C@@H]2O)C)C[C@@H]1O LTMHDMANZUZIPE-PUGKRICDSA-N 0.000 description 1
- LTMHDMANZUZIPE-UHFFFAOYSA-N digoxine Natural products C1C(O)C(O)C(C)OC1OC1C(C)OC(OC2C(OC(OC3CC4C(C5C(C6(CCC(C6(C)C(O)C5)C=5COC(=O)C=5)O)CC4)(C)CC3)CC2O)C)CC1O LTMHDMANZUZIPE-UHFFFAOYSA-N 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 230000009881 electrostatic interaction Effects 0.000 description 1
- 230000008030 elimination Effects 0.000 description 1
- 238000003379 elimination reaction Methods 0.000 description 1
- 238000010828 elution Methods 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 230000007071 enzymatic hydrolysis Effects 0.000 description 1
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 description 1
- 238000006911 enzymatic reaction Methods 0.000 description 1
- 229960005309 estradiol Drugs 0.000 description 1
- 229930182833 estradiol Natural products 0.000 description 1
- 229940011871 estrogen Drugs 0.000 description 1
- 239000000262 estrogen Substances 0.000 description 1
- 229960003399 estrone Drugs 0.000 description 1
- LSSGNEFHGVQOPA-AWEZNQCLSA-N ethyl (2s)-2-amino-3-[4-(4-hydroxy-3,5-diiodophenoxy)-3,5-diiodophenyl]propanoate Chemical compound IC1=CC(C[C@H](N)C(=O)OCC)=CC(I)=C1OC1=CC(I)=C(O)C(I)=C1 LSSGNEFHGVQOPA-AWEZNQCLSA-N 0.000 description 1
- 125000004494 ethyl ester group Chemical group 0.000 description 1
- 230000007717 exclusion Effects 0.000 description 1
- 230000001747 exhibiting effect Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 125000000524 functional group Chemical group 0.000 description 1
- 239000000499 gel Substances 0.000 description 1
- 150000008134 glucuronides Chemical group 0.000 description 1
- 229930182470 glycoside Natural products 0.000 description 1
- 150000002338 glycosides Chemical class 0.000 description 1
- 229920001519 homopolymer Polymers 0.000 description 1
- 230000001900 immune effect Effects 0.000 description 1
- 230000016784 immunoglobulin production Effects 0.000 description 1
- 230000002452 interceptive effect Effects 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 229920003145 methacrylic acid copolymer Polymers 0.000 description 1
- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 description 1
- ZIUHHBKFKCYYJD-UHFFFAOYSA-N n,n'-methylenebisacrylamide Chemical compound C=CC(=O)NCNC(=O)C=C ZIUHHBKFKCYYJD-UHFFFAOYSA-N 0.000 description 1
- 238000006386 neutralization reaction Methods 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N phenol group Chemical group C1(=CC=CC=C1)O ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 1
- 229920002647 polyamide Polymers 0.000 description 1
- 230000000379 polymerizing effect Effects 0.000 description 1
- 159000000001 potassium salts Chemical class 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 239000000186 progesterone Substances 0.000 description 1
- 229960003387 progesterone Drugs 0.000 description 1
- 238000010526 radical polymerization reaction Methods 0.000 description 1
- 229960002477 riboflavin Drugs 0.000 description 1
- 235000019192 riboflavin Nutrition 0.000 description 1
- 239000002151 riboflavin Substances 0.000 description 1
- YGSDEFSMJLZEOE-UHFFFAOYSA-M salicylate Chemical compound OC1=CC=CC=C1C([O-])=O YGSDEFSMJLZEOE-UHFFFAOYSA-M 0.000 description 1
- 229960001860 salicylate Drugs 0.000 description 1
- 150000003873 salicylate salts Chemical class 0.000 description 1
- 239000007790 solid phase Substances 0.000 description 1
- 239000003270 steroid hormone Substances 0.000 description 1
- 229960003604 testosterone Drugs 0.000 description 1
- 229940082632 vitamin b12 and folic acid Drugs 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 230000003313 weakening effect Effects 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/544—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being organic
- G01N33/545—Synthetic resin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/395—Antibodies; Immunoglobulins; Immune serum, e.g. antilymphocytic serum
- A61K39/44—Antibodies bound to carriers
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/807—Apparatus included in process claim, e.g. physical support structures
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/815—Test for named compound or class of compounds
- Y10S436/817—Steroids or hormones
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- Hematology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Urology & Nephrology (AREA)
- Biochemistry (AREA)
- Epidemiology (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Cell Biology (AREA)
- Biotechnology (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Mycology (AREA)
- Pharmacology & Pharmacy (AREA)
- Food Science & Technology (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Peptides Or Proteins (AREA)
- Addition Polymer Or Copolymer, Post-Treatments, Or Chemical Modifications (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Medicinal Preparation (AREA)
- Compositions Of Macromolecular Compounds (AREA)
- Paints Or Removers (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE2721267A DE2721267C2 (de) | 1977-05-11 | 1977-05-11 | Antikörpergel |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| GB1603772A true GB1603772A (en) | 1981-11-25 |
Family
ID=6008655
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| GB16822/78A Expired GB1603772A (en) | 1977-05-11 | 1978-04-27 | Antibody immobilized in a acrylamide copolymer matrix |
Country Status (24)
| Country | Link |
|---|---|
| US (1) | US4235867A (enExample) |
| JP (1) | JPS5417116A (enExample) |
| AT (1) | AT364856B (enExample) |
| AU (1) | AU519148B2 (enExample) |
| BE (1) | BE866491A (enExample) |
| BR (1) | BR7802600A (enExample) |
| CH (1) | CH637218A5 (enExample) |
| DD (1) | DD136185A5 (enExample) |
| DE (1) | DE2721267C2 (enExample) |
| DK (1) | DK181478A (enExample) |
| ES (1) | ES469830A1 (enExample) |
| FI (1) | FI781220A7 (enExample) |
| FR (1) | FR2390731A1 (enExample) |
| GB (1) | GB1603772A (enExample) |
| GR (1) | GR64474B (enExample) |
| HU (1) | HU178485B (enExample) |
| IL (1) | IL54558A (enExample) |
| LU (1) | LU79535A1 (enExample) |
| NL (1) | NL7804466A (enExample) |
| NO (1) | NO781473L (enExample) |
| NZ (1) | NZ187071A (enExample) |
| PL (1) | PL121950B1 (enExample) |
| SE (1) | SE7804801L (enExample) |
| ZA (1) | ZA782358B (enExample) |
Families Citing this family (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2458070A1 (fr) * | 1979-05-28 | 1980-12-26 | Compiegne Inst Technol Surf Ac | Dispositif pour l'identification et le dosage de substances biochimiques antigeniques dans les fluides biologiques |
| NL8120020A (nl) * | 1980-02-05 | 1982-01-04 | Japan Atomic Energy Res Inst | Eenheidssysteem voor medische analyse en werkwijze ter vervaardiging ervan. |
| JPS5729949A (en) * | 1980-07-30 | 1982-02-18 | Nippon Koutai Kenkyusho:Kk | Determination of sugar branch related to cancer and diagnostic technique of cancer |
| US4540660A (en) * | 1983-04-07 | 1985-09-10 | Daryl Laboratories, Inc. | Substrate for fluoroimmunoassay of biological fluids |
| JPS61228351A (ja) * | 1985-04-02 | 1986-10-11 | Kyoto Ikagaku Kenkyusho:Kk | 糞便中のヘモグロビンの検出方法 |
| CA1339670C (en) * | 1986-04-30 | 1998-02-10 | Ronald C. Kennedy | Polyamide resin and resin-protide, and methods for preparation of reagents and of immunodiagnostic testing |
| DE3921498A1 (de) * | 1988-09-28 | 1990-03-29 | Bayer Ag | Polymer-gebundene-farbstoffe, verfahren zu deren herstellung und verwendung |
| US5210289A (en) * | 1990-06-18 | 1993-05-11 | Eastman Kodak Company | Carboxy containing monomers |
| US5262297A (en) * | 1990-06-18 | 1993-11-16 | Eastman Kodak Company | Specific binding analytical and separation methods using carboxy containing polymers |
| US5149737A (en) * | 1990-06-18 | 1992-09-22 | Eastman Kodak Company | Carboxy containing monomers and polymers and latices prepared from same |
| US5147777A (en) * | 1990-06-18 | 1992-09-15 | Eastman Kodak Company | Biologically active reagents prepared from carboxy-containing polymer, analytical element and methods of use |
| US5424219A (en) * | 1991-10-25 | 1995-06-13 | Cytech Biomedical, Inc. | Method of performing assays for biomolecules and solid supports for use in such methods |
| EP2192409B1 (en) * | 2008-11-26 | 2014-04-23 | Corning Incorporated | Label independent detection biosensor composition and methods thereof |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3925017A (en) * | 1973-05-01 | 1975-12-09 | Wisconsin Alumni Res Found | Preparation of dry, porous gel particles having high water regain for liquid sampling |
| US4061466A (en) * | 1974-10-16 | 1977-12-06 | Ingvar Gosta Holger Sjoholm | Biologically active composition and the use thereof |
-
1977
- 1977-05-11 DE DE2721267A patent/DE2721267C2/de not_active Expired
-
1978
- 1978-04-19 GR GR56025A patent/GR64474B/el unknown
- 1978-04-20 IL IL54558A patent/IL54558A/xx unknown
- 1978-04-20 FI FI781220A patent/FI781220A7/fi not_active Application Discontinuation
- 1978-04-21 AU AU35364/78A patent/AU519148B2/en not_active Expired
- 1978-04-24 US US05/899,744 patent/US4235867A/en not_active Expired - Lifetime
- 1978-04-24 AT AT0290978A patent/AT364856B/de not_active IP Right Cessation
- 1978-04-25 ZA ZA00782358A patent/ZA782358B/xx unknown
- 1978-04-26 SE SE7804801A patent/SE7804801L/xx unknown
- 1978-04-26 NL NL7804466A patent/NL7804466A/xx not_active Application Discontinuation
- 1978-04-26 DK DK181478A patent/DK181478A/da not_active Application Discontinuation
- 1978-04-26 FR FR7812344A patent/FR2390731A1/fr active Granted
- 1978-04-26 LU LU79535A patent/LU79535A1/de unknown
- 1978-04-26 NZ NZ187071A patent/NZ187071A/xx unknown
- 1978-04-26 BR BR7802600A patent/BR7802600A/pt unknown
- 1978-04-26 CH CH454178A patent/CH637218A5/de not_active IP Right Cessation
- 1978-04-26 NO NO78781473A patent/NO781473L/no unknown
- 1978-04-27 HU HU78CA424A patent/HU178485B/hu unknown
- 1978-04-27 JP JP5072678A patent/JPS5417116A/ja active Granted
- 1978-04-27 BE BE187209A patent/BE866491A/xx not_active IP Right Cessation
- 1978-04-27 PL PL1978206415A patent/PL121950B1/pl unknown
- 1978-04-27 GB GB16822/78A patent/GB1603772A/en not_active Expired
- 1978-04-27 ES ES469830A patent/ES469830A1/es not_active Expired
- 1978-04-27 DD DD78205046A patent/DD136185A5/xx unknown
Also Published As
| Publication number | Publication date |
|---|---|
| DD136185A5 (de) | 1979-06-20 |
| BE866491A (fr) | 1978-08-14 |
| BR7802600A (pt) | 1979-01-23 |
| IL54558A (en) | 1982-01-31 |
| NL7804466A (nl) | 1978-11-14 |
| DE2721267A1 (de) | 1978-11-23 |
| AU519148B2 (en) | 1981-11-12 |
| FR2390731A1 (fr) | 1978-12-08 |
| PL121950B1 (en) | 1982-06-30 |
| NO781473L (no) | 1978-11-14 |
| GR64474B (en) | 1980-03-27 |
| US4235867A (en) | 1980-11-25 |
| DE2721267C2 (de) | 1985-05-02 |
| FI781220A7 (fi) | 1978-11-12 |
| ZA782358B (en) | 1979-04-25 |
| IL54558A0 (en) | 1978-07-31 |
| FR2390731B1 (enExample) | 1984-05-04 |
| JPS6229747B2 (enExample) | 1987-06-27 |
| SE7804801L (sv) | 1978-11-12 |
| ATA290978A (de) | 1981-04-15 |
| HU178485B (en) | 1982-05-28 |
| LU79535A1 (de) | 1978-09-29 |
| AU3536478A (en) | 1979-10-25 |
| ES469830A1 (es) | 1978-12-16 |
| AT364856B (de) | 1981-11-25 |
| NZ187071A (en) | 1980-12-19 |
| DK181478A (da) | 1978-11-12 |
| JPS5417116A (en) | 1979-02-08 |
| CH637218A5 (de) | 1983-07-15 |
| PL206415A1 (enExample) | 1980-11-17 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| DE69113872T2 (de) | Biologisch aktive Reagenzien aus Carboxylgruppenhaltigen Polymerisaten, analytisches Element und Verfahren zur Verwendung. | |
| US4048298A (en) | Solid phase double-antibody radioimmunoassay procedure | |
| US5168057A (en) | Trifunctional conjugates | |
| US4235867A (en) | Acrylamide copolymer matrix for radioimmune assay techniques | |
| JPS5896616A (ja) | 抗原、抗体またはハプテンの検出試薬およびそれに使用するラテックス並びにそれらの製法 | |
| JPH0737988B2 (ja) | イオン捕捉イムノアッセイ法および装置 | |
| GB1586506A (en) | Immunochemical process and reagents for the determination of haptens | |
| US4450239A (en) | Method of determining pregnanediol in female urine and test device for use therein | |
| DK165381B (da) | Immunoanalysemetode til bestemmelse af ligander ved hjaelp af biotin og antibiotin | |
| JPS6315551B2 (enExample) | ||
| US4256724A (en) | Method for non-covalent coating of antibodies on solid substrates | |
| US4235865A (en) | Method for the determination of unbound hormones and pharmaceuticals | |
| CA1288691C (en) | Stable immobilized hapten reagent for use in heterogeneous immunometric assays | |
| GB2260609A (en) | A separation method employing precursor species | |
| US4235866A (en) | Process for the total determination of hormones and pharmaceuticals | |
| EP0687910B1 (en) | Test kit and method for competitive specific binding assay | |
| JP2947600B2 (ja) | 免疫測定法 | |
| CA1143653A (en) | Hapten bound to carboxyl containing water-soluble mono-olefinic polmeric compound | |
| EP0685740A2 (en) | Separation-free specific binding assays using anti-inhibitor antibodies | |
| CN120059276B (zh) | 一种荧光复合微球及其制备方法和应用 | |
| EP0537826B1 (en) | Diagnostic test kit and specific binding assay using a peroxidase label and a modulator of signal | |
| US5916757A (en) | Specific binding assay using enzyme inhibitor and anti-inhibitor antibodies | |
| JP2893127B2 (ja) | イムノアッセイにおける偽陰性反応の抑制方法 | |
| NL8120020A (nl) | Eenheidssysteem voor medische analyse en werkwijze ter vervaardiging ervan. |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PS | Patent sealed [section 19, patents act 1949] | ||
| PCNP | Patent ceased through non-payment of renewal fee |