GB1501512A - Process for the separation of micro-organisms from a culture medium - Google Patents

Process for the separation of micro-organisms from a culture medium

Info

Publication number
GB1501512A
GB1501512A GB1571075A GB1571075A GB1501512A GB 1501512 A GB1501512 A GB 1501512A GB 1571075 A GB1571075 A GB 1571075A GB 1571075 A GB1571075 A GB 1571075A GB 1501512 A GB1501512 A GB 1501512A
Authority
GB
United Kingdom
Prior art keywords
organisms
micro
hydrocarbon
phosphatidic
solvent
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB1571075A
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
SCHWEDT PETROLCHEM K VEB
Original Assignee
SCHWEDT PETROLCHEM K VEB
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by SCHWEDT PETROLCHEM K VEB filed Critical SCHWEDT PETROLCHEM K VEB
Publication of GB1501512A publication Critical patent/GB1501512A/en
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/26—Processes using, or culture media containing, hydrocarbons
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/02—Separating microorganisms from their culture media

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • Microbiology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)

Abstract

1501512 Recovering micro-organisms from hydrocarbon fermentations PETROLCHEMISCHES KOMBINAT SCHWEDT VEB 16 April 1975 [29 April 1974] 15710/75 Heading C6F A process for the separation of micro-organisms e.g. yeast from a culture medium in the aerobic fermentation of the micro-organisms in liquid hydrocarbons in the presence of an aqueous nutrient solution involves emulsifying the culture medium and adding to the emulsion 0À1 to 5À0 g per kg of a separating agent which is a phosphatidic hydrocarbon extract obtained where microorganisms cultivated in a liquid hydrocarbon distillate are purified by extraction (see below), the micro-organisms being removed by phase separation after about 3 minutes. The biomass is recovered from the aqueous phase and dried, and the dried biomass is further purified by extraction with a solvent in order to remove any petroleum distillate still present. From the resulting extract after removal of the solvent by distillation or acetone precipitation there is obtained the solvent-free phosphatidic hydrocarbon extract for use as the separating agent in the process. In an Example, the phosphatidic hydrocarbon extract consisted of a 1% solution in petroleum distillate of 18À0% phosphatides of the lecithin type, 17À0% phosphatides of the kephalin type, 32À4% triglycerides, diglycerides, monoglycerides and free fatty acids, 0À6% steatin and ubiquinone, and 32À0% hydrocarbons.
GB1571075A 1974-04-29 1975-04-16 Process for the separation of micro-organisms from a culture medium Expired GB1501512A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DD17817074 1974-04-29

Publications (1)

Publication Number Publication Date
GB1501512A true GB1501512A (en) 1978-02-15

Family

ID=5495544

Family Applications (1)

Application Number Title Priority Date Filing Date
GB1571075A Expired GB1501512A (en) 1974-04-29 1975-04-16 Process for the separation of micro-organisms from a culture medium

Country Status (5)

Country Link
BG (1) BG22922A1 (en)
DE (1) DE2513354A1 (en)
FR (1) FR2268075A1 (en)
GB (1) GB1501512A (en)
RO (1) RO72527A (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2637579A1 (en) * 1975-10-31 1977-05-12 Petrolchemisches Kombinat ADDITIVE

Also Published As

Publication number Publication date
DE2513354A1 (en) 1975-11-06
RO72527A (en) 1983-07-07
FR2268075A1 (en) 1975-11-14
RO72527B (en) 1983-06-30
BG22922A1 (en) 1977-05-20

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Legal Events

Date Code Title Description
CSNS Application of which complete specification have been accepted and published, but patent is not sealed