CA2234965A1 - Methods for isolating polyhydroxyalkanoates from plants - Google Patents
Methods for isolating polyhydroxyalkanoates from plantsInfo
- Publication number
- CA2234965A1 CA2234965A1 CA 2234965 CA2234965A CA2234965A1 CA 2234965 A1 CA2234965 A1 CA 2234965A1 CA 2234965 CA2234965 CA 2234965 CA 2234965 A CA2234965 A CA 2234965A CA 2234965 A1 CA2234965 A1 CA 2234965A1
- Authority
- CA
- Canada
- Prior art keywords
- pha
- oil
- biomass
- phas
- plants
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Abstract
Methods are provided for separating polyhydroxyalkanoates ("PHAs") from plants, such as transgenic oil crop plants. The methods advantageously permit both the oil and the PHAs to be recovered from the plant biomass. To isolate the PHAs, in one embodiment, a biomass derived from an oil crop plant is pre-processed, for example by grinding, crushing or rolling. The oil then is extracted from the biomass with a first solvent in which the oil is soluble and in which the PHAs are not highly soluble to remove the oil. The biomass then can be extracted with a second solvent in which the PHA is soluble, to separate the PHA from the biomass. Alternatively, the PHA-containing biomass is treated with a chemical or biochemical agent, such as an enzyme, to chemically transform the PHA into a PHA derivative.
The PHA, derivative then is separated from the mixture using, for example, a physical separation process such as distillation, extraction or chromatography. Advantageously, using the method, the plant oils, the PHAs and PHA derivatives can be recovered and purified on a large scale from oil containing plants such as transgenic oil crop plants.
The PHA, derivative then is separated from the mixture using, for example, a physical separation process such as distillation, extraction or chromatography. Advantageously, using the method, the plant oils, the PHAs and PHA derivatives can be recovered and purified on a large scale from oil containing plants such as transgenic oil crop plants.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US548,840 | 1995-10-26 | ||
| US08/548,840 US6083729A (en) | 1995-10-26 | 1995-10-26 | Methods for isolating polyhydroxyalkanoates from plants |
| PCT/US1996/016921 WO1997015681A1 (en) | 1995-10-26 | 1996-10-23 | Methods for isolating polyhydroxyalkanoates from plants |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2234965A1 true CA2234965A1 (en) | 1997-05-01 |
| CA2234965C CA2234965C (en) | 2007-08-14 |
Family
ID=29406004
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA 2234965 Expired - Lifetime CA2234965C (en) | 1995-10-26 | 1996-10-23 | Methods for isolating polyhydroxyalkanoates from plants |
Country Status (1)
| Country | Link |
|---|---|
| CA (1) | CA2234965C (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7713720B2 (en) | 2002-08-06 | 2010-05-11 | Metabolix Inc. | Polymer extraction methods |
| US7781539B2 (en) | 2003-02-21 | 2010-08-24 | Metabolix Inc. | PHA blends |
| US7928167B2 (en) | 2003-02-21 | 2011-04-19 | Metabolix Inc. | PHA blends |
| US10030135B2 (en) | 2012-08-17 | 2018-07-24 | Cj Cheiljedang Corporation | Biobased rubber modifiers for polymer blends |
| US10611903B2 (en) | 2014-03-27 | 2020-04-07 | Cj Cheiljedang Corporation | Highly filled polymer systems |
| US10669417B2 (en) | 2013-05-30 | 2020-06-02 | Cj Cheiljedang Corporation | Recyclate blends |
-
1996
- 1996-10-23 CA CA 2234965 patent/CA2234965C/en not_active Expired - Lifetime
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7713720B2 (en) | 2002-08-06 | 2010-05-11 | Metabolix Inc. | Polymer extraction methods |
| US7981642B2 (en) | 2002-08-06 | 2011-07-19 | Metabolix, Inc. | Polymer extraction methods |
| US8003355B2 (en) | 2002-08-06 | 2011-08-23 | Metabolix, Inc. | Polymer extraction methods |
| US7781539B2 (en) | 2003-02-21 | 2010-08-24 | Metabolix Inc. | PHA blends |
| US7928167B2 (en) | 2003-02-21 | 2011-04-19 | Metabolix Inc. | PHA blends |
| US10030135B2 (en) | 2012-08-17 | 2018-07-24 | Cj Cheiljedang Corporation | Biobased rubber modifiers for polymer blends |
| US10669417B2 (en) | 2013-05-30 | 2020-06-02 | Cj Cheiljedang Corporation | Recyclate blends |
| US10611903B2 (en) | 2014-03-27 | 2020-04-07 | Cj Cheiljedang Corporation | Highly filled polymer systems |
Also Published As
| Publication number | Publication date |
|---|---|
| CA2234965C (en) | 2007-08-14 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request | ||
| MKEX | Expiry |
Effective date: 20161024 |