JP6335802B2 - 液体試料イメージング装置及び方法 - Google Patents
液体試料イメージング装置及び方法 Download PDFInfo
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Description
血液がカートリッジの第1チャンバに挿入され、
血液が毛細管作用によって流体構造で第1チャンバから第2検出チャンバ内に流れ、
血液が前記流体構造に流れる一方、溶血剤の少なくとも一断片および染色剤の少なくとも一断片からなる異なる試薬の緩やかな再懸濁があり、
前記第2検出チャンバ内の溶解し染色された血液の画像を取り込み、
前記第2検出チャンバ内で溶解し染色された血液の得られた画像を所定の画像のプロパティのしきい値と比較することによって、画像細胞分割および白血球の分類を実行することを特徴とする方法。
カートリッジが停止している間に、前記血液計量チャンバは、毛管作用によって血液が充填され、
カートリッジが回転し、計量された血液が前記血液計量チャンバから前記下流チャンバに遠心分離によって動かされ、
前記下流チャンバ内の濃厚赤血球と血漿との間の界面の光学イメージング測定によってヘマトクリット割合が決定される、請求項1に記載の方法。
カートリッジが停止している間に、前記血液計量チャンバは、毛管作用によって血液が充填され、
カートリッジが回転し、計量された血液が前記血液計量チャンバから前記下流チャンバに遠心分離によって動かされ、
血漿の予め定義された容積が、さらなる分析の目的のために、前記下流チャンバからカートリッジの他の下流部分に取り出される、先行する節に記載の方法。
装置内の血液試料挿入用の入口と、
あらかじめ定義された寸法を有する少なくとも一つの検出チャンバと、
あらかじめ定義された寸法を有する少なくとも一つの接続チャンバと、
前記検出チャンバを照射するための光学手段と、
前記検出チャンバの画像取得のための光学手段とを含み、
少なくとも一つの接続チャンバは前記入口を検出チャンバへ連結し、前記接続チャンバは少なくとも二種類の乾燥試薬を含み、乾燥試薬は(i)血液試料中の赤血球の選択的溶解のための溶血剤と、(ii)血液試料中の白血球を選択的に染色するための染色剤を含み、
前記乾燥試薬は、前記接続チャンバのあらかじめ定義された拡張部に格納されており、
前記の接続チャンバは、血液試料が前記接続チャンバを通って前記検出チャンバに流れる間に前記乾燥試薬が緩やかに再懸濁することによって、少なくとも一つの反応領域を含み、
セグメント化された細胞の光学的および形態学的特性によって前記取得された画像からの細胞のセグメンテーションと分類が行われ、
分類された細胞から差分および/または絶対的な白血球計数が行われる、ことを特徴とする装置。
前記計量チャンバに具現された少なくとも一つのスプリット機能と、
少なくとも一つの下流チャンバと、
少なくとも一つのオーバーフローチャンバと、
少なくとも一つの廃棄チャンバと、
少なくとも一つの流れ遮断弁と、を有する追加の流体本体を含む、先行する節のいずれかに記載の装置であって、
前記流体本体が当該流体本体を含む平面に垂直な回転軸に対する極座標で表された節17に記載の装置。
Claims (20)
- 液体試料を撮像する際に使用するための装置であって、
試料を当該装置に受け入れるための入口と、
接続導管と、
前記試料を光学的に検出するための検出チャンバとを含み、
前記接続導管は、前記入口を前記検出チャンバに接続し、
前記接続導管は、前記試料が前記接続導管を通過するとき、前記試料と反応するための1以上の乾燥試薬を含み、
前記接続導管の少なくとも一つの寸法は、前記入口の最小寸法よりも小さく、前記検出チャンバの少なくとも一つの寸法は、前記接続導管の最小寸法よりも小さく、
前記試料は血液試料であり、前記1以上の乾燥試薬は、前記血液試料中の赤血球の選択的溶解のための溶血剤と、前記血液試料中の白血球を選択的に染色するための染色剤とを含む、ことを特徴とする装置。 - 前記接続導管は、主要導管部と、前記主要導管部から延びる1以上の突出部とを含み、前記乾燥試薬は前記1以上の突出部および前記1以上の突出部間の各接合領域に格納され、前記主要導管部は、前記乾燥試薬の漸進的な再懸濁が発生する可能性のある反応領域を提供する、請求項1に記載の装置。
- 前記突出部は、主要部と前記接合領域におけるネック部とを含み、前記ネック部は、前記主要部よりも前記主要導管部に沿ってより小さな断面積を有する、請求項2に記載の装置。
- 前記接続導管は、蛇行形状である、請求項1から3のいずれかに記載の装置。
- 前記接続導管は、さらに乾燥形態にある安定剤を含む、請求項1から4のいずれかに記載の装置。
- 前記接続導管は、さらに乾燥形態にある界面活性剤を含む、請求項1から5のいずれかに記載の装置。
- 試料の所定の容積を保持するように構成された計量チャンバであって、前記入口と前記接続導管とに流体接続された計量チャンバと、
前記計量チャンバと前記接続導管との間で試料を分割するために、前記計量チャンバおよび前記接続導管の間に配置されたスプリット機能と、
前記計量チャンバと流体接続され、前記試料の所定の容積を受け取るように構成された下流チャンバとをさらに含み、
当該装置は、前記下流チャンバに液体の流れを駆動するために回転軸を中心に回転するように配置されている、請求項1から6のいずれかに記載の装置。 - 前記下流チャンバと流体接続するオーバーフローチャンバをさらに含み、前記下流チャンバと流体接続するサイフォンをさらに含み、当該装置が遠心力を受け、サイフォンがプライミングされたとき、試料の所定の容積が前記下流チャンバ内の試料から吸い上げられるように、サイフォンの入口は前記下流チャンバの一部から径方向内側に配置される、請求項7に記載の装置。
- 乾燥抗凝固剤が前記計量チャンバに格納される、請求項7または請求項8に記載の装置。
- 前記計量チャンバは表面張力バリアを含み、表面張力バリアが前記計量チャンバと前記下流チャンバの間の毛管駆動流を停止するように構成される、請求項7から9のいずれかに記載の装置。
- 廃棄チャンバをさらに含み、前記廃棄チャンバは前記検出チャンバと流体接続され、前記検出チャンバから遠心力で駆動されたオーバーフローを受け取るために、前記検出チャンバから径方向内側に配置される、請求項7から10のいずれかに記載の装置。
- 請求項1から11のいずれかに記載の装置を含む、試料を撮像するためのシステムであって、当該システムは、
前記検出チャンバまたは前記下流チャンバ、あるいはその両方の中の試料の少なくとも一つの画像を取得する撮像部をさらに含むことを特徴とするシステム。 - 当該装置を回転させるためのドライブをさらに含む、請求項12に記載のシステム。
- 前記検出チャンバ内の試料の少なくとも一つの画像を処理するように構成されたプロセッサをさらに含み、試料が血液試料である場合、画像における染色された細胞を分割するために、前記プロセッサは、下流チャンバ内の血液試料のヘマトクリット割合を決定するようにさらに構成された、請求項12または13に記載のシステム。
- 液体試料を撮像する方法であって、前記方法は、
カートリッジの第1チャンバに試料を挿入するステップと、
毛細管現象によって第1チャンバから接続導管を通って検出チャンバへ試料を流れさせるステップと、
試料が前記接続導管を通って流れる間に徐々に試料中の1以上の乾燥試薬を再懸濁させるステップと、
前記検出チャンバ内の試料の少なくとも一つの画像を取得するステップとを含み、
前記試料は血液試料であり、前記1以上の乾燥試薬は、前記血液試料中の赤血球の選択的溶解のための溶血剤と、前記血液試料中の白血球を選択的に染色するための染色剤とを含む、
ことを特徴とする方法。 - 画像細胞分割および白血球の分類を実行するステップをさらに含む、請求項15に記載の方法。
- 毛管作用によって計量チャンバを試料で充填するステップをさらに含む、請求項15または16に記載の方法。
- 前記第1チャンバと前記計量チャンバは共通の入口から充填される、請求項17に記載の方法。
- 前記計量チャンバ内に含まれる試料が遠心力によって前記計量チャンバから前記下流チャンバに移動されるように、前記カートリッジを回転させるステップをさらに含む、請求項17または請求項18に記載の方法。
- 前記下流チャンバ内の血漿から細胞物質を分離するために前記カートリッジを回転させるステップと、いったん細胞物質が分離されると前記下流チャンバ内の試料を撮像するステップとをさらに含む、請求項19に記載の方法。
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