GB1459705A - Automated biuret analysis - Google Patents
Automated biuret analysisInfo
- Publication number
- GB1459705A GB1459705A GB1336575A GB1336575A GB1459705A GB 1459705 A GB1459705 A GB 1459705A GB 1336575 A GB1336575 A GB 1336575A GB 1336575 A GB1336575 A GB 1336575A GB 1459705 A GB1459705 A GB 1459705A
- Authority
- GB
- United Kingdom
- Prior art keywords
- reagent
- sample
- reaction vessel
- valve
- protein
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/02—Food
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
- G01N33/6827—Total protein determination, e.g. albumin in urine
- G01N33/683—Total protein determination, e.g. albumin in urine involving metal ions
- G01N33/6833—Copper, e.g. Folin-, Lowry-, biuret methods
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Chemical & Material Sciences (AREA)
- Immunology (AREA)
- Urology & Nephrology (AREA)
- Physics & Mathematics (AREA)
- Food Science & Technology (AREA)
- General Physics & Mathematics (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- Analytical Chemistry (AREA)
- Hematology (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Pathology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Biotechnology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biophysics (AREA)
- Bioinformatics & Computational Biology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
1459705 Biuret analysis COMMONWEALTH SCIENTIFIC & INDUSTRIAL RESEARCH. ORGANISATION 1 April 1975 [2 April 1974] 13365/75 Heading G1B A single-solution reagent for the colorimetric determination of protein comprises an aqueous biuret reagent (i.e. a reagent comprising potassium sodium tartrate, potassium hydroxide and a source of cupric ions which on contact with protein will give a violet coloration) and an aliphatic alcohol which is miscible with water. The alcohol is preferably isopropanol. Protein in a solution sample may be determined by heating said sample with the single-solution reagent, allowing colour to develop, separatingundissolved matter from the sample and determining the protein concentration by colorimetric measure- ment. A suitable apparatus for carrying out the determination comprises a heated and stirred reaction vessel, means for dispensing desired volumes of reagent and protein containing material to said reaction vessel, means for dispensing a predetermined volume of sample/reagent mixture from said reaction vessel to means adapted to clarify said mixture and dispense a clarified sample therefrom, wherein the passage of the mixture through the apparatus is controlled automatically. Preferably the apparatus includes means for dispensing reagent to said reaction vessel in an amount determined proportionately to the amount of proteinaceous sample. A suitable form of dispensing means is shown in Fig. 2 and comprises a beam 11 supported on a fulcrum 12, a sample holder 13 and reagent weighing vessel 15 being attached thereto. In operation, valve 19 opens, thus admitting reagent into vessel 15, the beam being consequently depressed until flag 17 interrupts light entering sensor 18, when valve 19 accordingly shuts and valve 20 opens, allowing reagent to syphon out of vessel 15 until flag 17 clears the light ray. The beam lock is engaged and the sample holder is then filled with sample. The lock is then disengaged and the beam falls until flag 17 interrupts the light ray, at which point valve 21- opens and reagent syphons into the reaction vessel until, as the beam rises, the flag clears the light ray and the valve 21 closes. Thus the amount of reagent dispensed to the reaction vessel will be in the same fixed proportion relative to the sample amount.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| AU712474 | 1974-04-02 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| GB1459705A true GB1459705A (en) | 1976-12-31 |
Family
ID=3697728
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| GB1336575A Expired GB1459705A (en) | 1974-04-02 | 1975-04-01 | Automated biuret analysis |
Country Status (3)
| Country | Link |
|---|---|
| JP (1) | JPS50161283A (en) |
| DE (1) | DE2513840A1 (en) |
| GB (1) | GB1459705A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114755195A (en) * | 2022-06-14 | 2022-07-15 | 浙江省立同德医院(浙江省精神卫生研究院) | Preparation method of reagent for quickly detecting urine protein |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4132528A (en) * | 1978-01-03 | 1979-01-02 | Eastman Kodak Company | Analytical element for the analysis of liquids under high pH conditions |
| HU183928B (en) * | 1982-05-19 | 1984-06-28 | Mezoegazdasagi Es Elelmiszerip | Process and apparatus for genetical characterizing and qualifying plants, first of all sorts of cultivated plants |
| RU2473699C2 (en) * | 2011-04-06 | 2013-01-27 | Государственное образовательное учреждение высшего профессионального образования "Забайкальский государственный гуманитарно-педагогический университет им. Н.Г. Чернышевского" | Quantitative food proteins content determination method |
-
1975
- 1975-03-27 DE DE19752513840 patent/DE2513840A1/en not_active Withdrawn
- 1975-04-01 JP JP3866075A patent/JPS50161283A/ja active Pending
- 1975-04-01 GB GB1336575A patent/GB1459705A/en not_active Expired
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114755195A (en) * | 2022-06-14 | 2022-07-15 | 浙江省立同德医院(浙江省精神卫生研究院) | Preparation method of reagent for quickly detecting urine protein |
Also Published As
| Publication number | Publication date |
|---|---|
| DE2513840A1 (en) | 1975-10-23 |
| JPS50161283A (en) | 1975-12-27 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PS | Patent sealed | ||
| PCNP | Patent ceased through non-payment of renewal fee |