GB1435400A - Process for the activation of cholesterol oxidase - Google Patents
Process for the activation of cholesterol oxidaseInfo
- Publication number
- GB1435400A GB1435400A GB5175774A GB5175774A GB1435400A GB 1435400 A GB1435400 A GB 1435400A GB 5175774 A GB5175774 A GB 5175774A GB 5175774 A GB5175774 A GB 5175774A GB 1435400 A GB1435400 A GB 1435400A
- Authority
- GB
- United Kingdom
- Prior art keywords
- cholesterol
- cholesterol oxidase
- lipophilic
- detecting
- active compound
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/60—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving cholesterol
Abstract
Cholesterol oxidase is activated by adding a surface-active compound with lipophilic and hydrophilic properties. This method is advantageously used for detecting and for determining cholesterol and cholesterol esters in body fluids by oxidising the cholesterol with oxygen in the presence of cholesterol oxidase to cholestenone and hydrogen peroxide and detecting the hydrogen peroxide by peroxidase-catalysed oxidation of an oxidation catalyst, wherein the cholesterol oxidase is activated before it is used by adding a surface-active compound with lipophilic and hydrophilic properties. The diagnostic composition for carrying out this method is distinguished by consisting of an absorbent carrier or a plastic film which is impregnated with cholesterol oxidase, a system for detecting H2O2, buffer and at least one surface-active compound with lipophilic and hydrophilic properties, or contains these embedded in it.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE2361169A DE2361169C3 (en) | 1973-12-07 | 1973-12-07 | Process for the activation of cholesterol oxidase freed from detergent traces |
| DE742439348A DE2439348C3 (en) | 1973-12-07 | 1974-08-16 | Diagnostic agent for the detection and determination of cholesterol and cholesterol esters in body fluids |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| GB1435400A true GB1435400A (en) | 1976-05-12 |
Family
ID=25766212
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| GB5175774A Expired GB1435400A (en) | 1973-12-07 | 1974-11-29 | Process for the activation of cholesterol oxidase |
Country Status (18)
| Country | Link |
|---|---|
| JP (1) | JPS5733954B2 (en) |
| AR (1) | AR201067A1 (en) |
| AT (1) | AT338441B (en) |
| BE (1) | BE822843A (en) |
| CA (1) | CA1044582A (en) |
| CH (1) | CH613046A5 (en) |
| DD (1) | DD115759A5 (en) |
| DE (2) | DE2361169C3 (en) |
| DK (1) | DK136293C (en) |
| FI (1) | FI57616C (en) |
| FR (1) | FR2254026B1 (en) |
| GB (1) | GB1435400A (en) |
| HK (1) | HK49279A (en) |
| HU (1) | HU170109B (en) |
| IL (1) | IL46073A (en) |
| IT (1) | IT1026827B (en) |
| NL (2) | NL170022C (en) |
| SE (2) | SE7415324L (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4275152A (en) | 1977-02-03 | 1981-06-23 | Eastman Kodak Company | Hydrolysis of protein-bound cholesterol esters |
| US4275151A (en) | 1977-02-03 | 1981-06-23 | Eastman Kodak Company | Hydrolysis of protein-bound cholesterol esters |
| US4298345A (en) | 1977-11-21 | 1981-11-03 | Damon Corporation | Method and apparatus for chemical spot test analysis |
| US4409326A (en) * | 1980-07-10 | 1983-10-11 | Modrovich Ivan Endre | Stabilized enzymatic solutions and method for determining total cholesterol in human serum |
| US4554249A (en) * | 1982-02-18 | 1985-11-19 | Amano Pharmaceutical Company Limited | Method for the quantitative determination of physiological components in biological fluids |
| US5047327A (en) * | 1982-04-02 | 1991-09-10 | Ivan E. Modrovich | Time-stable liquid cholesterol assay compositions |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| IL48804A (en) * | 1975-01-29 | 1979-05-31 | Baxter Travenol Lab | Imminological reagent comprising a mixture of polyethyleneglycol and a nonionic surfactant |
| DE2612725C3 (en) * | 1976-03-25 | 1979-05-03 | Boehringer Mannheim Gmbh, 6800 Mannheim | Method and reagent for the determination of cholesterol |
| US4161425A (en) * | 1976-07-01 | 1979-07-17 | Beckman Instruments, Inc. | Enzymatic reagent system for total cholesterol assay using oxygen-rate method |
| CA1104041A (en) * | 1977-02-03 | 1981-06-30 | Charles T. Goodhue | Hydrolysis of protein-bound cholesterol esters |
| JPS5791197A (en) * | 1980-11-20 | 1982-06-07 | Toyobo Co Ltd | Reagent for measuring activity of substrate or enzyme |
| FR2556010B1 (en) * | 1982-02-18 | 1988-07-29 | Amano Pharma Co Ltd | METHOD FOR THE QUANTITATIVE DETERMINATION OF PHYSIOLOGICAL COMPONENTS IN BIOLOGICAL FLUIDS |
| DE3374019D1 (en) * | 1982-07-23 | 1987-11-12 | Wako Pure Chem Ind Ltd | Process for quantitative determination of substrate treated with oxidase |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| IT988069B (en) * | 1971-09-22 | 1975-04-10 | Nat Res Dev | ENZYMATIC PREPARATION AND METHOD FOR ITS PRODUCTION |
-
1973
- 1973-12-07 DE DE2361169A patent/DE2361169C3/en not_active Expired
-
1974
- 1974-08-16 DE DE742439348A patent/DE2439348C3/en not_active Expired
- 1974-11-13 CA CA213,624A patent/CA1044582A/en not_active Expired
- 1974-11-15 AR AR256556A patent/AR201067A1/en active
- 1974-11-18 IL IL46073A patent/IL46073A/en unknown
- 1974-11-19 NL NLAANVRAGE7415037,A patent/NL170022C/en not_active IP Right Cessation
- 1974-11-29 GB GB5175774A patent/GB1435400A/en not_active Expired
- 1974-11-29 FR FR7439225A patent/FR2254026B1/fr not_active Expired
- 1974-12-02 JP JP13826074A patent/JPS5733954B2/ja not_active Expired
- 1974-12-02 BE BE151061A patent/BE822843A/en unknown
- 1974-12-04 DD DD182779A patent/DD115759A5/xx unknown
- 1974-12-04 HU HUBO1525A patent/HU170109B/hu unknown
- 1974-12-04 FI FI3513/74A patent/FI57616C/en active
- 1974-12-05 AT AT974074A patent/AT338441B/en not_active IP Right Cessation
- 1974-12-05 CH CH1619474A patent/CH613046A5/en not_active IP Right Cessation
- 1974-12-05 IT IT30230/74A patent/IT1026827B/en active
- 1974-12-06 DK DK637074A patent/DK136293C/en active
- 1974-12-06 SE SE7415324A patent/SE7415324L/ not_active Application Discontinuation
-
1979
- 1979-07-19 HK HK492/79A patent/HK49279A/en unknown
-
1981
- 1981-06-15 SE SE8103735A patent/SE8103735L/en not_active Application Discontinuation
-
1982
- 1982-03-31 NL NL8201349A patent/NL8201349A/en not_active Application Discontinuation
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4275152A (en) | 1977-02-03 | 1981-06-23 | Eastman Kodak Company | Hydrolysis of protein-bound cholesterol esters |
| US4275151A (en) | 1977-02-03 | 1981-06-23 | Eastman Kodak Company | Hydrolysis of protein-bound cholesterol esters |
| US4298345A (en) | 1977-11-21 | 1981-11-03 | Damon Corporation | Method and apparatus for chemical spot test analysis |
| US4409326A (en) * | 1980-07-10 | 1983-10-11 | Modrovich Ivan Endre | Stabilized enzymatic solutions and method for determining total cholesterol in human serum |
| US4554249A (en) * | 1982-02-18 | 1985-11-19 | Amano Pharmaceutical Company Limited | Method for the quantitative determination of physiological components in biological fluids |
| US5047327A (en) * | 1982-04-02 | 1991-09-10 | Ivan E. Modrovich | Time-stable liquid cholesterol assay compositions |
Also Published As
| Publication number | Publication date |
|---|---|
| IL46073A (en) | 1977-05-31 |
| NL170022C (en) | 1982-09-16 |
| NL7415037A (en) | 1975-06-10 |
| DE2439348A1 (en) | 1976-03-04 |
| FI57616B (en) | 1980-05-30 |
| DK637074A (en) | 1975-07-28 |
| DE2439348B2 (en) | 1977-09-15 |
| FI57616C (en) | 1980-09-10 |
| AU7557874A (en) | 1976-05-20 |
| DK136293C (en) | 1978-02-20 |
| SE8103735L (en) | 1981-06-15 |
| DE2361169B2 (en) | 1978-01-19 |
| IT1026827B (en) | 1978-10-20 |
| DE2361169C3 (en) | 1978-09-14 |
| NL8201349A (en) | 1982-08-02 |
| DE2361169A1 (en) | 1975-06-19 |
| JPS5733954B2 (en) | 1982-07-20 |
| FR2254026A1 (en) | 1975-07-04 |
| HU170109B (en) | 1977-04-28 |
| CA1044582A (en) | 1978-12-19 |
| DE2439348C3 (en) | 1979-03-01 |
| AT338441B (en) | 1977-08-25 |
| AR201067A1 (en) | 1975-02-06 |
| SE7415324L (en) | 1975-06-09 |
| IL46073A0 (en) | 1975-02-10 |
| FR2254026B1 (en) | 1977-11-04 |
| JPS5089588A (en) | 1975-07-18 |
| HK49279A (en) | 1979-07-27 |
| DK136293B (en) | 1977-09-26 |
| DD115759A5 (en) | 1975-10-12 |
| NL170022B (en) | 1982-04-16 |
| CH613046A5 (en) | 1979-08-31 |
| BE822843A (en) | 1975-06-02 |
| FI351374A (en) | 1975-06-08 |
| ATA974074A (en) | 1976-12-15 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PS | Patent sealed [section 19, patents act 1949] | ||
| PE20 | Patent expired after termination of 20 years |
Effective date: 19941128 |