GB1576856A - Method and apparatus for determination of cell fragility - Google Patents
Method and apparatus for determination of cell fragility Download PDFInfo
- Publication number
- GB1576856A GB1576856A GB25055/77A GB2505577A GB1576856A GB 1576856 A GB1576856 A GB 1576856A GB 25055/77 A GB25055/77 A GB 25055/77A GB 2505577 A GB2505577 A GB 2505577A GB 1576856 A GB1576856 A GB 1576856A
- Authority
- GB
- United Kingdom
- Prior art keywords
- solution
- capillary tube
- cells
- sample
- tube
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 238000000034 method Methods 0.000 title claims description 50
- 239000000243 solution Substances 0.000 claims description 41
- 230000003204 osmotic effect Effects 0.000 claims description 32
- 239000012266 salt solution Substances 0.000 claims description 24
- 206010018910 Haemolysis Diseases 0.000 claims description 18
- 230000008588 hemolysis Effects 0.000 claims description 18
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 15
- 230000003287 optical effect Effects 0.000 claims description 12
- 230000008859 change Effects 0.000 claims description 9
- 210000004369 blood Anatomy 0.000 claims description 7
- 239000008280 blood Substances 0.000 claims description 7
- 230000007423 decrease Effects 0.000 claims description 6
- 238000012544 monitoring process Methods 0.000 claims description 6
- 239000011780 sodium chloride Substances 0.000 claims description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 5
- 230000009977 dual effect Effects 0.000 claims description 4
- 150000003839 salts Chemical class 0.000 claims description 4
- 238000002156 mixing Methods 0.000 claims description 3
- 239000000463 material Substances 0.000 claims description 2
- 230000000717 retained effect Effects 0.000 claims description 2
- 239000007864 aqueous solution Substances 0.000 claims 3
- 239000003795 chemical substances by application Substances 0.000 claims 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-M Chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 claims 1
- 230000005540 biological transmission Effects 0.000 claims 1
- 210000004027 cell Anatomy 0.000 description 37
- 210000003743 erythrocyte Anatomy 0.000 description 31
- 239000000523 sample Substances 0.000 description 21
- 102000001554 Hemoglobins Human genes 0.000 description 10
- 108010054147 Hemoglobins Proteins 0.000 description 10
- 239000012530 fluid Substances 0.000 description 10
- 238000012360 testing method Methods 0.000 description 10
- 239000012528 membrane Substances 0.000 description 7
- 230000005484 gravity Effects 0.000 description 5
- 238000000502 dialysis Methods 0.000 description 4
- 230000002159 abnormal effect Effects 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 3
- 210000000601 blood cell Anatomy 0.000 description 3
- 239000012153 distilled water Substances 0.000 description 3
- 238000011534 incubation Methods 0.000 description 3
- 238000011835 investigation Methods 0.000 description 3
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 2
- HTTJABKRGRZYRN-UHFFFAOYSA-N Heparin Chemical compound OC1C(NC(=O)C)C(O)OC(COS(O)(=O)=O)C1OC1C(OS(O)(=O)=O)C(O)C(OC2C(C(OS(O)(=O)=O)C(OC3C(C(O)C(O)C(O3)C(O)=O)OS(O)(=O)=O)C(CO)O2)NS(O)(=O)=O)C(C(O)=O)O1 HTTJABKRGRZYRN-UHFFFAOYSA-N 0.000 description 2
- 230000004075 alteration Effects 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 230000003247 decreasing effect Effects 0.000 description 2
- 238000009826 distribution Methods 0.000 description 2
- 229960002897 heparin Drugs 0.000 description 2
- 229920000669 heparin Polymers 0.000 description 2
- 230000014759 maintenance of location Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 230000004044 response Effects 0.000 description 2
- 239000012488 sample solution Substances 0.000 description 2
- 208000007056 sickle cell anemia Diseases 0.000 description 2
- 239000011550 stock solution Substances 0.000 description 2
- 101100283604 Caenorhabditis elegans pigk-1 gene Proteins 0.000 description 1
- 229920000298 Cellophane Polymers 0.000 description 1
- 239000004809 Teflon Substances 0.000 description 1
- 229920006362 Teflon® Polymers 0.000 description 1
- 230000005856 abnormality Effects 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- 239000003146 anticoagulant agent Substances 0.000 description 1
- 229940127219 anticoagulant drug Drugs 0.000 description 1
- 238000010420 art technique Methods 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000000432 density-gradient centrifugation Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 239000003599 detergent Substances 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 208000010643 digestive system disease Diseases 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000003480 eluent Substances 0.000 description 1
- 238000010828 elution Methods 0.000 description 1
- 230000002949 hemolytic effect Effects 0.000 description 1
- 208000024557 hepatobiliary disease Diseases 0.000 description 1
- 244000144980 herd Species 0.000 description 1
- 210000000265 leukocyte Anatomy 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 230000007480 spreading Effects 0.000 description 1
- 238000003892 spreading Methods 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 238000002834 transmittance Methods 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 238000004804 winding Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N13/00—Investigating surface or boundary effects, e.g. wetting power; Investigating diffusion effects; Analysing materials by determining surface, boundary, or diffusion effects
- G01N13/04—Investigating osmotic effects
Landscapes
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US05/713,045 US4040742A (en) | 1976-08-09 | 1976-08-09 | Capillary flow method and apparatus for determination of cell osmotic fragility |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| GB1576856A true GB1576856A (en) | 1980-10-15 |
Family
ID=24864521
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| GB25055/77A Expired GB1576856A (en) | 1976-08-09 | 1977-06-15 | Method and apparatus for determination of cell fragility |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US4040742A (OSRAM) |
| JP (1) | JPS6049865B2 (OSRAM) |
| CA (1) | CA1068587A (OSRAM) |
| DE (1) | DE2733409A1 (OSRAM) |
| FR (1) | FR2361657A1 (OSRAM) |
| GB (1) | GB1576856A (OSRAM) |
| IL (1) | IL52311A (OSRAM) |
| SE (1) | SE7707048L (OSRAM) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2475227A1 (fr) * | 1980-02-05 | 1981-08-07 | Coulter Electronics | Procede et dispositif pour examiner les changements de parametres de cellules biologiques |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4151089A (en) * | 1978-05-17 | 1979-04-24 | The United States Of America As Represented By The Secretary Of The Department Of Health, Education And Welfare | Device for high efficiency continuous countercurrent extraction using a rotating helical tube |
| US4255788A (en) * | 1979-03-01 | 1981-03-10 | American National Red Cross | Phasing detector for continuous flow systems |
| EP0626067A4 (en) * | 1992-02-07 | 1997-06-25 | Abbott Lab | METHOD FOR ACCURATELY COUNTING AND SENSITIVELY CLASSIFYING HETEROGENEOUS CELL POPULATIONS UNDER CYTOLYTIC PROCESSING CONDITIONS. |
| EP2199791B1 (de) * | 2008-12-19 | 2019-05-01 | F. Hoffmann-La Roche AG | Verfahren zum Bestimmen der Hämolyse einer Blutprobe sowie Vorrichtung |
| US8026102B2 (en) * | 2009-01-21 | 2011-09-27 | Blaze Medical Devices, LLC | Apparatus and method to characterize blood and red blood cells via erythrocyte membrane fragility quantification |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US2673789A (en) * | 1945-11-10 | 1954-03-30 | American Optical Corp | Applicator for hemolyzing whole blood |
| US3300385A (en) * | 1961-08-17 | 1967-01-24 | Yeda Res & Dev | Method for measuring osmotic fragility of red blood corpuscles |
| GB1053947A (OSRAM) * | 1962-09-05 | |||
| US3606539A (en) * | 1968-11-13 | 1971-09-20 | American Optical Corp | Apparatus and method for measuring osmotic fragility of red blood cells |
-
1976
- 1976-08-09 US US05/713,045 patent/US4040742A/en not_active Expired - Lifetime
-
1977
- 1977-06-13 CA CA280,442A patent/CA1068587A/en not_active Expired
- 1977-06-14 IL IL52311A patent/IL52311A/xx unknown
- 1977-06-15 GB GB25055/77A patent/GB1576856A/en not_active Expired
- 1977-06-17 SE SE7707048A patent/SE7707048L/ not_active Application Discontinuation
- 1977-06-22 FR FR7719066A patent/FR2361657A1/fr active Granted
- 1977-07-23 DE DE19772733409 patent/DE2733409A1/de not_active Withdrawn
- 1977-08-08 JP JP52094922A patent/JPS6049865B2/ja not_active Expired
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2475227A1 (fr) * | 1980-02-05 | 1981-08-07 | Coulter Electronics | Procede et dispositif pour examiner les changements de parametres de cellules biologiques |
Also Published As
| Publication number | Publication date |
|---|---|
| FR2361657A1 (fr) | 1978-03-10 |
| IL52311A (en) | 1979-03-12 |
| DE2733409A1 (de) | 1978-02-16 |
| FR2361657B1 (OSRAM) | 1982-06-18 |
| SE7707048L (sv) | 1978-02-10 |
| JPS5351796A (en) | 1978-05-11 |
| IL52311A0 (en) | 1977-08-31 |
| US4040742A (en) | 1977-08-09 |
| JPS6049865B2 (ja) | 1985-11-05 |
| CA1068587A (en) | 1979-12-25 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PS | Patent sealed [section 19, patents act 1949] | ||
| PCNP | Patent ceased through non-payment of renewal fee |