JPS5626193A - Removal of microbial cell from fermentation broth of long-chain dicarboxylic acid - Google Patents
Removal of microbial cell from fermentation broth of long-chain dicarboxylic acidInfo
- Publication number
- JPS5626193A JPS5626193A JP10162279A JP10162279A JPS5626193A JP S5626193 A JPS5626193 A JP S5626193A JP 10162279 A JP10162279 A JP 10162279A JP 10162279 A JP10162279 A JP 10162279A JP S5626193 A JPS5626193 A JP S5626193A
- Authority
- JP
- Japan
- Prior art keywords
- dicarboxylic acid
- long
- microbial cell
- chain dicarboxylic
- fermentation broth
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
PURPOSE: To make it possible to separate a long-chain dicarboxylic acid advantageously, by incubating a microorganism capable of producing the long-chain dicarboxylic acid in a culture medium of a straight chain hydrocarbon as a substrate, allowing the fermentation broth thus obtained to stand in a specific pH range, and centrifuging the broth to separate the microbial cell.
CONSTITUTION: A microorganism capable of producing a dicarboxylic acid is incubated in a culture medium of a straight chain hydrocarbon, e.g. n-tridecane, as a substrate, and the fermentation broth thus obtained is adjusted to pH9W12 with an alkili, e.g. NaOH or KOH, and allowed to stand at ordinary temperature for about 10hr. The broth is then centrifuged to separate the microbial cell from the hydrocarbon attached to the cell and other components. The separated fluid thus obtained is filtered through diatomaceous earth as a filter aid to give a clear filtrate, which is fed to the deposition step of the long-chain dicarboxylic acid. The separated solid content (mainly the diatomaceous earth) is circulated through the centrifuging step and reused. 95% or more of the microbial cell is separated to facilitate the purification of the long-chain dicarboxylic acid.
COPYRIGHT: (C)1981,JPO&Japio
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP10162279A JPS5626193A (en) | 1979-08-09 | 1979-08-09 | Removal of microbial cell from fermentation broth of long-chain dicarboxylic acid |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP10162279A JPS5626193A (en) | 1979-08-09 | 1979-08-09 | Removal of microbial cell from fermentation broth of long-chain dicarboxylic acid |
Publications (2)
Publication Number | Publication Date |
---|---|
JPS5626193A true JPS5626193A (en) | 1981-03-13 |
JPS5755399B2 JPS5755399B2 (en) | 1982-11-24 |
Family
ID=14305490
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP10162279A Granted JPS5626193A (en) | 1979-08-09 | 1979-08-09 | Removal of microbial cell from fermentation broth of long-chain dicarboxylic acid |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS5626193A (en) |
Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1049688C (en) * | 1995-10-05 | 2000-02-23 | 中国石油化工总公司 | Method for treatment of alpha, omega dibasic acid fermentation liquor |
US6218574B1 (en) | 1998-12-03 | 2001-04-17 | China Petrochemical Corporation | Process for purifying long-chain dicarboxylic acid |
CN1064951C (en) * | 1997-12-08 | 2001-04-25 | 中国石油化工总公司抚顺石油化工研究院 | Method for refining long-chain bibasic acid |
CN104529747A (en) * | 2014-12-26 | 2015-04-22 | 淄博广通化工有限责任公司 | Purification method of dodecanedioic acid |
CN104844440A (en) * | 2014-02-17 | 2015-08-19 | 上海凯赛生物技术研发中心有限公司 | Long-chain mixed dicarboxylic acid refining method |
CN104844441A (en) * | 2014-02-17 | 2015-08-19 | 上海凯赛生物技术研发中心有限公司 | Long-chain mixed dicarboxylic acid purification method |
-
1979
- 1979-08-09 JP JP10162279A patent/JPS5626193A/en active Granted
Cited By (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1049688C (en) * | 1995-10-05 | 2000-02-23 | 中国石油化工总公司 | Method for treatment of alpha, omega dibasic acid fermentation liquor |
CN1064951C (en) * | 1997-12-08 | 2001-04-25 | 中国石油化工总公司抚顺石油化工研究院 | Method for refining long-chain bibasic acid |
US6218574B1 (en) | 1998-12-03 | 2001-04-17 | China Petrochemical Corporation | Process for purifying long-chain dicarboxylic acid |
CN104844440A (en) * | 2014-02-17 | 2015-08-19 | 上海凯赛生物技术研发中心有限公司 | Long-chain mixed dicarboxylic acid refining method |
CN104844441A (en) * | 2014-02-17 | 2015-08-19 | 上海凯赛生物技术研发中心有限公司 | Long-chain mixed dicarboxylic acid purification method |
CN110256238A (en) * | 2014-02-17 | 2019-09-20 | 上海凯赛生物技术研发中心有限公司 | A kind of refining methd of long-chain mixed dibasic acid |
CN104529747A (en) * | 2014-12-26 | 2015-04-22 | 淄博广通化工有限责任公司 | Purification method of dodecanedioic acid |
Also Published As
Publication number | Publication date |
---|---|
JPS5755399B2 (en) | 1982-11-24 |
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